瓦松의 알콜 抽出物이 心筋 및 腸筋收縮에 미치는 影響

曺石鉉,金光鎭 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.1

Orostachys malacophyllus F. is a kind of ,Chinese medicine to grow on the tiled roof of the house or on the rock of the mountain. The chemical compositions and actions have not been reported, but, sometimes, it was used as a folk remedy for the treatment of cancerous diseases. This study was attempted to investigate the effects of water fraction separated from alcohol extract of Orostachys malacophyllus F. on the contractilities of cardiac and intestinal muscles comparing with those of the water extract. The muscles used for this study were the ventricular muscle of turtle and the intestinal muscle of mouse, and their contractilities were represented as a change of the muscle tension detected by myograph. The results were summarized as follows: 1. The water fraction decreased the contractility of ventricular muscle, and increased the intestinal contractility. But the water extract was observed the opposite effects on the contractility of each muscle. 2. Sympathetic or parasympathetic blocker and alpha-or beta-blocker had no effect on the decreased or increased contractility of each muscle. 3. Calcium antagonist(verapamil) inhibited significantly the contractility in the case of the water fraction to the intestinal muscle and the water extract to the ventricular muscle. From the above results, it was suggested that the action of water fraction or the water extact affected to calcium transport: release into the sarcoplasmic fluid or active influx into the sarcoplasmic reticulum in the case of the cardiac muscle, and release from ECF or cisternae of reticulum into the muscle cell in the smooth muscle.

황토 급여가 거세한우의 발육에 미치는 영향

조원모,기광석,정하연,강수원,김준식,백봉현,김용국 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.1

본 연구는 거세한우 육성우에 대한 황토 첨가급여가 발육에 미치는 효과를 구명하고자 발육능력, 사료섭취량을 규명한 바 아래와 같은 결과를 얻었다. 전 시험기간 동안의 평균 일당증체량은 대조구와 황토급여구가 각각 0.91㎏과 0.87㎏/day로 대조구가 높은 것으로 나타났지만 유의성은 인정되지 않았다. 사료섭취량은 대조구와 황토급여구 모두 평균 일일 두당 농후사료 섭취량이 2.42㎏이었으나, 건초섭취량에 있어서는 각각 3.02와 3.14㎏으로 황토급여구가 다소 높은 경향으로 나타났다. The study was conducted to investigate the effect of dietary supplements of reddish clay on the growth performance in growing Hanwoo steers. A total of 8 calves was allocated into 2 feeding groups, control(basal diet) and treatment(basal diet plus ad libitim Reddish clay) for Hanwoo steers. This study was carried out for 5 months with a total of 8 calves. The results obtained in the present studies are summarized as follows : Average daily gains throughout the experimental period of 4 months to 9 months of age were 0.91 and 0.87㎏ for control group and reddish clay-supplemented group, respectively, however, no statistical significances were found. Average daily intake of concentrates was 2.42㎏ for each group, while that of hay tended to be higher in reddish clay-supplemented group(3.14㎏) than in control group(3.02㎏).

호스피스 시설의 유형별 공간구성에 관한 건축 계획적 연구

조광현,박재승 한국의료복지시설학회 2002 의료·복지 건축 Vol.8 No.1

Recently cancer, AIDS, chronic sickness have increased according to the elevation of socioeconomic level and fast change of lifestyle. The number of patients receiving terminal care increased fairly because the span of life is extended by development of medicinal technology. Also necessity of hospice and palliative care was risen according to the request of terminal patients that remove pain and keep calm life by interest about quality of life, However architectural plan and type specialization of facility which can correspond team's composition and supplied nursing program are not consisting. This study researches about care environment of hospice facility plan through investigation into terminal patient's special quality. The purpose of this study is to propose fundamental datas of hospice facility for architectural plan through comparative analysis of cases of domestic and outside facilities.

