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      • KCI등재

        國文法硏究의 諸問題

        申昌淳 한국어학회 2007 한국어학 Vol.35 No.-

        Based on thirty years of my experience studying Korean grammar, I suggest some main topics for the study of Korean grammar; 1) subject and topic 2) 'yeoneo (連語)' and 3) verbal bases and the history of Korean language. To solve the problem of identifying subject and topic, a strongly recommended method is to compare Korean with Japanese, which is one of the languages close to Korean in historical and typological aspects, that is, similar to Korean in grammatical structure. In my definition, the 'yeoneo' is a formation that consists of more than two words but does not form a clause. The best research method could be the one based on the linguistic realism which requires to collect and examine examples containing relevant words focusing on their combinatorial properties and to classify them with regard to research purposes. Considering the theme of verbal bases and the history of Korean language, the proper approach would be the examination of orthographic and phonemic aspects of the records written in the middle Korean, the study of sound of chinese characters in the ancient Korean and the comparison between Korean and other Altaic languages.

      • SCIESCOPUS

        Foodborne Viruses Detected Sporadically in the Fresh Produce and Its Production Environment in South Korea

        Shin, Hansaem,Park, Hyunkyung,Seo, Dong Joo,Jung, Soontag,Yeo, Daseul,Wang, Zhaoqi,Park, Ki Hwan,Choi, Changsun Mary Ann Liebert, Inc. Publishers 2019 Foodborne Pathogens and Disease Vol.16 No.6

        <P> Contamination of fresh vegetables and berries with human enteric viruses is a major cause of food poisoning. The aim of this study was to investigate the prevalence of norovirus GI, norovirus GII, hepatitis A virus (HAV), adenovirus, astrovirus, rotavirus, and male-specific coliphage systematically in fresh fruit and vegetables and associated agricultural environmental samples, including irrigation water, soil, and worker's gloves. Enteric viruses were detected by international standard methods (ISO/TS 15216), and male-specific coliphages were isolated using US EPA Method 1601. For the study, 773 samples were collected from June 2016 to April 2017, including Chinese cabbage (n=244), cucumber (n=98), lettuce (n=73), strawberry (n=120), soil (n=191), irrigation water (n=14), and gloves (n=27). Two cucumber and two irrigation water samples were positive for norovirus GI, and one cucumber and two irrigation water samples were positive for norovirus GII. HAV was detected in one strawberry sample and one glove sample. The other tested foodborne viruses were not detected in any of the samples. Sixteen male-specific coliphages were isolated from Chinese cabbage, cucumber, lettuce, cherry tomato, soil, and irrigation water. The isolation of male-specific coliphage would be more practical to investigate the fecal contamination in produce rather than pathogenic viruses. </P>

      • 뇌전도 신호를 이용한 레이싱 시뮬레이터 차량의 공유형 제어 방법

        민창선(Changsun Min),한신(Shin Han) 한국자동차공학회 2013 한국자동차공학회 부문종합 학술대회 Vol.2013 No.5

        Brain-Computer Interface(BCI) System has been rapidly developed recently in the field of brain engineering. BCI is the system that can read and output user’s thought by analyzing brain waves and manipulate the connected machine. In this paper, I have researched driving simulation using EEG signal by implementing real time BCI system of TORCS and tried to introduce a new interface as well as optimize it. First, I have determined electrodes that will be used as a control signal by analyzing the brain activation data gained through experimentation according to motor image. Then, I created a client to use as a control interface, and connected TORCS server and BCI2000 each using UDP communication to construct a real-time BCI system. BCI2000 receives the EEG data coming from the EEG measuring equipment and does overall processing of the EEG, then finally generates the control signals. After that, client send the command such as steering, acceleration of the vehicle to TORCS server based on the control signals, and car moves. Then artificial intelligence has been implanted in the client to form the basic model of shared-control system. And I inserted algorithm that can adjust and mediate the commands coming from each of the EEG and artificial intelligence to create improved shared-control model. As a test result, improved model of shared control was faster than the base model and artificial intelligence in lap times and top speed except for track with severe curves. Also the test participants showed more smooth and easy driving in case of improved model.

