자간전증에서 자궁정맥혈장 , 제대정맥혈장 및 말초정맥혈장내의 산화질소농도

박훈(Hoon Park),김윤하(Yoon Ha Kim),이종은(Jong Un Lee),최기오(Kee Oh Chay),김혜정(Hye Jung Kim),정광필(Kwang Pil Jeong),김석모(Seok Mo Kim),송태복(Tae Bok Song),변지수(Ji Soo Byun) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.2

목적 : 자간전증 환자에서는 혈관내피의 강력한 혈관이완물질인 산화질소 (nitric oxide) 생성장애로 인해 혈관확장의 유지에 이상이 초래된다. 본 연구는 자궁정맥혈장, 제대정맥혈장 및 말초정맥혈장내의 산화질소농도를 측정하여 자간전증 임신군과 정상 임신군 간의 차이를 비교하여 이의 유의성을 알고자 한다. 연구 방법 : 18명의 정상 혈압을 보인 산모와 20명의 자간전증에 이환된 산모를 대상으로 하였다. 각 산모에서 제왕절개술 수술전에 상완정맥에서 혈액을 채취하였고 복벽을 열고 태아를 분만하기 전에 자궁정맥, 태아를 분만하고 태반을 분만하기 직전에 제대정맥에서 혈액을 채취하였다. 채취된 혈액에서 Greiss 반응법을 이용하여 산화질소의 대사산물인 nitrite의 농도를 측정하였다. 결과 : 제대정맥혈장내의 산화질소농도는 자간전증 산모군에서 46.53±22.01 M/L, 정상 산모군에서 17.51 ±7.43 M/L로 자간전증 산모에서 의의있게 증가하였다 (p<0.05). 자궁정맥혈장내의 산화질소농도는 자간전증 산모군에서 51.78±14.19 M/L, 정상 산모군에서 21.23±11.6 M/L로 자간전증 산모에서 의의있게 증가하였다 (p<0.05). 상완정맥혈장내의 산화질소농도는 자간전증 산모군에서 66.41±20.87 M/L, 정상 산모군에서 21.26±9.54 M/L로 자간전증 산모에서 의의있게 증가하였다 (p<0.05). 결론 : 산화질소의 생성은 자궁정맥혈장, 제대정맥혈장 및 말초정맥혈장에서 자간전증 산모군이 정상대조군과 비교하여 더 높았다. 이는 자간전증의 병적효과를 상쇄하기 위한 보상적 상승으로 보인다. Objective : The aim of this study was to measure circulating levels of nitric oxide metabolites (nitrites) in the uteroplacental, fetoplacental, and peripheral circulation in women with normal pregnancy and preeclampsia. Methods : Two groups of pregnant women were included : 20 patients with preeclampsia and 18 normotensive women. At cesarean, blood samples were taken from the antecubital vein and uterine vein draining the placental site before delivery of the baby, and the umbilical vein after delivery of the baby. Plasma nitric oxide concentrations were determined with the Griess reaction by measuring combined oxidation products of nitric oxide, plasma nitrite and nitrate after reduction with nitrate reductase. Results : Significantly higher serum nitrite concentrations were found in umbilical (46.53±22.01 vs. 17.51±7.43 M/L, p<0.05), uterine (51.78±14.19 vs. 21.23±11.6 M/L, p<0.05) and antecubital (66.41±20.87 vs. 21.26±9.54 M/L, p<0.05) venous plasma in the preeclamptic group compared with the control group. Conclusion : We observe higher levels of nitirc oxide metabolites in the uteroplacental, fetoplacental, and peripheral circulation in women with preeclampsia than in normal pregnancy. These results support the hypothesis that increased nitric oxide production may be a compensatory response to improve blood flow and offset the pathologic effects of preeclampsia.

GO-77 : Overexpression of Glucose Transporter-1 (GLUT-1) Predicts Poor Prognosis in Epithelial Ovarian Cancer

( Doo Byung Chay ),( Han Byoul Cho ),( Min Young Chang ),( Chung Won Lee ),( Jae Hoon Kim ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.99 No.-

To analyzed the expression of GLUT-1 in epithelial ovarian cancer (EOC) and to evaluate its clinical significance. Illumina microarray was used to identify differentially expressed genes in 3 EOC cells. To validate the microarray data, mRNA and protein level of GLUT-1 was examined by real-time PCR and immunohistochemistry (IHC) and compared with clinicopathological parameters including survival data. GLUT-1 had an EOC/normal cells ratio of 5.51 based on microarray. PCR and immunohistochemistry demonstrated that GLUT-1 expression was significantly increased in EOC (p=0.029 and p<0.001, respectively). Particularly, IHC demonstrated that GLUT-1 expression in serous ovarian cancer was significantly higher than non-serous types (p=0.036). On survival analysis, GLUT-1 overexpression (HR = 4.80 [1.02-22.10], p=0.027) and lymph node (LN) metastases (HR = 8.35 [1.05-46.21], p=0.016) conferred a significantly worse overall survival. GLUT-1 expression is remarkably up-regulated in EOC and predicts a poor overall survival.

