Profiling of remote skeletal muscle gene changes resulting from stimulation of atopic dermatitis disease in NC/Nga mouse model

Donghee Lee,Yelim Seo,Young-Won Kim,Seongtae Kim,Jeongyoon Choi,Sung-Hee Moon,Hyemi Bae,Hui-sok Kim,Hangyeol Kim,Jae-Hyun Kim,Tae-Young Kim,Eunho Kim,Suemin Yim,Inja Lim,Hyoweon Bang,Jung-Ha Kim,Jae-H 대한약리학회 2019 The Korean Journal of Physiology & Pharmacology Vol.23 No.5

Although atopic dermatitis (AD) is known to be a representative skin disorder, it also affects the systemic immune response. In a recent study, myoblasts were shown to be involved in the immune regulation, but the roles of muscle cells in AD are poorly understood. We aimed to identify the relationship between mitochondria and atopy by genome-wide analysis of skeletal muscles in mice. We induced AD-like symptoms using house dust mite (HDM) extract in NC/Nga mice. The transcriptional profiles of the untreated group and HDM-induced AD-like group were analyzed and compared using microarray, differentially expressed gene and functional pathway analyses, and protein interaction network construction. Our microarray analysis demonstrated that immune response-, calcium handling-, and mitochondrial metabolism-related genes were differentially expressed. In the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology pathway analyses, immune response pathways involved in cytokine interaction, nuclear factor-kappa B, and T-cell receptor signaling, calcium handling pathways, and mitochondria metabolism pathways involved in the citrate cycle were significantly upregulated. In protein interaction network analysis, chemokine family-, muscle contraction process-, and immune response-related genes were identified as hub genes with many interactions. In addition, mitochondrial pathways involved in calcium signaling, cardiac muscle contraction, tricarboxylic acid cycle, oxidation-reduction process, and calcium-mediated signaling were significantly stimulated in KEGG and Gene Ontology analyses. Our results provide a comprehensive understanding of the genome-wide transcriptional changes of HDM-induced AD-like symptoms and the indicated genes that could be used as AD clinical biomarkers.

Profiling of remote skeletal muscle gene changes resulting from stimulation of atopic dermatitis disease in NC/Nga mouse model

Lee, Donghee,Seo, Yelim,Kim, Young-Won,Kim, Seongtae,Choi, Jeongyoon,Moon, Sung-Hee,Bae, Hyemi,Kim, Hui-sok,Kim, Hangyeol,Kim, Jae-Hyun,Kim, Tae-Young,Kim, Eunho,Yim, Suemin,Lim, Inja,Bang, Hyoweon,Ki The Korean Society of Pharmacology 2019 The Korean Journal of Physiology & Pharmacology Vol.23 No.5

Although atopic dermatitis (AD) is known to be a representative skin disorder, it also affects the systemic immune response. In a recent study, myoblasts were shown to be involved in the immune regulation, but the roles of muscle cells in AD are poorly understood. We aimed to identify the relationship between mitochondria and atopy by genome-wide analysis of skeletal muscles in mice. We induced AD-like symptoms using house dust mite (HDM) extract in NC/Nga mice. The transcriptional profiles of the untreated group and HDM-induced AD-like group were analyzed and compared using microarray, differentially expressed gene and functional pathway analyses, and protein interaction network construction. Our microarray analysis demonstrated that immune response-, calcium handling-, and mitochondrial metabolism-related genes were differentially expressed. In the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology pathway analyses, immune response pathways involved in cytokine interaction, nuclear factor-kappa B, and T-cell receptor signaling, calcium handling pathways, and mitochondria metabolism pathways involved in the citrate cycle were significantly upregulated. In protein interaction network analysis, chemokine family-, muscle contraction process-, and immune response-related genes were identified as hub genes with many interactions. In addition, mitochondrial pathways involved in calcium signaling, cardiac muscle contraction, tricarboxylic acid cycle, oxidation-reduction process, and calcium-mediated signaling were significantly stimulated in KEGG and Gene Ontology analyses. Our results provide a comprehensive understanding of the genome-wide transcriptional changes of HDM-induced AD-like symptoms and the indicated genes that could be used as AD clinical biomarkers.

Downlink Transmit Power Allocation in Soft Fractional Frequency Reuse Systems

Donghee Kim,Jae Young Ahn,Hojoon Kim 한국전자통신연구원 2011 ETRI Journal Vol.33 No.1

Downlink transmit power allocation schemes are proposed for soft fractional frequency reuse (FFR) in loose and tightly coordinated systems. The transmit powers are allocated so that the loss of spectral efficiency from the soft FFR is minimized, and the required cell edge user throughput is guaranteed. The effect of the soft FFR on spectral efficiency is evaluated depending on the power allocation schemes and the number of subbands. Results show that the loss of spectral efficiency from the soft FFR can be reduced by configuring an appropriate number of subbands in the loosely coordinated systems. In tightly coordinated systems, results show that the loss of spectral efficiency can be minimized regardless of the number of subbands due to its fast coordination.

