Inhibitory Effect of Melanoma Differentiation Associated Gene-7/Interleukin-24 on Invasion In Vitro of Human Melanoma Cancer Cells

Bi-wen Lin,Ze-long Jiao,Jian-feng Fan,Liang Peng,Lei Li,Zi-gang Zhao,Xiang-yu Ding,이형진 대한의학회 2013 Journal of Korean medical science Vol.28 No.6

The acquisition of metastasis potential is a critical point for malignant tumors. Melanoma differentiation associated gene-7/interleukin-24 (mda-7/IL-24) is a potential tumor suppress gene and frequently down-regulated in malignant tumors. It has been implicated that overexpression of MDA-7 led to proliferation inhibition in many types of human tumor. Invasion is an important process which is potential to promote tumor metastasis. However, the role and potential molecular mechanism of mda-7/IL-24 to inhibit the invasion of human melanoma cancer is not fully clear. In this report, we identified a solid role for mda-7/IL-24 in invasion inhibition of human melanoma cancer LiBr cells, including decreasing of adhesion and invasion in vitro, blocking cell cycle, down-regulating the expression of ICAM-1, MMP-2/9, CDK1, the phosphorylation of ERK and Akt, NF-κB and AP-1 transcription activity. Meanwhile, there was an increased expression of PTEN in mda-7/IL-24 over-expression LiBr cells. Our results demonstrated that mda-7/IL-24 is a potential invasion suppress gene, which inhibits the invasion of LiBr cells by the down-regulation of ICAM-1, MMP-2/9, PTEN, and CDK1 expression. The molecular pathways involved were the MAPK/ERK, PI3K-Akt, NF-κB, and AP-1. These findings suggest that mda-7/IL-24 may be used as a possible therapeutic strategy for human melanoma cancer.

Dynamic Function Allocation Method for Aircraft Cockpit Based on Operator Functional State

Wen-hao Bi,An Zhang,Shui-da Bao 제어로봇시스템학회 2015 제어로봇시스템학회 국제학술대회 논문집 Vol.2015 No.10

Dynamic function allocation for aircraft cockpit benefits the complementary between operators and automation and the whole system performance. We selected two important physiological parameters HRV2 and TLI2, which have great influences on the operator functional state (OFS) and took their measurements as a trigger mechanism for dynamic function allocation of the cockpit system. After that, we utilized fuzzy logic to put forward a dynamic adjustment methodology for the levels of automation and used MATLAB to verify the utility of the method.

Effect of microwave irradiation on cellular disintegration of Gram positive and negative cells

Zhou, Bi Wen,Shin, Seung Gu,Hwang, KwangHyun,Ahn, Johng-Hwa,Hwang, Seokhwan Springer-Verlag 2010 Applied microbiology and biotechnology Vol.87 No.2

Characteristics, Prognostic Factors, and Survival of Patients with NK/T-Cell Lymphoma of Non-upper Aerodigestive Tract: A 17-Year Single-Center Experience

Ze-Long Liu,Xi-Wen Bi,Xue-Wen Zhang,De-Xin Lei,Pan-Pan Liu,Hang Yang,Yan Gao,Yuan-Xue Jiang,Wen-Qi Jiang,Yi Xia 대한암학회 2019 Cancer Research and Treatment Vol.51 No.4

Purpose The extranodal natural killer (NK)/T-cell lymphoma (NKTCL) of non-upper aerodigestive tract (NUAT) was found to have clinical heterogeneity compared with NKTCL of the upper aerodigestive tract (UAT) in small scale studies. We conducted this study in a much larger cohort to analyze the clinical characteristics, prognostic factors, treatment modality, and clinical outcomes of patients with NUAT-NKTCL. Materials and Methods From January 2001 to December 2017, a total of 757 NKTCL patients were identified and included in this study, including 92 NUAT-NKTCL patients (12.2%) and 665 UAT-NKTCL patients (87.8%). Results NUAT-NKTCL patients had relatively poorer performance status, more unfavorable prognostic factors, and more advanced stage, compared with UAT-NKTCL patients. The 5-year overall survival (OS) was 34.7% for NUAT-NKTCL, which was significantly worse than UAT-NKTCL (64.2%, p < 0.001). The median OS duration was 30.9 months for NUAT-NKTCL. Multivariate analysis showed that presence with B symptoms and elevated serum lactate dehydrogenase independently predicted worse OS. International prognostic index score and prognostic index of NK lymphoma score still had prognostic values in NUAT-NKTCL, while the Ann Arbor system could not accurately predict the OS. Conclusion NUAT-NKTCL is a distinctive subtype of NKTCL in many aspects. Patients with NUAT-NKTCL have relatively poorer performance status, more unfavorable prognostic factors, more advanced stage, and poorer prognosis.

