Stepwise Approach on Spent Nuclear Fuel Integrity Evaluation R&D Work in Korea

Donghak Kook,Jaeyong Kim,Yongsik Yang,Changwhan Shin 한국방사성폐기물학회 2017 한국방사성폐기물학회 학술논문요약집 Vol.2017 No.05

Human steroid sulfatase enhances aerobic glycolysis through induction of HIF1α and glycolytic enzymes

Shin, Sangyun,Kwon, Yeo-Jung,Ye, Dong-Jin,Baek, Hyoung-Seok,Kwon, Tae-Uk,Kim, Donghak,Chun, Young-Jin Elsevier/North Holland [etc.] 2019 Biochimica et biophysica acta Vol.1865 No.9

<P><B>Abstract</B></P> <P>Human steroid sulfatase (STS) has been linked with poor prognosis in steroid-associated tumors and represents an important clinical target in cancers, yet the mechanism of STS-induced carcinogenesis remains unclear. To correlate STS with cancer metabolism, we determined the effects of STS on aerobic glycolysis. STS overexpression increased cellular levels of lactic acid, the final product of aerobic glycolysis. Moreover, STS suppressed the oxygen consumption rate (OCR), which represents mitochondrial respiration. Inhibition of STS by the specific inhibitor STX064 recovered STS-induced OCR repression and lactic acid over-production. DHEA, but not DHEA-S, suppressed the OCR level and enhanced lactic acid production. To understand the molecular mechanism of STS-induced cancer metabolism, we measured the expression of glycolytic enzymes hexokinase 2 (HK2) and pyruvate kinase M2 (PKM2), which was highly upregulated by STS and DHEA at both protein and mRNA levels. HIF1α is a key mediator of aerobic glycolysis, and STS enhanced HIF1α promoter activity, mRNA expression, and protein expression. Down-regulation of HIF1α by siRNA suppressed the HK2 and PKM2 expression induced by both STS and DHEA. HIF1α siRNA also recovered the OCR repression and lactic acid over-production induced by both STS and DHEA. To explore the mechanism <I>in vivo</I>, we produced transgenic mice overexpressing STS and found that STS expression was particularly enhanced in the lung. Consistent with our <I>in vitro</I> results, the expression of HIF1α, HK2, and PKM2 was also increased in mouse lung tissues. In conclusion, we suggest that STS may induce aerobic glycolysis through enhancing HIF1α expression.</P> <P><B>Highlights</B></P> <P> <UL> <LI> STS and its metabolite DHEA increase lactic acid production and suppress oxygen consumption in HeLa cells. </LI> <LI> STS induces expression of glycolytic enzymes both <I>in vivo</I> and <I>in vitro</I>. </LI> <LI> STS and DHEA triggers HIF1α induction. </LI> <LI> HIF1α is a crucial mediator of STS-induced aerobic glycolysis. </LI> </UL> </P>

Improved depth extraction method of 3D objects using computational integral imaging reconstruction based on multiple windowing techniques

Yoo, Hoon,Shin, Donghak,Cho, Myungjin Elsevier 2015 Optics and lasers in engineering Vol.66 No.-

<P><B>Abstract</B></P> <P>This paper presents an improved depth extraction method of 3D objects using computational integral imaging reconstruction (<I>CIIR</I>) based on the multiple windowing models. The proposed method records 3D objects using the lenslet array; and it reconstructs multiple sets of slice images from multiple <I>CIIR</I> methods based on the different windowing models. A depth map is then extracted by a block matching algorithm among multiple set of slice images. A preliminary experiment is carried out to show the feasibility of the proposed method. Experimental results indicate the proposed method outperforms the previous method with two windowing models.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We present an improved depth extraction method of 3D objects using computational integral imaging reconstruction. </LI> <LI> The proposed method is based on the multiple windowing models. </LI> <LI> We introduce a new depth extraction algorithm using block matching among multiple set of slice images. </LI> <LI> The proposed method can extract the improved depth map through the reduction of unwanted depth errors. </LI> </UL> </P>

