Genome-wide investigation of a Korean synthetic breed, Woori-Heukdon using the Illumina PorcineSNP60K BeadChip

Yong‑Min Kim,Ha‑Seung Seong,Jung‑Jae Lee,Da‑Hye Son,Jin‑Su Kim,Soo‑Jin Sa,Young‑Sin Kim,Tae‑Jeong Choi,Kyu‑Ho Cho,Joon‑Ki Hong,Jung‑Woo Choi,Eun‑Seok Cho 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Woori-Heukdon (KWH) is a Korean synthetic pig breed generated using Chookjin-Duroc (KCD), Chookjin-Chamdon (KCC), and their crossbreds. Currently, there is a severe lack of studies investigating the Korean breed populations including wild boars (KWB) throughout the genome. Objective: This study was performed to investigate the genetic characteristics of Korean pig populations at the genome-wide level. Methods: Using the SNP dataset derived from genotyped and downloaded datasets using the Illumina PorcineSNP60K BeadChip, we compared the genomes of 532 individuals derived from 23 pig breeds to assess the genetic diversity, inbreeding coefficient, genetic differentiation, and population structure. Results: KWB showed the lowest average expected heterozygosity (HE = 0.1904), while KWH showed the highest genetic diversity (HE = 0.02859) among Korean populations. We verified that the genetic composition of KWH, showing USD of 74.8% and KCC of 25.2% in ADMIXTURE analysis. In population structure analyses, KCC was consistently shown to be separated from other pig populations. In addition, we observed gene flow from Western pigs to a part of Chinese populations. Conclusion: This study showed that Korean native pigs, KCC have genetic differences in comparison with Chinese and Western pigs; despite some historical records and recent genetic studies, we could not find any clear evidence that KCC was significantly influenced by Chinese or Western breeds in this study. We also verified the theoretical genomic composition of KWH at the molecular level in structure analyses. To our knowledge, this is the first genomic study to investigate the genomic characteristics of KWH and KCC.

정준상관 행렬도와 군집분석을 응용한 KLPGA 선수의 기술과 경기성적요인에 대한 연관성 분석

최태훈,최용석,Choi, Tae-Hoon,Choi, Yong-Seok 한국통계학회 2008 응용통계연구 Vol.21 No.3

정준상관 행렬도(canonical correlation biplot)는 정준상관분석에서 두 변수 집단에 의해서 측정된 다변량 자료에서 변수 집단 간의 관계와 개체들의 관계를 탐색하기 위한 2차원 그림이다. 이는 일반적으로 최용석 (2006, 1장)의 한 변수 집단에 의한 행렬자료에 대한 일반적인 행렬도를 두 변수 집단에 의한 행렬자료로 확장한 것으로 볼 수 있다. 최근에 Choi와 Kim (2008)은 개체들이 많은 대용량 자료에서 행렬도의 해석상 힘든 문제점을 지적하고 이를 극복하는 데 군집분석을 활용하는 방법을 제시하고 있다. 일반적인 행렬도에서 발생하는 대용량 자료에 대한문제는 정준상관 행렬도에서도 동일하게 발생하곤 한다. 본 연구에서는 2006년도 KLPGA 선수 중 상금 순위 상위 50명을 대상으로 정준상관 행렬도를 통해 기술요인변수군(평균 퍼팅수. 그린 적중율, 파 세이브율, 파 브레이크율)과 경기성적요인변수군(상금, 평균 타수)간의 관련성을 살펴보고 군집분석을 활용하여 각 선수들의 군집을 시도하려한다. Canonical correlation biplot is 2-dimensional plot for investigating the relationship between two sets of variables and the relationship between observations and variables in canonical correlation analysis graphically. In general, biplot is useful for giving a graphical description of the data. However, this general biplot and also canonical correlation biplot do not give some concise interpretations between variables and observations when the number of observations are large. Recently, for overcoming this problem, Choi and Kim (2008) suggested a method to interpret the biplot analysis by applying the K-means clustering analysis. Therefore, in this study, we will apply their method for investigating the relationship between skill and competition score factors of KLPGA players using canonical correlation biplot and cluster analysis.