Diversity of Thermophilic Fungi in Tengchong Rehai National Park Revealed by ITS Nucleotide Sequence Analyses

Wen-Zheng Pan,Xiao-Wei Huang,Kang-Bi Wei,Chun-Mei Zhang,Dong-Mei Yang,Jun-Mei Ding,Ke-Qin Zhang 한국미생물학회 2010 The journal of microbiology Vol.48 No.2

The geothermal sites near neutral and alkalescent thermal springs in Tengchong Rehai National Park were examined through cultivation-dependent approach to determine the diversity of thermophilic fungi in these environments. Here, we collected soils samples in this area, plated on agar media conducive for fungal growth, obtained pure cultures, and then employed the method of internal transcribed spacer (ITS)sequencing combined with morphological analysis for identification of thermophilic fungi to the species level. In total, 102 strains were isolated and identified as Rhizomucor miehei, Chaetomium sp., Talaromyces thermophilus, Talaromyces byssochlamydoides, Thermoascus aurantiacus Miehe var. levisporus, Thermomyces lanuginosus, Scytalidium thermophilum, Malbranchea flava, Myceliophthora sp. 1, Myceliophthora sp. 2,Myceliophthora sp. 3, and Coprinopsis sp. Two species, T. lanuginosus and S. thermophilum were the dominant species, representing 34.78% and 28.26% of the sample, respectively. Our results indicated a greater diversity of thermophilic fungi in neutral and alkaline geothermal sites than acidic sites around hot springs reported in previous studies. Most of our strains thrived at alkaline growth conditions.

Apply the Combination of Anthropometric Health Indicators and Sheldon`s Somatotype in Health Promotion

( Wen Ko Chiou ),( Ming Hsu Wang ),( Bi Hui Chen ) 한국감성과학회 2014 춘계학술대회 Vol.2014 No.-

The concept of somatotyping is a unique method for the classification of human physique that was first invented by Sheldon and modified by Heath and Carter. Somatotype is also widely used as a tool to estimate the nutritional status of populations and to monitor the growth and diseases of individuals. Human bodies compose from many parts and it is difficult to judge the healthy status from the single index. The body image compose form the multiple indexes could help people to judge the healthy status from the anthropometry data. The aim of this study was to develop body image templates in both genders from the 3D anthropometry data to evaluate the human healthy status. This study collected the dimensions from anthropometry and body image among 90 Taiwanese participants in the Keelung Chang Gung Memorial Hospital, Taiwan. This research uses the 3D whole body photography system to collect the anthropometry dimensions and analyze the data from the Beauty3D software. Collecting the 5 indexes, including body mass index (BMI), waist circumference (WC), waist-hip ratio (WHR), waist-height ratio (WHtR) and waist-thigh ratio (WTR) and to judge the indexes for normal and abnormal status by the WHO standards.

Full-scale testing and modeling of the mechanical behavior of shield TBM tunnel joints

Wen-qi Ding,Yi-cheng Peng,Zhi-guo Yan,Bi-wei Shen,He-hua Zhu,Xin-xin Wei 국제구조공학회 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.45 No.3

For shield TBM (Tunnel Boring Machine) tunnel lining, the segment joint is the most critical component for determining the mechanical response of the complete lining ring. To investigate the mechanical behavior of the segment joint in a water conveyance tunnel, which is different from the vehicle tunnel because of the external loads and the high internal water pressure during the tunnel’s service life, full-scale joint tests were conducted. The main advantage of the joint tests over previous ones was the definiteness of the loads applied to the joints using a unique testing facility and the acquisition of the mechanical behavior of actual joints. Furthermore, based on the test results and the theoretical analysis, a mechanical model of segment joints has been proposed, which consists of all important influencing factors,including the elastic-plastic behavior of concrete, the pre-tightening force of the bolts and the deformations of all joint components, i.e., concrete blocks, bolts and cast iron panels. Finally, the proposed mechanical model of segment joints has been verified by the aforementioned full-scale joint tests.