Viewing Angle-Improved 3D Integral Imaging Display with Eye Tracking Sensor

Hong, Seokmin,Shin, Donghak,Lee, Joon-Jae,Lee, Byung-Gook The Korea Institute of Information and Commucation 2014 Journal of information and communication convergen Vol.12 No.4

In this paper, in order to solve the problems of a narrow viewing angle and the flip effect in a three-dimensional (3D) integral imaging display, we propose an improved system by using an eye tracking method based on the Kinect sensor. In the proposed method, we introduce two types of calibration processes. First process is to perform the calibration between two cameras within Kinect sensor to collect specific 3D information. Second process is to use a space calibration for the coordinate conversion between the Kinect sensor and the coordinate system of the display panel. Our calibration processes can provide the improved performance of estimation for 3D position of the observer's eyes and generate elemental images in real-time speed based on the estimated position. To show the usefulness of the proposed method, we implement an integral imaging display system using the eye tracking process based on our calibration processes and carry out the preliminary experiments by measuring the viewing angle and flipping effect for the reconstructed 3D images. The experimental results reveal that the proposed method extended the viewing angles and removed the flipping images compared with the conventional system.

미세유체소자와 디지털 홀로그래피 기술을 이용한 미생물의 3D 이미징과 세그먼테이션

신동학,이준재,Shin, Donghak,Lee, Joon-Jae 한국정보통신학회 2013 한국정보통신학회논문지 Vol.17 No.2

미세유체소자(microfluidic device)는 미생물과 관련된 다양한 작업들에 대해서 정확한 제어를 제공할 수 있다. 본 논문에서는 미세유체 소자와 디지털 홀로그래피 마이크로스코피 기술로 구성된 시스템을 구성하고 미생물의 3D 이미징과 세그먼테이션을 설명한다. 각각의 미생물은 미세유체 채널을 통하여 흘러가며 홀로그래피 마이크로스코피가 홀로그램을 기록한다. 기록된 홀로그램은 Fresnel 변환을 통하여 컴퓨터적으로 복원되며, 복원된 영상의 위상성분을 이용하여 미생물의 위치 정보를 찾기 위한 세그먼테이션을 수행한다. 제안하는 방법의 유용함을 설명하기 위하여 광학 실험을 수행하고 그 결과를 나타내었다. Microfluidic devices can offer precise control for a verity of tasks involving biological specimen. In this paper, we propose an integrated system consisting of a microfluidic device along with a digital holographic microscope and present three-dimensional (3D) sensing and segmentation of biological microorganisms. When the individual microorganisms are inputted into the microfluidic channel, the holographic microscope records their holograms. The holograms are computationally reconstructed in 3D using Fresnel transform and the reconstructed phase images are used to search the position of microorganisms. Optical experiments are carried out and experimental results are presented to illustrate the usefulness of the proposed system.

Towards 3D Television Through Fusion of Kinect and Integral-Imaging Concepts

Seokmin Hong,Donghak Shin,Byung-Gook Lee,Dorado, Adrian,Saavedra, Genaro,Martinez-Corral, Manuel IEEE 2015 Journal of display technology Vol.11 No.11

<P>We report a new procedure for the capture and processing of light proceeding from 3D scenes of some cubic meters in size. Specifically we demonstrate that with the information provided by a kinect device it is possible to generate an array of microimages ready for their projection onto an integral-imaging monitor. We illustrate our proposal with some imaging experiment in which the final result are 3D images displayed with full parallax.</P>

Resolution-enhanced integral imaging in focal mode with a time-multiplexed electrical mask array.

