Antiproliferative Activities of Parthenolide and Golden Feverfew Extract Against Three Human Cancer Cell Lines

Changqing Wu,Feng Chen,James W. Rushing,Xi Wang,김현진,George Huang,Vivian Haley-Zitlin,Guoqing He 한국식품영양과학회 2006 Journal of medicinal food Vol.9 No.1

The medicinal herb feverfew [Tanacetum parthenium (L.) Schultz-Bip.] has long been used as a folk remedyfor the treatment of migraine and arthritis. Parthenolide, a sesquiterpene lactone, is considered to be the primary bioactivecompound in feverfew having anti-migraine, anti-tumor, and anti-inflammatory properties. In this study we determined, throughin vitrobioassays, the inhibitory activity of parthenolide and golden feverfew extract against two human breast cancer celllines (Hs605T and MCF-7) and one human cervical cancer cell line (SiHa). Feverfew ethanolic extract inhibited the growthof all three types of cancer cells with a half-effective concentration (EC50) of 1.5 mg/mL against Hs605T, 2.1 mg/mL againstMCF-7, and 0.6 mg/mL against SiHa. Among the tested constituents of feverfew (i.e., parthenolide, camphor, luteolin, andapigenin), parthenolide showed the highest inhibitory effect with an EC50 against Hs605T, MCF-7, and SiHa of 2.6 .g/mL,2.8 .g/mL, and 2.7 .g/mL, respectively. Interactions between parthenolide and flavonoids (apigenin and luteolin) in fever-few extract also were investigated to elucidate possible synergistic or antagonistic effects. The results revealed that apigeninand luteolin might have moderate to weak synergistic effects with parthenolide on the inhibition of cancer cell growth ofHs605T, MCF-7, and SiHa.

Prediction of exploration targets based on integrated analyses of source rock and simulated hydrocarbon migration direction: a case study from the gentle slope of Shulu Sag, Bohai Bay Basin, northern China

Changqing Ren,Fugui He,Xianzhi Gao,Dongsheng Wu,Wenli Yao,Jianzhang Tian,Huiping Guo,Yuanxin Huang,Li Wang,Han Feng,Junwei Li 한국지질과학협의회 2019 Geosciences Journal Vol.23 No.6

The Shulu Sag which is a rifted sag with NNE trend is located in the south of Jizhong Depression, Bohai Bay Basin, northern China. The gentle slope and three troughs are situated in the west and east of the sag, respectively. Both of the lower part of Shasan Member (Es3x) and the lower part of Shayi Member (Es1x) act as source rocks in this sag. Researches on the type, quantity, quality and thermal maturity of the respective organic matter have been conducted using Rock-Eval pyrolysis data. Type II is the dominant kerogen in Es1x of all troughs. However, Type II1 and III is the dominant kerogen in Es3x of Middle-Southern and Northern trough, respectively. TOC (total organic carbon) and pyrolysis S2 (hydrocarbon) values suggest that the Es1x source rocks in Middle-Southern and Northern trough are fair to good and poor to fair generative potential of hydrocarbon, separately. The Es3x source rocks in Middle-Southern and Northern trough possess fair to excellent and poor to fair generative potential of hydrocarbon, individually. Tmax (pyrolysis temperature at maximum S2) values indicate that most of Es3x samples are thermally mature, but all Es1x samples are thermally immature. Under large scale condition, the hydrocarbon secondary migration in the upper part of Shasan Member (Es3s), Shaer Member (Es2) and the upper part of Shayi Member (Es1s) have been simulated using fluid potential model with Arcgis 9.3 software. The simulation results reveal the direction of hydrocarbon secondary migration and the distribution of hydrocarbon migration-accumulation units (HMAUS), and also suggest that the hydrocarbon migration direction is obviously controlled by nose-like structure belts where most of hydrocarbons accumulate. That shows high reliability because they are consistent with the hydrocarbon exploration result in this area. On the basis of integrated analyses of source rocks and hydrocarbon migration direction, the following five areas in the gentle slope are identified to be the preferred hydrocarbon accumulation area: Taijiazhuang area, northern and southern Xicaogu area, as well as northern and southern Leijiazhuang area. It is considerably helpful to reduce the risk in hydrocarbon exploration of Shulu Sag.

Comparison of overfed Xupu and Landes geese in performance, fatty acid composition, enzymes and gene expression related to lipid metabolism

Liu Xu,Li Peng,He Changqing,Qu Xiangyong,Guo Songchang 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.12

