Effects of Different Fatty Acids and Levels on the Lipogenesis Capacity and Lipolysis Rate of Broilers In Vitro

Lien, T.F.,Wu, C.P.,Chen, K.L.,Yang, K.H. Asian Australasian Association of Animal Productio 2000 Animal Bioscience Vol.13 No.9

This study investigated the lipogenesis capacity of hepatocytes and lipolysis rate of adipocytes of broilers as affected by different fatty acids (trial one) and different linoleic acid (C18:2) levels (trial two). Twenty 6-wk old broilers were used; their hepatocytes and adipocytes were isolated for the in vitro study. In trial one, four treatments were tested. The control group in which no fatty acid was added, and the test groups to which were added $300{\mu}M$ of C16:0, C18:1 and C18:2, respectively. For trial two, different levels (0, $300{\mu}M$ and 1 mM) of C18:2 combined to fatty acid-free bovine serum albumin (BSA) were added to the medium. According to results of trial one, added fatty acids significantly reduced the incorporation by hepatocytes of [U,$^{14}C$]glucose into total lipid (p<0.05); the lipogenesis capacity in C18:2 group was the lowest. Although a similar pattern was found with [l,$^{14}C$]acetate, the groups only slightly differed in terms of lipogenesis capacity (p=0.11). In addition, the C18:2 group had a significantly (p<0.05) greater lipolysis rate than the C16:0 and control groups. Results of trial two indicated that C18:2 significantly (p<0.05) reduced lipogenesis capacity both for [U,$^{14}C$]glucose and [l,$^{14}C$]acetate, and markedly stimulated the lipolysis rate (p<0.05), displaying a dose response. Results presented herein demonstrate that C18:2 can reduce lipogenesis capacity and stimulate the lipolysis rate in broilers.

C₂H <i>N</i> = 1 − 0 and N₂H⁺<i>J</i> = 1 − 0 observations of <i>Planck</i> Galactic cold clumps

Liu, X.-C.,Wu, Y.,Zhang, C.,Liu, T.,Yuan, J.,Qin, S.-L.,Ju, B.-G.,Li, L.-X. EDP Sciences 2019 Astronomy and astrophysics Vol.622 No.-

<P>A survey of C2H <I>N</I> = 1 − 0 and N2H<SUP>+</SUP><I>J</I> = 1 − 0 toward <I>Planck</I> Galactic cold clumps (PGCCs) was performed using the Purple Mountain Observatory’s 13.7 m telescope. C2H and N2H<SUP>+</SUP> were chosen to study the chemical evolutionary states of PGCCs. Among 121 observed molecular cores associated with PGCCs, 71 and 58 are detected with C2H <I>N</I> = 1 − 0 and N2H<SUP>+</SUP><I>J</I> = 1 − 0, respectively. The detected lines of most sources can be fitted with a single component with compatible <I>V</I>LSR and line widths, which confirms that these PGCC cores are very cold (with gas temperatures 9-21 K) and quiescent while still dominanted by turbulence. The ratio between the column densities of C2H and N2H<SUP>+</SUP> (<I>N</I>(C2H)/<I>N</I>(N2H<SUP>+</SUP>)) is found to be a good tracer for the evolutionary states of PGCC cores. Gas-grain chemical model can reproduce the decreasing trend of <I>N</I>(C2H)/<I>N</I>(N2H<SUP>+</SUP>) as a function of time. The cores with the lowest abundances of N2H<SUP>+</SUP> (<I>X</I>[N2H<SUP>+</SUP>] < 10<SUP>−10</SUP>) are the youngest, and have nearly constant abundances of C2H. In evolved cores with <I>X</I>[N2H<SUP>+</SUP>] ~10<SUP>−9</SUP>, abundances of C2H drop quickly as the exhaustion of carbon atoms. Although these PGCC cores are in different evolutionary states, they are all quite young (< 5 × 10<SUP>5</SUP> yr) with <I>N</I>(C2H) > <I>N</I>(N2H<SUP>+</SUP>). Mapping observations are carried out toward 20 PGCC cores. The PGCC cores in Cepheus have lower <I>N</I>(C2H)/<I>N</I>(N2H<SUP>+</SUP>) and larger line widths compared with those in Taurus. This implies that PGCC cores in Taurus are less chemically evolved than those in Cepheus.</P>

