Resistance-breaking ability and feeding behavior of the brown planthopper, Nilaparvata lugens, recently collected in Korea

Bo Yoon Seo,Jin Kyo Jung,Byeong-Reol Choi,Hyung Man Park 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.05

Resistance-breaking ability of wild brown planthopper (BPH), Nilaparvata lugens, on resistant rice cultivars has been reported in many Asian countries. To understand the development of resistance-breaking ability of wild BPH in Korea, we conducted the nymphal survivorship test and the electrical penetration graph (EPG) on susceptible and resistant rice cultivars with four different BPH populations, which were collected in the early 1980s (S-BPH), 2005, 2006, and 2007, respectively. The S-BPH showed low survival rates on resistant rice cultivars carrying Bph1 and bph2, respectively. On the other hand, recent(2005~2007) wild BPH populations seemed to have high resistance-breaking ability because they maintained elevated survival rates on most other resistant rice cultivars except Gayabyeo (Bph1 and bph2) and Rathu Heenati (Bph3). In the EPG monitoring, however, wild BPHs could not easily feed on the phloem sap of resistant rice cultivars, Cheongcheongbyeo (Bph1), ASD7 (bph2), and Gayabyeo. Wild BPHs spent more time on reaching the phloem sieve elements of resistant cultivars. Ph waveform duration and honeydew excretion amount of wild BPHs also decreased. From the results, we suggest that though recent wild BPHs collected in Korea have high resistance-breaking ability simultaneously on rice cultivars carrying Bph1 and bph2 through the increase of survivorship, they still have to pay some cost to feed on the phloem sap of resistant rice cultivars.

Survival rate and stylet penetration behavior of current Korean populations of the brown planthopper, Nilaparvata lugens, on resistant rice varieties

Bo Yoon Seo,정진교,Byeong-Reol Choi,Hyung-Man Park,Si-Woo Lee,Bong Hoon Lee 한국응용곤충학회 2010 Journal of Asia-Pacific Entomology Vol.13 No.1

To understand the development of host plant resistance-breaking ability of the current BPH populations in Korea, we conducted nymphal survivorship tests and electrical penetration graph (EPG) studies on susceptible and resistant rice varieties with four different BPH populations, which were collected in the early 1980s (S-BPH) and in 2005, 2006, and 2007. The S-BPH had low survival rates on resistant rice varieties carrying either Bph1 or bph2. However, the current BPH populations have high resistance-breaking ability on the varieties with their elevated survival rates, whereas their survival rates were still low on the other resistant varieties, Gayabyeo (Bph1 the other unknown gene) and Rathu Heenati (Bph3). The EPG analysis also revealed that the ratio of BPH that could reach the phloem sap ingestion waveform (N4-b) within 15 h on the resistant rice varieties containing Bph1 or bph2 was higher in the current BPH populations (16.7–50%)than in the S-BPH population (0-4.2%). However, the pre-reaching time from the penetration start to the first N4-b waveform in the current BPH populations was significantly longer on resistant varieties (Bph1 or bph2)than on susceptible varieties. Furthermore, the total duration of N4-b waveform was significantly shorter on the resistant varieties. From these results, we suggest that, although the current BPHs collected in Korea have a high resistancebreaking ability through the increase of survival rate on resistant rice varieties carrying either Bph1 or bph2,they still have some difficulties feeding on the phloem sap of the resistant rice varieties.

Survival rate and stylet penetration behavior of current Korean populations of the brown planthopper, Nilaparvata lugens, on resistant rice varieties

Seo, Bo-Yoon,Jung, Jin-Kyo,Choi, Byeong-Reol,Park, Hyung-Man,Lee, Si-Woo,Lee, Bong-Hoon 한국응용곤충학회 2010 Journal of Asia-Pacific Entomology Vol. No.

To understand the development of host plant resistance-breaking ability of the current BPH populations in Korea, we conducted nymphal survivorship tests and electrical penetration graph (EPG) studies on susceptible and resistant rice varieties with four different BPH populations, which were collected in the early 1980s (S-BPH) and in 2005, 2006, and 2007. The S-BPH had low survival rates on resistant rice varieties carrying either Bph1 or bph2. However, the current BPH populations have high resistance-breaking ability on the varieties with their elevated survival rates, whereas their survival rates were still low on the other resistant varieties, Gayabyeo (Bph1 the other unknown gene) and Rathu Heenati (Bph3). The EPG analysis also revealed that the ratio of BPH that could reach the phloem sap ingestion waveform (N4-b) within 15 h on the resistant rice varieties containing Bph1 or bph2 was higher in the current BPH populations (16.7-50%) than in the S-BPH population (0-4.2%). However, the pre-reaching time from the penetration start to the first N4-b waveform in the current BPH populations was significantly longer on resistant varieties (Bph1 or bph2) than on susceptible varieties. Furthermore, the total duration of N4-b waveform was significantly shorter on the resistant varieties. From these results, we suggest that, although the current BPHs collected in Korea have a high resistance-breaking ability through the increase of survival rate on resistant rice varieties carrying either Bph1 or bph2, they still have some difficulties feeding on the phloem sap of the resistant rice varieties.

