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Zhang Jie,Jiang Dun,Dong Xiaowen,Meng Zhaojun,Yan Shanchun 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.4
To investigate the accumulation patterns of Cd and/or Pb in various body parts, organs and tissues of the Asian gypsy moth (Lymantria dispar) larvae under Cd and/or Pb stress, Cd and Pb treated artificial diets were used to feed the larvae in the current study. These larval body parts/organs/tissues included the heads, integuments (body walls), alimentary canals, fat bodies and hemolymphs. Our results showed that under Cd and/or Pb stress, their accumulations in larval body parts/organs/tissues were significantly higher than those in the control, with the amounts of tested metals in the fat bodies and hemolymphs and alimentary canals being significantly higher than those in the heads and integuments. Under the single Cd (0, 7.5, 10.5 mg/kg) or Pb stress (0, 55, 90, 125 mg/kg), the accumulations of these heavy metals were positively correlated with their concentrations in diets. Under the combined stress (Cd × Pb), the Cd accumulation at the lower Pb concentrations was higher than that at higher Pb concentrations for the body parts/organs/tissues, similar effects of Cd concentrations on Pb accumulations were also recorded. These results clearly showed that Cd and/or Pb were accumulated in various body parts/organs/tissues at different levels under the heavy metal stress. And accumulations of Pb/Cd were positively correlated with their concentrations in the diets under the single stress. Under the combined stresses, Cd and Pb had synergistic effects at low concentrations whereas antagonistic effects at high concentrations. The accumulations of Cd and/or Pb in the gypsy moth larvae affects normal physiological and biochemical functions, and thus affects their growth and development.
Dun, Yaoyan,Liu, Min,Chen, Jing,Peng, Danli,Zhao, Haixia,Zhou, Zhiyong,Wang, Ting,Liu, Chaoqi,Guo, Yuhui,Zhang, Changcheng,Yuan, Ding The Korean Society of Ginseng 2018 Journal of Ginseng Research Vol.42 No.1
Background: Saponins from Panax japonicus (SPJ) are the most abundant and main active components of P. japonicus, which replaces ginseng roots in treatment for many kinds of diseases in the minority ethnic group in China. Our previous studies have demonstrated that SPJ has the effects of anti-inflammation through the mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-${\kappa}B$) signaling pathways. The present study was designed to investigate whether SPJ can modulate intestinal tight junction barrier in aging rats and further to explore the potential mechanism. Methods: Aging rats had been treated with different doses (10 mg/kg, 30 mg/kg, and 60 mg/kg) of SPJ for 6 mo since they were 18 mo old. After the rats were euthanized, the colonic samples were harvested. Levels of tight junctions (claudin-1 and occludin) were determined by immunohistochemical staining. Levels of proinflammatory cytokines (interleukin-$1{\beta}$ and tumor necrosis factor-${\alpha}$) were examined by Western blot. NF-${\kappa}B$ and phosphorylation of MAPK signaling pathways were also determined by Western blot. Results: We found that SPJ increased the expression of the tight junction proteins claudin-1 and occludin in the colon of aging rats. Treatment with SPJ decreased the levels of interleukin-$1{\beta}$ and tumor necrosis factor-${\alpha}$, reduced the phosphorylation of three MAPK isoforms, and inhibited the expression of NF-${\kappa}B$ in the colon of aging rats. Conclusion: The studies demonstrated that SPJ modulates the damage of intestinal epithelial tight junction in aging rats, inhibits inflammation, and downregulates the phosphorylation of the MAPK and $NF-{\kappa}B$ signaling pathways.
Dun Wu,Wenyong Zhang 한국지질과학협의회 2019 Geosciences Journal Vol.23 No.6
This paper reports systematically the redox sensitive elements (Mo, V, Cd, Cr, U and Th) and carbon and oxygen isotope data of the Late Carboniferous Taiyuan Formation limestone in the Huainan Coalfield in the southern margin of North China Platform, combined with the observation of limestone slice, and tries to explain the sedimentary environment of each layer of limestone. Through the analysis of the diagenesis of each layer of limestone, except for L7 and L12 limestone, the lithogenesis change of the remaining limestone becomes weak, which generally reflects the geochemical characteristics of the paleosol during sedimentation. There are three negative shifts of δ13C in Taiyuan Formation limestone, which may be related to the enrichment of redox sensitive elements (Cd, Cr, Mo and V) in limestone. In addition, the value of the above redox sensitive elements shows a negative drift. Combining the low V/Cr ratio and high Th/U ratio, it can be concluded that limestone of the Taiyuan Formation is likely to be deposited under an oxidizing condition.
