이근배 시인 소장 벼루와 벼루 주제 시

이근배 문예운동사 2021 문예운동 Vol.- No.151

[심사평] 출구를 잃어버린 인간들에게 보내는 ‘담담한 메시지’

이근배,이우걸 고요아침 2023 시조시학 Vol.86 No.-

최소 절개 봉합술을 이용한 아킬레스건 파열의 치료 - 수술 방법 및 초기 결과 -

이근배,박유복,김병수,최진,정성택,Lee, Keun-Bae,Park, Yu-Bok,Kim, Byung-Soo,Choi, Jin,Jung, Sung-Taek 대한족부족관절학회 2006 대한족부족관절학회지 Vol.10 No.1

Purpose: To investigate the early results of limited open repair technique of Achilles tendon ruptures, and to describe the surgical technique. Materials and Methods: From October 2004 to February 2005, a total of 10 patients with Achilles tendon rupture underwent limited open repair. The average age of the patients was 39.3 years, and the average follow-up period was 9 months. The causes of injury were sports injuries in 8 cases, and slip down in 2. The mean interval between the injury and the operation was 9 days. The clinical results were assessed by patient's satisfaction, incision length, hospitalization, the ankle-hindfoot scale of American Foot and Ankle Society (AOFAS), and complications. Results: Of 10 patients, 8 were very satisfied, and the remaining 2 were satisfied. The mean incision length was 2.0 cm, and the mean hospitalization was 2 days. The mean AOFAS score was 97 points, and there was no complications such as infection, rerupture, or nerve injury. All patients returned to work at approximately 2 months, and resumed light exercise such as jogging at approximately 3 months. Conclusion: Limited open repair technique of Achilles tendon ruptures is provided for better cosmetic results, high patient's satisfaction, and functionally successful results without postoperative complications.

마우스 간세포의 핵 및 핵소체에서의 NAD Synthetase 의 검출

이근배,박인원,조태묵,황운기 한국생화학회 1968 BMB Reports Vol.1 No.2

The activities of NAD pyrophosphorylase, deamido-NAD pryophasphorylase are detected in the organelles of mouse liver cell: nuclei and nucleoli. The three enzymes were partially purified by protamine sulfate. All three enzymes are distributed both in nuclei and nucleoli. The test of the localization of the enzyme activities shows that nucleus, as a whole, is the more active site of NAD synthesis than nucleolus is. It appears, however, that the enzymes of nucleus and nucleolus are localized per se independently in each organelle.

Glycol 산 산화효소의 정제 및 성상

이근배,채영복,박성희 ( Keun Bai Lee,Young Bok Chae,Sung He Park ) 생화학분자생물학회 1969 BMB Reports Vol.2 No.2

A 680-fold purifcation of glycolate oxidase (glycolate : 02 oxidoreductase. EC 1. 1. 3. 1) has been achieved from 20,000 × g supernatant fraction of germinating Soy bean seed embryos by means of ammonium sulfate precipitation. acid precipitation, Sephadex G-100 gel filtration and DEAE-cellulose column chromatography. The pH optimum for the reaction is 8.0 and apparent Michaelis constant was estimated as 1.0 × 10^(-4) M for glycolic acid. Disc electrophoresis on acrylamide gel was carried out at various purification steps of the enzyme, and the final preparation was found to consist of one protein component. The molecular weight of the enzyme was estimated to be approximately 68,000 by Sephadex G-100 gel filtration. FMN and FAD were found to be efficient cofactors, FMN being more favorable than FAD. The enzyme was activated by KCN and inhibited by p-chloromercuribenzoate and iodoacetate,

Purification and Properties of Two Glyoxylate Reductases from Germinating Soybean Seed Embryos

이근배,이희성,이강석,Lee, Keun-Bai,Lee, Hi-Sung,Lee, Kang-Suk 생화학분자생물학회 1970 한국생화학회지 Vol.3 No.2