온도별 구리금파리(Lucilia sericata)의 발육

김현철,김수정,윤지은,조태호,최병렬,박정규,Kim, Hyeon-Cheol,Kim, Soo-Jung,Yun, Ji-Eun,Jo, Tae-Ho,Choi, Byeong-Reol,Park, Chung-Gyoo 한국응용곤충학회 2007 한국응용곤충학회지 Vol.46 No.1

Maggot therapy는 1900년대 초부터 압박성 욕창, 당뇨나 괴사에 의한 궤양, 수술 후 감염된 상처, 화상 등의 상처치료에 다시 사용되기 시작하였다. 환부에 처리할 적절한 발육단계의 무균유충을 생산하고, 증식 보존용 계통을 유지하는 데 필요한 자료를 얻기 위하여 온도별(15.4, 20.6, 22.5, 26.2, 29.1, $33.0^{\circ}C$) 발육실험을 수행하였다. 간배지(liver agar medium)에서 알기간과 1령 및 2령 기간은 $33.0^{\circ}C$에서 각각 9.0시간, 14.0시간, 18.6시간으로 가장 짧았다. 3령 기간과 번데기 기간은 $29.1^{\circ}C$에서 각각 285.0시간과 171.0시간으로 가장 짧았다. 온도별 발육기간을 기초로 각 발육단계의 발육영점온도와 유효적산온도를 산출하였다. 암수 성충은 대개 1개월 이상 생존하였고, 암컷은 일평생 2.7회 산란하였으며, 총 338.5개의 알을 낳았다. Maggot therapy (MT) has been re-introduced for non-healing wounds such as pressure sore, diabetic and necrotic ulcers, as well as infected surgical wounds, burn, and trauma injuries since early 1990s. For the production of sterile maggot of proper developmental stage, Lucilia sericata were studied on developmental periods of immature stages on liver agar medium under six different temperatures such as 15.4, 20.6, 22.5, 26.2, 29.1, and $33.0^{\circ}C$, and adult longevity and egg Production under $29^{\circ}C$. The periods of eggs and larvae of the 1st and the 2nd instars were shortest at $33^{\circ}C$ by 9.0, 14.0 and 18.6 hours, respectively. The periods of the 3rd instar larvae and pupae were shortest at $29.1^{\circ}C$ by 285.0 and 171.0 hours, respectively. Developmental zero point and total effective temperature far the development of each stage were calculated based on the developmental periods. Adults of both female and male lived more than one month. Females laid an average of 338.5 eggs through 2.7 times of egg laying throughout her lifetime.

벼먹노린재(Scotinophara lurida)의 월동처와 본답발생

이기열,박성규,안기수,최병렬,Lee Ki-Yeol,Park Sung-Kyu,Ahn Ki-Su,Choi Byeong-Reol 한국응용곤충학회 2004 한국응용곤충학회지 Vol.43 No.4

Overwintering sites and Seasonal occurrence of various growth stage of the rice black bug, Scotinophara lurida BURMEISTER were studied at the rice paddy field in Chungbuk area, from 1999 to 2001. Attraction by light trap from overwintering site of overwintering adults occurred from early June to mid July and its peak was shown on late June. Eggs were oviposited from early July to early August and its peak appeared in late July. Nymphs were observed from mid July to late September with its peak on mid August. The newly eclosed rice black bugs were found in late August and its peak on mid September. The rice black bug overwintered as adult at mountain foot, banks, and rice paddy levee. 충북지역에서 벼먹노린재(Scotinophara lurida)에 대한 월동처와 본논발생에 대하여 1999년부터 2001년에 걸쳐 조사하였다. 벼먹노린재 월동성충은 월동처에서 6월 상순부터 7월 상순까지 유아등에 포획되었으며, 이동최성기는 6월 하순이었다. 본논에서 각 태별 발생 피크는 월동성충이 7월 중순, 알이 7월 하순, 약충은 8월 중순에 가장 높은 밀도를 보였으며, 제 1대 성충은 9월 중순에 가장 높은 밀도 피크를 보였다. 월동태는 성충이며, 월동충은 산기슭, 제방, 논뚝에서 발견되었다.