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이유철,배혜림 한국정밀공학회 2012 International Journal of Precision Engineering and Vol. No.
The set of activities intended to ensure their products which can be goods and/or services, is getting crucial to the successful business in almost competitive markets. Though the mass production fields like electronics and automotive industries have matured and versatile methodologies on quality issues, in the case of small quantity batch processes especially after various manufacturing processes, problems of quality assurance highly depends on determination by the intended users, clients or customers. This study suggests digital simulation processes to cover wide categories of products required expensive real experiments under specified hydro-pressures. To cope with the diverse changes after manufacturing from nominal thickness of raw pipes, all geometry modeling reflected measured thickness distributions over specified points and validation tests for numerical simulations were performed to minimize the number of product disposals as the process of design proof tests. Through this project, the manufacturer established a business process of numerical simulation and validations, which make it possible that they are very confident from early stage of bidding to service steps after deliveries in terms of not only technical power but overall quality assurance, because they successfully attained meaningful agreements with major clients by proposed digital simulation process in this paper.
이유철,설성용,조동택,전도기,Lee, Yoo-Chul,Seol, Sung-Yong,Cho, Dong-Taek,Chun, Do-Ki 대한미생물학회 1987 大韓微生物學會誌 Vol.22 No.1
Multiply resistant Shigella strains isolated in Taegu area were subjected for the characterization of R plasmids. All strains isolated in 1984 and 1985 were susceptible to gentamicin, amikacin, and cephalothin, and most strains were susceptible to kanamycin (Km) and rifampin by agar dilution antimicrobial susceptibility test. The resistance frequency of S. flexneri against ampicillin (Ap) was higher than that of S. sonnei. The strains resistant to sulfisomidine (Su) and trimethoprim (Tp) were found at higher frequency in S. sonnei than in S. flexneri. The most prevalent resistance pattern of S. flexneri was chloramphenicol (Cm) tetracycline (Tc) streptomycin (Sm) Ap, followed by the pattern of CmTcSmSuApTp, CmTcSmSuApTp nalidixic acid, and CmTcSmSuAp in the decreasing order. The antibiogram of CmTcSmSuTp was found to be the most frequent pattern in S. sonnei. The ratio of conjugal transfer of S. flexneri was 47% and 75% of S. sonnei. The average number of plasmid harboring in Shigella was 4 and the size of plasmid ranged 1.3 to 134 megadalton (Mdal). Most S. flexneri carried plasmids of 2 to 3 Mdal and S. sonnei carried those of 3 to 4 Mdal size. The sizes of conjugative plasmids ranged 40-90 Mdal. The incompatibility group (Inc) F II plasmids (54-59 Mdal) were most frequent and rare Inc B plasmids (60 Mdal) of isolates in 1979 and 1980 and Inc FI (87 Mdal) of 1983 isolates were able to be classified by the colony test with standard reference plasmids. The R plasmids of known Inc group were tested for the restriction endonuclease analysis. The pattern of plasmids digested by EcoRl were apparently different by the Inc group but there was no significant difference between species or by the resistance patterns. Nonconjugative plasmids and their phenotypes were identified by transformation test. The transformants were resistant to less than two drugs. Colicin producing transformants carried the Col plasmid of 3.7 or 3.9 Mdal size. $Ap^r$ plasmids derived from S. sonnei were found to be mobilized by transfer factor RT641 to E. coli #CS100. $Ap^r$ plasm ids of same size shared by S. flexneri, S. sonnei, and E. coli were digested with Pstl. All of them showed two restriction fragments of 2.8 kilobase(kb) and 0.7kb. Other plasmids ($Sm^r\;Su^r$) derived from S. flexneri, S. boydii, and S. sonnei were digested with Pstl and they showed same restriction fragment patterns of 3.1kb and 2.9kb. The plasmid profiles of three strains of S. sonnei producing colicin and showing same resistance pattern of CmTcSmSuApTpKm appeared to be similar. Restriction patterns by EcoRl and the behavior of plasmids in conjugation or transformation process were also similar between those plasmids. The restriction patterns were significantly different between the plasmids of Inc FI group and those of unclassified Inc group.
A revision of the genus Littorina (Mollusca: Gastropoda) in Korea
이유철,최영재,Elizabeth M. A. Kern,신영헌,김태호,박중기 한국통합생물학회 2018 Animal cells and systems Vol.22 No.6
Littorina Férussac, 1822 is an abundant genus of small gastropods found in the upper littoral zone of rocky seashores worldwide. Although ecologically important, shell-based species identification in this genus is challenging due to phenotypic variation in shell morphology and lack of diagnostic characters among morphologically similar species. In this study, we revised the taxonomy of Korean Littorina species using morphological characters (shell and radula) and cox1 mitochondrial DNA sequences for three Korean species: L. brevicula, L. sitkana, and L. horikawai. Results suggest that L. sitkana was erroneously reported as L. kasatka in a previous study. A new record for Littorina horikawai (Matsubayashi & Habe in Habe, 1979), previously unknown from Korea, is described, which can be distinguished from L. sitkana by the presence of alternating white and brown spiral ribs on each whorl. Comparison of the mtDNA cox1 gene sequences shows very low intraspecific variation even between geographically distant populations. A phylogenetic tree supports a close relationship between L. horikawai and L. sitkana, consistent with earlier phylogenetic studies.
자동차 산업분야의 효과적인 제조협업 구현을 위한 디지털 엔지니어링 적용 방법론에 대한 연구
이유철,배혜림 대한산업공학회 2012 산업공학 Vol.25 No.1
Very special and tentative considerations including emotional aspects are required to apply any new mechanism and methodologies for manufacturing fields due to several reasons. This study reviews the characteristics of manufacturing collaborations through specific cases applied digital engineering to enhance the collaboration performance in manufacturing domains. Two cases of collaboration related with automotive manufacturing process are analyzed to extract meaningful insights for better collaboration model suggestions. The first case deals the robot simulation to find out advance errors in jig and fixture design during the various welding process of body-in-whites. The effective communication protocol to share their idea and agreed schedules are essential for this collaboration. More severe requirement of collaboration between R&D and manufacturing departments are studied in the second case for e-coating process. The invisible barriers among different departments are lowered by the process application of Computer Aided Engineering which can make sure their own interesting effectively. Those technical and managerial suggestions can be used when the information system and standard process are sought to implement and update not only when innovation projects are executed.