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      • Detection and Identification of Defects in Transparent Film

        Bao-yuan Chen,Chao Zheng,Zhong-xiang Sun,Xiao-yang Yu 보안공학연구지원센터 2015 International Journal of Future Generation Communi Vol.8 No.4

        Biaxially oriented polyester film (BOPET) defect is an important factor affecting the quality of the film. In view of identification of defects in the conventional film production process, this mathod resulted in the identification of defects inaccurate. and low labor efficiency and machine vision recognition on identification of specific defect. This paper presents a LVQ neural network-based BOPET film of defects detection and identification methods. In this algorithm, the film images were processed and the outlines of the membrane defects were obtained. Through extracting the aspect ration, circularity, complexity and elongation , projection histogram central moment and so on, the characteristic values of membrane defects, which from the image of film images after image processing, and then input to the defects recognition system based on LVQ neural network that had been trained, in order to achieve the film defects identification, classification and localization. Through the study of features of the defects in BOPET and extracted some quantities as character input of the LVQ neural network, then input some characteristic values as training value into the LVQ neural network to achieve the learning and prediction purpose, and the LVQ neural network was designed. The experiments show that, the proposed method can meet the requirements analysis of air defects in transparent film.

      • Voice Activity Detection Algorithm based on Improved Radial Basis Function Neural Network

        Bao-yuan Chen,Ya-qiong Lan,Jing-yang Liu,Zi-he Li,Xiao-yang Yu 보안공학연구지원센터 2014 International Journal of Signal Processing, Image Vol.7 No.5

        Voice activity detection (VAD) is the key of voice recognition, voice synthesis and speech-sound enhancement.For the sake of improve the accuracy and robustness of speech endpoint detection system. Combining the advantages of adaptive genetic algorithm (AGA) and improved radial basis function network (RBF) defects in existing learning methods. This paper presents a comprehensive detection method-- Adaptive genetic algorithm radial basis function network. This method uses adaptive genetic algorithm to simultaneously optimize the center, the width and the structure of RBF network. The method using wavelet analysis to extract the characteristics of the speech signal, use them as an input amount to the radial basis function networks. Establish voice detection system model, this method enhance the accuracy of the detection system and has better robustness.

      • KCI등재

        Space Charge Behavior of Oil-paper Insulation Thermally Aged under Different Temperatures and Moistures

        Yuan-Xiang Zhou,Meng Huang,Wei-Jiang Chen,Fu-Bao Jin 대한전기학회 2015 Journal of Electrical Engineering & Technology Vol.10 No.3

        Moisture and high temperature are the most important factors that lead to the ageing of oil-paper insulation, but the research about space charge characteristics of oil-paper insulation does not take the combined effect of ambient temperature, moisture and thermal ageing into account. The pulsed electroacoustic (PEA) method was used to investigate the influence of moisture and temperature on space charge characteristics of oil paper at different ageing stages. The results showed that moisture could speed up formation of space charge in oil paper when water concentration was low, but the formation was restrained if the water concentration was high. At the beginning of thermal ageing, heterogeneous charge accumulation had predominance, but it gradually changed to homogeneous charge injection with ageing. It was believed that moisture concentration could speed up ageing and enhance charge accumulation on one hand, and accelerate or slow down the establishment speed of space charge on the other hand, therefore, charge accumulation type changed with ageing. The more seriously the oil-paper insulation was thermally aged, the deeper the trap energy level was, hence more space charge was trapped, which could be speeded up by increasing the ageing temperature, but the effect of ambient temperature did not fit the Arrhenius law.

      • SCIESCOPUSKCI등재

        Space Charge Behavior of Oil-paper Insulation Thermally Aged under Different Temperatures and Moistures

        Zhou, Yuan-Xiang,Huang, Meng,Chen, Wei-Jiang,Jin, Fu-Bao The Korean Institute of Electrical Engineers 2015 Journal of Electrical Engineering & Technology Vol.10 No.3

        Moisture and high temperature are the most important factors that lead to the ageing of oil-paper insulation, but the research about space charge characteristics of oil-paper insulation does not take the combined effect of ambient temperature, moisture and thermal ageing into account. The pulsed electroacoustic (PEA) method was used to investigate the influence of moisture and temperature on space charge characteristics of oil paper at different ageing stages. The results showed that moisture could speed up formation of space charge in oil paper when water concentration was low, but the formation was restrained if the water concentration was high. At the beginning of thermal ageing, heterogeneous charge accumulation had predominance, but it gradually changed to homogeneous charge injection with ageing. It was believed that moisture concentration could speed up ageing and enhance charge accumulation on one hand, and accelerate or slow down the establishment speed of space charge on the other hand, therefore, charge accumulation type changed with ageing. The more seriously the oil-paper insulation was thermally aged, the deeper the trap energy level was, hence more space charge was trapped, which could be speeded up by increasing the ageing temperature, but the effect of ambient temperature did not fit the Arrhenius law.

