miR-200a Overexpression in Advanced Ovarian Carcinomas as a Prognostic Indicator

Zhu, Cheng-Liang,Gao, Guo-Sheng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

Background: miR-200a expression is frequently altered in numerous cancers. The aim of the present study was to determine the role of microRNA-200a in advanced ovarian carcinomas. Materials and Methods: We measured miR-200a expression in 72 matched normal ovarian tissues and advanced ovarian carcinomas, and also two ovarian carcinoma cell lines (SKOV3 and SKOV3.ip1 - the latter being more invasive and metastatic than the parental SKOV3) by stem-loop real-time RT-PCR based on TaqMan microRNA assay using U6 as a reference. Levels of miR-200a expression were compared by disease stage, tumor grade, histology, and lymph node involvement. To evaluate the role of microRNA-200a, cell proliferation and invasion of SKOV-3 and SKOV-3.ip1 were analyzed with miR-200a inhibitor/mimic transfected cells. Results: Of 72 paired samples, 65 cancer tissues overexpressed microRNA-200a greater than two fold in comparison with matched normal epithelium. Specifically, patients with lymph node metastasis showed significant elevation. The level correlated with clinicopathological features, including high tumor grade, late disease stage, most notably with lymph node metastasis, but not with tumor histology. In addition, SKOV-3.ip1 cells also overexpressed miR-200a compared with SKOV-3, and miR-200a inhibitor transfected SKOV-3.ip1 cells showed significant reduction in cellular proliferation and invasion, while a miR-200a mimic stimulated the opposite behavior. Conclusions: We provide definitive evidence that miR-200a is up-regulated in a significant proportion of advanced ovarian carcinomas, and that elevated miR-200a expression facilitates tumor progression. Our findings support the notion that miR-200a is an onco-microRNA for ovarian cancer, and elevation is a useful potential diagnostic indicator. This study also provides a solid basis for further functional analysis of miR-200a in advanced ovarian cancer.

Potential Serum Markers for Monitoring the Progression of Hepatitis B Virus-Associated Chronic Hepatic Lesions to Liver Cirrhosis

( Cheng Wu ),( Lijie Liu ),( Peng Zhao ),( Dan Tang ),( Dingkang Yao ),( Liang Zhu ),( Zhiqiang Wang ) 대한소화기학회 2015 Gut and Liver Vol.9 No.5

Background/Aims: To screen for serum protein/peptide biomarkers of hepatitis B virus (HBV)-associated chronic hepatic lesions in an attempt to profile the progression of HBV-associated chronic hepatic lesions using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) techniques. Methods: Using SELDI-TOF MS, serum protein/peptide profiles on the CM10 ProteinChip arrays were obtained from a training group including 26 HBV-associated hepatocellular carcinoma patients with liver cirrhosis (LC), 30 HBV-associated LC patients, 85 patients at different stages of liver fibrosis, and 30 asymptomatic HBV carriers. The most valuable SELDI peak for predicting the progression to LC in HBV-infected patients was identified. Results: A SELDI peak of M/Z 5805 with value for predicting LC in HBV-infected patients was found and was identified as a peptide of the C-terminal fraction of the fibrinogen a-chain precursor, isoform 1. Conclusions: The peptide of the C-terminal fraction of the fibrinogen α-chain precursor, isoform 1 with M/Z 5805, may be a serological biomarker for progression to LC in HBV-infected patients. (Gut Liver 2015;9:665-671)

Study on Simulation Analysis of Reduce Speed Effect for Wind Nets in Front of Crop

( Cheng-chang Lien ),( Zhu-yun Yeh ),( Wei-ling Sun ),( Jeng-liang Lin ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

Due to frequent strong winds from its geographical location in Taiwan, which caused serious damage to vegetables and fruit trees, leading to a large loss of agricultural production. Wind nets can be used to reduce the wind speed in front of fruits and vegetables in order to reduce the impact of wind speed. The purpose of this study is to use Computational Fluid Dynamics(CFD), CFD simulation Software was used to simulate wind tunnel experiments with wind nets before fruits and vegetables to understand the wind tunnel with different ventilation rates under certain wind speed 40 m/s, to simulate the change of wind speed and the distribution of flow field behind the wind nets, Analyze and analyze the change of wind field and the effect of reducing the wind. The size of the wind net was 12m^*6m. According to the CFD simulation results, when the strong wind blows through the wind nets, a negative pressure zone will be generated at a horizontal distance of 0h to 10h in the windshield leeway, and the height of the negative pressure zone will gradually decrease as the horizontal distance increases. The height of the negative pressure zone also decreases with the increase of the ventilation rate of the windbreak. In addition, the analysis results show that the ventilation rate of 40% of the windbreak, the negative pressure vortex gradually disappear, and have the best effect of reducing the wind.

