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거북밀깍지벌레(Cerostegia japonicus)의 發生消長과 그 防除에 관한 硏究
裵泰雄,曺種澤,河祥永,韓熙世,文炳周 東亞大學校 1979 東亞論叢 Vol.16 No.2
거북밀깍지벌레의 效果的인 防除法을 규명하기 위하여 釜山地域에서의 發生消長을 검토하고, 4종류의 寄主別로 기계유유제, 有機燐劑 3종 및 Carbamate劑 3종을 공시하여 殺蟲率을 Bliss의 Probit법으로 분석하고 農藥의 농도에 따른 蟲體의 呼吸代謝를 검토 하였다. 1. 釜山地方에서의 거북밀깍지벌레의 孵化器는 6月15日∼7月12日이였으며 孵化最盛期는 7月 初旬이였다. 2. 防除適期는 8月10日경이었다. 3. 8月3日에 처리한 寄主別의 본 害蟲의 LD?이 가장 낮은 것은 자목련에서는 DEP, 감나무에서는 BUX,사철나무와 치자나무에서는 BUX와 DEP로서 전체적으로 볼 때 BUX 및 DEP가 가장 效果的이고 그 다음이 Diazinon이였다. 4. 8月18日 처리에서 LD?이 가장 낮은 것은 자목련과 감나무에서는 Diazinon이, 사철나무와 치자나무에서는 BUX와 DEP로서 전체적으로 보면 Diazinon, BUX와 DEP등이 感受性이 높았다. 5. 8月3日의 처리에서 LD?이 가장 낮은 농약은 BUX로서 寄主別 농도 범위는 0.011∼0.043%였다. 6. 8月18日의 처리에서 LD?이 가장 낮은 농약은 BUX와 Diazinon으로서 寄主別 농도 범위는 각각 0.011∼0.1076%, 0.028∼0.074%였다. 7. 本 害蟲의 正常 酸素消費量은 9.25∼10.02㎕O₂/㎎ body weight/hour였으며 寄主에 따른 차이를 인정할 수 없었다. 8. Diazinon과 BUX에서는 농도가 높아질수록 산소소비량은 정상호흡량 보다 훨씬 증가하였으나 기계유유제에서는 감소되었다. In order to investigate the effective control of Florida was scale (Cerostegia japonicus), the seasonal occurrences of 1st in star in Busan area was examined, the insecticides tested with the different four hosts were carbamates(NAC,BUX BPMC), organicphosphates(DEP,MEP,Diazinon)and Machine-oil emulson, and the mortality was analyzed by the probit analysis. Also the effects of insecticide's concentration on respiratory metabolism of Florida wax scale was examined. The results obtained were as follows 1. The periods of the larva occurrence was from June 15 to July 12, and the major peak of its occurrence was found to be in the early July. 2. The adequate period for the effective control of Florida wax scale was about August 10. 3. The lowest insecticides of LD 50 values to Florida wax scale with each hosts in August 10 was DEP in the people magnolia, BUX in the persimmon tree, BUX and DEP in the spindle tree and the gardenia. So generally the most effective insecticides for controlling it were BUX and DEP, Diazinon was the next. 4. In the second experiments treated in August 18, the lowest insecticide of LD50 values to it was Diazinon in the purple magnolia and the persimmon tree, BUX and DEP in the spindle tree and the gardenia. So the susceptibility of it to the insecticides was highest in Diazinon, BUX and DEP. 5. The lowest insecticide of LD90 values to it was BUX and the concentration range by the hosts was from. 0.011% to 0.043%. 6. In the second experiment treated in August 18, the lowest insecticides of LD90 values to it were BUX and Diazinon, and the concentration ranges by the hosts were from 0.011 to 0.1076%, 0.028 to 0.074% respectively. 7. The normal oxygen consumption of Florida wax scale was from 9.25 to 10.02㎕O₂/㎎ body weight/hour and wasn't recognized the significance by the hosts. 8. The oxygen consumption of it in Diazinon and BUX was higher with increasing of insecticides concentration than the normal respiratory, but it was decreased in machine oil emulsion.
