Comparative global immune-related gene profiling of somatic cells, human pluripotent stem cells and their derivatives: implication for human lymphocyte proliferation

Chia-Eng Wu,Chen-Wei Yu,Kai-Wei Chang,Wen-Hsi Chou,Chen-Yu Lu,Elisa Ghelfi,Fang-Chun Wu,Pey-Shynan Jan,Mei-Chi Huang,Patrick Allard,Shau-Ping Lin,Hong-Nerng Ho,Hsin-Fu Chen 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced PSCs (iPSCs), represent potentially unlimited cell sources for clinical applications. Previous studies have suggested that hPSCs may benefit from immune privilege and limited immunogenicity, as reflected by the reduced expression of major histocompatibility complex class-related molecules. Here we investigated the global immune-related gene expression profiles of human ESCs, hiPSCs and somatic cells and identified candidate immune-related genes that may alter their immunogenicity. The expression levels of global immune-related genes were determined by comparing undifferentiated and differentiated stem cells and three types of human somatic cells: dermal papilla cells, ovarian granulosa cells and foreskin fibroblast cells. We identified the differentially expressed genes CD24, GATA3, PROM1, THBS2, LY96, IFIT3, CXCR4, IL1R1, FGFR3, IDO1 and KDR, which overlapped with selected immune-related gene lists. In further analyses, mammalian target of rapamycin complex (mTOR) signaling was investigated in the differentiated stem cells following treatment with rapamycin and lentiviral transduction with specific short-hairpin RNAs. We found that the inhibition of mTOR signal pathways significantly downregulated the immunogenicity of differentiated stem cells. We also tested the immune responses induced in differentiated stem cells by mixed lymphocyte reactions. We found that CD24- and GATA3-deficient differentiated stem cells including neural lineage cells had limited abilities to activate human lymphocytes. By analyzing the transcriptome signature of immune-related genes, we observed a tendency of the hPSCs to differentiate toward an immune cell phenotype. Taken together, these data identify candidate immune-related genes that might constitute valuable targets for clinical applications.

Semantic colorization with internet images

Chia, Alex Yong-Sang,Zhuo, Shaojie,Gupta, Raj Kumar,Tai, Yu-Wing,Cho, Siu-Yeung,Tan, Ping,Lin, Stephen Association for Computing Machinery 2011 ACM transactions on graphics Vol.30 No.6

<P>Colorization of a grayscale photograph often requires considerable effort from the user, either by placing numerous color scribbles over the image to initialize a color propagation algorithm, or by looking for a suitable reference image from which color information can be transferred. Even with this user supplied data, colorized images may appear unnatural as a result of limited user skill or inaccurate transfer of colors. To address these problems, we propose a colorization system that leverages the rich image content on the internet. As input, the user needs only to provide a semantic text label and segmentation cues for major foreground objects in the scene. With this information, images are downloaded from photo sharing websites and filtered to obtain suitable reference images that are reliable for color transfer to the given grayscale photo. Different image colorizations are generated from the various reference images, and a graphical user interface is provided to easily select the desired result. Our experiments and user study demonstrate the greater effectiveness of this system in comparison to previous techniques.</P>

Psilocybin for End-of-Life Anxiety Symptoms: A Systematic Review and Meta-Analysis

Chia-Ling Yu,Fu-Chi Yang,Szu-Nian Yang,Ping-Tao Tseng,Brendon Stubbs,Ta-Chuan Yeh,Chih-Wei Hsu,Dian-Jeng Li,Chih-Sung Liang 대한신경정신의학회 2021 PSYCHIATRY INVESTIGATION Vol.18 No.10

