Identification and expression patterns of 20-hydroxyecdysoneresponsive genes from Procambarus clarkii

Bao‑Jian Zhu,Lin Tang,Ying‑Ying Yu,Dao‑Jun Wang,Chao‑Liang Liu 한국유전학회 2017 Genes & Genomics Vol.39 No.6

Ecdysteroids play an important role in the regulation of molting process in crustaceans. The suppression subtractive hybridization (SSH) technique was employed to search for 20-hydroxyecdysone-responsive genes in the hepatopancreas of the crayfish Procambarus clarkii. Ninety-three putative expressed sequence tags were identified by SSH, including three immune response-related genes, two cell cycle and apoptosis genes, four respiration and energy metabolism genes, four transport-related genes, six metabolism-related genes, two stress response genes, and eight transcription and translation regulation genes. The expression levels of the examined genes were regulated by 20-hydroxyecdysone and varied in the hepatopancreas during the molting stages; data were confirmed by real-time PCR. RNAi of the ecdysteroid receptor had significant effects on the expression levels of 20-hydroxyecdysone- responsive genes. These differentially expressed genes identified by SSH will provide insight into the ecdysteroid signaling pathway in P. clarkii.

Voice Activity Detection Algorithm based on Improved Radial Basis Function Neural Network

Bao-yuan Chen,Ya-qiong Lan,Jing-yang Liu,Zi-he Li,Xiao-yang Yu 보안공학연구지원센터 2014 International Journal of Signal Processing, Image Vol.7 No.5

Voice activity detection (VAD) is the key of voice recognition, voice synthesis and speech-sound enhancement.For the sake of improve the accuracy and robustness of speech endpoint detection system. Combining the advantages of adaptive genetic algorithm (AGA) and improved radial basis function network (RBF) defects in existing learning methods. This paper presents a comprehensive detection method-- Adaptive genetic algorithm radial basis function network. This method uses adaptive genetic algorithm to simultaneously optimize the center, the width and the structure of RBF network. The method using wavelet analysis to extract the characteristics of the speech signal, use them as an input amount to the radial basis function networks. Establish voice detection system model, this method enhance the accuracy of the detection system and has better robustness.

Glucosamine induces cell death $via$ proteasome inhibition in human ALVA41 prostate cancer cell

Liu, Bao-Qin,Meng, Xin,Li, Chao,Gao, Yan-Yan,Li, Ning,Niu, Xiao-Fang,Guan, Yifu,Wang, Hua-Qin Korean Society for Biochemistry and Molecular Bion 2011 Experimental and molecular medicine Vol.43 No.9

Glucosamine, a naturally occurring amino monosaccharide, has been reported to play a role in the regulation of apoptosis more than half century. However the effect of glucosamine on tumor cells and the involved molecular mechanisms have not been thoroughly investigated. Glucosamine enters the hexosamine biosynthetic pathway (HBP) downstream of the rate-limiting step catalyzed by the GFAT (glutamine:fluctose- 6-phosphate amidotransferase), providing UDP-GlcNAc substrates for O-linked ${\beta}$-N-acetylglucosamine (O-GlcNAc) protein modification. Considering that O-GlcNAc modification of proteasome subunits inhibits its activity, we examined whether glucosamine induces growth inhibition $via$ affecting proteasomal activity. In the present study, we found glucosamine inhibited proteasomal activity and the proliferation of ALVA41 prostate cancer cells. The inhibition of proteasomal activity results in the accumulation of ubiquitinated proteins, followed by induction of apoptosis. In addition, we demonstrated that glucosamine downregulated proteasome activator $PA28{\gamma}$ and overexpression of $PA28{\gamma}$ rescued the proteasomal activity and growth inhibition mediated by glucosamine. We further demonstrated that inhibition of O-GlcNAc abrogated $PA28{\gamma}$ suppression induced by glucosamine. These findings suggest that glucosamine may inhibit growth of ALVA41 cancer cells through downregulation of $PA28{\gamma}$ and inhibition of proteasomal activity via O-GlcNAc modification.