      • IMPACTS OF SHELLFISH CULTURE ON THE ECOSYSTEM IN SEMI-ENCLOSED BAYS

        Ming Yuan Zhu,Ru Bao Ji,Shang Chen,Xue Lei Zhang 한국해안해양공학회 1999 학술강연회 발표논문초록집 Vol.1 No.1

        Stimulated by high economic profits, the industries of shellfish culture have grown rapidly during the last two decades. The expansion of mariculture adds pressure on the environment and associated ecosystem. The potential impacts of the cultivation include reduction of primary productivity due to the consumption of large quantities of phytoplankton, changes of water current movement resulted from the semi-permeation 'Island' effects of the suspended culture, and production of a large amount of faeces and pseudofaeces which may enhance the importance of benthic ecosystem in geo-biological cycling.

      • KCI등재

        H-형태 양친매성 펜타블록 공중합체의 화학효소적 합성과 자기회합거동 평가

        Peng Chen,Ya Peng Li,Can Jin Li,Xin Lei Meng,Bao Zhang,Ming Zhu,Yan-jing Liu,Jing Yuan Wang 한국고분자학회 2013 폴리머 Vol.37 No.3

        H-shaped amphiphilic pentablock copolymers (PSt)2-b-PCL-b-PEO-b-PCL-b-(PSt)2 was synthesized via chemoenzymatic method by combining enzyme-catalyzed ring-opening polymerization (eROP) of ε-caprolactone (ε-CL) and atom transfer radical polymerization (ATRP) of styrene. By this process, we obtained copolymers with controlled molecular weight and low polydispersity. The structure and composition of the obtained copolymers were characterized by nuclear magnetic resonance (NMR), gel permeation chromatography (GPC) and infrared spectroscopy analysis (IR). The crystallization behavior of the copolymers was analyzed by differential scanning calorimetry (DSC) and X-ray diffraction (XRD). The crystallization behavior of the H-shaped block copolymers demonstrated a PCL dominate crystallization. The self-assembly behavior of the copolymers was investigated in aqueous media. The hydrodynamic diameters of the copolymer micelles in aqueous solution were measured by dynamic light scattering (DLS). The morphology of the copolymer micelles was observed by atomic force microscopy (AFM) and transmission electron microscopy (TEM). The hydrodynamic diameters of spherical micelles declined gradually with the increase of the hydrophobic chain lengths of the copolymers. The critical micelle concentration (CMC) values were determined from fluorescence emission, and it was found that the CMCs decreased with an increase of PSt hydrophobic block lengths.

      • KCI등재

        LincR-PPP2R5C Promotes Th2 Cell Differentiation Through PPP2R5C/PP2A by Forming an RNA–DNA Triplex in Allergic Asthma

        Ji Ningfei,Chen Zhongqi,Wang Zhengxia,Sun Wei,Yuan Qi,Zhang Xijie,Jia Xinyu,Wu Jingjing,Jiang Jingxian,Song Meijuan,Xu Tingting,Liu Yanan,Ma Qiyun,Sun Zhixiao,Bao Yanmin,Zhang Mingshun,Huang Mao 대한천식알레르기학회 2024 Allergy, Asthma & Immunology Research Vol.16 No.1

