CONTROL SYSTEM OF THREE DIMENSIONAL HYDRAULIC SIMULATIVE ROCKING TEST-BED

Jiang, Chong Li,Ye, Wei Dong,Ming, Dong,Zhou, Hai Yang,Wang, Xiao Hui 대한전자공학회 1992 HICEC:Harbin International Conference on Electroni Vol.1 No.1

Three dimensional hydraulic simulative rocking test-bed is an experiment tool. It is drived by three dimensional hydraulic pressure, it could be applied to simulate various decline and rocking conditions to actual marine on sea as true as possible. It is vital test means to the equipments on the marine which need examination to decline and rocking condition. It is high accurate, safe, and convenient. By computer control, we strenghen the mobility of system, increase accuracy of trace, widen using scope. In this paper, we introduce sinuous servo system controlled by computer, system design, composition of system, stability analysis, program design, data processing and screen display.

Influence of Kluyveromyces marxianus on proteins, peptides, and amino acids in Lactobacillus-fermented milk

Dong-Dong Zhang,Jing-Lan Liu,Tie-Min Jiang,Lu Li,Guo-Zhen Fang,Yan-Pin Liu,Li-Jun Chen 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.3

With increasing application of yeast in fermented milk, in order to study the effect of yeast on milk protein during the fermentation process, the effects of the presence of Kluyveromyces marxianus in milk fermented by Streptococcus thermophilus and Lactobacillus bulgaricus were investigated. After fermentation, the amino acid, protein, and peptide contents were analyzed by ultra-performance liquid chromatography, two-dimensional gel electrophoresis, and liquid chromatography–mass spectrometry, respectively. After the addition of K. marxianus for fermentation, 25 protein spots changed significantly. These were mostly caseins and bovine serum proteins, and the content of total free amino acids increased by 16.30%; ten types of bioactive peptides were identified. Furthermore, the number of peptide types in milk fermented by K. marxianus increased significantly compared with milk fermented by Lactobacillus. K. marxianus is considered to promote proteometabolism in milk when added with Lactobacillus, generate flavor compounds, and improve the digestion and absorption character of milk.

Identifying a Cinnamoyl Coenzyme A Reductase (CCR) Activity with 4-Coumaric Acid: Coenzyme A Ligase (4CL) Reaction Products in Populus tomentosa

Dong-Dong Wang,Hua Bai,Wei-Qi Chen,Hai Lu,Xiang-Ning Jiang 한국식물학회 2009 Journal of Plant Biology Vol.52 No.5

A cinnamoyl coenzyme A reductase (CCR, EC 1.2.1.44), one of the key enzyme involved in lignin biosynthesis, was cloned from Populus tomentosa (Chinese white poplar). At the same time, a 4CL1 gene was cloned from P. tomentosa, too. The two genes were subcloned in pQE31 vector and expressed in Escherichia coli M15. Both of them were purified by Ni-NTA. Purified CCR protein was digested by trypsin and analyzed by HPLC-MS; the peptide segments had 27% similarity with the sequence of the CCR protein. 4CL was thought to be a neighbor enzyme of CCR in lignin biosynthesis. In this paper, a 4CL1 from P. tomentosa was cloned, and its enzyme reaction products were extracted for the substrates of CCR. Three 4CL1 enzyme reaction products were monitored by HPLC-MS and then the CCR enzyme reaction was detected by GC-MS. In the CCR reaction, the three corresponding aldehyde (p-coumaraldehyde, caffealdehyde, and coniferaldehyde) were detected and identified by Frontier3 software. The results showed that the CCR that we cloned from P. tomentosa had affinities with 4CL1 enzyme reaction products and a ptCCR that was cloned from aspen (Li et al., Plant Cell Physiol 46(7):1073–1082, 2005) only had affinity with feruloyl-CoA. The different results maybe depend on the different study method. The method of exacting 4CL enzyme products as the substrates of CCR in the paper was reliable and can be used in lignin biosynthesis network to detect the enzymes in the neighborhood that depended on the polarity of the substrates and products. This CCR gene had eight homology sequence CCR gene when a BLAST was conducted in Populus trichocarpa genome database. The CCR homology genes in Populus suggested that some CCRs may take part in the lignin biosynthesis, too. The gene family would be the hot spot in the future study.