Simultaneous Evaluation of Thermal and Non-Thermal Effects of High-Intensity Focused Ultrasound on a Tissue-Mimicking Phantom

Kim, Yong Tae,Ma, Donghee,Sim, Jai Kyoung,Kim, Se-Hwa Elsevier 2018 Ultrasound in medicine & biology Vol.44 No.8

<P><B>Abstract</B></P> <P>Physiologically relevant phantoms with high reliability are essential for extending the therapeutic applications of high-intensity therapeutic ultrasound. Here we describe a tissue-mimicking phantom capable of quantifying temperature changes and observing non-thermal phenomena by high-intensity therapeutic ultrasound. Using polydiacetylene liposomes, we fabricated agar-based polydiacetylene hydrogel phantoms (PHPs) that not only respond to temperature, but also have acoustic properties similar to those of human liver tissue. The color of PHPs changed from blue to red depending on the temperature in the range 40°C–70°C, where the red/blue ratio of PHP had a good linearity of 99.06% for the temperature changes. Furthermore, repeated high-intensity focused ultrasound led to histotripsy on the PHP with liquefied and damaged areas measuring 0.7 and 4.0 cm<SUP>2</SUP>, respectively, at the signal generator amplitude setting voltage of 80 mV. Our results indicate not only the usability of the thermochromic phantom, but also its potential for evaluating non-thermal phenomena in various high-intensity focused ultrasound therapies.</P>

Impact of T-cell-specific Smad4 deficiency on the development of autoimmune diabetes in NOD mice

Kim, Donghee,Lee, Song Mi,Jun, Hee-Sook Nature Publishing Group 2017 Immunology and Cell Biology Vol.95 No.3

<P>Type 1 diabetes results from autoimmune-mediated pancreatic beta-cell destruction and transforming growth factor-beta (TGF-β) is known to play a preventive role in type 1 diabetes in non-obese diabetic (NOD) mice. In this study, we investigated the role of Smad4, a key molecule for Smad-dependent TGF-β signaling, in T cells of NOD mice in the pathogenesis of autoimmune diabetes. We generated T-cell-specific Smad4 knockout (Smad4 tKO) NOD mice and assessed the pathological and immunological changes. Smad4 tKO showed earlier onset and increased incidence of diabetes than wild type (WT) NOD mice. Pathological features such as insulitis, anti-glutamic acid decarboxylase auto-antibody levels and serum IFN-γ levels were significantly increased in Smad4 tKO compared with WT NOD mice. Proportion and number of activated/memory CD4<SUP>+</SUP> T cell were significantly increased in pancreatic lymph nodes of Smad4 tKO compared with WT NOD mice. However, the proportion and function of regulatory T cells was not different. Effector CD4<SUP>+</SUP> T cells from Smad4 tKO were more resistant to suppression by regulatory T cells than effector cells from WT NOD mice. The proliferative potential of effector T cells from Smad4 tKO was significantly elevated compared with WT NOD mice, and activation of sterol regulatory element binding protein-1c (SREBP-1c) in T cells of Smad4 tKO NOD mice was correlated with this proliferative activity. We conclude that Smad4 deletion in T cells of NOD mice accelerated the development of autoimmune diabetes and increased the incidence of the disease by dysregulation of T cell activation at least in part via SREBP-1c activation.</P>

The lowest copy number of Porcine Endogenous retrovirus from Jeju Pigs

Donghee Lee,Jong-Kwang Yoon,Hoon-Mi Kim,Dieu Linh Tran,Young Bong Kim 한국동물생명공학회(구 한국동물번식학회) 2007 발생공학 국제심포지엄 및 학술대회 Vol.2007 No.1

Protective Effects of <i> Broussonetia kazinoki</i> Siebold Fruit Extract against Palmitate-Induced Lipotoxicity in Mesangial Cells

Kim, Donghee,Kim, Hyo-Jin,Cha, Seon-Heui,Jun, Hee-Sook Hindawi 2019 Evidence-based Complementary and Alternative Medic Vol.2019 No.-