现代汉语“V+C1C2+NP处”结构的使用现象研究

Bao Jun Liu,임소정 한국중국어교육학회 2023 중국어교육과연구 Vol.- No.40

In Modern Chinese grammar, it is stipulated that the locative object following a verb can only be placed in between the compound directional complement. However, there is a significant phenomenon of the locative object appearing after the compound directional complement in our surroundings. How is this ungrammatical word order phenomenon distributed and what differences exist between it and the normal grammatical expression? Based on previous research, this article presents a comparative analysis of a large corpus of data, enumerating the distribution and syntactic differences between two types of expressions. For instance, the verb category represented by "进" and "回" tends to use the intermediate-object structure, while the verb category represented by "出" and "过" tends to use the post-object structure in the compound directional complement. From an information structure perspective, the intermediate-object structure is often employed to emphasize the locative object at the end of a sentence, while the post-object structure is frequently used in serial verb constructions to highlight the following constituent. Through an analysis of the corpus and a comparison with actual usage through questionnaire surveys, this article discovers that the phenomenon of placing the locative object after the compound directional complement deserves our attention. It is not simply ungrammatical sentences but rather a special word order with usage patterns and tendencies. Moreover, to some extent, it can fulfill the needs of specific syntax and semantics. 现代汉语语法中规定动词后处所宾语只能用在复合趋向补语的中间使用,但在我们周围存在大量处所宾语用在复合趋向补语后的语序现象。这种不合语法的语序现象是如何分布的且与正常的语法表述之间又存在什么样的差异呢?本文在前人研究的基础上,对语料库中大量语料对比分析列举出两种表述格式的分布情况和语序差异。如“进、回”字类动趋式多用中宾式表述;“出、过”字类动趋式多用后宾式表述。从信息结构来看中宾式多用在句末突出处所宾语,而后宾式多用在连动句中突出后面成分。本文在对语料库分析的同时通过问卷调查与人们实际的使用情况对比发现,处所宾语用在复合趋向补语后的语序现象值得我们关注,其并非单纯的病句,而是有使用规律和倾向性的特殊语序,且在一定程度上能满足特殊句法和语义的需要。

Nitric Oxide Synthase Expressions in ADR-induced Cardiomyopathy in Rats

( Bao Gang Liu ),( Hong Li Li ),( Hong Yan Qu ),( Bao Gui Sun ) 생화학분자생물학회 2006 BMB Reports Vol.39 No.6

Gene Microarray Assessment of Multiple Genes and Signal Pathways Involved in Androgen-dependent Prostate Cancer Becoming Androgen Independent

Liu, Jun-Bao,Dai, Chun-Mei,Su, Xiao-Yun,Cao, Lu,Qin, Rui,Kong, Qing-Bo Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

To study the gene expression change and possible signal pathway during androgen-dependent prostate cancer (ADPC) becoming androgen-independent prostate cancer (AIPC), an LNCaP cell model of AIPC was established using flutamide in combination with androgen-free environment inducement, and differential expression genes were screened by microarray. Then the biological process, molecular function and KEGG pathway of differential expression genes are analyzed by Molecule Annotation System (MAS). By comparison of 12,207 expression genes, 347 expression genes were acquired, of which 156 were up-ragulated and 191 down-regulated. After analyzing the biological process and molecule function of differential expression genes, these genes are found to play crucial roles in cell proliferation, differntiation, cell cycle control, protein metabolism and modification and other biological process, serve as signal molecules, enzymes, peptide hormones, cytokines, cytoskeletal proteins and adhesion molecules. The analysis of KEGG show that the relevant genes of AIPC transformation participate in glutathione metabolism, cell cycle, P53 signal pathway, cytochrome P450 metabolism, Hedgehog signal pathway, MAPK signal pathway, adipocytokines signal pathway, PPAR signal pathway, TGF-${\beta}$ signal pathway and JAK-STAT signal pathway. In conclusion, during the process of ADPC becoming AIPC, it is not only one specific gene or pathway, but multiple genes and pathways that change. The findings above lay the foundation for study of AIPC mechanism and development of AIPC targeting drugs.

Eigenvalue problems of rotor system with uncertain parameters

Bao-Guo Liu 대한기계학회 2012 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.26 No.1

A general method for investigating the eigenvalue problems of a rotor system with uncertain parameters is presented in this paper. The recurrence perturbation formulas based on the Riccati transfer matrix method are derived and used for calculating the first- and secondorder perturbations of eigenvalues and their respective eigenvectors for the rotor system with uncertain parameters. In addition, these formulas can be used for investigating the independent, and repeated, as well as the complex eigenvalue problems. The general method is called the Riccati perturbation transfer matrix method (Riccati-PTMM). The formulas for calculating the mean value, variance, and covariance of the eigenvalues and eigenvectors of the rotor system with random parameters are also given. Riccati-PTMM is used for calculating the random eigenvalues of a simply supported Timoshenko beam and a test rotor supported by two oil bearings. The results show that the method is accurate and efficient.