Oh, Yongseok,Shin, Donghak,Lee, Byung-Gook,Jeong, Shin-Il,Choi, Hee-Jin Optical Society of America 2014 Optics express Vol.22 No.15

<P>The inferior resolution of the three-dimensional (3D) image is one of the main problems to be resolved for realizing a commercial autosteresosopic 3D display device. In this paper, a time-multiplexing technique using electrically moving masks is proposed to enhance the resolution of the 3D image realized by integral imaging in a focal mode while preserving the viewing angle of it.</P>

향상된 물체 인식을 위한 픽셀 복원 기반의 비선형 3D 상관기

신동학,이준재,Shin, Donghak,Lee, Joon-Jae 한국정보통신학회 2013 한국정보통신학회논문지 Vol.17 No.3

본 논문에서는 향상된 물체 인식을 위한 픽셀 복원 기반의 비선형 3D 상관기를 제안한다. 제안한 방법은 부분적으로 가려진 물체로부터 요소영상을 픽업하고 서브영상으로 변환하고 영역 매칭 알고리즘 방법을 이용하여 서브영상으로부터 장애물로 가려진 영역을 검출하고 제거한다. 그 다음 픽셀 복원 방법으로 각 서브영상에서 제거된 물체의 픽셀을 복원한다. 마지막으로, 재생된 참조영상과 재생된 영상 사이의 비선형 상호상관을 통하여 3D 물체의 인식 성능을 향상 시킨다. 제안된 방법의 유용함을 보이기 위해 기존 방법과 비교하여 기초적인 상관관계 실험을 수행하고 그 결과를 보고한다. In this paper, we propose a performance-enhanced object recognition by using nonlinear 3D correlator based on pixel restoration. In the proposed method, elemental images of the 3D target that are partially occluded by a foreground object are picked up and transformed into sub-images. By using the block-matching algorithm, the occluded target regions of each sub-image are estimated and removed. After that, the missing pixels in each sub-image are reestablished by using the pixel-restoration method. Finally, through the nonlinear cross-correlations between the reconstructed reference and the target plane images, the improved object recognition can be performed. To show the feasibility of the proposed method, some preliminary experiments are carried out and results are presented by comparing the conventional method.

고속 컴퓨터 집적 영상 복원 방법을 이용한 비선형 3D 영상 상관기

신동학,이준재,Shin, Donghak,Lee, Joon-Jae 한국정보통신학회 2012 한국정보통신학회논문지 Vol.16 No.10

본 논문에서는 고속 컴퓨터 집적 영상 복원 방법을 이용하여 새로운 형태의 3D 비선형 상관기를 제안한다. 고속 컴퓨터 집적 영상 복원 방법을 구현하기 위해서 기존의 방법에서 확대 과정을 제거함으로서 고속 계산이 가능하다. 제안하는 상관기는 먼저 기준 물체와 목표 물체의 요소 영상들을 렌즈 배열을 통해 픽업한다. 이 픽업된 영상에 고속 컴퓨터 집적 영상 복원 방법을 사용하여 목표 평면 영상과 기준 평면 영상들이 복원된다. 복원된 기준 평면 영상과 목표 평면 영상들 간의 비선형 상호상관을 통해 인식을 수행한다. 비선형 상관 연산의 사용은 상관기의 3D 물체 인식 성능 향상시킬 수 있다. 제안된 방법의 유용함을 보이기 위해 기존의 방법과 비교하여 기초적인 상관관계 실험을 수행하고 그 결과를 보고한다. In this paper, we propose a novel nonlinear 3D image correlator using a fast computational integral imaging reconstruction (CIIR) method. In order to implement the fast CIIR method, the magnification process was eliminated. In the proposed correlator, elemental images of the reference and target objects are picked up by lenslet arrays. Using these elemental images, reference and target plane images are reconstructed on the output plane by means of the proposed fast CIIR method. Then, through nonlinear cross-correlations between the reconstructed reference and the target plane images, the pattern recognition can be performed from the correlation outputs. Nonlinear correlation operation can improve the recognition of 3D objects. To show the feasibility of the proposed method, some preliminary experiments are carried out and the results are presented by comparing the conventional method.