Nd:YAG 레이저 조사와 불화물 도포가 탈회법랑질의 재광화에 미치는 영향

조현,이광희,김대업 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.3

Normal pulsed Nd:YAG 레이저 조사와 불소화합물이 산성음료수에 의해 탈회된 법랑질의 표면 미세경도에 미치는 재광화 효과를 평가하고자 한다. 법랑질 시편을 제작하여 50ml 플라스틱 시험관 5개에 코카콜라를 채운 후 30개의 소구치를 6개씩 나누어 넣고 탈회시켰다. 법랑질 표면에 6W 출혁하의 50mJ 에너지 밀도, 20Hz의 pulse rate로 레이저를 조사하였다. 레이저가 조사된 법랑질 표면에 각 군별로 여러 형태의 불소화합물을 처리한 후 탈회 전, 후의 미세경도 및 Diagnodent를 측정하였다. 동일한 방법으로 탈회된 법랑질에 레이저를 조사했을 때와 레이저가 조사된 법랑질 표면에 불소화합물을 처치한 후에 미세 경도 및 Diagnodent를 측정하였다. 법랑질 표면을 산성 음료에 탈회 후, 레이저 조사 후, 불소화합물 처치 후의 각각의 시편에 대하여, 주사전자현미경으로 1500배 확대하여 관찰하였다. 1. 산성 탈회된 법랑질 표면의 미세경도(VHN)가 레이저를 조사한 수와 불소 도포 후에 증가하였으나, 레이저 조사 후의 경도와 불소 도포 후의 경도간의 차이는 유의하지 않았다. 2. 초기 경도, 탈회 후 경도, 레이저 조사 후 경도, 불소 도포 후 경도에서 불소군 간의 차이는 모두 유의하지 않았고, 불소 용액 군과 불소 젤 군에서는 레이저 조사 후의 경도와 불소 도포 후의 경도 간에 유의한 차이가 없었으나, 불소 바니쉬군에서는 유의한 차이가 있었다. 3. Diagnodent 측정치의 변화에서 초기 점수, 탈회 후 점수, 레이저 조사 후의 점수와 불소 도포 후의 점수 간에는 유의한 차이가 있었고, 레이저 조사 후 점수와 불소 도포 후의 점수가 불소 도포 군 사이에서는 유의한 차이가 없었다. 4. 산성 음료수에 탈회된 법랑질 표면에의 Nd:YAG 레이저 조사는 재광화에 효과적이며, 불소화합물의 처치는 레이저의 효과를 증가시키는 부가적인 효과를 갖는다. 5. 주사 전자 현미경상에서 Nd:YAG 레이저가 조사된 법랑질 표면은 전체 표면이 법랑소주가 녹아내려 융합된 매끈한 바닥에 균열이 생기고 분화구가 퍼져 있는 양상을 보였으며, 불소 도포 후에는 균열과 분화구 형태는 뚜렷하지 않으며 탈회된 후의 표면에 가까운 양상을 보였다. The purpose of this in vitro study was to investigate the effects of Nd:YAG laser irradiation and fluorides on acid demineralized enamel. The materials were 30 freshly extracted permanent premolars with intact smooth enamel surfaces. They were demineralized with Coca-cola at 37℃ for 12 hours and then irradiated by Nd:YAG laser with 6W power, 50mJ/㎠ energy density, and 20Hz pulse repetition. After laser irradiation, teeth were treated by three kinds of fluorides; (1) 0.05% NaF fluoride solution (2) 1.23% APF gel and (3) 0.1%F fluoride varnish, microhardness(VHN) and Diagnodent scores were measured and the surfaces of each treated specimens were also observed with SEM under 1500 magnification. The results were as follows: 1. In the change of microhardness(VHN), it decreased to 34.68% from the initial micrhardness, increased to 78.37% after laser irradiation and increased to 82.62% after fluoride treatment, there were significant differences except when it was irradiated and treated with fluoride(P<0.05). 2. In the change of Diagnodent scores, it was decreased to 28.08% from the initial scores after demineralization, and then increased to 59.81% after laser irradiation, and increased to 82.17% after fluoride treatment. Scores were different significantly between the scores of initial, demineralization, laser irradiation and fluoride treatment (P<0.05). All the scores were not different significantly between fluoride types. 3. SEM observation showed that the lased enamel surfaces after demineralization were thermally degenerated and showed molten lava-like appearance and crater with cracks and many microholes.

