http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Effects of Polycyclic Aromatic Hydrocarbons on DNA Damage and Plasma Protein Expression in Mouse
Oh, Sang-Nam,Oh, Eun-Ha,Im, Ho-Sub,Jo, Gyu-Chan,Sul, Dong-Geun,Kim, Young-Whan,Lee, Eun-Il The Korean Society of Toxicogenomics and Toxicopro 2005 Molecular & cellular toxicology Vol.1 No.1
Polycyclic aromatic hydrocarbons (PAHs) are an important class of environmentally prevalent xenobiotics that exert complex effects on the biological system and characterized as probably carcinogenic materials. Single cell gel electrophoresis assays were performed in order to evaluate DNA damage occurring in the T-and B lymphocytes, spleens (T/B-cell), bone marrow, and livers of mouse exposed to mixture of PAHs (Benzo(a)pyrene, Benzo(e)pyrene, Fluoranthene, Pyrene) at dose of 400, 800, or 1600 mg/kg body weight for 2 days. DNA damage of the cells purified from mice was increased in dose dependent manner. In the blood cells and organs, DNA damage was also discovered to vary directly with PAHs. Especially T-cells had been damaged more than B-cell. Plasma proteomes were separated by 2-dimensional electrophoresis with pH 4-7 ranges of IPG Dry strips and many proteins showed significant up-and -down expressions with the dose dependent manner. Of these, significant 4 spots were identified using matrix-assisted laser desorption/ionization-time of fight (MALDI-TOF) mass spectrometry. Identified proteins were related to energy metabolism and signal transduction.
Impedance Variation on Lattice Misoriented Few-Layer Graphene Via Layer Decoupling
Kim, Whan Kyun,Oh, Juyeong,Yoon, Hyong Seo,Kim, Sun Jun,Park, Jae Young,Jung, Jeil,Chan Jun, Seong IEEE 2019 IEEE TRANSACTIONS ON NANOTECHNOLOGY Vol.18 No.-
<P>We demonstrate radio frequency (RF) transmission characteristics in a layer stacked manner. Generally, as the number of graphene layers increases, the impedance is reduced and the transmission efficiency of monolayer graphene becomes better than that of chemically synthesized monolayer graphene. Depending on the stacking method, graphene may or may not be well aligned between layers; however, this characteristic affects the RF transmission. Graphene extracted from graphite is well aligned with other layers; however, synthesized graphene is randomly stacked because it is transferred and the layers are not aligned. Scattering parameters and the measured impedance shows that graphene extracted from graphite exhibits better transmission characteristics in the case of single-layer graphene, whereas synthesized graphene shows better transmission characteristics in the case of multiple-layer graphene. We calculated the change in conductivity based on the matched angle between layers of graphene and found that the conductivity was higher when the matching was less, compared to when it was well-matched. These results are also reflected in the impedance extracted from the scattering parameters. It was found that different impedance patterns are formed in single-layer graphene and in multiple layers for multi-layer graphene with stacking methods. This is because the layers are decoupled when they are randomly stacked.</P>
스마트 도로 구현을 위한 e-Mobility 수용 및 C-ITS 활용방안 연구
권오용(KWON, Oh Yong),김영찬(KIM, Young Chan),배명환(BAE, Myoung Whan) 대한교통학회 2019 대한교통학회 학술대회지 Vol.80 No.-
현재 지능형교통체계 분야는 4차 산업혁명을 기조로 하는 정부정책과 개인 휴대단말과 선호 애플리케이션에 따른 이용자 패러다임 변화, 민간교통정보 활용 등 다양한 산업, 문화적 여건 변화로 단순한 교통정보 제공에서 교통사고 예방과 자율주행차 산업 지원이라는 자율협력주행체계로의 대변혁을 겪고 있다. 4차 산업혁명은 기존 1~3차 산업혁명 즉, 기계화, 제조업, 정보화에 따른 생산성 향상이 융복합되는 형태를 말하며, 요약하면 제조업과 ICT가 접목된 것이라는 단순한 예측으로부터, 모든 사물이 연결되어 현실과 가상의 구분이 없어질 것이라는 예측에 이르기까지 그 변화의 범위는 쉽게 단언할 수 없다. 본 논문에서는 정부에서 제시하는 4차산업혁명 융합서비스, 산업기반 및 지능형인프라 기술과 관련하여, 스마트도로 구현을 위한 지능형교통체계 분야가 갖춰야할 준비성을 객체지향적인 관점에서 검토하여 제시한다.