Objective: The aim of this study was to compare overfeeding performance, fatty acid composition, blood chemistry, enzymes and genes expression overfed Xupu and Landes geese. Methods: Sixty male Xupu geese (80 d) and Landes geese (80 d) were selected. After a period of one-week of pre-overfeeding, Xupu and Landes geese were overfed three meals of 550 and 350 g/d, respectively, of a high-carbohydrate diet in the first week of the overfeeding period. The next week, geese were given four meals of 1,200 and 850 g/d, respectively, over 8 to 14 d. Finally, geese were given five meals of 1,600 and 1,350 g/d, respectively, for the last two weeks. Results: After overfeeding for 28 d: Compared with Landes geese, Xupu geese liver weight and liver-to-body weight ratio decreased (p<0.05), while final weight, slaughter weight, total weight gain, abdominal fat weight, and feed-to-liver weight ratio increased (p<0.05). The levels of elaidic acid (C18:1t9), oleic acid (C18:1n-9), eicosenoic acid, and arachidonic acid in the liver of Xupu geese significantly increased (p<0.05), and the levels of myristic acid and stearic acid significantly decreased (p<0.05), while methyleicosanoate acid significantly increased (p<0.05). Xupu geese had higher plasma concentrations of triglyceride and very low density lipoprotein cholesterol (p<0.05), and decreased activities of alanine aminotransferase, aspartate aminotransferase, and lipase (LPS) (p<0.05). Landes geese had higher LPS activity (p<0.05), but lower cholinesterase activity (p<0.05) when compared with Xupu geese. The mRNA expression levels of fatty acid dehydrogenase (FADS) gene, elongase of long-chain fatty acid 1 (ELOVL1) gene, ELOVL5, and acyl-Co A: cholesterol acyltransferase 2 (ACAT2) gene were significantly upregulated (p<0.05) in Landes goose when compared with Xupu geese. Conclusion: This study demonstrates that the liver production performance of Landes geese was better than that of Xupu geese to some extent, which may be closely related to LPS activity, as well as the expression of FADS, ELOVL1, ELOVL5, and ACAT2.

Strand-specific RNA sequencing in pig testes identifies developmentally regulated genes and circular RNAs

Maoliang Ran,Bo Weng,Maisheng Wu,Changqing He,Shanwen Zhang,Bin Chen 한국유전학회 2017 Genes & Genomics Vol.39 No.10

Testicular development and spermatogenesis are strictly regulated with the complex interactions between several cell types in testis. However, the limited availability of genomic and molecular information limits our understanding of this complex physiological process. In this study, we characterized the transcriptome between immature (30-day-old) and mature (180-day-old) pig testes using RNA-seq technology. 24,469 known coding gene transcripts corresponding to 20,566 genes were mapped in these two developmental stages, 3,328 genes were differentially expressed, and numerous novel transcripts and alternative splicing events were also identified. Ten differentially expressed genes were validated by measuring the relative expression using real-time quantitative polymerase chain reaction (PCR). 125 Gene Ontology (GO) terms were significantly enriched, and most of them involved in GO terms related to male reproduction, testicular development, and spermatogenesis. In addition, this study also represented the prediction of 10,000 circRNAs, as well as the validation of six pig circRNAs using Find_cric algorithm. Our study substantially expanded our knowledge about the transcriptomic profile of immature and mature pig testes, and provided a useful resource to study the mechanisms of pig testis development and spermatogenesis at the molecular level.

Coupled irradiation-thermal-mechanical analysis of the solid-state core in a heat pipe cooled reactor

Yugao Ma,Jiusong Liu,Hongxing Yu,Changqing Tian,Shanfang Huang,Jian Deng,Xiaoming Chai,Yu Liu,Xiaoqiang He 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.6

The solid-state core of a heat pipe cooled reactor operates at high temperatures over 1000 K withthermal and irradiation-induced expansion during burnup. The expansion changes the gap thicknessbetween the solid components and the material properties, and may even cause the gap closure, whichthen significantly influences the thermal and mechanical characteristics of the reactor core. This studydeveloped an irradiation behavior model for HPRTRAN, a heat pipe reactor system analysis code, tointroduce the irradiation effects such as swelling and creep. The megawatt heat pipe reactor MegaPowerwas chosen as an application case. The coupled irradiation-thermal-mechanical model was developed tosimulate the irradiation effects on the heat transfer and stresses of the whole reactor core. The resultsshow that the irradiation deformation effect is significant, with the irradiation-induced strains up to2.82% for fuel and 0.30% for monolith at the end of the reactor lifetime. The peak temperatures during thelifetime are 1027:3 K for the fuel and 956:2 K for monolith. The gap closure enhances the heat transferbut caused high stresses exceeding the yield strength in the monolith

Systematic identification and characterization of miRNAs and piRNAs from porcine testes

Bo Weng,Maoliang Ran,Maisheng Wu,Fuzhi Peng,Lianhua Dong,Changqing He,Shanwen Zhang,Zhaohui Li,Bin Chen 한국유전학회 2017 Genes & Genomics Vol.39 No.10

microRNAs (miRNAs) and PIWI-interacting RNAs (piRNAs) execute important regulatory roles in testis development and spermatogenesis, while previous studies mainly focus on the expression profiles in immature and mature porcine testes, which may cause a bottleneck for further understanding their complex physiological processes in porcine testes development and spermatogenesis. Thus, we presented the expression and characterization of miRNAs and piRNAs in DS (60-day-old), DN (90-dayold), DT (120-day-old) and DF (150-day-old) pig testes. In total, 12,834,628, 13,359,726, 12,851,249 and 12,938,601 clean reads were generated from these libraries, respectively. 293 mature and 36 novel miRNAs as well as 4923 piRNA clusters were identified from pig testes, and they showed an age-dependent manner. GO enrichment analysis of miRNA target genes and piRNA generated genes showed that they participated widely in regulating the pig spermatogenesis process. In addition, 12 differentially expressed miRNAs were randomly selected to validate using qRT-PCR. Our results provided novel comprehensive expression profiles of miRNAs and piRNAs in pig testes at different stages of sexual maturity, which will promote our knowledge of them in regulating the pig testes development and spermatogenesis process.