심실중격결손증-치험 1예-

조광현,우종수,황윤호,이양행,박철호,김종성,김철호,이경순,조영일,박태인 인제대학교 1985 仁濟醫學 Vol.6 No.4

A successful treatment of infants and children with congenital heart defects depends upon several factors: diagnosis must be accurate, the operative technique, including techniques of perfusion and myocardial preservation, must be suited to the needs of patients and intensive cares, including accurate anesthesia, must be applied through all stages of investigation and treatment, i.e. before, during and after the operation. Recently we performed a open heart surgery successfully for a six-years old girl who had suffered from frequent upper respiratory tract infection and mild to moderate exertional dyspnea with congenital heart defects (ventricular septal defect, Kirklin type II, with patent foramen ovale). The operation and postoperative course were very smooth. And now we report this with review of literatures.

심장수술 2,450예와 심장이식

조광현,황윤호,이양행,류지윤 인제대학교 1999 仁濟醫學 Vol.20 No.1S

인제대학교 부산백병원에서는 1985년 10원 개심술이 처음 시행된 이후 1998년 말까지 2,450예의 개심및 비개심 심장수술이 이루어졌는데 이중 개심술이 2,290예, 비개심술은 160예 였다. 개심술 중 선천성이 1,697예(74.1%), 후천성이 573예(25.9%)였으며, 선천성 중에는 비청색증군이 1,548예(91.2%), 청색증군이 149예(8.8%)이었다. 후천성 중에는 심장판막질환이 471예(79.6%)로 가장 많았고, 관상동맥우회수술이 73예(12.1%), 대동맥류수술이 28예(4.7%), 심장종양절제술이 15예(2.5%) 및 심장이식수술이 2예, 기타 4예 등이었다. 특히 1993년부터 심장이식술을 위한 기초 및 동물실험 을 계속하다가 1997년 9월 말기심부전증환자에게 심장이식이 성공적으로 이루어져 개심술의 새로운 장을 열게 되었다. 술후 조기 사망은 선천성 비청색증군에서 30예(1.9%), 청색증군에서는 27예(16.7%), 후천성질환군에서는 26예(4.4%)였고, 전체 수술성공률은 96.5% 로 비교적 좋은 성적을 거두었다. From Sep. 1985 to Dec. 1998, 2,450 cases of heart operation including 2 cases of orthotopic heart transplantaion were performed in the Department of Cardiothoracic Surgery, Inje Universty, Pusan Paik Hospital The results of retrospective clinical review of them were obtained as follows. 1.Open heart surgery cases and non-open heart surgery cases were 2.290 and 160, respectively. Among the open heart cases, congenital cases and acquired cases were 1,697(74.1%) and 593 (25.9%), respectively. 2.Among the congenital cases, acyanotic cases and cyanotic cases were 1,548(91.2%) and 149(8.8%), respectively. The postoperative hospital mortality was 1.9% (30 cases) in the acynotic group and 16.6% (25 cases) in the cyanotic group. 3.Among the acquired cases, valvular heart disease, coronary artery disease, aortic aneurysm, cardiac tumor, heart transplantation and others were 471 (79.6%), 73(12.1%), 28(4.7%), 15(2.5%), 2(0.3%) and 4(0.7%) cases, respectively. 4.Prosthetic valve replacement (457 case, 97.0%) and valve reconstructive surgery(14 cases, 3.0%) were performed for the valvular diseases. Postoperative hospital mortality of valve surgery and coronary artery bypass surgery was 2.8% (13 cases) and 28.6% (6 cases), respectively. 5.28 cases of aortic aneurysm (ascending or arch aneurysm) were operated under cardiopulmonary bypass. The causes of aneurysm were annuloaortic ectasia(9), dissecting aortic aneurysm(16), and non-dissecting chronic aneurysm(8). The early postoperative hospital mortality was 8 cases(28.6%). 6.Two cases of heart transplantation were performed successfully using Shumway method .The total ischemic time of donor heart was 80 minutes and 50 minutes respectively. 7.Minimally invasive surgery (using lower sternal incision) was performed successfully in the cases of mitral and tricuspid valve diseases(10) and congenital septal defects (20). In conclusion, the over all postoperative hospital mortality was not so high as 3.5 %, whereas more proper management for congenital cyanotic group was seemed to be necessary.