ER-60 (PDIA3) protein is highly expressed in a newly established serous ovarian cancer cell Line, YDOV-139(초)

채두병 ( Doo Byung Chay ),( Han Byoul Cho ),( Yoon Jin Oh ),( Sun Mi Choi ),( Bo Wook Kim ),( Young Tae Kim ),( Jae Hoon Kim ) 대한산부인과학회 2009 대한산부인과학회 학술대회 Vol.95 No.-

Comparative proteomic analysis of cysteine oxidation in colorectal cancer patients

Yang, Hee-Young,Chay, Kee-Oh,Kwon, Joseph,Kwon, Sang-Oh,Park, Young-Kyu,Lee, Tae-Hoon Springer-Verlag 2013 Molecules and cells Vol.35 No.6

<P>Oxidative stress promotes damage to cellular proteins, lipids, membranes and DNA, and plays a key role in the development of cancer. Reactive oxygen species disrupt redox homeostasis and promote tumor formation by initiating aberrant activation of signaling pathways that lead to tumorigenesis. We used shotgun proteomics to identify proteins containing oxidation-sensitive cysteines in tissue specimens from colorectal cancer patients. We then compared the patterns of cysteine oxidation in the membrane fractions between the tumor and non-tumor tissues. Using nano-UPLC-MSE proteomics, we identified 31 proteins containing 37 oxidation-sensitive cysteines. These proteins were observed with IAM-binding cysteines in non-tumoral region more than tumoral region of CRC patients. Then using the Ingenuity pathway program, we evaluated the cellular canonical networks connecting those proteins. Within the networks, proteins with multiple connections were related with organ morphology, cellular metabolism, and various disorders. We have thus identified networks of proteins whose redox status is altered by oxidative stress, perhaps leading to changes in cellular functionality that promotes tumorigenesis.</P>

Factors associated with prediction of residual lesion in conization

( Bo Ram Choi ),( Doo Byung Chay ),( Han Byoul Cho ),( Hyun Sun Lim ),( Young Tae Kim ),( Jae Hoon Kim ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.98 No.-

We investigated factors affecting margin involvement, follow-up cytology and high risk human papillomavirus (HPV) testing within 6 months after conization, following surgical treatment after conization as predictors of incomplete excision in conization. One thousand four hundred forty six patients who underwent conization due to diagnose and treat cervical intraepithelial neoplasia (CIN) in Gangnam Serverance and Severance Hospital from January 2008 to December 2011 were included in this retrospective study. We analyzed their clinical and pathologic characteristics. Aging (odd ratio [OR], 1.039; 95% confidence interval [CI], 1.022-1.056), high grade group of preconization cytology(OR, 1.800; 95% CI, 1.333-2.431), preconization high risk HPV testing positivity (OR, 5.215; 95% CI, 1.824-5.215) were statistically significant factors that affect conization margin positivity. The width of specimen in conization was predictive factor for follow-up cytology within 6 months after conization (OR, 0.579; 95% CI, 0.370-0.906). Low grade group of preconization cytology (OR, 0.619; 95% CI, 0.426-0.900), preconizaton HPV testing positivity (OR, 4.726; 95% CI, 2.172-10.28) were significantly different in follow-up high risk HPV testing within 6 months after conization. Aging (OR, 1.066; 95% CI, 1.044-1.090), high grade group of preconization cytology (OR, 2.293; 95% CI, 1.575-3.338), preconization high risk HPV testing (OR, 4.560; 95% CI, 2.158-9.639), local anesthesia (OR, 1.885; 95% CI, 1.136-3.128) were relative to the following surgical treatment after conization. It is important to predict residual lesion after conization that affected by age, parity, preconization cytology and high risk HPV testing, anesthesia. These findings could be used to determine appropriate follow-up protocols.

Prevalence and Seroprevalence of High-Risk Human Papillomavirus Infection :

Kim, Min-A,Oh, Jin-Kyoung,Chay, Doo Byung,Park, Dong Choon,Kim, Seok Mo,Kang, Eun-Suk,Kim, Jae-Hoon,Cho, Chi-Heum,Shin, Hai-Rim,Seo, Kyung Ovid Technologies (Wolters Kluwer) - Lippincott Wi 2010 Obstetrics and gynecology Vol.116 No.4

