A novel role for bone-derived cells in ankylosing spondylitis: Focus on IL-23

Jo, Sungsin,Koo, Bon San,Lee, Bitnara,Kwon, Eunji,Lee, Young Lim,Chung, Heekyoung,Sung, Il-Hoon,Park, Ye-Soo,Kim, Tae-Hwan Elsevier 2017 Biochemical and biophysical research communication Vol. No.

<P><B>Abstract</B></P> <P>The main aim of this study are to explore the role of bone-derived cells (BdCs) in ankylosing spondylitis (AS) and determine the underlying molecular mechanisms of IL-23 production. Primary BdCs were isolated from diced bone of facet joints obtained during surgery from seven AS patients and seven disease control (Ct) patients. Osteoblastic activity of BdCs was assessed by measuring their alkaline phosphatase activity and by alizarin red staining. Osteoblast and endoplasmic reticulum (ER) stress-related genes were assessed by quantitative PCR, immunoblotting, immunofluorescence, and immunohistochemistry. In addition, expression of IL-23 in response to BIX (selective BIP inducer X)-induced ER stress was evaluated by qPCR and ELISA. Protein interaction and binding to IL-23 promoter were confirmed by Immunoprecipitation and Chromatin immunoprecipitation, respectively. Transcript levels of genes involved in osteoblast function, as well as of the ER stress marker were higher in the AS group than the Ct group, and elevated RUNX2, BiP and IL-23 expression were observed in the BdCs, serum, and bone biopsies from the AS group. BIX-induced ER stress stimulated osteoblastic activity and IL-23 secretion by upregulating RUNX2 expression. Furthermore, in AS BdCs, RUNX2 interacted with C/EBPβ to bind to IL-23 promoter and RUNX2 knockdown suppressed IL-23 secretion. These finding may provide a molecular mechanism involved in sustained ER stress in AS BdCs stimulates the activation of RUNX2 and C/EBPβ genes, leading to IL-23 production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Bones and its-derived cells from patients with AS showed an increase in ER stress. </LI> <LI> IL-23 cytokine was significantly higher in AS patients than in healthy controls. </LI> <LI> Inducing ER stress in AS exhibited an increase of bone-related genes. </LI> <LI> Inducing ER stress in AS was accompanied with augmentation of IL-23 cytokine. </LI> <LI> ER stress-induced RUNX2 is involved in IL-23 secretion and bone-related genes. </LI> </UL> </P>

Glucocorticoid-induced tumor necrosis factor receptor–related protein co-stimulation facilitates tumor regression by inducing IL-9–producing helper T cells

Kim, Il-Kyu,Kim, Byung-Seok,Koh, Choong-Hyun,Seok, Jae-Won,Park, Jun-Seok,Shin, Kwang-Soo,Bae, Eun-Ah,Lee, Ga-Eun,Jeon, Hyewon,Cho, Jaebeom,Jung, Yujin,Han, Daehee,Kwon, Byoung S,Lee, Ho-Young,Chung, Nature Publishing Group, a division of Macmillan P 2015 Nature medicine Vol.21 No.9

<P>T cell stimulation via glucocorticoid-induced tumor necrosis factor receptor (TNFR)-related protein (GITR) elicits antitumor activity in various tumor models; however, the underlying mechanism of action remains unclear. Here we demonstrate a crucial role for interleukin (IL)-9 in antitumor immunity generated by the GITR agonistic antibody DTA-1. IL-4 receptor knockout (Il4ra(-/-)) mice, which have reduced expression of IL-9, were resistant to tumor growth inhibition by DTA-1. Notably, neutralization of IL-9 considerably impaired tumor rejection induced by DTA-1. In particular, DTA-1-induced IL-9 promoted tumor-specific cytotoxic T lymphocyte (CTL) responses by enhancing the function of dendritic cells in vivo. Furthermore, GITR signaling enhanced the differentiation of IL-9-producing CD4(+) T-helper (T(H)9) cells in a TNFR-associated factor 6 (TRAF6)- and NF-kappa B-dependent manner and inhibited the generation of induced regulatory T cells in vitro. Our findings demonstrate that GITR co-stimulation mediates antitumor immunity by promoting T(H)9 cell differentiation and enhancing CTL responses and thus provide a mechanism of action for GITR agonist-mediated cancer immunotherapies.</P>

Scoparone from Artemisia capillaris Inhibits the Release of Inflammatory Mediators in RAW 264.7 Cells upon Stimulation Cells by Interferon-γ Plus LPS

Seon Il Jang,Young-Jun Kim,Woo-Yiel Lee,Kyung Chell Kwak,Seung Hwa Baek,Gyu Beum Kwak,Young-Gab Yun,Tae-Oh Kwon,Hun Taeg Chung,Kyu-Yun Chai 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.2

