Two New Species of Placolecis (Lichenized Ascomycota) from China

( An Cheng Yin ),( Xin Yu Wang ),( Dong Liu ),( Yan Yun Zhang ),( Mei Xia Yang ),( Li Juan Li ),( Li Song Wang ) 한국균학회 2019 Mycobiology Vol.47 No.4

Two new species of the lichen genus Placolecis are discovered in China, namely P. kunmingensis An. C. Yin & Li S. Wang and P. sublaevis An. C. Yin & Li S. Wang. The new combination P. loekoesiana (S.Y. Kondr., Farkas, J.J. Woo & Hur) An. C. Yin is proposed. Placolecis kunmingensis is characterized by having simple, spherical or ellipsoid, hyaline spores, and pear-shaped pycnidia; while P. sublaevis can be distinguished by its thallus forming larger aggregations with slightly flattened lobes at the thallus margin, and urn-shaped pycnidia. Descriptions, a phylogenetic tree and a key are provided for all the known Placolecis species in China.

Inhibition of Microcystis aeruginosa by the Extracellular Substances from an Aeromonas sp

( Yu Mei Liu ),( Ming Jun Chen ),( Meng Hui Wang1 ),( Rui Bao Jia ),( Li Li ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.9

Growth of Microcystis aeruginosa could be inhibited significantly within 24 h by the extracellular substances prepared from Aeromonas sp. strain FM. During the treatment, the concentration of extracellular soluble carbohydrates increased significantly in algal culture. Morphological and ultrastructural changes in M. aeruginosa cells, including breakage of the cell surface, secretion of mucilage, and intracellular disorganization of thylakoids, were observed. HPLC-MS analysis showed that the extracellular substances of Aeromonas sp. strain FM were a mixture of free amino acids, tripeptides, and clavulanate. Among these, the algaelysis effects of lysine and clavulanate were confirmed.

A novel flexible micro-ratchet/ZnO nano-rods surface with rapid recovery icephobic performance

Wang, Manxiang,Yu, Wenbo,Zhang, Yupei,Woo, Jae-Young,Chen, Yu,Wang, Bo,Yun, Yanbin,Liu, Guicheng,Lee, Joong Kee,Wang, Lei Elsevier 2018 Journal of industrial and engineering chemistry Vol.62 No.-

<P><B>Abstract</B></P> <P>In this communication, we present a significant method to fabricate a robust icephobic surface on a flexible polymer substrate. The flexible functional surface is obtained by integrating both soft-lithography and crystal growth methods. Modified by the materials with low surface free energy, the composite surface exhibits a robust superhydrophobicity at not only room temperature but also low temperature. After freezing test, the surface can recover to its original function quickly, which has achieved the level for industrial application, and also performed significant role for enhancing the icephobic theories.</P> <P><B>Highlights</B></P> <P> <UL> <LI> ZnO nano-rods are uniformly planted on the flexible PDMS surface. </LI> <LI> Fabrication of the surface integrates both crystal growth and physical vapor deposition methods. </LI> <LI> The superhydrophobic surface successfully realizes fast-recovery from freezing state. </LI> <LI> In freezing process, the nanorods can hardly cracked due to the flexible basement. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>The liquid droplet can recover to sphere state (Cassie's state) after melting from low temperature (for example, 20°C). ▪</P> <P>[DISPLAY OMISSION]</P>

Metabolism and excretion of novel pulmonary-targeting docetaxel liposome in rabbits

Wang, Jie,Zhang, Li,Wang, Lijuan,Liu, Zhonghong,Yu, Yu The Korean Society of Pharmacology 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.1