Distribution of stress state in the Nankai subduction zone, southwest Japan and a comparison with Japan Trench

Lin, W.,Byrne, T.B.,Kinoshita, M.,McNeill, L.C.,Chang, C.,Lewis, J.C.,Yamamoto, Y.,Saffer, D.M.,Casey Moore, J.,Wu, H.Y.,Tsuji, T.,Yamada, Y.,Conin, M.,Saito, S.,Ito, T.,Tobin, H.J.,Kimura, G.,Kanagaw Elsevier Scientific Publishing Co 2016 Tectonophysics Vol.692 No.2

<P>To better understand the distribution of three dimensional stress states in the Nankai subduction zone, southwest Japan, we review various stress-related investigations carried out in the first and second stage expeditions of the Nankai Trough Seismogenic Zone Experiment (NanTroSEIZE) by the Integrated Ocean Drilling Program (IODP) and compile the stress data. Overall, the maximum principal stress sigma(l) in the shallower levels (<similar to 1 km) is vertical from near the center of forearc basin to near the trench and; the maximum horizontal stress S-Hmax (interpreted to be the intermediate principal stress sigma(2)) is generally parallel to the plate convergence vector. The exception to this generalization occurs along the shelf edge of the Nankai margin where S-Hmax, is along strike rather than parallel to the plate convergence vector. Reorientation of the principal stresses at deeper levels (e.g., >similar to 1 km below seafloor or in underlying accretionary prism) with sigma(1) becoming horizontal is also suggested at all deeper drilling sites. We also make a comparison of the stress state in the hanging wall of the frontal plate-interface between Site C0066 in the Nankai and Site C0019 in the Japan Trench subduction zone drilled after the 2011 Mw 9.0 Tohoku-Oki earthquake. In the Japan Trench, a comparison between stress state before and after the 2011 mega-earthquake shows that the stress changed from compression before the earthquake to extension after the earthquake. As a result of the comparison between the Nankai Trough and Japan Trench, a similar current stress state with trench parallel extension was recognized at both C0006 and C0019 sites. Hypothetically, this may indicate that in Nankai Trough it is still in an early stage of the interseismic cycle of a great earthquake which occurs on the decollement and propagates to the toe (around site C0006). (C) 2015 Elsevier B.V. All rights reserved.</P>

Study of the dry methane reforming process using a rotating gliding arc reactor

Wu, A.,Yan, J.,Zhang, H.,Zhang, M.,Du, C.,Li, X. Pergamon Press 2014 International journal of hydrogen energy Vol.39 No.31

Dry methane reforming (DMR) via rotating gliding arc (RGA) discharge, co-driven by a magnetic field and tangential flow, was investigated in this study. Optical emission spectroscopy (OES) was used to characterize the major active species (energetic electrons, radicals, ions, atoms and excited molecules) in the DMR chemical process. The influence of the operational conditions (applied voltage and CH<SUB>4</SUB>/CO<SUB>2</SUB> ratio) on the basic spectroscopic parameters (electron excitation temperature, electron density and rotational temperature) was determined by spectroscopic methods. The rotational and electron excitation temperatures were approximately 1100-1200 K and 1.1-1.7 eV, respectively, indicating the non-thermal equilibrium characteristics of the RGA discharge. The electron density was approximately 5-20 x 10<SUP>21</SUP> m<SUP>-3</SUP> by fitting the line shape of H<SUB>α</SUB> at 656 nm. The conversions of the reactants (CH<SUB>4</SUB> and CO<SUB>2</SUB>) and the selectivities of the products (H<SUB>2</SUB>, CO and C<SUB>2</SUB> hydrocarbon) were analyzed using a gas chromatograph (GC) under different energy inputs or feed gas proportions. The structure and morphology of carbon black produced during the chemical process was characterized by high-resolution transmission electron microscopy (HRTEM) and Raman spectroscopy, indicating the properties of electrical conductivity and high absorption capacity that can be useful for potential application.