        Purpose: The roles and mechanisms of long noncoding RNAs (lncRNAs) in T helper 2 (Th2) differentiation from allergic asthma are poorly understood. We aimed to explore a novel lncRNA, LincR-protein phosphatase 2 regulatory subunit B' gamma (PPP2R5C), in Th2 differentiation in a mouse model of asthma. Methods: LincR-PPP2R5C from RNA-seq data of CD4+ T cells of asthma-like mice were validated and confirmed by quantitative reverse transcription polymerase chain reaction, northern blotting, nuclear and cytoplasmic separation, and fluorescence in situ hybridization (FISH). Lentiviruses encoding LincR-PPP2R5C or shRNA were used to overexpress or silence LincR-PPP2R5C in CD4+ T cells. The interactions between LincR-PPP2R5C and PPP2R5C were explored with western blotting, chromatin isolation by RNA purification assay, and fluorescence resonance energy transfer. An ovalbumin-induced acute asthma model in knockout (KO) mice (LincR-PPP2R5C KO, CD4 conditional LincR-PPP2R5C KO) was established to explore the roles of LincR-PPP2R5C in Th2 differentiation. Results: LncR-PPP2R5C was significantly higher in CD4+ T cells from asthmatic mice ex vivo and Th2 cells in vitro. The lentivirus encoding LincR-PPP2R5C suppressed Th1 differentiation; in contrast, the short hairpin RNA (shRNA) lentivirus decreased LincR-PPP2R5C and Th2 differentiation. Mechanistically, LincR-PPP2R5C deficiency suppressed the phosphatase activity of the protein phosphatase 2A (PP2A) holocomplex, resulting in a decline in Th2 differentiation. The formation of an RNA-DNA triplex between LincR-PPP2R5C and the PPP2R5C promoter enhanced PPP2R5C expression and activated PP2A. LincR-PPP2R5C KO and CD4 conditional KO decreased Th2 differentiation, airway hyperresponsiveness and inflammatory responses. Conclusions: LincR-PPP2R5C regulated PPP2R5C expression and PP2A activity by forming an RNA-DNA triplex with the PPP2R5C promoter, leading to Th2 polarization in a mouse model of acute asthma. Our data presented the first definitive evidence of lncRNAs in the regulation of Th2 cells in asthma.

      • KCI등재

        A novel catalyst of Ni–Mn complex oxides supported on cordierite for catalytic oxidation of toluene at low temperature

        Qiong Huang,Zhi-Yuan Zhang,Wen-Jiao Ma,Ying-Wen Chen,She-Min Zhu,Shu-Bao Shen 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.2

        The catalytic combustion of toluene over Ni–Mn mixed complex supported on industrial cordierite was investigated. The catalysts were prepared by the wet impregnation method and characterized by using the Brunauer Emmett Teller (BET), Scanning Electron Microscopy (SEM), X-ray diffraction (XRD),Transmission Electron Microscope (TEM) and X-ray fluorescence (XRF). The catalytic activity toward the complete oxidation of toluene to CO2 and H2O strongly depended on the molar ratio of Ni/Mn, loading amount of Ni–Mn oxides, and calcination temperature. All the results above indicated that the Ni–Mn complex oxide catalyst calcined at 400 8C with 0.5 mol ratio of Ni/Mn, 10 wt.% loading amounts, and showed the highest activity as complete oxidation of toluene.

      • SCIESCOPUSKCI등재

        Betulin Targets Lipin1/2-Meidated P2X7 Receptor as a Therapeutic Approach to Attenuate Lipid Accumulation and Metaflammation

        ( Jia-yi Dou ),( Yu-chen Jiang ),( Zhong-he Hu ),( Kun-chen Yao ),( Ming-hui Yuan ),( Xiao-xue Bao ),( Mei-jie Zhou ),( Yue Liu ),( Zhao-xu Li ),( Li-hua Lian ),( Ji-xing Nan ),( Yan-ling Wu ) 한국응용약물학회 2022 Biomolecules & Therapeutics(구 응용약물학회지) Vol.30 No.3

        The present study focused on the potential mechanism of betulin (BT), a pentacyclic triterpenoid isolated from the bark of white birch (Betula pubescens), against chronic alcohol-induced lipid accumulation and metaflammation. AML-12 and RAW 264.7 cells were administered ethanol (EtOH), lipopolysaccharide (LPS) or BT. Male C57BL/6 mice were fed Lieber-DeCarli liquid diets containing 5% EtOH for 4 weeks, followed by single EtOH gavage on the last day and simultaneous treatment with BT (20 or 50 mg/kg) by oral gavage once per day. In vitro, MTT showed that 0-25 mM EtOH and 0-25 μM BT had no toxic effect on AML-12 cells. BT could regulate sterolregulatory-element-binding protein 1 (SREBP1), lipin1/2, P2X7 receptor (P2X7r) and NOD-like receptor family, pyrin domains-containing protein 3 (NLRP3) expressions again EtOH-stimulation. Oil Red O staining also indicated that BT significantly reduced lipid accumulation in EtOH-stimulated AML-12 cells. Lipin1/2 deficiency indicated that BT might mediate lipin1/2 to regulate SREBP1 and P2X7r expression and further alleviate lipid accumulation and inflammation. In vivo, BT significantly alleviated histopathological changes, reduced serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and triglyceride (TG) levels, and regulated lipin1/2, SREBP1, peroxisome proliferator activated receptor α/γ (PPARα/γ) and PGC-1α expression compared with the EtOH group. BT reduced the secretion of inflammatory factors and blocked the P2X7r-NLRP3 signaling pathway. Collectively, BT attenuated lipid accumulation and metaflammation by regulating the lipin1/2-mediated P2X7r signaling pathway.

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