Intron 4 VNTR (4a/b) Polymorphism of the Endothelial Nitric Oxide Synthase Gene Is Associated with Breast Cancer in Mexican Women

Ramírez-Patiño Ramiro,Figuera Luis Eduardo,Puebla-Pérez Ana María,Delgado-Saucedo Jorge Ivan,Legazpi-Macias María Magdalena,Mariaud-Schmidt Rocio Patricia,Ramos-Silva Adriana,Gutiérrez-Hurtado Itzae A 대한의학회 2013 Journal of Korean medical science Vol.28 No.11

The endothelial nitric oxide synthase (eNOS) gene plays an important role in several biological functions. Polymorphisms of the eNOS gene have been associated with cancer. It has been suggested that the VNTR 4 a/b polymorphism may affect the expression of eNOS and contributes to tumor promotion in the mammary gland. We examined the role of the eNOS4 a/b polymorphism by comparing the genotypes of 281 healthy Mexican women with the genotypes of 429 Mexican women with breast cancer (BC). The observed genotype frequencies for control and BC patients were 0.6% and 0.7% for a/a (polymorphic); 87% and 77% for a/a (wild type); and 12% and 22% for a/b respectively. We found that the odds ratio (OR) was 1.9, with a 95% confidence interval (95%CI) of 1.29-2.95, P=0.001 for genotypes a/a-a/b, b/c. The association was also evident when comparing the distribution of the a/a-a/b genotypes in patients with high levels of glutamate-oxaloacetate transaminase (SGOT) (OR, 1.93; 95% CI, 1.14-3.28; P=0.015); undergoing menopause with high levels of SGOT (OR, 2.0; 95% CI, 1.1-3.84); and with high levels of glutamic-pyruvic transaminase (SGPT) (OR, 3.5; 95% CI, 1.56-8.22). The genotypes a/a-a/b are associated with BC susceptibility in the analyzed samples from the Mexican population.

AURKA Gene Variants rs1047972, and rs8173 Are Associated With Breast Cancer

Eric Jonathan Maciel-Cruz,Luis Eduardo Figuera-Villanueva,Asbiel Felipe Garibaldi-Ríos,Belinda Claudia Gómez-Meda,Guillermo Moisés Zúñiga-González,Ana María Pérez,Paola B Castro-García,Ramiro Ramírez- 한국유방암학회 2023 Journal of breast cancer Vol.26 No.4

Purpose: Association between variants rs1047972 and rs8173 of the AURKA gene in healthy women and breast cancer (BC) in a Mexican population. Methods: Genomic DNA samples from 409 healthy women and 572 patients with BC were analyzed for variants rs1047972 and rs8173 of the AURKA gene by polymerase chain reaction-restriction fragment length polymorphism. Results: TT genotype (odds ratio [OR], 2.5; 95% confidence interval [CI], 1.22–5.11; p = 0.0015) and the T allele (OR, 1.16; 95% CI, 1.23–2.12; p = 0.0007) of the rs1047972 variant were associated as risk susceptibility for BC relative to the control group. Contrarily, the GG genotype (OR, 0.64; 95% CI, 0.43–0.94; p = 0.029) was associated as a protective factor of susceptibility of BC of the variant rs8173 of the AURKA gene. Differences were observed in the patients with BC who were carriers of the CT genotype of the rs1047972 variant with overweight, obesity, estrogen receptor-positive plus obesity, Ki-67 (≥ 20%) plus history familial positive of cancer; and for variant rs8173 the BC patients who were CG carriers and presented chemotherapy gastric toxicity, hormonal receptor positive plus chemotherapy gastric toxicity, and menopause status plus chemotherapy gastric toxicity (p < 0.05). Two common haplotypes were identified in the study groups: CG and TC genotypes, were associated as a protective and risk factor, respectively (p < 0.05). Conclusion: Variants rs1047972 and rs8173 of the AURKA gene and the TC haplotype were associated as risk susceptibility factors for BC in this population.

