Phosphoproteomic profiling of myofibrillar and sarcoplasmic proteins of muscle in response to salting

Caixia Zhang,Zhenyu Wang,Zheng Li,Qingwu Shen,Lijuan Chen,Lingling Gao,Dequan Zhang 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4

A phosphoproteomic profile of myofibrillar and sarcoplasmic proteins of muscle inresponse to salting was investigated. Myofibrillar and sarcoplasmic proteins extracted from saltedmeat with 0, 1, 2, 3, 4, and 5% salt for 0, 2, 4, 6, 8, and 16 h were analyzed by SDS-PAGE electrophoresisand fluorescence staining. The global phosphorylation of myofibrillar proteins in salted meat was lowerthan that in control muscle at 16 h of salting (p<0.05), and the global phosphorylation of myofibrillarproteins in 3% salt-treated group at 16 h was the lowest. However, salting showed no significant effecton phosphorylation of sarcoplasmic proteins. Four categories of phosphorylated protein wereidentified by LC-MS/MS, involved in stress response (heat shock protein), glycometabolism (glycogenphosphorylase, glyceraldehyde-3-phosphate dehydrogenase), oxidation or reduction (superoxidedismutase), and others (myoglobin), the phosphorylation of which was affected by salting. Thus,salting may influence meat quality through protein phosphorylation, which regulates proteindegradation and glycolysis.

Phosphoproteomic profiling of myofibrillar and sarcoplasmic proteins of muscle in response to salting

Zhang, Caixia,Wang, Zhenyu,Li, Zheng,Shen, Qingwu,Chen, Lijuan,Gao, Lingling,Zhang, Dequan 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4

A phosphoproteomic profile of myofibrillar and sarcoplasmic proteins of muscle in response to salting was investigated. Myofibrillar and sarcoplasmic proteins extracted from salted meat with 0, 1, 2, 3, 4, and 5% salt for 0, 2, 4, 6, 8, and 16 h were analyzed by SDS-PAGE electrophoresis and fluorescence staining. The global phosphorylation of myofibrillar proteins in salted meat was lower than that in control muscle at 16 h of salting (p<0.05), and the global phosphorylation of myofibrillar proteins in 3% salt-treated group at 16 h was the lowest. However, salting showed no significant effect on phosphorylation of sarcoplasmic proteins. Four categories of phosphorylated protein were identified by LC-MS/MS, involved in stress response (heat shock protein), glycometabolism (glycogen phosphorylase, glyceraldehyde-3-phosphate dehydrogenase), oxidation or reduction (superoxide dismutase), and others (myoglobin), the phosphorylation of which was affected by salting. Thus, salting may influence meat quality through protein phosphorylation, which regulates protein degradation and glycolysis.

FA/Mel@ZnO nanoparticles as drug self-delivery systems for RPE protection against oxidative stress

Yi, Caixia,Yu, Zhihai,Sun, Xin,Zheng, Xi,Yang, Shuangya,Liu, Hengchuan,Song, Yi,Huang, Xiao Techno-Press 2022 Advances in nano research Vol.13 No.1

Drug self-delivery systems can easily realize combination drug therapy and avoid carrier-induced toxicity and immunogenicity because they do not need non-therapeutic carrier materials. So, designing appropriate drug self-delivery systems for specific diseases can settle most of the problems existing in traditional drug delivery systems. Retinal pigment epithelium is very important for the homeostasis of retina. However, it is vulnerable to oxidative damage and difficult to repair. Worse still, the antioxidants can hardly reach the retina by non-invasive administration routes due to the ocular barriers. Herein, the targeted group (folic acid) and antioxidant (melatonin) have been grafted on the surface of ZnO quantum dots to fabricate a new kind of drug self-delivery systems as a protectant via eyedrops. In this study, the negative nanoparticles with size ranging in 4~6 nm were successfully synthesized. They could easily and precisely deliver drugs to retinal pigment epithelium via eyedrops. And they realized acid degradation to controlled release of melatonin and zinc in retinal pigment epithelium cells. Consequently, the structure of retinal pigment epithelium cells were stabilized according to the expression of ZO-1 and β-catenin. Moreover, the antioxidant capacity of retinal pigment epithelium were enhanced both in health mice and photic injury mice. Therefore, such new drug self-delivery systems have great potential both in prevention and treatment of oxidative damage induced retinal diseases.

