Aneuploid Production from Crosses with Diploid and Triploid in Apple Tree

Chun Hua Zhang,Sung Min Park 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.3

The occurrence frequency of aneuploid seedlings in progenies of open pollinated apple triploid, 3x × 2x, 2x × 3x, and 3x × 3x crosses was investigated, and their potentials for genetic studies and breeding were evaluated. The results showed that in the cross combination of open pollinated triploid, 2x × 3x, 3x × 2x, and 3x × 3x, somatic chromosome numbers of the 242 seedlings ranged from 34 to 68. The aneuploid seedlings having chromosome numbers from 36 to 43 appeared with high frequencies, and the seedlings having 38 chromosomes showed the highest frequency. Of these seedlings, four single trisomic seedlings (2n = 2x + 1 = 35) were detected. Three of the 242 seedlings with 54 (3x + 3), 55 (3x + 4), and 68 (4x) chromosomes were considered to have originated from the unreduced gametes (2x + 3, 2x + 4, and 3x) of triploids. Embryos obtained from open pollinated triploid were a little lighter than those of open pollinated diploids. The embryos which were lower than 15 ㎎ did not germinate, due to their abortion. The leaf size of aneuploid seedlings was especially small. Triploid is a valuable material for producing aneuploids, and aneuploid has great potentials on cytogenetic and genetic studies in apple breeding because of its special characteristics.

Growth and Fruit Characteristics of Aneuploid Apple Obtained from Crosses between Diploid and Triploid

Chun-Hua Zhang(장춘화),Sung-Min Park(박성민) (사)한국생물환경조절학회 2009 시설원예‧식물공장 Vol.18 No.4

본 연구는 이전에 이배체와 삼배체를 교배하여 얻은 이수체 사과 나무의 생장과 과실 특성을 알아보고자 수행하였다. 이수체 사과나무의 화분발아율, 영양생장, 과실의 특징을 조사하였다. 그 결과, 이수체 사과 나무 간의 화분발아율의 차이가 크고, 모든 이수체 사과 나무의 화분 발아율이 이배체 ‘홍로’(82.4%) 보다 낮은 것으로 나타났다. 이수체 사과 나무 과실의 평균 크기와 중량은 이배체 ‘홍로’보다 작고 가벼운 것으로 나타났다. 또한 이수체 사과 나무 과실의 길이와 너비에 의해 이수체 JF3942빼고 다른 이수체 과실의 외형이 조금 납작했다. 거의 모든 이수체 사과 나무 과일의 당 함량은 이배체 ‘홍로’보다 높았다. 본 실험에서 얻은 이수체 사과의 산 함량은 이전에 대부분의 연구자의 이배체 사과 결과와 완전히 달랐다. 이수체 사과의 시트르산하고 타르타르산 함량은 사과산 함량보다 더 높은 것으로 나타났다. 이수체 사과 나무의 수고와 폭은 이배체 ‘홍로’보다 짧았다. 대부분 이수체 사과 나무의 수고는 폭보다 길지만 네 나무는 반대로 나타났다. 잎의 길이와 너비에 의해 이수체 사과 나무 잎의 크기가 이배체 ‘홍로’보다 작았다. 이수체 사과 나무는 이배체보다 잘 자라지 않는 것으로 나타났다. This study was conducted to observe some characteristics of aneuploid trees obtained from the crosses between diploid and triploid in the first experiment. The results showed that the difference among the percentage of aneuploid’s pollen grain germination was large, and all of them were lower than that of diploid ‘Hongro’ (82.4%). The average weight and size of each aneuploid's fruit was far lighter and smaller than that of diploid ‘Hongro’. According to the width and length, all of the fruits shape was flat or short-globose conical shape except for JF3942. Almost all the aneuploid had higher sugar content than ‘Hongro’, nearly half of aneuploid fruits were firmer than that of diploid ‘Hongro’. As for the acid contents of aneuploid fruits, the results were greatly different from those of previous studies on diploid apples, in present study the citric acid and tartaric acid contents were more than the malic acid which are dominant acid in most cultivars of apples. Both the length and width of aneuploid tree were shorter than that of diploid ‘Hongro’. Most aneuploid trees’ height was longer than width, but there were 4 exceptions. The size of leaves was smaller than that of diploid ‘Hongro’ according to the length and width of leaves measured. In all, aneuploid’s vegetative growth is not as vigorous as diploid.