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人蔘脂溶牲 成分이 四鹽化炭素 投與 白鼠의 蛋白質代謝에 미치는 影響
朴鍾培,柳總根 고려대학교 의과대학 1984 고려대 의대 잡지 Vol.21 No.1
In order to observe a part of the effect of Ginseng fat soluble components (Gx) on the protein metabolism under CCI₄ poisoning, 192 male and female albino rats were taken as subjects which were divided into 6 experimental groups i.e. control grop; CCI₄ group which were observed after CCI₄ and been administered only one time; Gx group which were observed administering Ginseng one time daily; CCI₄+Gx group which were observed administering Ginseng one time daily after CCI₄ had been administered, Gx+CCI₄ group to which CCI₄ was administered only on time after Ginseng had been administered 6 times; and Gx_CCI₄+Gx group to which Ginseng was administered one time daily after CCI₄ had been administered following a 6th time administration of Ginseng. Programed administrations of Ginseng or CCI₄ were given to each corresponding groups at several periods i.e. 1,2,4,6 and 8 days after the intial administration of given doses, changes of total protein, albumin, globulin, urea nitrogen, transaminase (GOT) of serum and total nitrogen observed at each period were measured and the results obtained are as follows: 1. Gx administration was found to have the ability to decrease the elevated SGOT activity resulting from administration of CCI₄ and induce rapid recrovery from such an elevated level and the extent of such a dereasing action was greatest in Gx+CCI₄+Gx group, than Gx+CCI₄ group, and the least in CCI₄+Gx group. 2. Gx+CCI₄+Gx group showed nearly no change in serum albumin level, and being in second place, Gx administering groups (CCI₄+Gx group, Gx+CCI₄ group) showed small change. 3. Gx administering groups showed more increase in globulin level at 1-2 day period than the CCI₄ group and during the same period A/G ratio was found inverted. 4. Total nitrogen amount in the liver was remarkably decreased in Gx administering groups at 2 day period. 5. Urea nitrogen was increased in CCI₄ group at 2 day period, whereas Gx administering groups showed decreases. 6. Great changes in electrophoresis fractions were found in α₁-and r-globulin fractions at 1-2 day period. For more detail a. α₁-globulin; Gx administering groups showed lower increases than the CCI₄ group at 2 day period. b. r-globulin; Gx_CCI₄ group and CCI₄+Gx group showed more increases than the CCI₄ group at 1-2 day period. 7. α₂-globulin, revealing characteristic pattern, was increased to the same extent in all the Gx administerings groups during Gx administering period, thus Gx was estimated to increase the α₂-globulin component.
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李鍾哲,崔震浹,裵啓宣,孫興大 동아대학교 환경문제연구소 1979 硏究報告 Vol.3 No.1
Those studies were examined rearing two varieties on Japanese descent and two varieties of Chinese descent by feeding polluted mulberry leaves, non-polluted ones and water-cleaned ones respectively to find effects air-pollution on the economic characters of silkworms and analysis of contents of Sulphur and Cadmium in the mulberry leaves and silkworms as followings; 1) Japanese descent of polluted part was delayed about 2.5 days than non-polluted part, Chinese descent was delayed about days or inequal and water-cleaned part was medium in the silkworm larval duration. 2) Results of maximum weight of 5th instar, cocoon later weight and cocoon weight were decreased in due order non-polluted, water-cleaned and polluted in the factors of mulberry. 3) Pupal ratio of Japanese descent was not shown statistical significance, but Chinese descent was revealed it obviously in the factors of mulberry leaves. 4) In the resistance of polluted mulberry leaves, Chinese descent was feeble obviously than Japanese and there were some difference even through among the same varieties. 5) The contents of S and Cd of polluted area mulberry leaves was increased about 30% and 300% respectively than non-polluted area. 6) The fed part of non-polluted mulberry leaves was S 0.41% and Cd 0.013 ppm water-cleaned part was S 0.47% and Cd 0.024 ppm and polluted part was S 0.52% and Cd 0.042 ppm in the contents of S and Cd of silkworm larvae. 7) The contents of S and Cd didn't make visible injury in mulberry leaves but made it seriously in silkworm larvae. 8) The injury of dust on mulberry leaves was more serious than that of quality of mulberry leaves by air-pollution in the economic characters of silkworm. 9) As above results, Chinese descent should avoid contaminated area and it rearing by water-cleaned mulberry leaves can get noticable reults in inevitable case on selection of parents silkworm rearing-zone.
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肝組織에서의 Glucagon의 Cholesterol合成 抑制機轉에 對한 硏究
李根培,車鐘希,朴悅,高光三 朝鮮大學校 附設 醫學硏究所 1983 The Medical Journal of Chosun University Vol.8 No.1
The inhibitory mechanism of glucagon on hepatic cholesterol biosynthesis in rat was studied by the measurement of incorporation of tritium from ^(3)H₂O into cholesterol, and intermediary metabolite of lipids. But the rate of cholesterol synthesis was not affected. The hepatic acetyl-CoA carboxylase activity and malonyl-CoA concentration were decreased to 25~35% of control value during the same period after glucagon injection. These data indicates that one site of action of glucagon in regulating fat biosynthesis is at the acetyl-CoA carboxylase reaction.