Estrogen promotes the onset and development of idiopathic scoliosis via disproportionate endochondral ossification of the anterior and posterior column in a bipedal rat model

Shuhui Zheng,Hang Zhou,Bo Gao,Yongyong Li,Zhiheng Liao,Taifeng Zhou,Chengjie Lian,Zizhao Wu,Deying Su,Tingting Wang,Peiqiang Su,Caixia Xu 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

This study aimed to verify the effects of estrogen on the onset and development of adolescent idiopathic scoliosis and the mechanisms associated with these effects by constructing a pubescent bipedal rat model. Experiments were conducted to investigate whether scoliosis progression was prevented by a Triptorelin treatment. One hundred twenty bipedal rats were divided into female, OVX (ovariectomy), OVX + E2, Triptorelin, sham, and male groups. According to a spinal radiographic analysis, the scoliosis rates and curve severity of the female and OVX + E2 groups were higher than those in the OVX, Triptorelin, and male groups. The measurements obtained from the sagittal plane of thoracic vertebrae CT confirmed a relatively slower growth of the anterior elements and a faster growth of the posterior elements between T11 and T13 in the female and OVX + E2 groups than in the OVX and Triptorelin groups. Histomorphometry and immunohistochemistry revealed a significantly longer hypertrophic zone of the vertebral cartilage growth plates that expressed more type X collagen and less type II collagen in the OVX and Triptorelin groups than in the female and OVX + E2 groups. Ki67 immunostaining confirmed an increase in the proliferation of vertebral growth plate chondrocytes in the OVX group compared with the female and OVX + E2 groups. In conclusion, estrogen obviously increased the incidence of scoliosis and curve severity in pubescent bipedal rats. The underlying mechanism may be a loss of coupling of the endochondral ossification between the anterior and posterior columns. Triptorelin decreased the incidence of scoliosis and curve magnitudes in bipedal female rats. Introduction Adolescent

The domestication event of the Tibetan pig revealed to be in the upstream region of the Yellow River based on the mtDNA D-loop

Ge, Qianyun,Gao, Caixia,Cai, Yuan,Jiao, Ting,Quan, Jinqiang,Guo, Yongbo,Zheng, Wangshan,Zhao, Shengguo Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.4

Objective: Evidence from previous reports indicates that pig domestication in East Asia mainly occurred in the Mekong region and the middle and downstream regions of the Yangtze River. Further research identified two new origin centers for domestic pigs in the Tibetan Plateau and the islands of Southeast Asia. However, due to the small sample size of Tibetan pigs, details of the origin and spread of Tibetan pigs has not yet been established. Methods: We analyzed mitochondrial DNA control region (D-loop) variation in 1,201 individuals from nine Tibetan pig populations across five provinces. Comprehensive Tibetan pig samples were taken to perform the most detailed analysis of Tibetan pigs to date. Results: The result indicate that Rkaze pigs had the lowest level of diversity, while Changdu pigs had the highest diversity. Interestingly, these two populations were both in the Tibetan Plateau area. If we calculate diversity in terms of each province, the Tibetan Plateau area had the lowest diversity, while the Chinese province of Gansu had the highest diversity. Diversity gradient analysis of major haplotypes suggested three domestication centers of Tibetan pigs in the Tibetan Plateau and the Chinese provinces of Gansu and Yunnan. Conclusion: We found two new domestication centers for Tibetan pigs. One is in the Chinese province of Gansu, which lies in the upstream region of the Yellow River, and the other is in the Chinese province of Yunnan.

Evaluating genetic diversity and identifying priority conservation for seven Tibetan pig populations in China based on the mtDNA D-loop

Ge Qianyun,Gao Caixia,Cai Yuan,Jiao Ting,Quan Jinqiang,Guo Yongbo,Zheng Wangshan,Zhao Shengguo 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.12

Objective: Tibetan pigs, an excellent species unique to China, face serious threats, which in turn affects the development and utilization of the outstanding advantages of plateau hypoxia adaptability and reduces their genetic diversity. Therefore, a discussion of measures to conserve this genetic resource is necessary. The method, based on genetic diversity, genetic divergence and total genetic contribution rate of population, reflects the priority conservation order and varies depending on the three different purposes of conservation. Methods: We analyzed mitochondrial DNA control region (D-loop) variation in 1,201 individuals from nine Tibetan pig populations across five provinces and downloaded 564 mtDNA D-loop sequences from three indigenous pig breeds in Qinghai, Sichuan, and Yunnan Provinces distributed near the Tibetan pigs. Results: We analyzed three different aspects: Changdu Tibetan pigs have the highest genetic diversity, and from the perspective of genetic diversity, the priority conservation is Changdu Tibetan pigs. Hezuo Tibetan pigs have the highest genetic contribution, so the priority conservation is Hezuo Tibetan pigs in the genetic contribution aspect. Rkaze Tibetan pigs were severely affected by indigenous pig breeds, so if considering from the perspective of introgression, the priority conservation is Rkaze Tibetan pigs. Conclusion: This study evaluated genetic diversity and comprehensively assessed conservation priority from three different aspects in nine Tibetan pig populations.