Fluoroscopic Characterization of Colonic Dysmotility Associated to Opioid and Cannabinoid Agonists in Conscious Rats

Susana Díaz-Ruano,Ana E López-Pérez,Rocío Girón,Irene Pérez-García,María I Martín-Fontelles,Raquel Abalo 대한소화기 기능성질환∙운동학회 2019 Journal of Neurogastroenterology and Motility (JNM Vol.25 No.2

Background/Aims Gastrointestinal adverse effects have a major impact on health and quality of life in analgesics users. Non-invasive methods to study gastrointestinal motility are of high interest. Fluoroscopy has been previously used to study gastrointestinal motility in small experimental animals, but they were generally anesthetized and anesthesia itself may alter motility. In this study, our aim is to determine, in conscious rats, the effect of increasing doses of 2 opioid (morphine and loperamide) and 1 cannabinoid (WIN 55,212-2) agonists on colonic motility using fluoroscopic recordings and spatio-temporal maps. Methods Male Wistar rats received barium sulfate intragastrically, 20–22 hours before fluoroscopy, so that stained fecal pellets could be seen at the time of recording. Animals received an intraperitoneal administration of morphine, loperamide, or WIN 55,212-2 (at 0.1, 1, 5, or 10 mg/kg) or their corresponding vehicles (saline, Cremophor, and Tocrisolve, respectively), 30 minutes before fluoroscopy. Rats were conscious and placed within movement-restrainers for the length of fluoroscopic recordings (120 seconds). Spatio-temporal maps were built, and different parameters were analyzed from the fluoroscopic recordings in a blinded fashion to evaluate colonic propulsion of endogenous fecal pellets. Results The analgesic drugs inhibited propulsion of endogenous fecal pellets in a dose-dependent manner. Conclusions Fluoroscopy allows studying colonic propulsion of endogenous fecal pellets in conscious rats. Our method may be applied to the noninvasive study of the effect of different drug treatments and pathologies. Background/Aims Gastrointestinal adverse effects have a major impact on health and quality of life in analgesics users. Non-invasive methods to study gastrointestinal motility are of high interest. Fluoroscopy has been previously used to study gastrointestinal motility in small experimental animals, but they were generally anesthetized and anesthesia itself may alter motility. In this study, our aim is to determine, in conscious rats, the effect of increasing doses of 2 opioid (morphine and loperamide) and 1 cannabinoid (WIN 55,212-2) agonists on colonic motility using fluoroscopic recordings and spatio-temporal maps. Methods Male Wistar rats received barium sulfate intragastrically, 20–22 hours before fluoroscopy, so that stained fecal pellets could be seen at the time of recording. Animals received an intraperitoneal administration of morphine, loperamide, or WIN 55,212-2 (at 0.1, 1, 5, or 10 mg/kg) or their corresponding vehicles (saline, Cremophor, and Tocrisolve, respectively), 30 minutes before fluoroscopy. Rats were conscious and placed within movement-restrainers for the length of fluoroscopic recordings (120 seconds). Spatio-temporal maps were built, and different parameters were analyzed from the fluoroscopic recordings in a blinded fashion to evaluate colonic propulsion of endogenous fecal pellets. Results The analgesic drugs inhibited propulsion of endogenous fecal pellets in a dose-dependent manner. Conclusions Fluoroscopy allows studying colonic propulsion of endogenous fecal pellets in conscious rats. Our method may be applied to the noninvasive study of the effect of different drug treatments and pathologies.

Composition and Antioxidant Activity of Trigona carbonaria Honey from Australia

Livia Persano Oddo,Tim A. Heard,Antonio Rodríguez-Malaver,Rosa Ana Pérez,Miguel Fernández-Muiño,María Teresa Sancho,Giulio Sesta,Lorenzo Lusco,Patricia Vit 한국식품영양과학회 2008 Journal of medicinal food Vol.11 No.4