학동전기 소아의 체중과 신체활동도와의 관계

서영성,김대현,신동학 대한비만학회 2000 The Korean journal of obesity Vol.9 No.2

유아에서 신체활동도 및 생활 습관이 유아의 체중에 미치는 영향에 대하여 알아보고자 1997년 9월 대구시내 소재하는 모 어린이집 원생 113명 (만 3 ~ 6세 유아) 을 대상으로 걸음 수를 기록하는 만보계를 이용하여 신체활동도를 측정하였고, 비만도의 판정은 키에 따른 표준체중에 현재체중과 비교하는 방법으로 비만도를 추정하였고, 비만도의 판정은 키에 따른 표준체중에 현재체중과 비교하는 방법으로 비만도를 추정하였고, 유아의 부모에게 설문지를 보내어 조부모 동거, 형제유무, 부모운동유무 등의 가정환경을 조사하였고, 생활습관은 총 수면시간, TV 및 비디오 시청시간, 과외활동 유무로 조사하였으며, 식사습관은 아침식사 유무, 간식유무, 음식남김여부, 식욕유무 등을 조사하여 다음과 같은 결과를 얻었다. 1. 남아는 58명 (51.3%) 여아는 55명 (48.7%)이었고 평균 나이는 남아 4.4±0.72세 여아는 4.6±0.99세였다. 표준체중표에 의한 비만도 평균은 남아 6.4±8.60%였고 여아는 7.5±11.73%였다. 표준체중의 20% 이상을 비만으로 판정했을 때 비만한 유아는 6.2% (7명)였다. 2. 만보계로 측정된 신체활동도는 남아 2088.9±794.81보, 여아 2012.0±701.37보 였다. 나이, 비만도, 신체활동도에서 남녀 소아간의 유의한 차이가 없었다. 3. 식사습관과 비만도의 비교에서 식사시 음식을 남기지 않는 유아가 남기는 유아에 비해 유의하게 비만도가 높았으나 (P=0.008), 아침식사유무, 간식유무와 비만도의 비교에서는 유의한 차이가 없었다. 4. 비만도와 생활습관 및 식사습관간의 상관분석에서 비만도가 클수록 신체활동도는 유의하게 적었고 (P<0.01), 음식을 남기지 않았으며 (P<0.05), 편식하지 않았으며 (P<0.05), TV/Video 시청시간이 많았다 (p<0.05). 비만도와 총수면시간, 과외활동유무, 간식유무 및 아침 식사유무, 저녁식사 시간과는 무관하였다. 유아의 체중은 신체활동도와 밀접한 관련이 있었고 식습관도 관련이 많으므로, 유아의 체중과 생활 습관이 소아 및 성인으로 이어진다고 가정할 때 일차진료의사들은 유아를 둔 부모들에게 유아의 신체활동을 증가시킴과 동시에 건전한 식습관을 가지도록 교육하여야 한다고 사료되어진다. The objective of this study was to assess the influence of physical activity on body weight in preschool children. The 3 ~ 6 year-old physically healthy 113 early childhood in one children day care house were selected for this study. body weight, height and physical activity were measured. Measurement of physical activity was walking number by accelerometer (electronic motion sensor, manbogi) during day care house (for 6 hours). Other daily living behavior data for subjects were gathered by parent's questionnaire. Subjects with overweight for age were 35.4%, obesity with early childhood were 6.2%. Walking number for overweight subjects by accelerometer were less than other preschool children's that (P=0.013). Also, subjects with overweight were correlated with eating behavior, especially more left over of food and favorite food intake than normal weight subjects. Early childhood with overweight had more TV/video viewing time than standard weight subject's that. But sleeping time, snack frequency, breakfast, dinner time were not correlated with body weight. We suspect early childhood body weight is closely correlated with daily physical activity, eating behavior, and TV/Video viewing time. We recommend primary care physicians may be educate to parent with overweight preschool children about physical activity, eating behavior and daily living habits.