<P>Diabetic nephropathy is one of the most serious complications of diabetes. Lipotoxicity in glomerular mesangial cells is associated with the progression of diabetic nephropathy. Paper mulberry,<I> Broussonetia kazinoki</I> Siebold (BK), has been used in oriental medicine for human health problems. However, to date, the beneficial effect of BK fruit has not been studied. In this study, we investigated the protective effect of an ethanolic extract of BK fruit (BKFE) against palmitate- (PA-) induced toxicity in mesangial cells. BKFE significantly increased the viability of PA-treated SV40 MES13 cells. BKFE significantly inhibited PA-induced apoptosis and decreased the expression of apoptotic genes, cleaved caspase-3, and cleaved PARP. Moreover, BKFE inhibited the expression of endoplasmic reticulum (ER) stress-related genes, such as BiP, phosphorylated eIF2<I>α</I>, cleaved ATF6, and spliced XBP-1, in PA-treated SV40 MES13 cells. BKFE decreased PA-induced ROS production. In addition, BKFE activated the transcription factor Nrf2 and increased the expression of antioxidant enzymes. However, knockdown of Nrf2 using siRNA suppressed this BKFE-induced increase in antioxidant enzyme expression. Furthermore, the protective effect of BKFE on PA-induced apoptosis was significantly reduced by Nrf2 knockdown. In conclusion, BKFE induced the expression of antioxidant enzymes via activation of Nrf2 and protected against PA-induced lipotoxicity in mesangial cells.</P>

Microscopic observation of frost behaviors at the early stage of frost formation on hydrophobic surfaces

Kim, Hisuk,Kim, Donghee,Jang, Hanmin,Kim, Dong Rip,Lee, Kwan-Soo Pergamon Press 2016 International journal of heat and mass transfer Vol. No.

<P><B>Abstract</B></P> <P>Microscopic seed behavior in the early stage of frost formation was experimentally observed with different fin surface contact angles from bare to superhydrophobicity under air-source heat pump operating conditions. The seed average height, radius, number, and frost density at the early stage of frost were analyzed. As the surface contact angle increased, the seed average height and number increased, while the seed radius and frost density decreased. A correlation with a Fourier number was proposed from the measured data. With the correlation, the large and small frost retardation effect regions were identified.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Frost formation in early stages is observed with different surface contact angles. </LI> <LI> The reasons of frost retardation with hydrophobic surface treatments are analyzed. </LI> <LI> Fourier number map for evaluating the effectiveness of the surface treatments is proposed. </LI> </UL> </P>

Solvent-Free Nanocomposite Colloidal Fluids with Highly Integrated and Tailored Functionalities: Rheological, Ionic Conduction, and Magneto-Optical Properties

Kim, Donghee,Kim, Younghoon,Cho, Jinhan American Chemical Society 2013 Chemistry of materials Vol.25 No.19

<P>We introduce a unique and facile strategy for the preparation of solvent-free nanocomposite colloidal fluids that allows accurate control over the integration of functionalities as well as the composition and dimensions of the nanocomposite structure. For the preparation of colloidal fluids with highly integrated functionalities, oleic acid (OA)-stabilized magnetic nanoparticles (i.e., OA-Fe<SUB>3</SUB>O<SUB>4</SUB> NPs) and CdSe@ZnS quantum dots (QDs) were first synthesized in nonpolar solvent. In this case, OA-QDs dispersed in toluene were successively phase transferred to thiol-functionalized imidazolium-type ionic liquid (IL-SH) media with rheological and ionic conduction properties. After the functional NPs were synthesized, amine-functionalized dendrimers and OA-Fe<SUB>3</SUB>O<SUB>4</SUB> NPs were alternately deposited onto silica colloids (i.e., SiO<SUB>2</SUB>/(dendrimer/OA-Fe<SUB>3</SUB>O<SUB>4</SUB>)<I><SUB>n</SUB></I>) using a ligand-exchange-induced LbL-assembly in organic media. Electrostatic LbL-assembled (anionic polyelectrolyte (PE)/cationic IL-SH-QD)<I><SUB>n</SUB></I> multilayers were then sequentially adsorbed onto the outermost dendrimer layer of the magnetic colloids. The resulting functional colloidal fluids were devoid of colloidal aggregation and exhibited strong superparamagnetic, fluorescent, rheological, and ionic conduction properties at room temperature. Furthermore, mixtures of photoluminescent colloidal fluids with and without OA-Fe<SUB>3</SUB>O<SUB>4</SUB> NPs behaved effectively as magneto-optically separable colloidal fluids. Because a variety of inorganic NPs ranging from metal to transition-metal oxides can be easily incorporated into colloidal substrates via LbL-assembly, our approach provides a basis for exploiting and designing functional colloidal fluids with liquidlike behavior at room temperature.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/cmatex/2013/cmatex.2013.25.issue-19/cm401560r/production/images/medium/cm-2013-01560r_0009.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/cm401560r'>ACS Electronic Supporting Info</A></P>

Characterization of the replication-competent porcine endogenous retrovirus class B molecular clone originated from Korean domestic pig

Kim, Na Young,Lee, Donghee,Lee, Jungeun,Park, Eung Woo,Jung, Woon-Won,Yang, Jai Myung,Kim, Young Bong Springer-Verlag 2009 Virus genes Vol.39 No.2