Kim, Jeong- Oh,Shin, Jung -Young,Kim, Min Young,Son, Kyoung Hwa,Jung, Chan Kwon,Kim, Tae-Jung,Kim, Su Young,Park, Jae Kil,Sung, Sook Whan,Bae, Sang Ju,Min, Hyun Jung,Kang, Jin- Hyoung Elsevier 2018 Surgical oncology Vol.27 No.1
<P><B>Abstract</B></P> <P><B>Background</B></P> <P>We screened resected tumor tissues from patients with lung cancer for <I>EGFR</I> mutations, <I>ALK</I> rearrangements, and rearranged during transfection (<I>RET</I>) gene variants (including <I>RET</I> rearrangements and the Kinesin Family Member 5B <I>(KIF5B</I>)<I>-RET</I> fusion gene) using various methods including reverse transcription polymerase chain reaction (RT-PCR), transcript assays, fluorescence <I>in situ</I> hybridization (FISH), and immunohistochemistry (IHC). We also examined the protein expression of associated downstream signaling molecules to assess the effect of these variants on patient outcome.</P> <P><B>Method</B></P> <P>We constructed a tissue microarray (TMA) comprising 581 resected tumor tissues from patients with lung adenocarcinoma and analyzed the microarray by both FISH (using <I>RET</I> break-apart and <I>KIF5B-RET</I> SY translocation probes) and a commercial <I>RET</I> transcript assay. We evaluated the expression of RET and RET-related signaling molecules, including p-AKT and p-ERK, by TMA -based IHC staining.</P> <P><B>Results</B></P> <P>Among the 581 specimens, 51 (8.8%) specimens harbored <I>RET</I> rearrangements, including 12 cases (2.1%) carrying a <I>KIF5B-RET</I> fusion gene. Surprisingly, RET expression was lower in <I>KIF5B-RET</I> fusion gene-positive than in <I>RET</I> wild-type specimens. We detected activating <I>EGFR</I> mutations in 11 (21.6%) of the 51 <I>RET</I> variant-positive specimens. Among the <I>KIF5B-RET</I> fusion gene-positive specimens, p-ERK expression was significantly lower in the <I>EGFR</I> mutation subgroup showing RET expression than in the <I>EGFR</I> mutation subgroup that did not express RET. Similarly, the <I>RET</I> rearrangement group showed significant variation in the expression level of p-AKT (P = 0.028) and p-ERK, whose expression remarkably increased in specimens not expressing RET. The expression of p-ERK markedly increased in the <I>RET</I> rearrangement group regardless of RET expression.</P> <P><B>Conclusion</B></P> <P>This result suggests that a combination of RET and ERK inhibitors may be an effective treatment strategy for lung adenocarcinoma patients harboring <I>RET</I> variants.</P> <P><B>Highlights</B></P> <P> <UL> <LI> FISH is clinically applicable as well as RT-PCR for <I>RET</I> variants analysis. </LI> <LI> We detected the <I>EGFR</I> mutation and <I>KIF5B-RET</I> fusion gene in lung cancer. </LI> <LI> Expression of <I>RET-</I>associated signaling molecules varied in <I>RET</I> variant-positive groups. </LI> <LI> The <I>KIF5B-RET</I> fusion gene increased and inhibited p-ERK expression. </LI> <LI> RET and ERK inhibitors may be combined to treat lung adenocarcinoma with <I>RET</I> variants. </LI> </UL> </P>