Our study aims to determine the metabolism and excretion of novel pulmonary-targeting docetaxel liposome (DTX-LP) using the in vitro and in vivo animal experimental models. The metabolism and excretion of DTX-LP and intravenous DTX (DTX-IN) in New Zealand rabbits were determined with ultra-performance liquid chromatography tandem mass spectrometry. We found DTX-LP and DTX-IN were similarly degraded in vitro by liver homogenates and microsomes, but not metabolized by lung homogenates. Ultra-performance liquid chromatography tandem mass spectrometry identified two shared DTX metabolites. The unconfirmed metabolite $M_{un}$ differed structurally from all DTX metabolites identified to date. DTX-LP likewise had a similar in vivo metabolism to DTX-IN. Conversely, DTX-LP showed significantly diminished excretion in rabbit feces or urine, approximately halving the cumulative excretion rates compared to DTX-IN. Liposomal delivery of DTX did not alter the in vitro or in vivo drug metabolism. Delayed excretion of pulmonary-targeting DTX-LP may greatly enhance the therapeutic efficacy and reduce the systemic toxicity in the chemotherapy of non-small cell lung cancer. The identification of $M_{un}$ may further suggest an alternative species-specific metabolic pathway.

LiFePO₄ quantum-dots composite synthesized by a general microreactor strategy for ultra-high-rate lithium ion batteries

Wang, Bo,Xie, Ying,Liu, Tong,Luo, Hao,Wang, Bin,Wang, Chunhui,Wang, Lei,Wang, Dianlong,Dou, Shixue,Zhou, Yu Elsevier 2017 Nano energy Vol.42 No.-

<P><B>Abstract</B></P> <P>Due to the relatively slow, diffusion-controlled faradaic reaction mechanisms of conventional LiFePO<SUB>4</SUB> (LFP) materials, which is hard to deliver satisfied capacity for high rate applications. In this work, ultrafine LFP quantum dots (LFP-QDs) co-modified by two types of carbonaceous materials - amorphous carbon and graphitized conductive carbon (graphene) have been successfully synthesized through a novel microreactor strategy. Because of the very limited area constructed by the dual-carbon microreactor for the growth of LFP crystal, it's demension was furthest suppressed to a very small level (~ 6.5nm). Such a designed nano-composite possesses a large specific surface area for charge adsorption and abundant active sites for faradaic reactions, as well as ideal kinetic features for both electron and ion transport, and thus exhibits ultra-fast, surface-reaction-controlled lithium storage behavior, mimicking the pseudocapacitive mechanisms for supercapacitor materials, in terms of extraordinary rate capability (78mAhg<SUP>−1</SUP> at 200C) and remarkable cycling stability (~ 99% over 1000 cycles at 20C). On the other side, due to the quasi-2D structure of the synthesized LFP-QDs composite, which can be used as the basic unit to further fabricate free-standing film, aerogel and fiber electrode without the addition of binder and conductive agent for different practical applications. In addition, to deeper understand its electrochemical behavior, a combined experimental and density functional theoretical (DFT) calculation study is also introduced.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A general microreactor strategy has been developed for structure-optimized Li-contained electrode materials. </LI> <LI> Ultrafine LiFePO<SUB>4</SUB> quantum dots are first reported through the designed microreactor strategy. </LI> <LI> The synthesized G/LFP-QDs@C exhibits ultra-fast, surface-reaction-controlled Li storage behavior. </LI> <LI> A combined experimental and DFT calculation study is introduced to reveal the energy storage mechanism of G/LFP-QDs@C. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Ultrafine LiFePO<SUB>4</SUB> quantum dots (~ 6.5nm) co-modified by two types of carbonaceous materials - amorphous carbon and graphitized conductive carbon (graphene) have been successfully synthesized through a novel microreactor strategy, which exhibit ultra-fast, surface-reaction-controlled energy storage behavior, mimicking the pseudocapacitive mechanisms for supercapacitor materials, in terms of excellent rate capability and outstanding cycling stability.</P> <P>[DISPLAY OMISSION]</P>

ZnO Nanorod Array Modified PVDF Membrane with Superhydrophobic Surface for Vacuum Membrane Distillation Application

Wang, Manxiang,Liu, Guicheng,Yu, Hyunjin,Lee, Sang-Hyup,Wang, Lei,Zheng, Jianzhong,Wang, Tao,Yun, Yanbin,Lee, Joong Kee American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.16