Role of Myocardial Extracellular Volume Fraction Measured with Magnetic Resonance Imaging in the Prediction of Left Ventricular Functional Outcome after Revascularization of Chronic Total Occlusion of Coronary Arteries

Yinyin Chen,Xinde Zheng,Hang Jin,Shengming Deng,Daoyuan Ren,Andreas Greiser,Caixia Fu,Hongxiang Gao,Mengsu Zeng 대한영상의학회 2019 Korean Journal of Radiology Vol.20 No.1

Objective: The purpose of this study was to prospectively investigate the value of the myocardial extracellular volume fraction (ECV) in predicting myocardial functional outcome after revascularization of coronary chronic total occlusion (CTO). Materials and Methods: Thirty patients with CTO underwent cardiovascular magnetic resonance (CMR) before and 6 months after revascularization. Three baseline markers of functional outcome were evaluated in the dysfunctional segments assigned to the CTO vessels: ECV, transmural extent of infarction (TEI), and unenhanced rim thickness (RIM). At the global level, the ECV values of the whole myocardium with and without a hyperenhanced region (global and remote ECV) were respectively measured. Results: In per-segment analysis, ECV was superior to TEI and RIM in predicting functional recovery (area under receiver operating characteristic curve [AUC]: 0.86 vs. 0.75 and 0.73, all p values < 0.010), and it emerged as the only independent predictor of regional functional outcome (odds ratio [OR] = 0.83, 95% confidence interval [CI]: 0.77–0.89; p < 0.001) independent of collateral circulation. In per-patient analysis, global baseline ECV was indicative of ejection fraction (EF) at the follow-up examination (β = -0.61, p < 0.001) and changes in EF (β = -0.57, p = 0.001) in multivariate regression analysis. A patient with global baseline ECV less than 30.0% (AUC, 0.93; sensitivity 94%, specificity 80%) was more likely to demonstrate significant EF improvement (OR: 0.38; 95% CI: 0.17–0.85; p = 0.019). Conclusion: Extracellular volume fraction obtained by CMR may provide incremental value for the prediction of functional recovery both at the segmental and global levels in CTO patients, and may facilitate the identification of patients who can benefit from revascularization.

P(BA-co-HBA) Coated Fe3O4@ZnO Nanoparticles as Photo-Responsive Multifunctional Drug Delivery Systems for Safer Cancer Therapy

Xiao Huang,Xi Zheng,Caixia Yi,Shuanghong Yin 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.5

Amphipathic polymer coated magnetic nanoparticles which are loaded with docetaxel as photoresponsive multifunctional drug delivery systems for safer cancer therapy have been fabricated. Such kind of drug delivery system not only possesses favorable magnetic response property, but also could realize docetaxel-release and EGF-adsorption simultaneously simulated by UV and dark control, which can significantly boost the effectiveness of chemotherapy.

Expression patterns of octoploid strawberry TGA genes reveal a potential role in response to Podosphaera aphanis infection

Jun Feng,Yang Cheng,Caixia Zheng 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1

TGA family transcription factors are downstream components of the salicylic acid (SA) pathway and play a crucial role in the penetration defense against powdery mildew, a disease caused by the biotrophic pathogen Podosphaera aphanis that can cause enormous financial losses. However, the TGA genes in the octoploid strawberry (Benihoppe) remain largely unexplored. In this study, 11 FaTGA genes were identified and grouped into five subgroups based on the Strawberry GARDEN database. The evolution of TGAs has been relatively conservative, maintaining nearly the same protein domain and motif architectures. RT-qPCR showed that FaTGA s have different expression patterns in roots, stems, and leaves. The characteristics of their gene expression patterns indicate that FaTGA1, FaTGA2, FaTGA5, FaTGA7, FaTGA8, FaTGA9, and FaTGA10, as well as SA-responsive genes, were responsive to P. aphanis infection. In particular, exogenous SA induced significant upregulation of FaTGA and SA-responsive genes. Further correlation analysis suggested that FaTGA1, FaTGA2, FaTGA5, FaTGA7, FaTGA8, and FaTGA10 may play a crucial role in the response to powdery mildew disease. Taken together, our results reveal the responses of TGA genes in the strawberry P. aphanis interaction, which will provide further insight into the strawberry defense against powdery mildew.

Scaling analysis of magnetic-thermal behaviors in ferromagnetic insulator LaCoO3 thin film

Liu Hao,Fan Jiyu,Zheng Huan,Rahman Azizur,Qian Fengjiao,Wang Caixia,Ma Chunlan,Zhu Yan,Yang Hao 한국물리학회 2021 Current Applied Physics Vol.28 No.-

The structure, surface topography and magnetic properties of LaCoO3 (LCO) thin films deposited epitaxially on the SrTiO3 substrates have been investigated in detail. The LCO thin films show a typical ferromagneticparamagnetic (FM-PM) phase transition at 78 K. Based on the measurements of isothermal magnetization around the Curie point, the magnetic entropy change (ΔSM) of LCO thin films under various applied magnetic fields were obtained. By utilizing scaling theory, all of the ΔSM curves can be re-scaled, confirming that the FMPM phase transition is second-order magnetic phase transition. Moreover, the magnetic entropy change -ΔSM features a maximum around TC, whereas, power law fitting of -ΔSmax M with H gives n = 0.9704, which is obviously deviating from the standard value n = 2/3 for the Landau mean field model. This indicates that the mean field theory cannot be used to explain the critical behavior of LCO thin films which implies that the critical behavior of LCO thin films may involve complex magnetic interactions. These interactions are correlated with a long-term puzzling finding in this system why its Curie point is always around 80 K regardless of the different fabrication methods and conditions used in the film growth.