<P>OBJECTIVE:: To estimate the prevalence and seroprevalence of high-risk human papillomavirus (HPV) in Korean girls and women. METHODS:: We estimated the seroprevalence of HPV subtypes 16 and 18 among 1,094 girls and women aged 9-59 years and the prevalence of genital high-risk HPV among 902 women aged 20-59 years who visited our institution for a medical checkup. Genital high-risk HPV DNA was measured using liquid hybridization and polymerase chain reaction assays. Serum antibodies to HPV subtypes 16 and 18 were measured using a multiplexed competitive luminex technique. RESULTS:: The prevalence of genital high-risk HPV was 12.6% among Korean women aged 20-59 years. It reached a peak of 23.2% at 20-29 years of age, decreasing thereafter but increasing again to 12.4% at 50-59 years of age. Human papillomavirus 56 was the most common subtype followed by HPV 18, HPV 52, and HPV 16. The seroprevalence of HPV subtypes 16 and 18 was 8.7% among Korean girls and women aged 9-59 years. It reached its highest peak of 13.4% at 25-29 years of age and decreased thereafter. It then reached a second peak of 10.9% at 40-49 years of age and plateaued thereafter. The seroprevalences of HPV subtypes 16 and 18 were 7.4% and 2.7%, respectively. In multivariable analysis, the prevalence and seroprevalence of high-risk HPV were correlated only with the number of lifetime sexual partners. CONCLUSION:: High-risk HPV infection is common among Korean women. Our epidemiological data on high-risk HPV infection will help to assess vaccine policy and to establish a baseline for estimating vaccine efficacy. LEVEL OF EVIDENCE:: III.</P>

Application of oncothermia using LabEHY-1 00 inhibits the growth of ovarian and cervical cancer cells

( Wook Yeom Yang ),( Doo Byung Chay ),( Sung Hoon Kim ),( Ha Yeon Shin ),( Eun Ju Lee ),( Jing Yeong Kong ),( Geum Seon Son ),( Ji Hui Choi ),( Jae Hoon Kim ) 대한산부인과학회 2015 대한산부인과학회 학술대회 Vol.101 No.-

Expanding biospecimens from Korean gynecologic cancer bank: Construction of tissue micro arrays and establishment of immortalize cell line

( Geum Seon Sohn ),( Doo Byung Chay ),( Hyun Ja Kwon ),( Wook Yeom Yang ),( Jae Hoon Kim ) 대한산부인과학회 2015 대한산부인과학회 학술대회 Vol.101 No.-

Arthroscopic Meniscectomy for Medial Meniscus Horizontal Cleavage Tears in Patients under Age 45

( Jae Gyoon Kim ),( Seung Yup Lee ),( Suhwoo Chay ),( Hong Chul Lim ),( Ji Hoon Bae ) 대한슬관절학회 2016 대한슬관절학회지 Vol.28 No.3

Purpose: The purpose of this study was to evaluate the demographics, clinical features, and outcomes of arthroscopic partial meniscectomy (APM) for isolated medial meniscus horizontal cleavage tears (MMHCTs) in patients under 45 years of age. Materials and Methods: We retrospectively reviewed 98 patients (100 knees) under 45 years who underwent APM for MMHCTs. Clinical outcomes were assessed using International Knee Document Committee (IKDC) subjective core, Tegner activity scale, visual analog scale (VAS) pain score, and a question on the symptom relief. Results: 79% were male and 70% had no trauma. The mean symptom duration was 10 months. At arthroscopy, a flap tear was identified in 75%. At a mean of 19-month follow-up, the IKDC subjective score, Tegner activity scale, and VAS pain score were significantly improved compared to the preoperative values (p=0.025, p=0.043, and p=0.032, respectively). While 85% were free of symptoms, 15% had persistent pain. No significant differences in outcomes were observed based on the tear type and the presence of flap tears. No progression or development of radiographic degenerative changes was observed in all knees. Conclusions: Demographics of MMHCTs under age 45 showed a male dominance and higher frequency of non-traumatic tears. APM was beneficial to symptomatic HCTs in this cohort during the short-term follow-up. Type of HCTs and combined flap tears did not affect clinical outcomes.

기분조절제로 인한 인지기능 부작용이 동반된 양극성 기분장애 환자의 임상적인 특징: 아시아의 정신작용약물 처방 경향 연구(REAP-BD Survey) 중 국내 자료 활용

신희현,최진희,소형석,최하연,박용천,Chay Hoon Tan,Shih-ku Lin,Naotaka Shinfuku,박선철,김기원 대한신경정신의학회 2020 신경정신의학 Vol.59 No.3

Objectives This study aimed to identify differences between bipolar disorder patients who experienced cognitive side effects and those who did not experience cognitive side effects due to prescription of mood stabilizers. Methods This study analyzed 350 Korean adults who were diagnosed with bipolar disorder and prescribed mood-stabilizing drugs. The patients were divided into two groups—patients who experienced cognitive side effects and those who did not experience cognitive side effects. We also compared the demographic and clinical characteristics between both groups. Results The number of patients with an untreated illness longer than 1 year was higher in the group of patients who experienced cognitive side effects compared to the group of patients who did not experience cognitive side effects. Further, the number of patients with manic symptoms at onset was higher in the group of patients who experienced cognitive side effects compared to the group of patients who did not experience cognitive side effects. In addition, the proportion of patients in remission was higher in the group of patients who experienced cognitive side effects compared to the group of patients who experienced no cognitive side effects. However, there was no significant difference regarding the type of mood stabilizer used between the groups. On the other hand, more people experienced cognitive side effects as the valproic acid dosage increased. Conclusion Our findings suggest that there are clinical and demographic differences between people who experienced cognitive side effects and those who did not experience cognitive side effects due to prescription of mood stabilizers.