<P>The vacuum membrane distillation (VMD) is a promising technology for lots of applications. To solve the membrane fouling and wetting problems, in this paper, a novel ZnO nanorods 1<I>H</I>,1<I>H</I>,2<I>H</I>,2<I>H</I>-perfluorodecyltriethoxysilane (PDTS) modified poly(vinylidene fluoride) (PVDF) membrane with a micro/nanoscale hierarchical structure and a superhydrophobic surface has been prepared and applied to the VMD process for distilling highly salty water, for the first time. Among these, a pyrolysis-adhesion method is created to obtain the ZnO seeds and fasten them on the PVDF substrate firmly. The novel modified membrane shows a stable superhydrophobic surface with a water contact angle of 152°, easy cleaning property, excellent thermal and mechanical stability, because of the Cassie’s state caused by pocketing much air in the hydrophobized ZnO nanorods, the low surface energy of PDTS coating, and the strong adhesion between ZnO nanorods and PVDF membrane, which has built an ideal structure for VMD application. After 8 h VMD of 200 g L<SUP>-1</SUP> NaCl solution, compared to the virgin PVDF membrane, the novel membrane shows a similar permeate flux but a much higher quality permeated liquid because of its unique antifouling and antiwetting caused by the several microns gap between the feed and the membrane. Due to its easy cleaning property, the novel membrane also exhibits an excellent reusability.</P> [FIG OMISSION]</BR>

Transiting Exoplanet Monitoring Project (TEMP). I. Refined System Parameters and Transit Timing Variations of HAT-P-29b

Wang, Songhu,Wang, Xian-Yu,Wang, Yong-Hao,Liu, Hui-Gen,Hinse, Tobias C.,Eastman, Jason,Bayliss, Daniel,Hori, Yasunori,Hu, Shao-Ming,Li, Kai,Liu, Jinzhong,Narita, Norio,Peng, Xiyan,Wittenmyer, R. A.,Wu American Astronomical Society 2018 The Astronomical journal Vol.156 No.4

Dissecting Causal Relationships Between Gut Microbiota, Blood Metabolites, and Stroke: A Mendelian Randomization Study

Wang Qi,Dai Huajie,Hou Tianzhichao,Hou Yanan,Wang Tiange,Lin Hong,Zhao Zhiyun,Li Mian,Zheng Ruizhi,Wang Shuangyuan,Lu Jieli,Xu Yu,Liu Ruixin,Ning Guang,Wang Weiqing,Bi Yufang,Zheng Jie,Xu Min 대한뇌졸중학회 2023 Journal of stroke Vol.25 No.3

Background and Purpose We investigated the causal relationships between the gut microbiota (GM), stroke, and potential metabolite mediators using Mendelian randomization (MR). Methods We leveraged the summary statistics of GM (n=18,340 in the MiBioGen consortium), blood metabolites (n=115,078 in the UK Biobank), and stroke (cases n=60,176 and controls n=1,310,725 in the Global Biobank Meta-Analysis Initiative) from the largest genome-wide association studies to date. We performed bidirectional MR analyses to explore the causal relationships between the GM and stroke, and two mediation analyses, two-step MR and multivariable MR, to discover potential mediating metabolites. Results Ten taxa were causally associated with stroke, and stroke led to changes in 27 taxa. In the two-step MR, <i>Bifidobacteriales</i> order, <i>Bifidobacteriaceae</i> family, <i>Desulfovibrio</i> genus, apolipoprotein A1 (ApoA1), phospholipids in high-density lipoprotein (HDL_PL), and the ratio of apolipoprotein B to ApoA1 (ApoB/ApoA1) were causally associated with stroke (all <i>P</i><0.044). The causal associations between <i>Bifidobacteriales</i> order, <i>Bifidobacteriaceae</i> family and stroke were validated using the weighted median method in an independent cohort. The three GM taxa were all positively associated with ApoA1 and HDL_PL, whereas <i>Desulfovibrio</i> genus was negatively associated with ApoB/ApoA1 (all <i>P</i><0.010). Additionally, the causal associations between the three GM taxa and ApoA1 remained significant after correcting for the false discovery rate (all q-values <0.027). Multivariable MR showed that the associations between <i>Bifidobacteriales</i> order, <i>Bifidobacteriaceae</i> family and stroke were mediated by ApoA1 and HDL_PL, each accounting for 6.5% (<i>P</i>=0.028) and 4.6% (<i>P</i>=0.033); the association between <i>Desulfovibrio</i> genus and stroke was mediated by ApoA1, HDL_PL, and ApoB/ApoA1, with mediated proportions of 7.6% (<i>P</i>=0.019), 4.2% (<i>P</i>=0.035), and 9.1% (<i>P</i>=0.013), respectively. Conclusion The current MR study provides evidence supporting the causal relationships between several specific GM taxa and stroke and potential mediating metabolites.