Video Seamless Splicing Method Based on SURF Algorithm and Harris Corner Points Detection

Dong Jing,Chen Dong,Jiang Shuwen 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.4

Chinese Ballet during the Great Cultural Revolution

Jiang Dong 한국무용기록학회 2007 무용역사기록학 Vol.13 No.-

중국에서는 1966년부터 1976년까지 약 10년에 걸쳐 문화대혁명이 진행되었고 그 시기동안 모든 문화예술 작품은 8개의 ‘혁명 모범극(Revolutionary Model Plays)’으로 통합되어 공연되었다. 이 중 두 개의 작품이 발레였으며, 하나는 베이징에서 초연된 <붉은 여군(The Red Detachment of Women)>이고 다른 하나는 상하이에서 초연된 <백모녀(The White-haired Girl)>였다. 이들 발레 작품은 문화대혁명이 시작된 1966년 이전에 초연되었으나 문화대혁명 기간 동안 수정 과정을 거치면서 중국 인민들을 교육하는 ‘혁명 모범극’으로 재탄생하게 되었다. 문화대혁명 기간 중의 예술 창작이란 계층 간의 갈등 표출을 바탕으로 옛 것을 부정하고 새로운 혁명적 사상을 선전하는 또 다른 정치적 활동이었다. 당시 중국 혁명의 지도자인 마오쩌둥의 부인 장칭은 <붉은 여군>과 <백모녀> 두 발레 작품의 수정 과정에 직접 참여하였으며, 이는 결국 두 발레 작품이 정치적인 ‘모범극’으로서 재탄생하게 되는 계기가 되었다. 본 논문은 문화대혁명 기간 동안 진행된 두 발레 작품의 변화를 살펴보고 당시 매체들에 보도된 기사 등을 통해 장칭이 독재적으로 주도한 작품수정 과정 및 이를 통한 작품의 경직화 현상 등을 살펴보고, 나아가 이들이 중국의 무용 역사상 갖는 의의 등을 살펴보았다. In China, about for 10 years from 1966 until 1976, the Great Cultural Revolution went on. During that time, all artistic works were integrated as 8 Revolutionary Model Plays and performed. Of them, two works were ballets. One is The Red Detachment of Women, the first performance in Beijing and another is The White-haired Girl, the first performance in Shanghai. These ballet works had been premiered beforet the Great Cultural Revolution was started in 1966 but through several revision during the Great Cultural Revolution, they were recreated as 'Revolutionary Model Plays' for the purpose to educate Chinese people. Art creation during the Great Cultural Revolution, it was another political activity for negating the old things and instigating the revolutionary ideology, based on the class conflicts. At that time, Chiang Ching the wife of Mao Ze-dong, the leader of the Chinese Revolution, actively involved in revision of two ballet works, The Red Detachment of Women and The White-haired Girl. So it served as a momentum for the rebirth of two ballet works, changed into political "model plays". In this paper, it explored the change of two ballet works during the Great Cultural Revolution. And through the news aticles which were reported in mass media at that time, it investigated how Chiang Ching took a initiative participation in revision dictorially and how the dance works became rigid gradually, and furthermore this paper tried to examine the significance meaning in History of Dance in China.

Endostar Combined with Cisplatin Inhibits Tumor Growth and Lymphatic Metastasis of Lewis Lung Carcinoma Xenografts in Mice

Dong, Xiao-Peng,Xiao, Tian-Hui,Dong, Hong,Jiang, Ning,Zhao, Xiao-Gang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Objective: To investigate the effects of endostar, a recombined humanized endostatin, plus cisplatin on the growth, lymphangiogenesis and lymphatic metastasis of the Lewis lung carcinoma (LLC) in mice. Methods: A tumor model were established in C57BL/6 mice by intravenious transplantation of LLC cells. Then the mice were randomized to receive administration with NS, endostar, cisplatin, or endostar plus cisplatin. After the mice were sacrificed, tumor multiplicity, tumor size and lymph node metastasis were assessed. Then the expression of vascular endothelial growth factor-c (VEGF-C) and podoplanin were determined by immunohistochemical staining. Results: Endostar plus cisplatin significantly suppressed tumor growth. lymphatic metastasis and prolonged survival time of the mice without obvious toxicity. The inhibition of lymphatic metastasis was associated with decreased microlymphatic vessel density (MLVD) and expression of VEGF-C. Conclusions: Endostar combined with cisplatin was more effective to suppress tumor growth and lymphatic metastasis than either agent alone. Thus this may provide a rational alternative for lung carcinoma treatment.