Evident α/γ Interface Sliding in Fully Lamellar TiAl Alloy During Hot Deformation: Metallurgical Observation and Mesoscopic Modelling

Liang Cheng,Fengming Qiang,Bin Zhu,Jinshan Li 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.11

Prominent interface sliding was noted in a fully lamellar Ti–43.5Al–8Nb–0.2W–0.2B alloy deformed in (α + γ) phaseregion with low strain rate, which is evidenced by the drastic α/γ lamellar offsets at the kink boundaries. Due to the effectiveaccommodation of interface sliding, there were only a few substructures produced at the kink bands in spite of the highstrain localization. By using the transmission electron microscopy, the interface sliding was demonstrated to be caused by theglide of pre-existing interfacial dislocations. Based on the metallurgical observations and kinetics analysis, two mesoscopicmodels for interface sliding were established corresponding to two alternative rate-controlling steps, i.e., intra-lamellardislocation climb-controlled or interfacial dislocation glide-controlled. Both of them predicted a Newtonian viscous shearbehavior but the latter was manifested to be more plausible to account for the interface sliding kinetics. In addition, the reliabilityof the model was discussed in detail, as well as the role of interface sliding during hot deformation. The developedmesoscopic model can be readily implemented into the crystal plasticity finite element method for a better understanding ofthe synergistic effects of the individual processes on the high temperature plastic flow of lamellar colonies in TiAl alloys.

DEMO: Deep MR Parametric Mapping with Unsupervised Multi-Tasking Framework

Jing Cheng,Yuanyuan Liu,Yanjie Zhu,Dong Liang 대한자기공명의과학회 2021 Investigative Magnetic Resonance Imaging Vol.25 No.4

Compressed sensing (CS) has been investigated in magnetic resonance (MR) parametric mapping to reduce scan time. However, the relatively long reconstruction time restricts its widespread applications in the clinic. Recently, deep learningbased methods have shown great potential in accelerating reconstruction time and improving imaging quality in fast MR imaging, although their adaptation to parametric mapping is still in an early stage. In this paper, we proposed a novel deep learningbased framework DEMO for fast and robust MR parametric mapping. Different from current deep learning-based methods, DEMO trains the network in an unsupervised way, which is more practical given that it is difficult to acquire large fully sampled training data of parametric-weighted images. Specifically, a CS-based loss function is used in DEMO to avoid the necessity of using fully sampled k-space data as the label, thus making it an unsupervised learning approach. DEMO reconstructs parametric weighted images and generates a parametric map simultaneously by unrolling an interaction approach in conventional fast MR parametric mapping, which enables multi-tasking learning. Experimental results showed promising performance of the proposed DEMO framework in quantitative MR T1ρ mapping.

An improved horizontally reversible plow design based on virtual assembly semantics and constraint

Lin Zhu,Shuang-Shuang Peng,Yin-Yin Qi,Xi Cheng,Wen-Feng Zhang,Liang-Yuan Xu,De-Quan Zhu 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.1

There exit model errors in the constructed Horizontally reversible plough (HRP) structure through Interference check technique (ICT). This is basically due to missing parts and geometric interference and, hence, has significantly adverse effects in improving HRP. In thispaper an improved design to refine the three dimensional (3D) model of HRP is implemented by using Virtual assembly technology(VAT). To achieve this, the authors propose a combined virtual assembly semantics and constraint for assembly planning and simulationof HRP in the commercial software, e.g., SolidWorks. First, the assembly planning of HRP was captured with a semantics-based model,including spatial position, assembly orientation, type and parameters of the semantic entities; secondly, the assembly simulation of HRPwas performed by using constraint-based VAT, e.g. component grasping, moving and releasing; finally, the obtained HRP model wasverified through ICT again. The results demonstrate that not any model error exists in the refined 3D HRP model any longer and thatsemantics and constrain based VAT can support the interactive operation more effectively and accurately than those with geometric constraints. This technique was previously used for HRP model. Based on the refined 3D HRP model, the design improvement of Remotecylinder base (RCB), an important component of HRP, will be forthcoming in a future paper.

8q24 rs4242382 Polymorphism is a Risk Factor for Prostate Cancer among Multi-Ethnic Populations: Evidence from Clinical Detection in China and a Meta-analysis