      • Novel TaqMan Real-Time PCR Method for Quantification of Potentially Hazardous Vibrio mimicus in Aquatic Products

        Hui Ryoon Shin,Sun Min Park,Changsun Choi,Min Suk Rhee 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

        Vibrio mimicus, a widely distributed bacteria in the aquatic environment, is a potential pathogen capable of causing foodborne outbreaks. Although conventional PCR methods for qualitative analysis of V. mimicus in foods have been developed, no quantitative analysis method has yet been established. In this study, novel TaqMan real-time PCR primers/probes targeting the sodB gene were designed and verified in a food system. The specificity of the designed primerprobe set was verified by the alignment of sequences in the NCBI database. Optimal annealing temperature to obtain the lowest Cq without nonspecific amplification was established by performing melting curve analysis of SYBR green real-time PCR. Crossreactivity was not shown with food matrices or any other food pathogens (V. parahaemolyticus, V. cholerae, V. fluvialis, Escherichia coli O157H7, Salmonella Typhimurium, and Staphylococcus aureus). Validation with artificially inoculated foods showed high sensitivity (detection limit < 1 log CFU/g). A newly developed method in this study is appliable to the surveillance of V. mimicus in foods, which could play an important role in advancing epidemiological research on V. mimicus.

      • Establishment of Pre-Treatment Method of Meat for Detection of Potentially Hazardous Streptococcus dysgalactiae

        Hui Ryoon Shin,Sun Min Park,Changsun Choi,Min Suk Rhee 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

        Streptococcus dysgalactiae, which causes invasive human infections, is zoonotic, and at the same time, a possible causative agent of food poisoning. Yet, there is no standardized detection method for S. dysgalactiae in food matrices. In this study, we developed both qualitative and quantitative analysis methods for S. dysgalactiae in meats including pre-treatment methods to inhibit meat debris from interrupting accurate detection. Low-speed centrifugation, lipase, or proteinase K treatment was introduced to enhance the DNA yield from the contaminated meat, which was confirmed with conventional, and real-time PCR. Optimal DNA extraction protocol including low-speed centrifugation at 600×g for 10 min, treatment of lipase (5 U/μ L), and proteinase K (2.76 mg/μL) was established. Validation was conducted with artificially inoculated meat in both high (ca. 3 log CFU/g) or low (ca. 1 log CFU/g) bacteria populations, and none of the false-positive or-negative results were shown. This study highlights the development of a simple and low-cost S. dysgalactiae detection method for meat which could contribute to the prevention of potential foodborne diseases in advance.

      • KCI등재

        u-병원 정보 시스템의 응용 서비스를 위한 멀티에이전트 기반 분산 프레임워크 구축

        정창원(ChangWon Jeong),신창선(ChangSun Shin),주수종(SuChong Joo) 한국정보과학회 2009 정보과학회 컴퓨팅의 실제 논문지 Vol.15 No.11

        최근 병원 환경은 점차 유비쿼터스 환경으로 변화되고 있으며, 이에 따라 응용 서비스 또한 새로운 요구사항에 직면하고 있다. 특히, 다양한 모바일 장치의 출현과 무선 센서 네트워크 기술의 도입은 u-헬스케어의 실현을 가속화 시키고 있다. 서로 다른 환경에서 구축된 정보의 통합이나 다양한 응용 시나리오를 만족하기 위해 멀티에이전트 패러다임을 도입하고 있는 추세이다. 본 논문은 유비쿼터스 병원 정보 시스템을 위한 소프트웨어 구조와 u-응용서비스에 대해 기술한다. 또한 본 연구에서는 u-병원 정보 시스템을 위해 멀티에이전트 기반 분산 프레임워크를 구축하고 제시하고 있다. 이는 JADE와 분산객체그룹 프레임워크를 포함한다. 그리고 의사와 간호사를 위해 환자의 건강 정보와 병실 환경 정보를 제공하는 u-응용 서비스를 구현하였다. 특히 기존 관련 연구에서 강조하고 있지 않은 보안 부분에 대한 상황기반의 동적 보안 메커니즘을 적용하였으며, 본 연구에서는 각 사용자의 GUI를 통해 수행결과를 보였다. As the hospital environment has been increasingly changed into a ubiquitous environment, the application services for the hospital environment are also faced with new requirements. In particular, the emergence of various mobile devices and the introduction of a wireless sensor network technology have accelerated the realization of the u-healthcare. The multi-agent paradigm has been introduced for satisfying both the integration of information and the various application scenarios established from various environments. This paper describes the software structure and uapplications for u-hospital information system based on ubiquitous environments. And it suggested the construction of a multi agent based distributed framework for supporting u-hospital information system. The suggested framework includes the JADE and distributed object group framework. And it implemented u-application services for supporting doctors and nurses, which provides the patient's health information and ward environment information. Especially, it is using a dynamic security mechanism on the security situation, which has not been emphasized in existing researches, and this paper shows the results for each user through the GUI.