Stingless bees (Tribe Meliponini) are a diverse group of highly eusocial bees distributed throughout the tropics and subtropics. Trigona carbonaria honey, from Australia, was characterized by traditional physicochemical parameters (acidity, sugars, diastase, electrical conductivity, hydroxymethylfurfural, invertase, nitrogen, and water content) and other compositional factors (flavonoids, polyphenols, organic acids, and water activity), as well as total antioxidant capacity and radical scavenging activity. For the Australian T. carbonaria, the traditional analytical parameters were similar to those previously reported for neotropical stingless bee honey and confirm that honeys produced by Meliponini bees possess several physicochemical properties that are distinctly different from Apis mellifera honey, with higher values of moisture (26.5 ± 0.8 g of water/100 g of honey), water activity (0.74 ± 0.01), electrical conductivity (1.64 ± 0.12 mS/cm), and free acidity (124.2 ± 22.9 mEq/kg of honey) and a very low diastase activity (0.4 ± 0.5 diastase number) and invertase activity (5.7 ± 1.5 invertase number). The sugar spectrum was quite different from that of A. mellifera honey, with 20.3 ± 2.9 g of maltose/100 g of honey. The values of pH (4.0 ± 0.1), lactonic acidity (4.7 ± 0.8 mEq/kg of honey), sucrose (1.8 ± 0.4 g/100 g of honey), and fructose/glucose ratio (1.42 ± 0.13) fell in the same ranges as those of A. mellifera honey. Citric (0.23 ± 0.09) and malic (0.12 ± 0.03) acid concentrations (in g/kg of honey) of T. carbonaria honeys were in the range described for A. mellifera honey. D-Gluconic was more concentrated (9.9 ± 1.3 g/kg of honey), in the range of Italian Castanea, Thymus, Arbutus, and honeydew honeys. Flavonoid content was 10.02 ± 1.59 mg of quercetin equivalents/100 g of honey, and polyphenol contents were 55.74 ± 6.11 mg of gallic acid equivalents/100 g of honey. The antioxidant activity, expressed as percentage of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) cation (ABTS.+) decolorization, was 233.96 ± 50.95 μM Trolox equivalents, and free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH.) depletion was 48.03 ± 12.58 equivalents of ascorbic acid. All reported values are averages ± standard deviation. The antioxidant activity can represent an important added value for T. carbonaria honey, to initiate a medicinal approach for both nutritional and pharmaceutical applications, besides further physicochemical characterization.

EXPLORING GENDER DIVERSITY THROUGH FASHION JOURNALISM. A COMPARATIVE STUDY OF VOGUE COVERAGE ON THE RED CARPETS OF US AND EUROPEAN CINEMA

Ana María,Concha Pérez Curiel,Jorge Zarauza 글로벌지식마케팅경영학회 2021 Global Fashion Management Conference Vol.2021 No.11

Effect of Ditaxin and Heteranthin and Inhibitory Effect of Ditaxis heterantha Extract on L5178Y Tumor Development in Mice

María Eugenia Jaramillo-Flores,Herry Heriyati Permady,Ana Maria Puebla-Pérez,Eduardo Padilla,Eugenia del Carmen Lugo-Cervantes,Jorge Ivan Delgado-Saucedo,Eva Ramón-Gallegos 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.5

Ditaxis heterantha seeds are used as spices for flavoring and coloring food. Two new apocarotenoids derived from the seeds, heteranthin and ditaxin, were evaluated for their in vitro cytotoxic effects in murine lymphoma cells lines. Bioabsorption in mice and preventive and antitumor effects of the apocarotenoids were determined. Ditaxin and heteranthin showed cytotoxic effects in vitro against murine malignant cells and normal splenocyte cells. The 50% inhibitory concentration (IC_50) for ditaxin in splenocytes was 0.1825 mM; in L5178Y, the IC_50 was 0.1923 mM. The heteranthin IC_50 in splenocytes was 0.1325 mM; in L5178Y, the value was 0.3889 mM. The maximum ditaxin plasma concentration was found after 2 hours of administration (mean±standard deviation, 7.5±2.05 μg/mL). Oral administration of the D. heterantha extract (100 mg/kg per day) for 14 days after the L5178Y lymphoma cell implantation showed no significant effect compared with groups that were not pretreated. However, tumor inhibition in groups treated intraperitoneally before inoculation with the L5178Y cells showed a significant difference (P<.001) compared with the groups not pretreated.