Zhao, Cheng-Xiao,Liu, Ming,Xu, Yong,Yang, Kuo,Wei, Dong,Shi, Xiao-Hong,Yang, Fan,Zhang, Yao-Guang,Wang, Xin,Liang, Si-Ying,Zhao, Fan,Zhang, Yu-Rong,Wang, Na-Na,Chen, Xin,Sun, Liang,Zhu, Xiao-Quan,Yuan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Background: Evidence supporting an association between the 8q24 rs4242382-A polymorphism and prostate cancer (PCa) risk has been reported in North American and Europe populations, though data from Asian populations remain limited. We therefore investigated this association by clinical detection in China, and meta-analysis in Asian, Caucasian and African-American populations. Materials and Methods: Blood samples and clinical information were collected from ethnically Chinese men from Northern China with histologically-confirmed PCa (n=335) and from age-matched normal controls (n=347). The 8q24 (rs4242382) gene polymorphism was genotyped by polymerase chain reaction-high-resolution melting analysis. We initially analyzed the associations between the risk allele and PCa and clinical covariates. A meta-analysis was then performed using genotyping data from a total of 1,793 PCa cases and 1,864 controls from our study and previously published studies in American and European populations, to determine the association between PCa and risk genotype. Results: The incidence of the risk allele was higher in PCa cases than controls (0.222 vs 0.140, $P=7.3{\times}10^{-5}$), suggesting that the 8q24 rs4242382-A polymorphism was associated with PCa risk in Chinese men. The genotypes in subjects were in accordance with a dominant genetic model (ORadj=2.03, 95%CI: 1.42-2.91, $Padj=1.1{\times}10^{-4}$). Presence of the risk allele rs4242382-A at 8q24 was also associated with clinical covariates including age at diagnosis ${\geq}65$ years, prostate specific antigen >10 ng/ml, Gleason score <8, tumor stage and aggressive PCa, compared with the non-risk genotype ($P=4.6{\times}10^{-5}-3.0{\times}10^{-2}$). Meta-analysis confirmed the association between 8q24 rs4242382-A polymorphism and PCa risk (OR=1.62, 95%CI: 1.39-1.88, $P=1.0{\times}10^{-5}$) across Asian, Caucasian and African American populations. Conclusions: The replicated data suggest that the 8q24 rs4242382-A variation might be associated with increased PCa susceptibility in Asian, Caucasian and African American populations. These results imply that this polymorphism may be a useful risk biomarker for PCa in multi-ethnic populations.

A Novel Endo-Polygalacturonase from Penicillium oxalicum: Gene Cloning, Heterologous Expression and Its Use in Acidic Fruit Juice Extraction

( Bo Lu ),( Liang Xian ),( Jing Zhu ),( Yunyi Wei ),( Chengwei Yang ),( Zhong Cheng ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.4

An endo-polygalacturonase (endo-PGase) exhibiting excellent performance during acidic fruit juice production would be highly attractive to the fruit juice industry. However, candidate endo-PGases for this purpose have rarely been reported. In this study, we expressed a gene from Penicillium oxalicum in Pichia pastoris. The recombinant enzyme PoxaEnPG28C had an optimal enzyme activity at pH 4.5 and 45℃ and was stable at pH 3.0-6.5 and < 45℃. The enzyme had a specific activity of 4,377.65 ± 55.37 U/mg towards polygalacturonic acid, and the K_m and V_max values of PoxaEnPG28C were calculated as 1.64 g/l and 6127.45 U/mg, respectively. PoxaEnPG28C increased the light transmittance of orange, lemon, strawberry and hawthorn juice by 13.9 ± 0.3%, 29.4 ± 3.8%, 95.7 ± 10.2% and 79.8 ± 1.7%, respectively; it reduced the viscosity of the same juices by 25.7 ± 1.6%, 52.0 ± 4.5%, 48.2 ± 0.7% and 80.5 ± 2.3%, respectively, and it increased the yield of the juices by 24.5 ± 0.7%, 12.7 ± 2.2%, 48.5 ± 4.2% and 104.5 ± 6.4%, respectively. Thus, PoxaEnPG28C could be considered an excellent candidate enzyme for acidic fruit juice production. Remarkably, fruit juice production using hawthorn as an material was reported for the first time.

Characteristics and mechanism of Pb(II) adsorption/desorption on GO/ r-GO under sulfide-reducing conditions

Jianfeng Zhang,Xiaodan Xie,Cheng Liang,Weihuang Zhu,Xiaoguang Meng 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.73 No.-

Compared with graphene oxide (GO), r-GO (the reduced form) has a lower adsorption capacity for heavymetal ions and, therefore, generates concern over the release of adsorbed contaminants when GOadsorbent is discharged into a reducing environment. This study reveals that the maximum adsorptioncapacity of Pb(II) on GO and r-GO was 937.65 and 92.99 mg g 1 respectively. GO was reduced to r-GO bysulfide, causing 2.59–6.46% of the adsorbed Pb(II) to be released and was stably dispersed as a Pb(II)-oxidative debris (OD) complex. Our results provide valuable information about heavy metaltransportation in environments containing GO under different redox conditions.

Konjac Glucomannan Derived Carbon Aerogels for Multifunctional Applications

Jie Lian,Jiwei Li,Liang Wang,Ru Cheng,Xiuquan Tian,Xue Li,Jian Zhou,Tao Duan,Wenkun Zhu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.10

Environmental and energy issues have always been a hot topic of global research. Oil leakage has caused great damage to the environment, affecting a wide area and it is difficult to clean up. In most cases, carbon-based adsorbents are typically utilized to remove oil spills because of their economic benefits and high adsorbent efficiency. At the same time, its excellent material properties can also be used for the preparation of supercapacitors. In this paper, the carbon aerogels were prepared by the one-step method. The prepared materials endowed a 3D network structure with a huge number of micropores and mesoporous, and the material is light-weight, stable, hydrophobic and has affinity for oil (17.02 g/g) to the KGM carbon aerogel. Through the physicchemical characterization, the KGM carbon aerogel shows specific surface area is 689m2/g, high water contact angle (136.64°) and excellent reusability (more than 15 cycle times). In addition, we also discussed the electrochemical properties of the material and obtained the specific electrical capacity of 139 F/g under the condition of 1 A/g.