Trirutile type 고체촉매를 이용한 olefin의 에폭시화 반응에 관한 연구

조영범,안광현 慶熙大學校 材料科學技術硏究所 2000 材料科學技術硏究論集 Vol.13 No.-

과산화수소를 이용한 알켄의 산화 반응에서 trirutile type 고체 산화물이 효과적으로 촉매 역할하는 것을 관찰할 수 있었다. 특별히 HNbMoO_(6), LiNbMoO_(6) 및 HNbWO_(6)등이 좋은 촉매효과를 보여주었다.

부산 백병원에서 실시한 개심술의 최근 기법

조광현 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.1S

■ Background 3,175 cardiac surgeries were performed in the Pusan Paik Hospital from Sep. 1985 to April 2002, and recently several kinds of current techniques for open heart surgery were adopted with good results. ■ Method Retrospective analysis of the cases and review of the results of operation and usefulness of current techniques such as OFF-Pump CABG. ARCH-FIRST TECHNIQUE. MICRO-WAVE ABLATION, TRANSPLANTATION and MINIMAL INCISION were done. ■ Results ① Among the total cases (3,179), open heart and non-open heart surgery cases were 1,988 and 218, respectively. ② 188 cases of CABG were done. Among them, 20 cases underwent OFF-PUMP coronary artery bypass surgery. 21 cases underwent complete arterial revascularization, and 14 cases underwent both. ③ 75 cases of aortic aneurysm (ascending or arch aneurysm) were operated undercardiopulmonary bypass. The causes of these aneurysm were dissecting aneurysm (45), annuloaortic ectasia(24), non-dissecting chronic aneurysm(5). In the surgery of arch aneurysm. 7 cases underwent ARCH-FIRST TECHNIQUE with good results. ④ In the treatment of atrial fibrillation coupled with valvular heart disease. MICRO-WAVE ABLATION were performed in 18 cases with 83.3 % sinus rhythm recovery. ⑤ Four cases of heart transplantation were performed, using Shumway method, with 3 survivals and one death. ⑥ Minimally invasive incision (using lower sternal incision) was performed successfully in the cases of tricuspid valve diseases(10) and congenital septal defects (50). ■ Conclusion The over all postoperative hospital mortality with open heart surgery was not so high as 3.5 %, whereas more proper management of the all patients with current techniques must be continued.

Air abrasion 기술을 이용한 유치 및 영구치의 수복

조현,이광희,김대업,송인경 大韓小兒齒科學會 2002 大韓小兒齒科學會誌 Vol.29 No.2

Air-abrasion 기술은 입자의 운동에너지를 이용하여 비기계적인 방법으로 치아를 처리하거나 치아표면에 존재하는 착색이나 치석을 제거하는 방법으로서, 환자의 불안이나 불편감을 증가시킬 수 있는 압력, 진동이나 열의 발생이 적고 건전한 치질의 보존이 용이하며 특히 소아환자에서 마취의 필요성을 감소시키고 시술부위에서의 타액오염을 가능성을 줄여주고 건조한 시술부위 유지에 용이하고 수복재의 결합력을 증가시켜주는 등의 여러 장점을 지니고 있어, 복합레진을 위한 와동형성, 열구전색을 위한 치아형성, 산 부식 대신 또는 산부식전 치아표면의 처리, 초기 우식증의 진단 등에 이용될 수 있다. 본 증례는 유중절치의 인접면 우식치료, 유구치의 교합면 우식치료, 제1대구치의 우식치료 및 우식 예방을 air abrasion 기술을 사용하여 수복한 바, 환자의 행동조절 및 시술부위의 완전한 건조가 어려운 소아환자에게 있어 임상적으로 유용하다고 여겨지며 양호한 결과를 얻었기에 보고하는 바이다. Air abrasion technology can prepare enamel and dentin for bonding, similar to etching by acidic gels and solutions. Longer treatment can excavate pit and fissures, preparing the tooth for immediate placement of bonded materials. Although not appropriate for every clinical situation, the air abrasive technology minimizes heat, vibration and bone-conducted noise associated with conventional means of caries removal since the cutting is accomplished by air pressure. Also, patients treated with the air-abrasion technology rarely request anesthesia. Air abrasion technology was more effective in treating early carious lesions and stains compared to lesions where caries had already progressed to produce soft dentin and the strong air stream and noise caused by the evacuation system was a major discomfort to pediatric patients, and the experience and skillfulness of clinician should be required for accurate and proper tooth preparation.

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.