Scoparone is a major component of the shoot of Artemisia capillaris (Compositae), which has been used for the treatment of hepatitis and biliary tract infection in oriental countries. In the present study we observed that, scorparone exhibited no cytotoxic effect in unstimulated macrophages, but reduced the release of nitric oxide (NO) and prostaglandin E2 (PGE2) upon stimulation by IFN-γ/LPS or LPS. The inhibitory effects were found to be in conjuction with the suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in IFN-γ/LPS stimulated RAW 264.7 cells. Moreover, scoparone also attenuated the production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 in LPS-stimulated RAW264.7 cells. These results suggest that scoparone decreases the production of the inflammatory mediators such as NO and PGE2 in macrophages by inhibiting iNOS and COX-2 expression.

Scoparone from Artemisia capillaris Inhibits the Release of Inflammatory Mediators in RAW 264.7 Cells upon Stimulation Cells by Interferon-${\gamma}$ Plus LPS

Jang Seon Il,Kim Young-Jun,Lee Woo-Yiel,Kwak Kyung Chell,Baek Seung Hwa,Kwak Gyu Beum,Yun Young-Gab,Kwon Tae-Oh,Chung Hun Taeg,Chai Kyu-Yun The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.2

Scoparone is a major component of the shoot of Artemisia capillaris (Compositae), which has been used for the treatment of hepatitis and biliary tract infection in oriental countries. In the present study we observed that, scorparone exhibited no cytotoxic effect in unstimulated macrophages, but reduced the release of nitric oxide (NO) and prostaglandin $E_2\;(PGE_2)$ upon stimulation by IFN-${\gamma}$/LPS or LPS. The inhibitory effects were found to be in conjuction with the suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in IFN-${\gamma}$/LPS stimulated RAW 264.7 cells. Moreover, scoparone also attenuated the production of tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-$1{\beta}$ and IL-6 in LPS-stimulated RAW264.7 cells. These results suggest that scoparone decreases the production of the inflammatory mediators such as NO and $PGE_2$ in macrophages by inhibiting iNOS and COX-2 expression.

Aberrant expression of interleukin-10 and activation-induced cytidine deaminase in B cells from patients with Behçet's disease

Yoon, Jeong-Yun,Lee, Yeojin,Yu, Seong-Lan,Yoon, Hee-Kyung,Park, Ha-Yan,Joung, Chung-Il,Park, Seok-Rae,Kwon, Mihye,Kang, Jaeku D.A. Spandidos 2017 Biomedical reports Vol.7 No.6

<P>Despite extensive studies, the pathogenesis of Behçet's disease (BD) remains unclear. In particular, the roles of B cells in patients with BD have not been elucidated. Activation-induced cytidine deaminase (AID) is a critical enzyme for immunoglobulin (Ig) heavy chain class switching and somatic hypermutation in B cells and the abnormal expression of AID in various immune conditions has previously been studied. B10 cells, an interleukin (IL)-10-secreting subset of regulatory B cells, function to downregulate inflammation and autoimmunity. Thus, in the present study, the relevance of B cells in patients with BD was investigated. The plasma levels of IL-10 and IgA and the proportions of cluster of differentiation (CD)43<SUP>+</SUP> B cells, excluding naïve B cells, were measured in 16 patients with BD and 16 age- and sex-matched healthy controls (HCs). Additionally, the mRNA levels of IL-10 and AID were assessed in B cells from fresh peripheral blood samples of the BD patients and HCs. The plasma level of IL-10 in patients with BD did not differ significantly from that in HCs. Similarly, there was no significant difference in the plasma level of IgA, although a slight increase was observed in patients with BD compared with that in HCs. There were no differences in CD43<SUP>+</SUP>CD19<SUP>+</SUP> B cell numbers between patients with BD and HCs. However, IL-10 mRNA levels were significantly reduced (P<0.05), while AID mRNA levels were significantly increased (P<0.01) in the B cells of patients with BD compared with those in HCs. These results provide insight into the role of B cells in patients with BD.</P>