4극 이방성 Sr페라이트·플라스틱자석의 제조에 관한 연구

최희태,문현욱,신용진,진성빈 明知大學校 産業技術硏究所 1995 産業技術硏究所論文集 Vol.14 No.-

This thesis deals with the fabrication of 4 poles anisotropic Sr ferrite plastic magnets. After Polyamide6 and polyamide12 are kneaded respectively with Sr ferrite powder, silane coupling and calcium stearate of lwt% are added for coating and pelleting. The pelleted specimen injection-moulded under magnetic field using 4 poles mould. In the case of using polyamide6 as a binder, for 4 poles anisotropic Sr ferrite plastic magnets the surface magnetic flux density distribution is +943.8∼-943.8kG and the deviation is 5.2%, polyamide12, the distribution is +1040.9∼-1040.9KG and the deviation is 5.4%.The magnet distribution shows stability. As the results of experiments, we find 4 poles anistropic Sr ferrite plastic magnets have properties as the materials appropriate for manufacturing magnet type synchronous motors.

성인의 경부 전면 중앙부위에서 발생한 기형종 1예

최월용,정효진,채종인,하태인,정상렬,이광재,채병도,최영식 고신대학교 의학부 2004 高神大學校 醫學部 論文集 Vol.19 No.1

Teratomas are embryonal neoplasms that show characteristics of three germ cell layers(ectoderm, mesoderm, and endoderm). They are distributed in the following regions: sacrococcygeal(57%), gonadal(29%), mediastinal(7%), cervical(3%), retroperitoneal(4%), and intracranial(3%) regions. Non-gonadal treatomas present in infancy whereas gonadal are seen in adolescence. Especially, teratomas that occurred in midline neck of adult are very rare and are confused with thyroglossal duct cysts, cystic hygromas, and thyroid tumors. We report, along with the review of appropriate literature, a case of histopathologically confirmed benign teratoma in the midline neck of a 33 years old woman.

Cone beam형 전산화단층영상의 원리

최용석,김규태,황의환 대한구강악안면방사선학회 2006 Imaging Science in Dentistry Vol.36 No.3

The use of computed tomography for dental procedures has increased recently. Cone beam computed tomography (CBCT) systems have been designed for imaging hard tissues of the dentomaxillofacial region. CBCT is capable of providing high resolution in images of high diagnostic quality. This technology allows for 3-dimensional representation of the dentomaxillofacial skeleton with minimal distortion, but at lower equipment cost, simpler image acquisition and lower patient dose. Because this technology produces images with isotropic sub-millimeter spatial resolution, it is ideally suited for dedicated dentomaxillofacial imaging. In this paper, we provide a brief overview of cone beam scanning technology and compare it with the fan beam scanning used in conventional CT and the basic principles of currently available CBCT systems.

CRP* 의존성 maltose 대사 촉진 유전자 sfs4의 클로닝 및 염기배열 결정

최용락,정희태,조무제,정수열 東亞大學校附設遺傳工學硏究所 1996 遺傳工學硏究 Vol.- No.3

CRP (cAMP receptor protein)은 cAMP와 결합하여 cAMP-CRP 복합체를 형성하여 전사조절의 조절인자로서 작용한다. crp 유전자에 변이를 도입하여 cAMP의 비존재 상태에서 cAMP-CRP와 비슷한 기능을 가진 crp* 유전자가 도입된 대장균 MK2001 (crp*¹, cya::km)을 숙주로 사용하여 cAMP 혹은 cGMP의 비존재하에서도 mal 유전자의 발현을 촉진시키는 유전자 sfs (sugar fermentation stimulation) 수 종을 클로닝하였다. 본 실험에서는 이미 밝혀진 nlp (Ner like protein) 유전자와 같이, sfs의 새로운 유전자를 탐색하여, 그 중 sfs4의 2126bp 전 염기배열을 결정하고, 잠정적인 sfs4의 promoter 영역에는 CRP 단백질과의 결합 DNA 공통 염기배열(5' AAT TGTGA ACACCA TCACC CGT 3')이 존재함을 확인했다. lacZ 융합 유전자를 작성하여 TP2010R1과 MK2001의 균주에서 cAMP를 첨가할 경우 각각 2.3배, 1.8배의 β-galactosidase 활성이 증가 하는 것으로 보아 sfs4는 cAMP-CRP에 의해 발현 조절을 받는 것으로 나타났다 In Escherichia coli, CRP forms a complex with cAMP and acts as a transcriptional regulator of many genes, including sugar metabolism operons. The E. coli MK2001, which is introduced the altered crp*¹, is functional in the expression of lac, ara and man, in the absence of cAMP. However, the expression of mal gene is fully activated by the addition of cAMP or cGMP. The object of the study is cloning of the sfs (sugar fermentation stimulation) genes, which was involved in regulation of mal gene expression with the altered crp*¹gene, and structural analysis and characterization of the genes at the molecular level. We have cloned 5 different E. coli genes which stimulate the maltose metabolism in a crp*¹, cya::km (MK2001) background. Newly identified genes were designated as sfs. One of the sfs genes (pPC1), located at the 53.2 min map position in the E. coli chromosome, was further analyzed. Expression of the genes, which is involved in maltose metabolism, malQ (amylomaltase), was increased to 5.8-fold in the presence of a plasmid, pAP5, containing the subcloned sfs4 gene. The nucleotide sequence of a common 2,126bp segment of the pPCM1 was determined and two open reading frames (ORF1 and ORF2) were detected. The ORF1 encodes the sfs4 gene and ORF2 encodes a truncated protein. Potential CRP binding site is located in the upstream of the putative promoter in the regulatory region. Expression of the cloned sfs4 gene was positively regulated by the cAMP-CRP complex.

