Synthesis, Application, and Recovery of Alkali-Clearable Disperse Dyes Containing Azo Pyridone Structure

Liang Jin,Tao Qian,Ren-Liang Wang,Tao Zhu,Wei-Guo Chen,Hua Jiang,Zhi-Hua Cui 한국섬유공학회 2022 Fibers and polymers Vol.23 No.4

Carboxylic acid ester disperse dyes containing an azo pyridone structure have a high molar extinction coefficient,excellent washing color fastness, and simple synthetic methods. Dyes were readily synthesized through a conventional diazocoupling reaction between the reactant ethyl p-aminobenzoate and a coupling component containing an azo pyridonestructure. Subsequently, the molecular structures of the target products were characterized by FTIR, mass spectrometry,1H NMR, 13C NMR, UV-vis absorption spectroscopy, and elemental analysis. The synthesized dyes were used to dyepoly(ethylene terephthalate) fabric, and their washing and rubbing color fastness properties were tested and compared underdifferent post-treatment methods. Moreover, under the condition of alkali clearing, the relationships among different alkaliconcentrations and clearing effects were studied to determine the optimal alkali clearing process conditions for dyed fabrics,and the minimum alkali consumption required to obtain the best results was determined. It was found that carboxylic esterdisperse dyes containing an azo pyridone structure showed good dyeing performance and alkali-clearable properties onpolyester fibers. In addition, in hydrolyzate recovery, BaCl2 (salting out) can be used instead of acidification to recover thedye hydrolyzate, which has good environmental benefits.

Potential Biomarker of L-type Amino Acid Transporter 1 in Breast Cancer Progression

Liang, Zhongxing,Cho, Heidi T.,Williams, Larry,Zhu, Aizhi,Liang, Ke,Huang, Ke,Wu, Hui,Jiang, Chunsu,Hong, Samuel,Crowe, Ronald,Goodman, Mark M.,Shim, Hyun-Suk The Korea Society of Nuclear Medicine 2011 핵의학 분자영상 Vol.45 No.2

Purpose L-type amino acid transporter 1 (LAT1) is essential for the transport of large neutral amino acids. However, its role in breast cancer growth remains largely unknown. The purpose of the study is to investigate whether LAT1 is a potential biomarker for the diagnosis and treatment of breast cancer. Methods LAT1 mRNA and protein levels in breast cancer cell lines and tissues were analyzed. In addition, the effects of targeting LAT1 for the inhibition of breast cancer cell tumorigenesis were assessed with soft agar assay. The imaging of xenograft with anti-1-amino-3-[$^{18}F$]fluorocyclobutane-1-carboxylic acid (anti-[$^{18}F$]FACBC) PET was assessed for its diagnostic biomarker potential. Results Normal breast tissue or low malignant cell lines expressed low levels of LAT1 mRNA and protein, while highly malignant cancer cell lines and high-grade breast cancer tissue expressed high levels of LAT1. In addition, higher expression levels of LAT1 in breast cancer tissues were consistent with advanced-stage breast cancer. Furthermore, the blockade of LAT1 with its inhibitor, 2-aminobicyclo[ 2.2.1]heptane-2-carboxylic acid (BCH), or the knockdown of LAT1 with siRNA, inhibited proliferation and tumorigenesis of breast cancer cells. A leucine analog, anti-[$^{18}F$]FACBC, has been demonstrated to be an excellent PET tracer for the non-invasive imaging of malignant breast cancer using an orthotopic animal model. Conclusions The overexpression of LAT1 is required for the progression of breast cancer. LAT1 represents a potential biomarker for therapy and diagnosis of breast cancer. Anti-[$^{18}F$]FACBC that correlates with LAT1 function is a potential PET tracer for malignant breast tumor imaging.

miR-200a Overexpression in Advanced Ovarian Carcinomas as a Prognostic Indicator

Zhu, Cheng-Liang,Gao, Guo-Sheng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

Background: miR-200a expression is frequently altered in numerous cancers. The aim of the present study was to determine the role of microRNA-200a in advanced ovarian carcinomas. Materials and Methods: We measured miR-200a expression in 72 matched normal ovarian tissues and advanced ovarian carcinomas, and also two ovarian carcinoma cell lines (SKOV3 and SKOV3.ip1 - the latter being more invasive and metastatic than the parental SKOV3) by stem-loop real-time RT-PCR based on TaqMan microRNA assay using U6 as a reference. Levels of miR-200a expression were compared by disease stage, tumor grade, histology, and lymph node involvement. To evaluate the role of microRNA-200a, cell proliferation and invasion of SKOV-3 and SKOV-3.ip1 were analyzed with miR-200a inhibitor/mimic transfected cells. Results: Of 72 paired samples, 65 cancer tissues overexpressed microRNA-200a greater than two fold in comparison with matched normal epithelium. Specifically, patients with lymph node metastasis showed significant elevation. The level correlated with clinicopathological features, including high tumor grade, late disease stage, most notably with lymph node metastasis, but not with tumor histology. In addition, SKOV-3.ip1 cells also overexpressed miR-200a compared with SKOV-3, and miR-200a inhibitor transfected SKOV-3.ip1 cells showed significant reduction in cellular proliferation and invasion, while a miR-200a mimic stimulated the opposite behavior. Conclusions: We provide definitive evidence that miR-200a is up-regulated in a significant proportion of advanced ovarian carcinomas, and that elevated miR-200a expression facilitates tumor progression. Our findings support the notion that miR-200a is an onco-microRNA for ovarian cancer, and elevation is a useful potential diagnostic indicator. This study also provides a solid basis for further functional analysis of miR-200a in advanced ovarian cancer.