Functional effects of β₃-adrenoceptor on pacemaker activity in interstitial cells of Cajal from the mouse colon

Wu, M.J.,Shin, D.H.,Kim, M.Y.,Park, C.G.,Kim, Y.D.,Lee, J.,Park, I.K.,Choi, S.,So, I.,Park, J.S.,Jun, J.Y. North-Holland ; Elsevier Science Ltd 2015 european journal of pharmacology Vol.754 No.-

<P>We investigated the presence of beta(3)-adrenoceptor and its functional effects on pacemaker potentials in colonic interstitial cells of Cajal (ICCs) from mice. The whole cell patch clamp technique was used to record pacemaker potentials in cultured ICCs and reverse transcription polymerase chain reaction (RT-PCR) was performed to detect the mRNA transcript levels beta-adrenoceptors. The beta(3)-adrenoceptor agonist, BRL37344, reduced the frequency of pacemaker potentials in a Concentration dependent manner. The inhibitory effects of BRL37344 wore blocked by the pretreatment of propranolol, a nonspecific beta-adrenoceptor antagonist, but not by the selective beta(1)-adrenoceptor antagonist atenolol and the selective beta(2)-adrenoceptor antagonist butoxamine. beta(3)-adrenocepto antagonists SR59230A and L748337 blocked the inhibitory effects of BRL37344. RT-PCR revealed mRNA transcripts of beta(1)- and beta(3)-adrenoceptor, but not beta(2)-adrenoceptor, in c-kit- and Ano-1-positive colonic ICCs. The K+ channel blockers tetraethylammoniu, apamin, and glibenclamide did not block the effects of BRL37344. N-omega-Nitio-L-arginiue methyl ester hydrochloride (L-NAME), an NO synthase inhibitor, and chelerythrine, a protein Kinase C inhibitor, also did not block the effects of BRL37344. Noradrenaline mimicked the effects of BRL37344 in colonic ICCs. However, the inhibitory effects of noradrenaline on pacemaker potentials were blocked only by pretreatment with atenolol but not by butoxamine, SR59230A, or L748337 in small intestinal ICCs, BRL37344 had no effect On pacemaker potentials and mRNA transcripts of beta(1)-and beta(2)-adrenoceptor, but not beta(3)-adrenoceptor were detected. These results suggest that beta(3)-adrenoceptors are present in colonic ICCs and may play a role in regulating gastrointestinal motility by the inhibition or pacemaker potentials. (C) 2015 Elsevier By. All rights reserved.</P>

Real-time Tumor Oxygenation Changes After Single High-dose Radiation Therapy in Orthotopic and Subcutaneous Lung Cancer in Mice: Clinical Implication for Stereotactic Ablative Radiation Therapy Schedule Optimization

Song, C.,Hong, B.J.,Bok, S.,Lee, C.J.,Kim, Y.E.,Jeon, S.R.,Wu, H.G.,Lee, Y.S.,Cheon, G.J.,Paeng, J.C.,Carlson, D.J.,Kim, H.J.,Ahn, G.O. Pergamon Press 2016 International journal of radiation oncology, biolo Vol.95 No.3