Objective To systematically examine the effectiveness and tolerability of psilocybin for treating end-of-life anxiety symptoms. Methods The Medline, Embase, CENTRAL, and PsycINFO databases were searched up to November 25, 2020. We enrolled clinical trials investigating psilocybin for treating end-of-life anxiety symptoms. Meta-analysis was conducted using random-effects model. Results Overall, five studies were included, revealing that psilocybin was superior to the placebo in treating state anxiety at 1 day (Hedges’ g, -0.70; 95% confidence interval, -1.01 to -0.39) and 2 weeks (-1.03; -1.47 to -0.60) after treatment. Psilocybin was more effective than placebo in treating trait anxiety at 1 day (-0.71; -1.15 to -0.26), 2 weeks (-1.08; -1.80 to -0.36), and 6 months (-0.84; -1.37 to -0.30) after treatment. Psilocybin was associated with transient elevation in systolic (19.00; 13.58-24.41 mm Hg) and diastolic (8.66; 5.18-12.15 mm Hg) blood pressure compared with placebo. The differences between psilocybin and placebo groups with regard to allcause discontinuation, serious adverse events, and heart rates were nonsignificant. Conclusion Psilocybin-assisted therapy could ameliorate end-of-life anxiety symptoms without serious adverse events. Because of the small sample sizes of the included studies and high heterogeneity on long-term outcomes, future randomized controlled trials with large sample sizes are needed.

An Exploratory Study of Cyber Group Development Process

Feng Yang Kuo,Chia Ping Yu 한국경영정보학회 2001 한국경영정보학회 학술대회논문집 Vol.2001 No.-

Programming by Demonstration in Augmented Reality for the Motion Planning of a Three-Axis CNC Dispenser

Chih-Hsing Chu,Yu-Wang Liu,Pai-Chia Li,Liao-Chuan Huang,Yuan-Ping Luh 한국정밀공학회 2020 International Journal of Precision Engineering and Vol.7 No.5

This study presents a programming by demonstration (PbD) interface in augmented reality for motion planning in a three-axis CNC glue dispenser. The interface assists a human planner to effectively determine dispenser motion in a planning task. An augmented reality application prompts the planner with instructive information in a video of real environment while guiding the machine in 3D space to dispense glue on a work part. The purpose involves facilitating the generation of the dispenser’s motion commands by enhancing individual spatial reasoning between the dispenser’s tip and the work part. CAD models are not required prior to the planning task, thus eliminating the use of motion planning software. Test results demonstrate the effectiveness of the proposed interface with respect to accelerating the process of the dispenser PbD. The study shows the practical value of augmented reality as a cost-effective interfacing technology for human–machine collaborations in smart manufacturing.

Where Are Landscape Designers' Spatial Abilities in the Brain? An fMRI Study

Shih-Han Hung,Chia-Yi Huang,Tsung-Ren Huang,Shih-An Tang,Yu-Ping Tsai,Chun-YenChang 인간식물환경학회 2023 인간식물환경학회지 Vol.26 No.5

Background and objective: To effectively understand and communicate their work, landscape designers should possessexcellent spatial abilities. Neurological methods have confirmed that activation of the occipital lobe, parietal cortex, andprefrontal cortex affect the judgment of space; however, few studies have measured spatial abilities in landscape design. This study aimed to identify the potential role of various brain regions during spatial interpretation processes by landscapedesigners, particularly the effect of stimulating the frontal lobe on enhancing design capabilities. Methods: This study tested the spatial abilities of landscape designers when transforming a planar drawing into a sectionaldrawing and the brain regions activated in this process. The subjects were asked to identify the correct option whenmatching given section lines in a planar drawing. The correct answer rate and response time were used to score brainactivation during spatial task processes. A total of 16 valid subjects were divided into high- and low-accuracy groupsaccording to the correct answer rate. Results: The results for the high-accuracy group showed that the left inferior frontal gyrus was activated during spatialdesign tasks. In contrast, the findings for the low-accuracy group revealed that the left middle occipital gyrus was activatedfor processing visual information. Conclusion: The findings suggest that the frontal lobe plays a role in allowing landscape designers to make planar tocross-sectional inferences via mental rotations and categorical spatial relations. The findings offer implications forlandscape designers in stimulating the frontal lobe and enhancing their design capabilities.