MiR-454 Prompts Cell Proliferation of Human Colorectal Cancer Cells by Repressing CYLD Expression

Liang, Hong-Liang,Hu, Ai-Ping,Li, Sen-Lin,Xie, Jia-Ping,Ma, Qing-Zhu,Liu, Ji-Yong Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.6

Previous studies have shown that miR-454 plays an important role in a variety of biological processes in various human cancer cells. However, the underlying mechanisms of this microRNA in colorectal cancer (CRC) cells remain largely unknown. In the present study, we investigated the miR-454 role in CRC cell proliferation. We found that miR-454 expression is markedly upregulated in CRC tissues and CRC cells compared with the matched tumor adjacent tissues and the FHC normal colonic cell line. Ectopic expression of miR-454 promoted the proliferation and anchorage-independent growth of CRC cells, whereas inhibition of miR-454 reduced this effect. Bioinformatics analysis further revealed cylindromatosis (CYLD), a putative tumor suppressor as a potential target of miR-454. Data from luciferase reporter assays showed that miR-454 directly binds to the 3'-untranslated region (3'-UTR) of CYLD mRNA and repressed expression at both transcriptional and translational levels. In functional assays, CYLD-silenced in miR-454-in-transfected SW480 cells have positive effect to promote cell proliferation, suggesting that direct CYLD downregulation is required for miR-454-induced CRC cell proliferation. In sum, our data provide compelling evidence that miR-454 functions as an onco-miRNA, playing a crucial role in the promoting cell proliferation in CRC, and its oncogenic effect is mediated chiefly through direct suppression of CYLD expression.

Impact of a Clinical Pathway on Hospital Costs, Length of Stay and Early Outcomes after Hepatectomy for Hepatocellular Carcinoma

Zhu, Liang,Li, Jun,Li, Xiao-Kang,Feng, Jun-Qiang,Gao, Jian-Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

Background: A clinical pathway (CP) can standardize and improve perioperative care for a number of interventions. In hepatic surgery, however, pertinent evidence is very limited. This study was conducted to implement a CP for hepatocellular carcinoma (HCC) patients undergoing hepatectomy, and to evaluate its effects on hospital costs, length of hospital stay (LOHS) and early clinical outcomes. Materials and Methods: Medical records for HCC patients undergoing hepatectomy were retrospectively reviewed before implementation of a CP (the non-CP group) from March 2012 to August 2012. This information was compared with the data collected prospectively from patients after implementation of the CP (the CP group) between September 2012 and April 2013. Hospital costs, LOHS and early clinical outcomes were evaluated and compared between groups. Results: There were no significant differences in terms of patient clinical characteristics between the two groups. For clinical outcome measures, no significant differences were found in postoperative complications, mortality and readmission rate. The hospital costs were significantly reduced from 24,844 RMB in the non-CP group to 19,761 RMB in the CP group (p<0.01). In addition, patients of the CP group also had shorter LOHS compared with the non-CP group (8.3 versus 12.3 days, p<0.001). Conclusions: The CP proved to be an effective approach to minimize hospital costs and LOHS with hepatectomy for HCC without compromising patient care.

The Design of Fingerprint Identification System based on Improved Binarization Algorithm

Zhu Heng-jun,Guo Ying,Zhang nan-nan,Liang Hong 보안공학연구지원센터 2014 International Journal of Security and Its Applicat Vol.8 No.6

Effect of temperature on the development of Echinothrips americanus Morgan (Thysanoptera: Thripidae) with special reference to the number of generations

Liang Zhu,Ze-Hua Wang,Ya-Jun Gong,Li-Jun Cao,Shu-Jun Wei 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.4

The thrips Echinothrips americanus Morgan (Thysanoptera: Thripidae) was recently reported in China. The effect of temperature on its development was investigated and the potential area of further dispersal was predicted by estimation of its generations in China. First instar larvae developed to adults on green peppers Capsicum anuum at all constant temperatures (16, 19, 22, 25, 28, 31 and 34 °C) except for 34 °C, at which they developed only as far as the second instar. The percentage surviving of immature stages were not significantly different at the other six temperatures (range 32.10%–52.72%). The developmental times from egg to adult ranged from 11.68 to 45.13 days and were negatively correlated with temperature. There were significant differences between male and female immature stages in their developmental time at 22 °C and 25 °C. Regression analysis indicated that both the Lactin and linear models could describe the relationship between developmental rate and temperature for immature stages but a slightly higher coefficient of determination was obtained with the Lactin model. The high-temperature threshold and the optimum temperature for development were 32.04 °C and 31.79 °C, respectively, estimated by the Lactin model. The low-temperature threshold for development was 11.35 °C and thermal constant was 229.76 degree-days for immature stages, estimated by the linear model. The models predicted that E. americanus could have produced 8–10 generations per year under field conditions in Beijing (N39°56′, E116°17′) and Yangling (N34°12′, E108°7′) from 2002 to 2012. The potential risk posed by E. americanus to crops in different regions of China was discussed.