신생아의 두뇌 초음파검사상 뇌실주위 고반향음영에 관한 연구

최영칠,이태용,조영채,이동배 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Neurosonography is an excellent modality for imaging a wide array of intracranial pathology of the infant and the newborn. Periventricular high echos were prominent for the premature infant, but there are some difficulties in differentiate normal periventricular high echo from periventricular leukomalacia. White matter indices on neurosonography of 152 healthy newborn babies were measured and analized, with their gestational age, method of delivery, birth weight, APGAR score(1 min and 5 min), diagnosis and labaratory data. The gestational age of babies at birth (p=0.0001) and the delivery with Caesarian section (p=0.0263) were related statistcally to high white matter index. The babies with metabolic disease had higher white matter index than other disease group or normal control group. On the basis of these results, We speculate that in addition to gestational age of infants, delivery method and their disease status may play an additional role in presenting ldgh periventricular echo on neurosonography. It is possible to predict the size of non pathologic periventricular high echo and to give more information for further study plan.

독일의 경미범죄의 처리제도와 구체적 운영실태에 관한 연구

최영규,하태영 慶南大學校 法學硏究所 2005 慶南法學 Vol.20 No.-

非晶質滋性合金薄帶의 滋歪測定에 관한 硏究

崔熙太,愼鏞璡,徐康壽 明知大學校 産業技術硏究所 1989 産業技術硏究所論文集 Vol.8 No.-

This paper is studied on the measurement of magnetostriction of an alloy ribbons tensile force, via measuring the response ar the double frequency of the driving field frequency. The experiments with both crystalline magnetic material,(??)-Fe and amorphous magnetic material. ??, proved that the possibility in this paper can measure magnetostriction with ease and high accuracy.

표고 폐골목으로 제조한 목질보드의 성질

최용순,황원중,한태형,김남훈,권진헌 江原大學校 森林科學硏究所 1998 Journal of Forest Science Vol.14 No.-

要約표고폐골목의 재활용을 목적으로 폐골목과 정상재의 칩을 일정비율로 혼합하여 얻은 목질보드의 성질을 조사, 검토하였다. 그 결과 폐골목의 목섬유는 정상재보다 다소 짧고 표 면이 거칠었으며 폐골목 변재부의 밀도는 정상재에 비해 상당히 낮았다. 파티클보드의 두께 팽윤율 및 파괴계수의 시험결과 폐골목을 25~50% 정도 혼합한 경우는 정상재와 비슷한 값 을 나타냈다.결론적으로 폐골목은 파티클보드의 재료로 사용될 수 있고 정상재와의 혼합비율은 25~50% 범위에서 조정되어야 할 것으로 생각되었다.ABSTRACTTo provide further information for reutilization of the bed-logs decayed by oak mushroomfLentinus edodes), the properties of wood-based board prepared with mixed materials of normal wood and bed-logs were examined.Wood fibers in bed-logs showed different morphology from normal wood fibers, that is, shorter length and rough surface. Thickness swelling increased with increasing mixing ratio of bed-log. On the other hand, modulus of rupture(MOR) in bending strength decreased. In products, the visual surface of board prepared from higher mixing rate of bed-log chips was smooth.Consquently, it is considered that the optimum mixing rate of bed-log to normal wood is the range of 25-50% and the bed-log can be used for raw material of board instead of normal wood.