발아중인 대두의 배에서 두 종류의 glyoxylate reductase(glycoIIate-NAD oxidoreductase, EC. 1. 1. 1. 26)를 추출하고 ammonium sulfate에 의한 침전, Sephadex G-200 gel filtration, DEAE-cellulose 및 Sephadex G-100 column chromatography에 의하여 정제하였다. 두 종류의 효소는 각각 NADH 및 NADPH를 cofactor로 요구하는 바 각각 745배 및 789배 정제하였다. 최종적으로 분리 정제된 상기 효소는 sucrose density gradient centrifugation, starch 및 acrylamide gel electrophoresis를 행하여 단일 효소 단백임을 확인하였다. NADH-linked glyoxylate reductase의 Michaelis constant, Km은 $9.0{\times}10^{-3}M$ 이다. 또한 NADH-Iinked enzyme 및 NADPH-linked enzyme의 분자량은 Sephadex G-100 gel filtration으로 측정하여 각각 142,000 및 192,000 이었다. 이 효소의 최적 pH, 최적온도, thiol group 저해제 및 몇 가지의 금속 ion이 효소활성에 미치는 영향을 검토하였다. Two protein fractions which catalyse the reduction of glyoxylate to glycollate were isolated from germinating soybean seed embryos by ammonium sulfate precipitation, Sephadex G-200 gel filtration. DEAE-cellulose column chromatography and rechromatography on Sephadex G-100. One protein fraction required NADH and another protein fraction required NADPH as cofactor. These two enzymes were purified 745-fold and 789-fold, respectively. The final preparations were homogeneous by sucrose density gradient centrifugation, starch and acrylamide gel electrophoresis. The apparent Michaelis constant. Km, of NADH-linked glyoxylate reductase was estimated as approximately $9.0{\times}10^{-3}M$. Molecular weights of NADH-Iinked and NADPH-linked enzymes were estimated to be about 142,000 and 192,000, respectively, by Sephadex G-100 gel filtration. Effects of pH, temperature, thiol group inhibitors and various metal ions on the enzyme were also described.

종양조직의 Transfer Ribonucleic Acid 의 Methyl 화에 관한 연구

이근배,이희성,이강석,김기수 ( Keun Bai Lee,Hi Sung Lee,Kang Suk Lee ) 생화학분자생물학회 1971 BMB Reports Vol.4 No.1

Studies on transfer ribonucleic acid (tRNA) methylases of normal tissues, Walker 256 carcinosarcoma and Sarcoma 180 ascites tumor cells have been carried out with respect to: 1) rate of methylation of tRNA from rabbit liver, Walker 256 carcinosarcoma, Sarcoma 180 ascites tumor cells and E. coli B and 2) an attempt was also made to search out the pattern of methylation, i.e., the specific bases methylated by the extracts of tumor tissues. The homogenate was centrifuged at 100,000 xg for 10 min and the supernatant fluid was used as the enzyme extracts. In vitro methylation of tRNA was carried out by incubating enzyme extract with substrate (tRNA) and ATP generating system. ^(14)C-methyl-L-methionine was used as methyl donor. Resulting methylated tRNA was hydrolyzed and chromatographed on Whatman paper. Spots on the chromatograms were identified by measuring the Rf and UV absorption spectra and cut into strips and counted directly in a Beckman LS-100 liquid scintillation counter. The results obtained were as follows 1. The tRNA methylases of tumor tissues exhibited the elevaton of methylating potencies about three-fold increase when methylated homologously. 2. The methylase activity of tumor tissues was increased 4-5 times over that of normal controls. The enzyme extracted from Walker 256 carcinosarcoma served as the most appropriate source. 3. Several lines of evidence for the binary nature of the tRNA methylase from tumor tissues were demonstrated. Results of heterologous methylations with a variety of substrate reveal that extracts of tumor tissues introduce more methyl group into adenine residue than into guanine residue of tRNA. 4. Exposure of tRNA to normal methylases followed by methylases from tumor tissues has indicated that the tumor tRNA methylases introduced methyl groups into different positions on the tRNA molecule than do normal enzymes. Among hydrolysate of methylated tRNA, a novel methylated pyrimidine, i.e., N,N`-dimethylcytosine and an unidentified base were detectable. The only extract from Walker 256 carcinosarcoma was found to possess methylase activity for the synthesis of N,N`-dimethylcytosine.