Adsorption of copper on iminodisuccinic acid modified attapulgite: characterization and mechanism

Qiuzi Zhu,Jianzhong Zhu,Meng Su,Liang Liu,Cunshi Wang,Dongliang Ji,Wangjun Bai,Wentao Shen 대한환경공학회 2023 Environmental Engineering Research Vol.28 No.4

Heavy-metal ions are common pollutants in wastewater and are thus attracting considerable attention. Herein, an eco-friendly biodegradable adsorbent, iminodisuccinic acid (IDS) modified attapulgite (ATP) is prepared by graft-polymerization to reduce Cu(II) in water, referred as IDS-ATP. The equilibrium adsorption capacity of IDS-ATP for Cu(II) is increased by 329.5% and 272% compared with raw ATP and non-degradable chelator ethylenediaminetetraacetic acid-modified ATP (EDTA-ATP), respectively. Moreover, the adsorption capacities for Cu(II) in combined system increased by 186% compared with in single system. The structure and surface properties of IDS-ATP are characterized, demonstrating that the IDS moieties are anchored on the surface of ATP without structural damage. In the aqueous Cu(II) (64 mg /L), the best adsorption pH is 5.0, the best dosage is 800 mg/L, and the adsorption equilibrium time is 4 h. The adsorption of IDS-ATP is chemical adsorption and regenerated adsorbent still exhibits high adsorption capacity. The adsorption mechanism includes the coordination of amino groups with Cu(II), the chelation of -COOH on heavy metals (HMs), and the ion exchange. Taking Cu(II) as an example to study the process of IDS-ATP in water, it is beneficial to apply this degradable material to reduce the other HMs.

PMN-PT Single Crystal for Endoscopic Ultrasound 2D Array Application

Yuhang Zhu,Huageng Liang,Benpeng Zhu,Dan Zhou,Xiaofei Yang 대한금속·재료학회 2017 ELECTRONIC MATERIALS LETTERS Vol.13 No.2

Based on lead magnesium niobate-lead titanate single crystal, a 24 × 24row-column addressing endoscopic two-dimensional array has beensuccessfully fabricated using novel flanged electrodes and “semi-kerf”technologies. Each row/column array element was measured to have anelectromechanical coupling coefficient of 0.81, a center frequency of5MHz, and a fractional bandwidth of approximately 88% at −6 dB. Ofparticular significance was that the lead magnesium niobate-lead titanateelement exhibits much higher sensitivity compared with lead zirconatetitanate-based 2D arrays with similar operational frequency and elementarea. According to the Field II simulated results, although the obtainedbeamwidth at −6 dB was a little inferior to that of the fully sampled 24 × 24two-dimensional array, it is believed that the beamwidth can be improved byappropriately increasing the element number. These results demonstratedthat the lead magnesium niobate-lead titanate single-crystal 2D array is apromising candidate for real-time three-dimensional endoscopic ultrasoundimaging.

Improved Mechanical Properties of Friction Stir Welded 1500 MPa Martensitic Steel Joints with Additional Water Cooling

Shuhao Zhu,Pengcheng Zhu,Binbin Wang,Liang Zhang,Yufeng Sun,Shaokang Guan 대한금속·재료학회 2024 METALS AND MATERIALS International Vol.30 No.1

This study proposes a method to improve the mechanical strength of martensitic welded joints by minimizing the effect ofmartensitic tempering. The high-strength martensitic steel plate exhibiting a tensile strength of 1500 MPa was successfullyjoined in this study utilizing additional water-cooling by friction stir welding (FSW) technique. Concurrently, conventionalair-cooled welded joints were produced utilizing identical welding parameters for comparison. Notwithstanding the macrostructureof both FSW joints was similar, the air-cooled joint contained a larger stir zone (SZ) and wider heat-affectedzone (HAZ). The air-cooled joint SZ exhibited a microstructure featuring coarse martensite, while the water-cooled jointSZ displayed a microstructure consisting of fine martensite. The degree of self-tempering of the martensite in the HAZ wassignificantly reduced, together with fewer and finer carbide particles than in the HAZ of air-cooled joints, by applying watercooling. The tensile test results indicated that the water-cooled FSW joint showed a significant improvement (23.6%) inultimate tensile strength compared to its air-cooled counterparts.