http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Shin, Dong Hoon,Lin, Haiyue,Zheng, Haifeng,Kim, Kyung Su,Kim, Jin Young,Chun, Yang Sook,Park, Jong Wan,Nam, Joo Hyun,Kim, Woo Kyung,Zhang, Yin Hua,Kim, Sung Joon American Association of Immunologists 2014 Journal of Immunology Vol. No.
<P>The general consensus is that immune cells are exposed to physiological hypoxia in vivo (PhyO(2),2-5% P-O2). However, functional studies of B cells in hypoxic conditions are sparse. Recently, we reported the expression in mouse B cells of TASK-2, a member of pH-sensitive two-pore domain K+ channels with background activity. In this study, we investigated the response of K+ channels to sustained PhyO(2) (sustained hypoxia [SH], 3% P-O2 for 24 h) in WEHI-231 mouse B cells. SH induced voltage-independent background K+ conductance (SH-K-bg) and hyperpolarized the membrane potential. The pH sensitivity and the single-channel conductance of SH-K-bg were consistent with those of TASK-2. Immunoblotting assay results showed that SH significantly increased plasma membrane expressions of TASK-2. Conversely, SH failed to induce any current following small interfering (si)TASK-2 transfection. Similar hypoxic upregulation of TASK-2 was also observed in splenic primary B cells. Mechanistically, upregulation of TASK-2 by SH was prevented by si hypoxia-inducible factor-1 alpha (HIF-1 alpha) transfection or by YC-1, a pharmacological HIF-la inhibitor. In addition, TASK-2 current was increased in WEHI-231 cells overexpressed with 02-resistant HIF-1 alpha. Importantly, [Ca2+](c) increment in response to BCR stimulation was significantly higher in SH-exposed B cells, which was abolished by high K+-induced depolarization or by siTASK-2 transfection. The data demonstrate that TASK-2 is upregulated under hypoxia via HIF-1 alpha dependent manner in B cells. This is functionally important in maintaining the negative membrane potential and providing electrical driving force to control Ca2+ influx.</P>
Su, Chun-Lin,Yang, Jia-Ruei,Kuo, Wen-Ling,Chen, Shin-Cheh,Cheong, David Chon-Fok,Huang, Jung-Ju Korean Society of Plastic and Reconstructive Surge 2021 Archives of Plastic Surgery Vol.48 No.5
Background Direct-to-implant (DTI) breast reconstruction after nipple-sparing mastectomy (NSM) with the use of acellular dermal matrix (ADM) provides reliable outcomes; however, the use of ADM is associated with a higher risk of complications. We analyzed our experiences of post-NSM DTI without ADM and identified the predictive factors of adverse surgical outcomes. Methods Patients who underwent NSM and immediate DTI or two-stage tissue expander (TE) breast reconstruction from 2009 to 2020 were enrolled. Predictors of adverse endpoints were analyzed. Results There were 100 DTI and 29 TE reconstructions. The TE group had a higher rate of postmastectomy radiotherapy (31% vs. 11%; P=0.009), larger specimens (317.37±176.42 g vs. 272.08±126.33 g; P=0.047), larger implants (360.84±85.19 g vs. 298.83±81.13 g; P=0.004) and a higher implant/TE exposure ratio (10.3% vs. 1%; P=0.035). In DTI reconstruction, age over 50 years (odds ratio [OR], 5.43; 95% confidence interval [CI], 1.50-19.74; P=0.010) and a larger mastectomy weight (OR, 1.65; 95% CI, 1.08-2.51; P=0.021) were associated with a higher risk of acute complications. Intraoperative radiotherapy for the nipple-areolar complex increased the risk of acute complications (OR, 4.05; 95% CI, 1.07-15.27; P=0.039) and the likelihood of revision surgery (OR, 5.57; 95% CI, 1.25-24.93; P=0.025). Conclusions Immediate DTI breast reconstruction following NSM is feasible in Asian patients with smaller breasts.
( Yong-woo Shin ),( Deok Bum Park ),( Myung-min Choi ),( Jeong-hoon Chun ),( Baik-lin Seong ),( Gi-eun Rhie ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.3
Molecular imaging is a powerful method for tracking various infectious disease-causing pathogens in host organisms. Currently, a dual molecular imaging method that can provide temporal and spatial information on infected hosts at the organism, organ, tissue, and cellular levels simultaneously has not been reported for Burkholderia pseudomallei, a high-risk pathogen that causes melioidosis. In this study, we have established an experimental method that provides spatiotemporal information on infected hosts using luminescent and fluorescent dual-labeled B. pseudomallei. Using this method, we visualized B. pseudomallei infection at the organism, organ, and tissue levels in a BALB/c mouse model by detecting its luminescence and fluorescence. The infection of B. pseudomallei at the cellular level was also visualized by its emitted fluorescence in infected macrophage cells. This method could be an extremely useful and applicable tool to study the pathogenesis of B. pseudomallei-related infectious diseases.
COVID-19 감염병 대응 의료진용 개인보호복 착용 시 온열 심리반응: 기온과 보호수준의 영향
이도신(Do-Shin Lee),임가영(Ga-Young Lim),이혜린(Hye-Lin Lee),전영민(Young-Min Chun),이주영(Joo-Young Lee) 한국생활환경학회 2021 한국생활환경학회지 Vol.28 No.6
We aimed to evaluate discomfort levels while wearing personal protective equipment (PPE) of healthcare workers to protect from COVID-19 in a hot and humid environment. Six types of PPE (0.18~1.77 clo in thermal insulation; 0.227 ~ 0.319 kPa·m²·W<SUP>-1</SUP> in water evaporative resistance) were chosen and eight subjects (23.1 ± 3.3 y in age) were participated in a neutral (25ºC, 50%RH) and a hot and humid environment (33ºC, 70%RH). A trial consisted of 90 min (10-min rest, 60-min walking and 20-min recovery). Thermal sensation, thermal comfort, humidity sensation, shortness of breath and ratings of perceived exertions (RPE) were recorded. The results showed that the PPE were classified as three levels in terms of discomfort in 33ºC and 70%RH (Low, Middle, and High). Local thermal comfort, shortness of breath and RPE were more valid to classify the discomfort level, rather than overall thermal sensation or comfort. Furthermore, there were no relationships between thermal insulation (or water evaporative resistance) and overall thermal sensation or comfort. Using a powered air purifying respirator was beneficial in the neutral environment only. To conclude, the three levels of discomfort can be applied to improve subjective perceptions of healthcare workers who wear PPE in hot and humid environments.
Accurate and Robust Surface Measurement Using Optimal Structured Light Tracking Method
WANG, Shi,KIM, Hyongsuk,LIN, Chun-Shin,CHEN, Hongxin The Institute of Electronics, Information and Comm 2010 IEICE transactions on information and systems Vol.93 No.2
<P>Structured light vision systems are based on the fact that the pixel location of light in an image has a unique association with the object depth. However, their applications are mainly limited to the properties of the object surface and the lighting conditions. This paper presents a robust vision system for accurate acquisition of 3-D surface data based on optimal structured light. To achieve depth measurement for a dynamic scene, the data acquisition must be performed with only a single image. Our special arrangement makes the image of the light stripe remaining sharp while the background becomes blurred. Moreover, a dynamic programming approach is proposed to track the optimal path while the laser beam is invisible or divergent under extreme condition. The principle and necessary mathematics for implementing the algorithm are presented. The robustness of the system against uncalibrated errors is demonstrated.</P>