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Qian Wu,Hui Wang,Juanjuan Wu,Dagang Wang,Yongli Wang,Lei Zhang,Zhiping Huang,Deyue Yu 한국식물학회 2015 Journal of Plant Biology Vol.58 No.4
The evaluation and use of endogenous soybean genes is an effective strategy to minimize the yield losses caused by insects. Allene oxide cyclase (AOC) catalyzes the most important step in the biosynthesis of jasmonate (JA), which plays a crucial role in plant defense against insects. In this study, the role of GmAOC3 in plant insect resistance was evaluated. Real-time PCR results indicate that GmAOC3 was uniquely and rapidly activated and attained peak expression in leaves after attack by the common cutworm (CCW). In insect bioassays, transgenic lines overexpressing GmAOC3 were significantly less damaged than wild-type plants, and the relative growth rate of CCW fed with leaves from transgenic lines was significantly lower than that of CCW fed with leaves from wild-type plants. Electron microscopy revealed that the density of leaf trichomes in transgenic lines overexpressing GmAOC3 was greater than that in wild-type tobacco. Several physiological and morphological indicators, including JA, phenolic content and the relative expression levels of the putrescine N-methyltransferase (PMT) and proteinase inhibitor (PI) genes, phenylalanine ammonia lyase (PAL) activity and volatile substances, increased in the transgenic plants overexpressing GmAOC3. Our findings indicate that GmAOC3 plays an important role in soybean resistance to CCW and can be used as a resource for plant breeding.
Deyu Xu,Jing Zhao,Guoqiang Cao,Jinyu Wang,Qinggang Li,Ping Zheng,Shuxin Zhao,Jibin Sun 한국생물공학회 2018 Biotechnology and Bioprocess Engineering Vol.23 No.1
Phosphoenolpyruvate carboxylase (PEPC) catalyzes the carboxylation of phosphoenolpyruvate (PEP) in the presence of bicarbonate to form oxaloacetate (OAA), and it plays an important role in high-efficient production of OAA-derived metabolites such as lysine, glutamate and succinate. However, PEPCs often suffered from serious feedback inhibition by various metabolic effectors like aspartate. Here, the feedback inhibition of PEPC from Corynebacterium glutamicum was removed by adding a short terminal peptide like His-tag. The effect of His-tag location on the structure and important properties such as activity, thermostability and feedback inhibition of PEPC has been investigated. The purified untagged PEPC, Nterminal His-tagged PEPC (PEPC-N-His) and C-terminal His-tagged PEPC (PEPC-C-His) were characterized. PEPCN- His (439.71/sec/mM) showed a 1.26 and 186-fold higher catalytic efficiency than untagged PEPC (348.59/sec/mM) and PEPC-C-His (2.36/sec/mM), respectively. Both PEPCN- His and untagged PEPC were significantly inhibited by aspartate at the concentrations above 4 mM (residual activities < 10%), while PEPC-C-His was almost desensitized to aspartate within 10 mM (around 90% of residual activity). Structural analysis showed that the extension of C-terminus may cause steric hindrance for aspartate binding with enzymes, leading to the deregulation of feedback inhibition of PEPC-C-His. This study provides a deeper understanding of the effect of terminal fragments on the structure and function of PEPCs, and helps to engineer the feedback inhibition of PEPCs and structurally similar enzymes.
IMPROVING COMPARISON RESULTS ON PRECONDITIONED GENERALIZED ACCELERATED OVERRELAXATION METHODS
Wang, Guangbin,Sun, Deyu The Korean Society for Computational and Applied M 2015 Journal of applied mathematics & informatics Vol.33 No.1
In this paper, we present preconditioned generalized accelerated overrelaxation (GAOR) methods for solving weighted linear least square problems. We compare the spectral radii of the iteration matrices of the preconditioned and the original methods. The comparison results show that the preconditioned GAOR methods converge faster than the GAOR method whenever the GAOR method is convergent. Finally, we give a numerical example to confirm our theoretical results.
Deyu Li,Qin Tong,Yuane Lian,Zhizhong Chen,Yaru Zhu,Weimei Huang,Yang Wen,Qiongyao Wang,Shumei Liang,Man Li,Jianjing Zheng,Zhenhua Liu,Huanxin Liu,Linlang Guo 대한암학회 2021 Cancer Research and Treatment Vol.53 No.4
Purpose Drug resistance is one of the main causes of chemotherapy failure in patients with small cell lung cancer (SCLC), and extensive biological studies into chemotherapy drug resistance are required. Materials and Methods In this study, we performed lncRNA microarray, in vitro functional assays, in vivo models and cDNA microarray to evaluate the impact of lncRNA in SCLC chemoresistance. Results The results showed that KCNQ1OT1 expression was upregulated in SCLC tissues and was a poor prognostic factor for patients with SCLC. Knockdown of KCNQ1OT1 inhibited cell proliferation, migration, chemoresistance and promoted apoptosis of SCLC cells. Mechanistic investigation showed that KCNQ1OT1 can activate transforming growth factor-β1 mediated epithelial-to-mesenchymal transition in SCLC cells. Conclusion Taken together, our study revealed the role of KCNQ1OT1 in the progression and chemoresistance of SCLC, and suggested KCNQ1OT1 as a potential diagnostic and prognostic biomarker in SCLC clinical management.