Xinyue Zhang,Bushi Dai,Shucheng Ren,Zenan Hu,Xin Zheng,Yao Wang,Hongbin Sun,Dun Niu,Linshan Wang 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.5
Due to the complexity of the contaminations in wastewater, a universal adsorbent that takes many sorts of pollutant is desired. A multi-functioning magnetic Fe3O4-cored magnesium-aluminum layered double oxide (Fe3O4@LDO) composite was prepared and employed as an adsorbent for removing F-, Sb(III) and MO from water, respectively, which are three typical contaminants with different molecule sizes as water contamination. The effect of pH contact time and initial concentrations of pollutants on the removal rates of each contaminant were investigated. Fe3O4@LDO composite showed excellent adsorption performance for all of the three molecules F-, Sb(III) and MO, and their maximum adsorption capacities are as high as 258, 391, and 506mg/g, respectively. The adsorption process of F-, Sb(III) and MO on Fe3O4@LDO fitted well to Langmuir model and pseudo-second-order kinetic model. The adsorption mechanisms of F-, Sb(III) and MO on Fe3O4@LDO were investigated. We discovered that iron ions diffused from Fe3O4 nanospheres contributed to the good performance of the absorbent.
Helicoverpa armigera Nucleopolyhedrovirus ORF80 Encodes a Late, Nonstructural Protein
Wang, Dun,Zhang, Chuan-Xi Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.1
The Helicoverpa armigera nucleopolyhedrovirus (HearNPV) ORF80 (ha80) has 765 bp encoding a protein with approximately 254 amino acids and a predicted molecular weight of 30.8 kDa. Homologues of ha80 are found in most baculovirus sequences, including those from lepidopteran NPVs, lepidopteran granuloviruses (GVs), hymenopteran baculoviruses, and one dipteran baculovirus, yet their functions remain unclear. In this study we characterized ha80, and showed that it was transcribed late in infected host cells (HzAM1). The product of ha80 was a 31 kDa protein that was not a structural protein of budded virus (BV) or occlusion-derived virus (ODV) particles. Ha80 was first detected in the cytoplasm of infected HzAM1 cells at 12 h p.i., and was observed in the nucleus at later stages of infection, suggesting that it may be involved in transporting viral proteins into the host cell nucleus or play its roles in the nucleus.
Effects of Cavity Size and Density on Polymer Micro Hot Embossing
Xiang Zhang,Ge Fang,Tengjiao Jiang,Na Zhao,Junfeng Li,Bowen Dun,Qian Li 한국정밀공학회 2015 International Journal of Precision Engineering and Vol. No.
We designed nine micro molds with various cavity geometric parameters to investigate their effects on micropillars replication of the amorphous polymer via micro hot embossing. Picoseconds laser was used to fabricate the micro holes array on the stainless steel plates with 200 μm thickness as micro molds. The mechanical behavior of PMMA above glass transition temperature was investigated by uniaxial compression tests at various temperatures and strain rates, the results were used in simulation as constitutive relation. Confocal microscope and optical microscope showed that the replication height has a complicate nonlinear relationship with cavity size and density. DEFORM 3D was used to model and simulate the embossing process, and it is a good approach to investigate the micropallars replication heights, front angles and filling ratios.
Wu Dun,Li Bo,Jia Cheng,Dong Min,Liang Wenxu,Gao Xia,Zhang Hui 한국탄소학회 2024 Carbon Letters Vol.34 No.3
In recent years, the efficient and clean utilization of coal has been widely concerned by scholars at home and abroad. Despite the abundance of global coal resources, the deep utilization rate of coal is still insufficient. To address this challenge, it has been explored the development and preparation of coal-based high value-added carbonaceous materials. In the present study, a novel process was developed for the preparation of graphene using biphenyl sourced from low-rank coal. Using chemical vapor deposition (CVD) technology, it was successfully implemented for us to grow high-quality graphene on copper foils. The prepared graphene products were observed and characterized using Raman spectroscopy, optical microscopy and scanning electron microscopy techniques. The results of this research provide a new perspective for the utilization of low-rank coal resources.
Xuhui Zhang,Yi-Min Xia,Qing Tan,Dun Wu**** 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.10
To improve the excavation efficiency of Tunnel Boring Machines (TBMs), the free-face-assisted rock cutting method induced by TBM disc cutter (FM) was explored and the differences between the FM and the conventional Cutting Method (CM) were also compared based on a series of rock cutting tests. The results show that when the free face distance is less than a critical value, the FM can promote the tensile failure of the rock and the formation of big rock debris effectively. Moreover, the cutting efficiency and the cutting forces including normal force, rolling force and side force of the disc cutter under the FM are also significantly reduced and improved, respectively, compared to that under the CM. The FM can be applied in multi-stage cutterhead TBMs to improve the cutting efficiency and reduce the failure of the disc cutter, the research results indicate that the free face distance should be controlled within 80 mm to use the free face effectively.