Correlation between Meat Color and L-Carnitine Content in Livestock Meats

Jiang Ping Fan,Dong Yeop Kim,Gi Dong Han 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.1

In this study, the correlation between color of redness and L-carnitine content in meats was investigated using microplate enzymatic assays. The L-carnitine levels and its storage stabilities of domestic and imported livestock products in Korean markets were also studied. The results showed a high correlation (r=0.9764) between L-carnitine content and redness values of homogenized meat solution. Korean native cattle (‘Hanwoo’) meat showed the highest L-carnitine content (3.64±0.14 μ㏖/g) in meat samples analyzed in this study. The L-carnitine level of the meats decreases during periods of storage in cold and freezing conditions, and the level of decrease was more significant at 4℃ than at ?20℃, which suggests that the storage stability of L-carnitine is related to its storage temperature. This study gives reliable data about correlation between meat color of redness and L-carnitine content, and gives useful information to determine the characteristics of ‘Hanwoo’.

A New ent-Kaurane type Diterpenoid Glycoside from Inula japonica Thunb

Jiang Jiang Qin,Jia Xian Zhu,Wei Dong Zhang1,2,Yan Zhu,Jian Jun Fu,Xiao Hua Liu,Hui Zi Jin 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.10

A new ent-kaurane type diterpenoid glycoside, 17-O-β-D-glucopyranosyl-16α-ent-kauran-19-oic acid (1), together with 17-hydroxy-16α-ent-kauran-19-oic acid (2), 16α,17-dihydroxyl-ent-kauran-19-oic acid (3), and 16α-hydroxy-17-acetoxy-ent- kauran-19-oic acid (4) were isolated from the aerial parts of Inula japonica Thunb. The structure of 1 was determined mainly by use of 1D and 2D NMR spectroscopic techniques including HSQC, 1H-1H COSY, HMBC, and NOESY. In addition, 4 exhibited significant inhibitory activity on NO production in LPS-stimulated RAW264.7 cells with IC50 value of 14.3 μg/mL.

Rapid, Sensitive, and Specific Detection of Clostridium tetani by Loop-Mediated Isothermal Amplification Assay

( Dong Neng Jiang ),( Xiao Yun Pu ),( Jie Hong Wu ),( Meng Li ),( Ping Liu ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.1

Tetanus is a specific infectious disease, which is often associated with catastrophic events such as earthquakes, traumas, and war wounds. The obligate anaerobe Clostridium tetani is the pathogen that causes tetanus. Once the infection of tetanus progresses to an advanced stage within the wounds of limbs, the rates of amputation and mortality increase manifold. Therefore, it is necessary to devise a rapid and sensitive point-of-care detection method for C. tetani so as to ensure an early diagnosis and clinical treatment of tetanus. In this study, we developed a detection method for C. tetani using loop-mediated isothermal amplification (LAMP) assay, wherein the C. tetani tetanus toxin gene was used as the target gene. The method was highly specific and sensitive, with a detection limit of 10 colony forming units (CFU)/ml, and allowed quantitative analysis. While detecting C. tetani in clinical samples, it was found that the LAMP results completely agreed with those of the traditional API 20A anaerobic bacteria identification test. As compared with the traditional API test and PCR assay, LAMP detection of C. tetani is simple and rapid, and the results can be identified through naked-eye observation. Therefore, it is an ideal and rapid point-of-care testing method for tetanus.

C-type natriuretic peptide attenuates renal osteodystrophy through inhibition of FGF-23/MAPK signaling

Dong Dong Zhang,Yang Fang Wu,Wei Xia Chen,Yao Xu,Si Yan Liu,Huang Huang Luo,Guang Mei Jiang,Yue Wu,Peng Hu 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Renal osteodystrophy (ROD) occurs as early as chronic kidney disease (CKD) stage 2 and seems ubiquitous in almost all pediatric patients with CKD stage 5. Fibroblast growth factor (FGF)-23, a bone-derived endocrine regulator of phosphate homeostasis, is overexpressed in CKD and disturbs osteoblast differentiation and matrix mineralization. In contrast, C-type natriuretic peptide (CNP) acts as a potent positive regulator of bone growth. In the present study, we infused CNP into uremic rats and observed whether CNP could attenuate ROD through the inhibition of FGF-23 cascades. In uremic rats, CNP administration significantly alleviated renal dysfunction, calcium phosphate metabolic disorders, hypovitaminosis D, secondary hyperparathyroidism, the decrease in bone turnover markers and retarded bone pathological progression. More importantly, within FGF-23/mitogen-activated protein kinase (MAPK) signaling, the fibroblast growth factor receptor-1, Klotho and alternative (STAT-1/phospho-STAT-1) elements were upregulated by CNP, whereas FGF-23, RAF-1/phospho-RAF-1, and downstream (ERK/phospho-ERK and P38/phospho-P38) elements were paradoxically underexpressed in bone tissue. Therefore, CNP exerts a therapeutic effect on ROD through inhibition of FGF-23/MAPK signaling at the RAF-1 level.