방사선 조사가 Sarcoma 180 Ascites Tumor Cells 의 Histone 생합성에 미치는 영향

이근배,이희성 ( Keun Bai Lee,Hi Sung Lee ) 생화학분자생물학회 1972 BMB Reports Vol.5 No.2

In the earlier works from this laboratory, biosynthesis, acetylation, methylation and phosphorylation of histone of transplantable ascites tumor cells have been studied. The present paper deals with the effects of X-ray irradiation on histone biosynthesis of Sarcoma 180 ascites tumor cells. The tumor-bearing mice were irradiated with a dose of 1250 roentgen prior to 24 hours double-labeling with 10 μCi of glycine-l-^(14)C and 20 μCi of ^(32)P-orthophosphate. Sham irradiated mice were used as control. Histones were prepared by Johns procedure and fractionated on polyacrylamide disc gel electrophoresis. The histones were hydrolyzed in acid and the digests were chromatographed on an amino acid analyzer. DNA was extracted according to the method of Ogur and Rosen. Radioactivity was counted in a liquid scintillation spectrometer. The time course changes in the incorporations of labeled compounds into histone and DNA have been followed. Results obtained were as follows ; 1. Contents of histone fractions from Sarcoma 180 ascites tumor cells have been shown to markedly differ from normal tissues, i.e. mouse liver, rat and calf thymus. 2. X-ray irradiation hindered ^(14)C-glycine incorporation into histone by 14 per cent. Uptake of ^(14)C was highest into f3 and was lowest into f2b among histone fractiones both in normal and irradiated mice. The incorporation of ^(14)C-glycine into histone reached a maximum 4 hours after glycine administration. 3. Radioactivity of ^(14)C-glycine was less distributed about 12 per cent in glycine fraction of histone hydrolysates from X-ray irradiated animal. 4. A decreased ^(32)P-incorporation (about 14%) into DNA was observed in irradiated mice. Relationships between histone biosynthesis and DNA metabolism were also discussed.

Histone 에 관한 연구 ( Ⅸ ) Walker 256 carcinosarcoma 의 Histone 에 대하여

이근배,이희성,라석찬 ( Keun Bai Lee,Hi Sung Lee,Suck Chan Rha ) 생화학분자생물학회 1977 BMB Reports Vol.10 No.3

In attempt to investigate histone fractions of tumor cells, nuclei was isolated from Walker 256 carcinosarcoma by the procedure of Pogo et al., Histone fractions Hl, H2a, H2b, H3 and H4 were prepared from isolated nuclei by the procedure of Johns. The five histone fractions found in most tissues were also present in the Walker 256 carcinosarc:oma histones. These histone fractions were characterized by amino acid analysis and by polyacrylamide disc gel electrophoresis. The results obtained were as follows: 1. The yield of whole histone was 3.75me per g of Walker 256 carcinosarcoma. 2. The yield of DNA was 3.35mg per g of Walker 256 carcinosarcorna. Consequently the DNA to histone ratio was 1 : 1.12. 3. The relative amounts of five fractions, i.e., Hl, H2a, H2b, H3 and H4 were 20.00%, 9.33 %, 26.67%, 33.33 % and 10.67%, respectively. 4. The electrophoretic mobility of individual histone fractions gave almost similar patterns to those of corresponding fractions of calf thymus. 5. Amino acid analysis of the individual histone fractions showed that the over-all com positions were similar but not identical to those of the corresponding fraction from calf thymus. 6. We found that histone H2b fraction of Walker 256 carcinosarcoma contained detectable amounts of ε-N-monomethyllysine and ε-N-dimethyllysine. No evidence for the presence of methylated lysine or other side-chain derivatives was reported on this histone fraction. We also found that histcne H1 fraction contained detectable amounts of 3-methylhistidine.

[심사기] 사설을 풀어내는 이달균의 입담

이근배 고요아침 2019 시조시학 Vol.70 No.-