      • KCI등재

        Moderate-Intensity Exercise Preserves Bone Mineral Density and Improves Femoral Trabecular Bone Microarchitecture in Middle-Aged Mice

        Seungyong Lee,Yun-A Shin,Jinkyung Cho,Dong-Ho Park,Changsun Kim 대한골대사학회 2022 대한골대사학회지 Vol.29 No.2

        Background: Aging leads to significant bone loss and elevated osteoporosis risk. Exercise slows age-related bone loss; however, the effects of various moderate-intensity exercise training volumes on bone metabolism remain unclear. This study aimed to determine the degree to which different volumes of moderate-intensity aerobic exercise training influence bone mineral density (BMD), bone mineral content (BMC), femoral trabecular bone microarchitecture, and cortical bone in middle-aged mice. Methods: Twenty middle-aged male C57BL/6 mice were randomly assigned 8 weeks of either (1) non-exercise (CON); (2) moderate-intensity with high-volume exercise (EX_MHV); or (3) moderate-intensity with low-volume exercise (EX_MLV) (N=6-7, respectively). Femoral BMD and BMC were evaluated using dual energy X-ray absorptiometry, and trabecular and cortical bone were measured using micro-computed tomography. Results: Femoral BMD in EX_MHV but not EX_MLV was significantly higher (P<0.05) than in CON. The distal femoral fractional trabecular bone volume/tissue volume (BV/TV, %) was significantly higher (P<0.05) in both EX_MHV and EX_MLV than in CON mice. Increased BV/TV was induced by significantly increased trabecular thickness (mm) and tended to be higher (P<0.10) in BV (mm3) and lower in trabecular separation (mm) in EX_MHV and EX_MLV than in CON. The femoral mid-diaphysis cortical bone was stronger in EX_MLV than EX_MHV. Conclusions: Long-term moderate-intensity aerobic exercise with low to high volumes can be thought to have a positive effect on hindlimb BMD and attenuate age-associated trabecular bone loss in the femur. Moderate-intensity aerobic exercise may be an effective and applicable exercise regimen to prevent age-related loss of BMD and BV.

      • KCI등재

        중년 남성의 흡연습관과 신체활동이 백혈구 텔로미어 길이에 미치는 영향

        신윤아(Yun-A Shin),박동호(Dong-Ho Park),김창선(Changsun Kim) 한국생활환경학회 2020 한국생활환경학회지 Vol.27 No.4

        This study was to investigate the effects of smoking habits and physical activity on telomere length in middle aged men. This study was conducted on 106 middle aged men. We examined smoking habites such as smoking period and amount per week, physical activity inflammation markers including c-reactive protein (CRP) and lactate dehycrogenase (LDH), oxidative stress marker (8-OHG), and telomere length. Waist circumference were significant difference between smoking and non-smoking groups (p<.05). Physical activity (PA) were not difference between smoking and non-smoking groups. LDH levels significant difference between smoking and non-smoking groups (p<.05). High-intensity PA has shown a negative association with LDH levels (r=-.117, p<.05) and 8-OHdG (r=-.234, p<.05). Moderate-intensity PA frequency has shown a negative association with 8-OHdG (r=-.223, p<.05). Telomere length has shown a negative association with smoking period (r=-.340, p<.05) and LDH levels (r=-.239, p<.01). These results suggested that smoking and PA affected inflammation and oxidative stress, but did not affect telomere because it was not clinically meaningful. Therefore, it is considered that further research is needed to analyze the chnages according to observation at various time points in the long period.

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