Clinical Manifestations and Imaging Characteristics of Gliomatosis Cerebri with Pathological Confirmation

Zhang, Chun-Pu,Li, Hua-Qing,Zhang, Wei-Tao,Liu, Ming-Hui,Pan, Wen-Jing Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

Objective: To explore the clinical manifestations and imaging characteristics of gliomatosis cerebri to raise the awareness and improve its diagnostic accuracy for patients. Materials and Methods: Clinical data, imaging characteristics and pathological examination of 12 patients with GC from Jan., 2008 to Jan., 2012 were analyzed retrospectively. Results: Patients with GC were clinically manifested with headache, vomiting, repeated seizures, fatigue and unstable walking, most of whom had more than 2 lesions involving in parietal lobe, followed by temporal lobe, frontal lobe, periventricular white matter and corpus callosum. Magnetic resonance imaging (MRI) showed diffuse distribution, T1-weighted images (T1WI) with equal and low signals and T2-weighted images (T2WI) with bilateral symmetrical high diffuse signals. There was no reinforcement by enhancement scanning and signals were different in diffusion-weighted images (DWI). The higher the tumor staging, the stronger the signals. Pathological examination showed neuroastrocytoma in which tumor tissues were manifested by infiltrative growth in blood vessels and around neurons. Conclusions: In clinical diagnosis of GC, much attention should be paid to the diffuse distribution of imaging characteristics, incomplete matching between clinical and imaging characteristics and confirmation by combining with histopathological examination.

Fertility and Chromosome Numbers of Aneuploid Apple Crossed with Diploid

Chun Hua Zhang,Sung Min Park 한국원예학회 2009 한국원예학회 학술발표요지 Vol.2009 No.5

Aneuploid Production from Crosses with Diploid and Triploid in Apple Tree

Chun Hua Zhang,Sung Min Park(박성민) 한국원예학회 2010 한국원예학회 학술발표요지 Vol.2010 No.5

Food Microbiology and Biotechnology : Potential Probiotic Characterization of Lactobacillus plantarum Strains Isolated from Inner Mongolia “Hurood” Cheese

( Jian Zhang ),( Xue Zhang ),( Li Zhang ),( Yu Juan Zhao ),( Chun Hua Niu ),( Zhen Nai Yang ),( Sheng Yu Li ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.2

Total 121 lactic acid bacteria were isolated from homemade Inner Mongolia extra hard Hurood cheese. Seven of these strains, identified as Lactobacillus plantarum, were studied for probiotic characteristics. All seven strains survived at pH 3.0 for 3 h, or in the presence of oxgall at 0.3% or 0.6% for 4 h, but their viabilities were affected to different extents at pH 2.0 for 3 h. Strains C37 and C51 showed better adherence to Caco-2 cells, and higher hydrophobicity. The seven L. plantarum strains were different in in vitro free radical scavenging activities and cholesterolreducing ability. In vivo evaluation of the influence of L. plantarum C37 on the intestinal flora in a mouse model showed strain C37 could increase the viable counts of lactobacilli in feces of mice and decrease the viable counts of enterococci. When L. plantarum C37 was used to prepare probiotic Hurood cheese, it was able to maintain high viable counts (>7.8 log CFU/g) during the whole storage period, but the composition of the cheese was not changed. These results indicate that L. plantarum C37 could be considered as a promising probiotic strain.

MSP58 Knockdown Inhibits the Proliferation of Esophageal Squamous Cell Carcinoma in Vitro and in Vivo

Xu, Chun-Sheng,Zheng, Jian-Yong,Zhang, Hai-Long,Zhao, Hua-Dong,Zhang, Jing,Wu, Guo-Qiang,Wu, Lin,Wang, Qing,Wang, Wei-Zhong,Zhang, Jian Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