Glucosamine induces cell death via proteasome inhibition in human ALVA41 prostate cancer cell

Bao-Qin Liu,Hua-Qin Wang,Xin Meng,Chao Li,Yan-Yan Gao,Ning Li,Xiao-Fang Niu,Yifu Guan 생화학분자생물학회 2011 Experimental and molecular medicine Vol.43 No.9

Glucosamine, a naturally occurring amino monosaccharide,has been reported to play a role in the regulation of apoptosis more than half century. However the effect of glucosamine on tumor cells and the involved molecular mechanisms have not been thoroughly investigated. Glucosamine enters the hexosamine biosynthetic pathway (HBP) downstream of the rate-limiting step catalyzed by the GFAT (glutamine:fluctose-6-phosphate amidotransferase), providing UDPGlcNAc substrates for O-linked β-N-acetylglucosamine (O-GlcNAc) protein modification. Considering that O-GlcNAc modification of proteasome subunits inhibits its activity, we examined whether glucosamine induces growth inhibition via affecting proteasomal activity. In the present study, we found glucosamine inhibited proteasomal activity and the proliferation of ALVA41 prostate cancer cells. The inhibition of proteasomal activity results in the accumulation of ubiquitinated proteins, followed by induction of apoptosis. In addition, we demonstrated that glucosamine downregulated proteasome activator PA28γ and overexpression of PA28γ rescued the proteasomal activity and growth inhibition mediated by glucosamine. We further demonstrated that inhibition of O-GlcNAc abrogated PA28γ suppression induced by glucosamine. These findings suggest that glucosamine may inhibit growth of ALVA41 cancer cells through downregulation of PA28γ and inhibition of proteasomal activity via O-GlcNAc modification.

Thermal Aging Characteristics of Ash material and Alkali Metal on SiC-DPF

Bao Liu,Jong-Woo Jeong,Byungchul Choi 한국자동차공학회 2008 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-

The purpose of this study is to verity that H₂O may be a conceived impact factor on SiC-DPF durability which is controlled by hazardous substances collected from the engine exhaust sinters and adheres to the filter wall. Chemical reactions between SiC filter and others, i.e. metal oxide ash and/or alkali metal, were simulated in the extreme circumstance of uncontrolled regeneration in DPF at high temperature by comparing with the results of different conditions (air or air with 10% H₂O), and defined with various characteristics by using the microscopically (SEM-EDS), physicochemical (BET isotherm) and phase analysis (XRD). According to results, H₂O presented condition provide more damage to SiC-DPF durability compared with that of air condition.

Comparative Serum Proteomic Analysis of Serum Diagnosis Proteins of Colorectal Cancer Based on Magnetic Bead Separation and MALDI-TOF Mass Spectrometry

Deng, Bao-Guo,Yao, Jin-Hua,Liu, Qing-Yin,Feng, Xian-Jun,Liu, Dong,Zhao, Li,Tu, Bin,Yang, Fan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Background: At present, the diagnosis of colorectal cancer (CRC) requires a colorectal biopsy which is an invasive procedure. We undertook this pilot study to develop an alternative method and potential new biomarkers for diagnosis, and validated a set of well-integrated tools called ClinProt to investigate the serum peptidome in CRC patients. Methods: Fasting blood samples from 67 patients diagnosed with CRC by histological diagnosis, 55 patients diagnosed with colorectal adenoma by biopsy, and 65 healthy volunteers were collected. Division was into a model construction group and an external validation group randomly. The present work focused on serum proteomic analysis of model construction group by ClinProt Kit combined with mass spectrometry. This approach allowed construction of a peptide pattern able to differentiate the studied populations. An external validation group was used to verify the diagnostic capability of the peptidome pattern blindly. An immunoassay method was used to determine serum CEA of CRC and controls. Results: The results showed 59 differential peptide peaks in CRC, colorectal adenoma and health volunteers. A genetic algorithm was used to set up the classification models. Four of the identified peaks at m/z 797, 810, 4078 and 5343 were used to construct peptidome patterns, achieving an accuracy of 100% (> CEA, P<0.05). Furthermore, the peptidome patterns could differentiate the validation group with high accuracy close to 100%. Conclusions: Our results showed that proteomic analysis of serum with MALDI-TOF MS is a fast and reproducible approach, which may provide a novel approach to screening for CRC.