<P>Purpose: To investigate the serial changes of tumor hypoxia in response to single high-dose irradiation by various clinical and preclinical methods to propose an optimal fractionation schedule for stereotactic ablative radiation therapy. Methods and Materials: Syngeneic Lewis lung carcinomas were grown either orthotopically or subcutaneously in C57BL/6 mice and irradiated with a single dose of 15 Gy to mimic stereotactic ablative radiation therapy used in the clinic. Serial [F-18]-misonidazole (F-MISO) positron emission tomography (PET) imaging, pimonidazole fluorescence-activated cell sorting analyses, hypoxia-responsive element-driven bioluminescence, and Hoechst 33342 perfusion were performed before irradiation (day -1), at 6 hours (day 0), and 2 (day 2) and 6 (day 6) days after irradiation for both subcutaneous and orthotopic lung tumors. For F-MISO, the tumor/brain ratio was analyzed. Results: Hypoxic signals were too low to quantitate for orthotopic tumors using F-MISO PET or hypoxia-responsive element-driven bioluminescence imaging. In subcutaneous tumors, the maximum tumor/brain ratio was 2.87 +/- 0.483 at day -1, 1.67 +/- 0.116 at day 0, 2.92 +/- 0.334 at day 2, and 2.13 +/- 0.385 at day 6, indicating that tumor hypoxia was decreased immediately after irradiation and had returned to the pretreatment levels at day 2, followed by a slight decrease by day 6 after radiation. Pimonidazole analysis also revealed similar patterns. Using Hoechst 33342 vascular perfusion dye, CD31, and cleaved caspase 3 coimmunostaining, we found a rapid and transient vascular collapse, which might have resulted in poor intratumor perfusion of F-MISO PET tracer or pimonidazole delivered at day 0, leading to decreased hypoxic signals at day 0 by PET or pimonidazole analyses. Conclusions: We found tumor hypoxia levels decreased immediately after delivery of a single dose of 15 Gy and had returned to the pretreatment levels 2 days after irradiation and had decreased slightly by day 6. Our results indicate that single high-dose irradiation can produce a rapid, but reversible, vascular collapse in tumors. (C) 2016 Elsevier Inc. All rights reserved.</P>

Astaxanthin reduces hepatic lipid accumulations in high-fat-fed C57BL/6J mice via activation of peroxisome proliferator-activated receptor (PPAR) alpha and inhibition of PPAR gamma and Akt

Jia, Y.,Wu, C.,Kim, J.,Kim, B.,Lee, S.J. Butterworths ; Elsevier Science Ltd 2016 The Journal of nutritional biochemistry Vol.28 No.-

<P>We have previously reported that astaxanthin (AX), a dietary carotenoid, directly interacts with peroxisome proliferator-activated receptors PPAR alpha and PPAR gamma, activating PPAR alpha while inhibiting PPAR gamma, and thus reduces lipid accumulation in hepatocytes in vitro. To investigate the effects of AX in vivo, high-fat diet (HFD)-fed C57BL/6J mice were orally administered AX (6 or 30 mg/kg body weight) or vehicle for 8 weeks. AX significantly reduced the levels of triglyceride both in plasma and in liver compared with the control HFD mice. AX significantly improved liver histology and thus reduced both steatosis and inflammation scores of livers with hematoxylin and eosin staining. The number of inflammatory macrophages and Kupffer cells were reduced in livers by AX administration assessed with F4/80 staining. Hepatic PPAR alpha-responsive genes involved in fatty acid uptake and beta-oxidation were upregulated, whereas inflammatory genes were downregulated by AX administration. In vitro radiolabeled assays revealed that hepatic fatty acid oxidation was induced by AX administration, whereas fatty acid synthesis was not changed in hepatocytes. In mechanism studies, AX inhibited Akt activity and thus decreased SREBP1 phosphorylation and induced Insig-2a expression, both of which delayed nuclear translocation of SREBP1 and subsequent hepatic lipogenesis. Additionally, inhibition of the Akt-mTORC1 signaling axis by AX stimulated hepatic autophagy that could promote degradation of lipid droplets. These suggest that AX lowers hepatic lipid accumulation in HFD-fed mice via multiple mechanisms. In addition to the previously reported differential regulation of PPAR alpha and PPAR gamma, inhibition of Akt activity and activation of hepatic autophagy reduced hepatic steatosis in mouse livers. (C) 2015 Elsevier Inc. All rights reserved.</P>

Water as an Oxygen Source: Synthesis, Characterization, and Reactivity Studies of a Mononuclear Nonheme Manganese(IV) Oxo Complex

Sawant, Sarvesh C.,Wu, Xiujuan,Cho, Jaeheung,Cho, Kyung‐,Bin,Kim, Sun Hee,Seo, Mi Sook,Lee, Yong‐,Min,Kubo, Minoru,Ogura, Takashi,Shaik, Sason,Nam, Wonwoo WILEY‐VCH Verlag 2010 Angewandte Chemie Vol.122 No.44