Esophageal carcinoma (EC) is one of the most aggressive cancers with a poor prognosis. Understanding the molecular mechanisms underlying esophageal cancer progression is a high priority for improved EC diagnosis and prognosis. Recently, MSP58 was shown to behave as an oncogene in colorectal carcinomas and gliomas. However, little is known about its function in esophageal carcinomas. We therefore examined the effects of MSP58 knockdown on the growth of esophageal squamous cell carcinoma (ESCC) cells in vitro and in vivo in order to gain a better understanding of its potential as a tumor therapeutic target. We employed lentiviral-mediated small hairpin RNA (shRNA) to knock down the expression of MSP58 in the ESCC cell lines Eca-109 and EC9706 and demonstrated inhibition of ESCC cell proliferation and colony formation in vitro. Furthermore, flow cytometry and western blot analyses revealed that MSP58 depletion induced cell cycle arrest by regulating the expression of P21, CDK4 and cyclin D1. Notably, the downregulation of MSP58 significantly inhibited the growth of ESCC xenografts in nude mice. Our results suggest that MSP58 may play an important role in ESCC progression.

Selaginella austrotibetica (Selaginellaceae), a new spikemoss from Xizang, China

Meng-Hua Zhang,Jie Yang,Aleksandr Petrovich Shalimov,강종수,Xian Chun Zhang 국립생물자원관 2024 Journal of species research Vol.13 No.1

Selaginella austrotibetica, a new spikemoss species from China, is described. The species is epipetric and was found on a moss-covered rocky cliff by a riverbank. Morphologically, it is unlike any anisosporophyllous species in the genus Selaginella. However, it resembles species of the S. sanguinolenta group, especially similar to S. sajanensis in gross morphology, except for the characters of strobilus. The molecular phylogenetic analysis clearly showed that S. austrotibetica was nested within the anisosporophyllous species clade, forming a sister relationship with the clade of S. vaginata and S. xipholepis, and distinct from the S. sanguinolenta group, including S. sajanensis. Although S. austrotibetica is sister to the clade of S. vaginata and S. xipholepis, the species can be clearly distinguished by its sparse leaves on branches, dorsal leaves that are broad-ovate, base obtuse to rounded, margin denticulate or very shortly sparsely ciliolate, and leaves that are slightly thick and shiny. Therefore, we described Selaginella austrotibetica as a new species and discuss its phylogenetic relationships within the genus Selaginella.

리더십 유형이 직무만족 및 조직몰입에 미치는 영향에 관한 연구 -중국 특급호텔을 중심으로-

장춘화 ( Chun Hua Zhang ),황순애 ( Soom Ae Hwang ) 한국항공경영학회 2013 한국항공경영학회 춘계학술대회 Vol.2013 No.-

This study, hotel managers or any type of leadership for the members of the organization while achieving a high job satisfaction, commitment and service quality in hotel industry and hotel business management and investigated whether help. This research work in five-star hotel in Beijing, and type of employees to target leadership Transactional leadership, Transformational leadership and organizational commitment of employees and the impact on job satisfaction were analyzed. Questionnaire survey period, 2011 October 10 to November 6 Total 330, and 263 Department of wealth by distributing a questionnaire survey was used in the empirical analysis. Data analysis program SPSS WIN Version 18.0 statistical package was analyzed using frequency analysis, reliability analysis, factor analysis, regression analysis was verified by.

Selaginella subvaginata (Selaginellaceae), a new spikemoss from China

Xian-Chun Zhang,Aleksandr Petrovich Shalimov,강종수,Meng-Hua Zhang 국립생물자원관 2020 Journal of species research Vol.9 No.3

Selaginella vaginata is a common montane species with broad distribution in China and the Himalaya region, and several species that are morphologically similar to S. vaginata are distributed in Asia. The taxonomic revision of S. vaginata and related species was performed by morphological comparison of leaves, strobili, and spores, and phylogenetic analysis. Based on these results, a new species, S. subvaginata, sp. nov., has been identified. Morphologically, S. subvaginata has intermediate form between S. vaginata and S. repanda, which differs mainly in its main stem being erect, dorsal leaves long-ciliolate on inner margin and outer margin denticulate or with 2-4 cilia at base (long-ciliolate on both inner and outer margins in S. vaginata, denticulate on both inner and outer margins in S. repanda), and acroscopic base of ventral leaves long ciliolate (sparsely long ciliolate in S. vaginata, short ciliolate to denticulate in S. repanda). Moreover, phylogenetic analysis using three chloroplast markers (rbcL, atpI, and psbA) revealed that S. subvaginata is a distinct species among the anisosporophyllous species clade in Selaginellaceae.