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Preparation of Monodisperse Colloidal ZnO Nanoparticles and Their Optical Properties
Chong Yang,Limei Tang,Qingsong Li,Ailing Bai,Yanqiu Wang,Yingmin Yu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.5
Monodisperse colloidal zinc oxide (ZnO) nanospheres with a narrow size distribution were synthesized via a developed two-stage solution method. We controlled the size of the as-synthesized ZnO nanoparticles by varying the amount of ZnO/ethanol suspension added. Dynamic light scattering (DLS) and transmission electron microscopy (TEM) revealed that the diameter of the as-synthesized ZnO nanoparticles was in the range of 60 – 140 nm with a polydispersity index less than 5%. On high-resolution TEM images, we clearly observed that the ZnO nanospheres were actually composed of tiny ZnO subunits, several nanometers in size. Powder X-ray diffraction and TEM-selected area electron diffraction analysis showed that the spheres consisted of polycrystalline nanoparticles. The size of the subunits, which was confirmed by ultraviolet (UV)-visible spectroscopy, increased as the amount of ZnO/ethanol suspension added was decreased. A UV emission at about 374 nm was observed, and this emission of ZnO nanoparticles is found to depend on particle size due to the confinement effect. A red emission at about 651 nm, which has been reported for undoped ZnO, appeared due to the excess oxygen on the particles from O – H or C=O groups. The intensity of the red emission increased as the relative oxygen content increased. The formation mechanism of such ZnO nanospheres was also considered.
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發育鷄組織 Glutamic-Oxaloacetic 및 Glutamic-Pyruvic Transaminase에 關한 硏究
黃鍾業,李根培 우석대학교 의과대학 1969 우석의대잡지 Vol.6 No.1
In the Present studies, the activities of glutamic-oxaloacetic (GOT) and glutamic-pyruvic (GPT) transaminases of chick embryo have been studied. Crude enzymes were prepared from whole embryo up to 17 incubation day and liver from 18th day of incubation up to 10 days old chick. Tissues were homogenized in a Teflon-pestle homogenizer with 9 volumes of phosphate buffer (PH 7.4). The crude enzyme (0.1-0.05㎎) was added in reaction mixture. GOT and GPT activities were assayed by colorimetric method of Reitman and Frankel. The results obtained were as follows: 1. GOT activity was found in unincubated egg yolk. The average levels of activity were GOT 88 units/㎎ wet wt.. 2. In the whole embryo GOT increased 4.5 fold from 145 units/㎎ wet wt. at 1st day of incubation to 650 units at 17th day of incubation. GPT increased 2.6 fold during this period from 130 to 340 units/㎎ wet wt.. GOT activity was always greater than GPT activity during development. In the liver of 18 day-old chick embryo GOT and GPT levels of activity were 950 and 620 units/㎎ wet wt. respectively, and there were a slight increases in the activity up to 21th day of incubation. After hatching, GOT and GPT activities showed a marked decrease during 5 days and continued the levels of 500 and 300 units/㎎ wet wt. respectively. 3. The whole embryo possessed GOT 220 units and GPT 180 units per ㎎ or protein at the 1st day of incubation. The sharp increase in both enzyme activities was observed in the embryos from 2 to 6 days, followed by a gradual increase up to the 17th day of incubation examined. GOT increased 5 fold during this period from 220 to 1,000 units per ㎎ of protein. GPT increaed 3 times from 180 to 560 units/㎎ protein. The GOT and GPT activities of 18-days old embryonic liver were 1,600 units/㎎g protein and 1,000 units/㎎ protein respectively, and sowed a sharp rise up to hatching and then dropped markedly during 5 days after the hatching. At 3 to 8 days after hatching the levels of GOT and GPT activities were 1,000 units/㎎ protein and 500 units/㎎ protein respectively. The time course changes in the GOT and GPT activities expressed per ㎎ of wet wt. and expressed per ㎎ of protein during development, showed almost similar fluctuation. 4. Considering the specific aspects of enzyme development the importance of using a proper basis of reference for the expression of enzyme activities in developing tissues was discussed with relation to protein synthesis in embryonic cells.
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