<P><B>Kontrollierte Herkunft</B>: Experimente mit isotopenmarkiertem Wasser lassen keinen Zweifel an der Quelle von Sauerstoff in einem Nicht‐Häm‐Mangan(IV)‐Oxo‐Komplex. Der mit Wasser als Sauerstoffquelle und einem Cer(IV)‐Oxidationsmittel gebildete Komplex (siehe Bild) aktiviert C‐H‐Bindungen von Alkylarenen und oxidiert aromatische Substrate (Sub) sowie Benzylalkohol.</P>

The 14-3-3 Gene Function of Cryptococcus neoformans Is Required for its Growth and Virulence

( Jingbo Li ),( Yun C. Chang ),( Chun Hua Wu ),( Jennifer Liu ),( Kyung J. Kwon Chung ),( Sheng He Huang ),( Hiro Shimada ),( Rob Fante ),( Xiaowei Fu ),( Ambrose Jong ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.5

Cryptococcus neoformans is a life-threatening pathogenic yeast that causes devastating meningoencephalitis. The mechanism of cryptococcal brain invasion is largely unknown, and recent studies suggest that its extracellular microvesicles may be involved in the invasion process. The 14-3-3 protein is abundant in the extracellular microvesicles of C. neoformans, and the 14-3-3-GFP fusion has been used as the microvesicle’s marker. However, the physiological role of 14-3-3 has not been explored. In this report, we have found that C. neoformans contains a single 14-3-3 gene that apparently is an essential gene. To explore the functions of 14-3-3, we substituted the promoter region of the 14-3-3 with the copper-controllable promoter CTR4. The CTR4 regulatory strain showed an enlarged cell size, drastic changes in morphology, and a decrease in the thickness of the capsule under copper-enriched conditions. Furthermore, the mutant cells produced a lower amount of total proteins in their extracellular microvesicles and reduced adhesion to human brain microvascular endothelial cells in vitro. Proteomic analyses of the protein components under 14-3-3-overexpressed and -suppressed conditions revealed that the 14-3-3 function(s) might be associated with the microvesicle biogenesis. Our results support that 14-3-3 has diverse pertinent roles in both physiology and pathogenesis in C. neoformans. Its gene functions are closely relevant to the pathogenesis of this fungus.

Optical Characterization of ZnMnO Thin Films on c-Al2O3

H. J. LIN,D. Y. Lin,J. S. Wu,W. C. CHOU,C. S. YANG,J. S. WANG,W. H. Lo 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.1

Various optical measurement technologies have ben used to characterize ZnMnO thin filmswith different Mn compositions grown by molecular beam epitaxy(MBE) on c-Al2O3 substrates. Thelat- tice constant and the crystalization quality have ben evaluated by using X-ray diraction (XRD). Photoluminescence (PL) has ben used to reveal the neutral-donor-bound exciton (D0X) and to checkthelm'squality. Defect-relatedabsorptionsignatures,inadditiontonear-band-edgeabsorp- tion, due to the zinc vacancy and the donor-aceptor pair (DAP) have ben found in the surface photovoltagespectra(SPS).Freexcitonictransitionsandtheirphonon-asistedreplicashaveben observed in the re ectance spectra. Our experimental results not only unveil specic optical tran- sitionenergies but also indicate arapid materiald deterioration whenMn incorporation goes beyond a certain amount to cause manganese segregation. Various optical measurement technologies have ben used to characterize ZnMnO thin filmswith different Mn compositions grown by molecular beam epitaxy(MBE) on c-Al2O3 substrates. Thelat- tice constant and the crystalization quality have ben evaluated by using X-ray diraction (XRD). Photoluminescence (PL) has ben used to reveal the neutral-donor-bound exciton (D0X) and to checkthelm'squality. Defect-relatedabsorptionsignatures,inadditiontonear-band-edgeabsorp- tion, due to the zinc vacancy and the donor-aceptor pair (DAP) have ben found in the surface photovoltagespectra(SPS).Freexcitonictransitionsandtheirphonon-asistedreplicashaveben observed in the re ectance spectra. Our experimental results not only unveil specic optical tran- sitionenergies but also indicate arapid materiald deterioration whenMn incorporation goes beyond a certain amount to cause manganese segregation.