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Shope Rabbit Papillomavirus DNA의 Cloning 및 특성 규명
신승식,양용태,정상인,최철순,구자현,고재경 중앙대학교 의과대학 의과학연구소 1989 中央醫大誌 Vol.14 No.2
Shope rabbit papillomavirus (SRPV), a member of Genus papillomavirus of Family Papovariridae, causes benign tumors (papillomas) of squamous epithelium both in its natural host, i.e.,, cottontail rabbits and in its artificial host, i.e., domestic rabbits. So far, SRPV has never been successfully cultivated in tissue culture cells, and the lack of reliable tissue culture system for transformation assay has seriously hindered the progress in the oncogenic study of this well-known DNA tumor virus. Therefore, the only possible way of obtaining SRPV in, the laboratory is to inoculate cottontail rabbits with SRPV and to harvest the virus-loaded papilloma tissues. This study was undertaken 1) to isolate SRPV DNA from SRPV-induced papillomas of either cottontail rabbits or domestic rabbits, 2) to determine the restriction endonuclease cleavage sites of such purified SRPV DNA, 3) to carry out cloning of SRPV DNA in E. coli with the use of pBR322 vector and 4) to test the oncogenicity of cloned SRPV DNA-pBR322 recombinants in the domestic rabbits through inoculation experiments. The results are summarized as follows. 1. Though repeated attempts failed to isolate purified Shope rabbit papillomavirus DNA from the domestic rabbit papillomas (DRP), a good yields of purified SRPV DNA were successfully isolated from the cottontial rabbit papillomas (GRP). 2. With the purified SRPV DNA from GRP, the presence of cleavage sites for a number of restriction endonucleases was confirmed and extended, and the approximate molecular length of isolated SRPV DNA was determined to be 8 kbp. 3. Molecular cloning of isolated SRPV DNA was completed in E. coli YMC-10, using pBR322 as the vector, at the Eco RI and Sal I cleavage site, respectively. The cloned products were either Eco RI-site cloned SRPV DNA-pBR322 recombinant or Sal I site-cloned SRPV DAN-pBR322 recombinant. 4. Inoculations of cloned SRPV DNA-pBR322 recombinants into the domestic rabbits' skin failed to establish the oncogenic potential of the cloned products.