Improving comparison results on preconditioned generalized accelerated overrelaxation methods
Guangbin Wang,Deyu Sun 한국전산응용수학회 2015 Journal of applied mathematics & informatics Vol.33 No.1
In this paper, we present preconditioned generalized accelerated overrelaxation (GAOR) methods for solving weighted linear least square problems. We compare the spectral radii of the iteration matrices of the preconditioned and the original methods. The comparison results show that the preconditioned GAOR methods converge faster than the GAOR method whenever the GAOR method is convergent. Finally, we give a numerical example to confirm our theoretical results.
Jinju Cui,Deyu Wang,Ning Ma 대한조선학회 2019 International Journal of Naval Architecture and Oc Vol.11 No.1
Based on Nonlinear Finite Element Analysis (NFEA), this paper focuses on the bi-axial ultimate strength of typical bottom structures under corrosion. On one hand, uniform and not simultaneous corrosion across different structures is introduced, and surrogate models by Gaussian Process (GP) are built for both longitudinal and transverse cases individually, and corresponding probabilistic characteristics are investigated; meanwhile, corrosion effects on interaction between bi-axial stresses at ultimate state are studied. On the other hand, non-uniform localized pitting corrosion of normally distributed circular shapes is introduced, and different pitting corrosion densities are considered; structural bi-axial ultimate strengths under pitting corrosion are studied, and the results are compared with that from equivalent uniform corrosion; the probabilistic characteristics of structural ultimate strength in life cycle are studied; finally, the ultimate strength under randomly distributed pitting corrosion is compared with results from normally distributed pitting and uniform corrosion under various boundary conditions.
Cui, Jinju,Wang, Deyu,Ma, Ning The Society of Naval Architects of Korea 2019 International Journal of Naval Architecture and Oc Vol.11 No.1
Based on Nonlinear Finite Element Analysis (NFEA), this paper focuses on the bi-axial ultimate strength of typical bottom structures under corrosion. On one hand, uniform and not simultaneous corrosion across different structures is introduced, and surrogate models by Gaussian Process (GP) are built for both longitudinal and transverse cases individually, and corresponding probabilistic characteristics are investigated; meanwhile, corrosion effects on interaction between bi-axial stresses at ultimate state are studied. On the other hand, non-uniform localized pitting corrosion of normally distributed circular shapes is introduced, and different pitting corrosion densities are considered; structural bi-axial ultimate strengths under pitting corrosion are studied, and the results are compared with that from equivalent uniform corrosion; the probabilistic characteristics of structural ultimate strength in life cycle are studied; finally, the ultimate strength under randomly distributed pitting corrosion is compared with results from normally distributed pitting and uniform corrosion under various boundary conditions.
Study of injection molding process simulation and mold design of automotive back door panel
Huiwen Mao,Youmin Wang,Deyu Yang 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.5
Numerical simulation of the injection molding process of the outer panel of the automotive plastic rear door and mold design is presented here. Computer aided threedimensional interactive application (CATIA) is employed to design the original automotive steel structure, and the modal and thermodynamic properties of the plastic back door outer panel are changed by changing the different injection materials of the back door outer panel. In order to efficiently design the panels, finite element analysis is used to verify whether the designed parts meet the mechanical properties requirements such as light weight, low fuel consumption, short production cycle, strong modeling design, high corrosion resistance and good recovery, the above main parameters have been evaluated, and the above main parameters are carried out evaluate. To simulate the injection molding process, computer aided engineering (CAE) software such as ANSYS and HyperWorks are used to analyze the back door of the selected material. After the numerical analysis, suitable material is selected, so that the modal and thermodynamic properties of the product could be satisfied as well as improved. Unigraphics NX (UG) is employed to design the convex and concave mold for the injection molding of the automobile’s plastic back door panel. Combined with the characteristics of the parts and the design requirements of the injection mold, the multi-scheme design of the pouring and cooling system is carried out. By comparing the effects of different gating and cooling systems on injection molding, the best gating and cooling system is selected. The artificial fish swarm algorithm is used to optimize the process parameters of the injection molding process, and the best combination of the injection molding process parameters of the outer panel of the rear door of the automobile is obtained.
Analytical and numerical analysis for unbonded flexible risers under axisymmetric loads
Guo, Yousong,Chen, Xiqia,Wang, Deyu Techno-Press 2016 Ocean systems engineering Vol.6 No.2
Due to the structural complexity, the response of a flexible riser under axisymmetric loads is quite difficult to determine. Based on equilibrium conditions, geometrical relations and constitutive equations, an analytical model that can accurately predict the axisymmetric behavior of flexible risers is deduced in this paper. Since the mutual exclusion between the contact pressure and interlayer gap is considered in this model, the influence of the load direction on the structural behavior can be analyzed. Meanwhile, a detailed finite element analysis for unbonded flexible risers is conducted. Based on the analytical and numerical models, the structural response of a typical flexible riser under tension, torsion, internal and outer pressure has been studied in detail. The results are compared with experimental data obtained from the literature, and good agreement is found. Studies have shown that the proposed analytical and numerical models can provide an insightful reference for analysis and design of flexible risers.