Targeting neddylation pathway with MLN4924 (Pevonedistat) induces NOXA-dependent apoptosis in renal cell carcinoma

Wang, J.,Wang, S.,Zhang, W.,Wang, X.,Liu, X.,Liu, L.,Li, L.,Liang, Y.,Yu, J.,Jeong, L.S.,Jia, L.,Zhao, H.,Zhang, Y. Academic Press 2017 Biochemical and biophysical research communication Vol. No.

Inhibition of protein neddylation pathway has emerged an attractive anticancer strategy in preclinical studies by using Nedd8-activating enzyme (NAE) inhibitor MLN4924 (Pevonedistat). Previous studies have reported the antitumor activity of MLN4924 mediated by its efficacy on apoptosis, autophagy and senescence. However, whether MLN4924 has any effect on renal carcinoma cells (RCC) remains unexplored. Here we reported that MLN4924 specifically inhibited protein neddylation pathway, leading to statistically significantly suppress the proliferation, survival and migration of RCC cells by inducing G<SUB>2</SUB> cell-cycle arrest, followed by apoptosis in a MLN4924 dose-dependent manner. Further mechanistic study revealed that MLN4924-induced apoptosis was mediated by substantial up-regulation of pro-apoptotic NOXA. These findings highlighted the anticancer effects of the neddylation inhibitors (e.g. MLN4924) for the treatment of RCC.

Evaluation of porcine urine-derived cells as nuclei donor for somatic cell nuclear transfer

Yu-Ting Zhang,Wang Yao,Meng-Jia Chai,Wen-Jing Liu,Yan Liu,Zhong-Hua Liu,Xiao-Gang Weng 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.2

Background: Somatic cell nuclear transfer (SCNT) is used widely in cloning, stem cell research, and regenerative medicine. The type of donor cells is a key factor affecting the SCNT efficiency. Objectives: This study examined whether urine-derived somatic cells could be used as donors for SCNT in pigs. Methods: The viability of cells isolated from urine was assessed using trypan blue and propidium iodide staining. The H3K9me3/H3K27me3 level of the cells was analyzed by immunofluorescence. The in vitro developmental ability of SCNT embryos was evaluated by the blastocyst rate and the expression levels of the core pluripotency factor. Blastocyst cell apoptosis was examined using a terminal deoxynucleotidyl transferase dUTP nick end-labeling assay. The in vivo developmental ability of SCNT embryos was evaluated after embryo transfer. Results: Most sow urine-derived cells were viable and could be cultured and propagated easily. On the other hand, most of the somatic cells isolated from the boar urine exhibited poor cellular activity. The in vitro development efficiency between the embryos produced by SCNT using porcine embryonic fibroblasts (PEFs) and urine-derived cells were similar. Moreover, The H3K9me3 in SCNT embryos produced from sow urine-derived cells and PEFs at the four-cell stage showed similar intensity. The levels of Oct4, Nanog, and Sox2 expression in blastocysts were similar in the two groups. Furthermore, there is a similar apoptotic level of cloned embryos produced by the two types of cells. Finally, the full-term development ability of the cloned embryos was evaluated, and the cloned fetuses from the urine-derived cells showed absorption. Conclusions: Sow urine-derived cells could be used to produce SCNT embryos.