ISC3를 이용한 서울시 대기질 모델링

정일래,권오열,안효승,문인수 서울産業大學校 1999 논문집 Vol.49 No.2

In this study ISC3(Industrial Source Complex Ver.3) model is applied to Seoul area (1) to evaluate the applicability of ISC3 by comparing the model results and the measured results under control of Seoul City Government and Han-River Environmental Management Office, and (2) propose the direction of future air pollution management of Seoul area by looking at contributing intensity of point, line and area sources, of which each has different dispersion characteristics, to the air quality. The results of the study, of which pollutant is SO₂ and the year is 1996, are summarized as follows. First, the model results are very similar to those of the measured, showing the regression coefficient(except 4 data of which errors are big) is 0.72 in case of 10 meters height of area source and 0.71 in case of 5 meters height of area source. Therefore ISC3 is judged to be applicable to Seoul area of which comprises of a variety of source types and topographically rolling terrains. Second, for Seoul area, total SO₂emissions are 31.4% from the point sources and the rest 68.6% from the area sources, while contributing weights are 17.39% from the point sources and 82.61% from the area sources in case of 10 meters height of area source, and 16.15% from the point sources and 83.85% from the area sources in case of 5 meters height of area source. Therefore the area sources impact surface concentrations much more than the point sources. Interpreting the second result in terms of contributing intensities which we define the ratio of surface concentration X area and source emissions, contributing intensities of Seoul area are 0.55 for point sources and 1.20 for area sources in case of 10 meters height of area source, and 0.51 for point sources and 1.22 for area sources in case of 5 meters height of area source. Thus the contributing intensity of area sources appears to be approximately as twice as that of point sources. Overall results show that the control of area sources is more urgent and effective than that of point sources in view point of air quality management of SO₂.

예비 과학교사들의 가설 창안 과정에 대한 탐색적 분석

권용주,양일호,정원우 한국교원대학교 과학교육연구소 2000 청람과학교육연구논총 Vol.10 No.1

본 연구에서는 먼저 생물학 관련 내용인 생선같은 어류와 소고기나 돼지고기같은 육상동물 근육의 조직이 서로 차이가 나는 현상에 대한 원인적 가설을 창안하는 기능을 검사하는 도구를 개발하여, 예비 과학교사 23명에게 투입하였다. 그런 다음, 피험자들이 창안한 가설을 정상적 분석 방법으로 분석하여 공통점과 차이점을 분석한 뒤, 가설 및 가설창안과정에서 발견되어지는 경향성(들)을 분석하고자 하였다. 피험자들이 창안한 가설은 모두 총 52개였는데, 본 연구에서 이들을 내용적 속성 측면에서 분석해 본 결과 이들 가설은 크게 조작적 가설 또는 이론적 가설로 분류되었다. 이들이 창안ㄴ한 52개의 가설 가운데 조작적 가설은 45개였고, 나머지 7개는 이론적 가설에 해당되었다. 또한 학생들의 가설창안 과정을 분석해 본 결과, 가설을 창안과정에 사용된 경험적 지식과 새로운 미지의 상황 사이의 유사성 정도에 근거한 외전의 논리적 추론을 중심으로 분석했을 때 4단계로 분류되었다. 특히 피험자들의 가설창안과정의 분석 결과는 약 80%이상이 가설을 창안할 때 기지의 경험적 지식과 미지의 현재 상황과의 유사성 정도를 논리적으로 추론하는 것이 부적절한 모습을 나타내었다. Hypothesis is defined as a single proposition proposed as a possible explanation for the occurrence of some observed phenomena. The purpose of this study was to analyze and categorize hypotheses generated by students on the cause of difference between the structures of muscles of the fishes and the terrestrial animals. A hypothesis-generating test was administered to 23 three college students who were majoring in science education. The study showed that college students generated manipulative and theoretical hypotheses as proposed explanations for the structural difference between muscles of the fishes and the terrestrial animals. Furthermore, students generated several hypotheses which were categorized by the quality of abductive process based on the degree of likeness between experienced knowledge and current phenomena. This study also discusses the implications of these findings for teaching and research in science education.

뇌졸중후 연하곤란 환자의 식이 상태 비교

신용일,양충용,김헌수,강문규,주민철,길은영,권영미 圓光大學校 醫科學硏究所 2004 圓光醫科學 Vol.19 No.2

조직변화의 선행요인에 관한 연구

조정호,권순일 新羅大學校 1999 論文集 Vol.47 No.-

Organizations must be changed if they are to survive, although they oftin appear resistantly to change. And they are frequently transformed into forms remarkably different from the original. The purpose of this paper is studying the framework, the antecedents, and theoretical model of organizational change. The second part of this paper was devoted to concept of organizational change. The concept of organizational change are consisted of the definition and purpose, the typology, the resestance to change and coping strategy, nad the sources of organizational change. The sources of organizational change may be external, internal, or a combination of both. Whether caused by external or internal factors, organizational change invariably affects four independent factorsstructure, task, technology(tools) and people. The third part of thes paper was devoted to discuss the four antecedemts of organizational change-culture of organization, leadership, power(organizational politics) and informational technology. Also, four propositions of organizational change were discussed. Finally based on discussion above I develop the theoretical model of organizational change.

지식경영의 개념에 관한 연구

조정호,권순일 신라대학교 경제경영연구소 2001 경제경영연구 Vol.2 No.-