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Clonogenic Assay of Gastric Adenocarcinoma Stem Cells
Moon, hee Sook,Kim, Boo Sung,Kim, Hoon Kyo,Kim, Dong Jip,Lee, kyung Shik,Moon, Hanlim CATHOLIC MEDICAL CENTER 1986 Bulletin of the Clinical Research Institute Vol.14 No.1
The clonogenic assay means culturing tumor stem cells, which are responsible for tumor growth by self renewal and differentiation to tumor end cells. Elimination of the malignant stem cells or their self renewal will lead cure of tumor. Thus, their behavior of the stem cells by the clonogenic assay has been correlated with prognosis and outcome of therapy. We studied the clonogenic assay by'means of double agar system for 21 patients with advanced stomach cancer. The colony formation evaluated on 14th day of the culture was grown from 14 of 19 malignant effusions plated and 1 of 2 tumor nodules plated. The number of the colonies ranged from 5 to 96 per petri dish, and the median number was 20. The plating efficiency ranged from 0.001 to 0.036%, and the median was 0.003%. In the morphologic studies, the colonies made of tightly packed cells were grown from the poorly differentiated adenocarcinoma, whereas the colonies made of loosely packed cells with mucin formation were grown from the mucin secreting adenocarcinoma of stomach. The chemosensitivity in vitro tests to cisplatin were performed in 5 patients, and the results showed 4 sensitive and 1 resistant patients. The result of in vivo study with instillation of intraperitoneal cisplatin revealed that 3 patients "in vitro sensitive" were "responsive in vivo", and 1 patient "in vitro resistant" was "non-responsive in vivo". We conclude that the clonogenic assay of stomach cancer is useful method to understand the biology of the stem cell pool and select proper chemotherapy according to the chemosensitivity test.
Automation of Inventory Checking System for Outdoor Warehouse
Sung Moon Bae(배성문),Kwan Hee Han(한관희),Hwa Yong Lee(이화용),Kum Suk Hong(홍금석) 한국산업경영시스템학회 2018 한국산업경영시스템학회지 Vol.41 No.3
Inventory inspection is an important task to eliminate the inconsistency between real assets and inventory management systems, and it is performed periodically at the site. In the case of a large-sized property, it is mainly managed in an outdoor workshop or a warehouse. However, due to the large area of the outdoor area, it is relatively difficult to identify the quantity and location of the property and is also vulnerable to theft. To solve this problem, we proposed a method and system for performing a warehouse inventory inspection using an unmanned aerial vehicle (UAV) and Bluetooth low energy (BLE) beacons. The proposed method has the following advantages. First of all, if we carry out inventory inspection by utilizing UAV, we can minimize user’s effort compared to existing methods. The method of recognizing the asset by attaching the BLE beacon is more costly than using the existing RFID technology, but it is advantageous because the recognition distance is increased and the battery life of the tag is drastically increased. We also designed a BLE beacon reader for the system and implemented a prototype to show the feasibility of the proposed system. The prototype is based on Genuino 101, which is a kind of arduino, and adds HM-10 and Neo-6m modules to provide Bluetooth and GPS functions. The BLE beacon reader was tested in outdoor, and attached in drone. We also developed an inventory checking system based on the web to display results of inventory checking. The proposed approach enables the users to automate the operation of inventory checking on an outdoor warehouse.
Fabrication of Simulated DUPIC Fuel
Moon, Je Sun,Yang, Myung Seung,PARK, HEE SUNG,Kang, Kweon Ho,Song, Ki Chan 대한금속재료학회(대한금속학회) 2000 METALS AND MATERIALS International Vol.6 No.6
Simulated DUPIC fuel provides a convenient way to investigate the DUPIC fuel properties and behavior such as thermal conductivity, thermal expansion, fission gas release, leaching, and so on without the complications of handling radioactive materials. Several pellets simulating the composition and microstructure of DUPIC fuel are fabricated by resintering the powder, which was treated through OREOX process of simulated spent PWR fuel pellets, which had been prepared from a mixture of UO₂ and stable forms of constituent nuclides. The key issues for producing simulated pellets that replicate the phases and microstructure of irradiated fuel are to achieve a submicrometre dispersion during mixing and diffusional homogeneity during sintering. This study describes the powder treatment, OREOX, compaction and sintering to fabricate simulated DUPIC fuel using the simulated spent PWR fuel. The homogeneity of additives in the powder was observed after attrition milling. The microstructure of the simulated spent PWR fuel agrees well with the other studies. The leading structural features observed are as follows: rare earth and other oxides dissolved in the UO₂ matrix, small metallic precipitates distributed throughout the matrix, and a perovskite phase finely dispersed on grain boundaries.
Sang-Ho Jeon,Sunhwa Park,Da-Hee Song,Jong-yeon Hwang,Moon-su Kim,Hun-Je Jo,Deok-hyun Kim,Gyeong-Mi Lee,Ki-In Kim,Hye-Jin Kim,Tae-Seung Kim,Hyen-Mi Chung,Hyun-Koo Kim 한국토양비료학회 2017 한국토양비료학회지 Vol.50 No.5
To establish new metal groundwater standard, 5 metals such as aluminum, chromium, iron, manganese, and selenium were evaluated by Chemical Ranking Of groundWater pollutaNts (CROWN) including possibility of exposure, toxicity, interest factor, connection standard for other media, and data reliability. 430 groundwater samples in 2013 and 2014 were collected semiannually from 110 groundwater wells and they were analyzed for selenium, manganese, iron, chromium, and aluminum. For this study, 430 groundwater samples were categorized into 3 geological distribution features, such as igneous, metamorphic, and sedimentary rock region and geological background levels were divided by pre-selection methods. For the results, the average concentrations of aluminum, chromium, iron, manganese, and selenium in 430 groundwater samples were 0.0008 mg L<SUP>-1</SUP>, 0.0001 mg L<SUP>-1</SUP>, 0.174 mg L<SUP>-1</SUP>, 0.083 mg L<SUP>-1</SUP>, and 0.0004 mg L<SUP>-1</SUP><SUP></SUP>, respectively. In addition, among various geologies, average concentration of selenium was the highest in igneous rock region, average concentrations of chromium, manganese and aluminum were the greatest in sedimentary rock region, and average concentration of iron was the most high in metamorphic rock region. As a result of the geological background concentration with pre-selection method, background concentrations of selenium and aluminum in groundwater samples were the highest from sedimentary rock as 0.0010 mg L<SUP>-1</SUP> and 0.0029 mg L<SUP>-1</SUP> and background concentrations of manganese and iron in groundwater samples were the greatest from metamorphic rock as 0.460 mg L<SUP>-1</SUP> and 1.574 mg L<SUP>-1</SUP>, and no chromium background concentration in groundwater samples was found from all geology.