Apply the combination of anthropometric health indicators and Sheldon`s somatotype in health promotion

Wen-Ko Chiou,Ming-Hsu Wang,Bi-Hui Chen 대한인간공학회 2014 대한인간공학회 학술대회논문집 Vol.2014 No.5

The concept of somatotyping is a unique method for the classification of human physique that was first invented by Sheldon and modified by Heath and Carter. Somatotype is also widely used as a tool to estimate the nutritional status of populations and to monitor the growth and diseases of individuals. Human bodies compose from many parts and it is difficult to judge the healthy status from the single index. The body image compose form the multiple indexes could help people to judge the healthy status from the anthropometry data. The aim of this study was to develop body image templates in both genders from the 3D anthropometry data to evaluate the human healthy status. This study collected the dimensions from anthropometry and body image among 90 Taiwanese participants in the Keelung Chang Gung Memorial Hospital, Taiwan. This research uses the 3D whole body photography system to collect the anthropometry dimensions and analyze the data from the Beauty3D software. Collecting the 5 indexes, including body mass index (BMI), waist circumference (WC), waist-hip ratio (WHR), waist-height ratio (WHtR) and waist-thigh ratio (WTR) and to judge the indexes for normal and abnormal status by the WHO standards. Using the multiple indexes to category the body health into 4 levels. The results show that 4 body image templates in both genders were created, and the difference between the different templates and genders were discovered. The body image templates showed the human body images come from the different health status. It can be concluded that different body image templates from the multiple dimensions can lead to judge the human healthy status and base on the different templates could provide the visual judgment for the different body types for the health status.

Comparison of Digestive Function Among Rabbits, Guinea-Pigs, Rats and Hamsters. II. Digestive Enzymes and Hindgut Fermentation

Yu, Bi,Chiou, Peter Wen-Shyg,Kuo, Chung-Yi Asian Australasian Association of Animal Productio 2000 Animal Bioscience Vol.13 No.11

The aim of this trial was to study the response of laboratory animals including omnivores (rats) and herbivores (rabbits, guinea pigs and hamsters) to the same level of dietary fiber on their digestive enzymes and hindgut fermentation. Ten weanling animals of each of four species, rabbits, guinea-pigs, rats and Syrian hamster, were fed a basal diet of 18% crude protein and 10% crude fiber for six weeks. The digesta and tissue of each intestinal segment were collected to measure the activity of digestive enzymes. Rabbits contained the highest secreted pepsin activity in the stomach, whereas rats contained the highest protease and ${\alpha}-amylase$ activity in the small intestine, and lower fibrous hydrolases in the hindgut than rabbits, guinea pigs and hamsters. The total VFA productions in the caecum and colon were highest in rats, followed by hamsters and rabbits, while the guinea pigs contained the lowest VFA and a different pattern of VFA molar ratio from the other laboratory animals. The degree of hindgut fermentation in these laboratory animals was in reverse to the trend for their fiber digestion.

Whole Brain Radiotherapy Plus Chemotherapy in the Treatment of Brain Metastases from Lung Cancer: A Meta-analysis of 19 Randomized Controlled Trails

Liu, Wen-Jing,Zeng, Xian-Tao,Qin, Hai-Feng,Gao, Hong-Jun,Bi, Wei-Jing,Liu, Xiao-Qing Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

Objective: To evaluate the efficacy and safety of whole brain radiotherapy (WBRT) plus chemotherapy versus WBRT alone for treating brain metastases (BM) from lung cancer by performing a meta-analysis based on randomized controlled trials (RCTs). Methods: The PubMed, Embase, CENTRAL, ASCO, ESMO, CBM, CNKI, and VIP databases were searched for relevant RCTs performed between January 2000 and March 2012. After quality assessment and data extraction, the meta-analysis was performed using the RevMan 5.1 software, with funnel plot evaluation of publication bias. Results: 19 RCTs involving 1,343 patients were included. The meta-analyses demonstrated that compared to WBRT alone, WBRT plus chemotherapy was more effective with regard to the objective response rate (OR = 2.30, 95% CI = 1.79 - 2.98; P < 0.001); however, the incidences of gastrointestinal reactions (RR = 3.82, 95% CI = 2.33 - 6.28, P <0.001), bone marrow suppression (RR = 5.49, 95% CI = 3.65 - 8.25, P < 0.001), thrombocytopenia (RR = 5.83, 95% CI = 0.39 - 86.59; P = 0.20), leukopenia (RR = 3.13, 95% CI = 1.77 - 5.51; P < 0.001), and neutropenia (RR = 2.75, 95% CI = 1.61 - 4.68; P < 0.001) in patients treated with WBRT plus chemotherapy were higher than with WBRT alone. There was no obvious publication bias detected. Conclusion: WBRT plus chemotherapy can obviously improve total efficacy rate, butalso increases the incidence of adverse reactions compared to WBRT alone. From the limitations of this study, more large-scale, high-quality RCTs are suggested for further verification.