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發育鷄組織 Glutamic-Oxaloacetic 및 Glutamic-Pyruvic Transaminase에 關한 硏究
黃鍾業,李根培 우석대학교 의과대학 1969 우석의대잡지 Vol.6 No.1
In the Present studies, the activities of glutamic-oxaloacetic (GOT) and glutamic-pyruvic (GPT) transaminases of chick embryo have been studied. Crude enzymes were prepared from whole embryo up to 17 incubation day and liver from 18th day of incubation up to 10 days old chick. Tissues were homogenized in a Teflon-pestle homogenizer with 9 volumes of phosphate buffer (PH 7.4). The crude enzyme (0.1-0.05㎎) was added in reaction mixture. GOT and GPT activities were assayed by colorimetric method of Reitman and Frankel. The results obtained were as follows: 1. GOT activity was found in unincubated egg yolk. The average levels of activity were GOT 88 units/㎎ wet wt.. 2. In the whole embryo GOT increased 4.5 fold from 145 units/㎎ wet wt. at 1st day of incubation to 650 units at 17th day of incubation. GPT increased 2.6 fold during this period from 130 to 340 units/㎎ wet wt.. GOT activity was always greater than GPT activity during development. In the liver of 18 day-old chick embryo GOT and GPT levels of activity were 950 and 620 units/㎎ wet wt. respectively, and there were a slight increases in the activity up to 21th day of incubation. After hatching, GOT and GPT activities showed a marked decrease during 5 days and continued the levels of 500 and 300 units/㎎ wet wt. respectively. 3. The whole embryo possessed GOT 220 units and GPT 180 units per ㎎ or protein at the 1st day of incubation. The sharp increase in both enzyme activities was observed in the embryos from 2 to 6 days, followed by a gradual increase up to the 17th day of incubation examined. GOT increased 5 fold during this period from 220 to 1,000 units per ㎎ of protein. GPT increaed 3 times from 180 to 560 units/㎎ protein. The GOT and GPT activities of 18-days old embryonic liver were 1,600 units/㎎g protein and 1,000 units/㎎ protein respectively, and sowed a sharp rise up to hatching and then dropped markedly during 5 days after the hatching. At 3 to 8 days after hatching the levels of GOT and GPT activities were 1,000 units/㎎ protein and 500 units/㎎ protein respectively. The time course changes in the GOT and GPT activities expressed per ㎎ of wet wt. and expressed per ㎎ of protein during development, showed almost similar fluctuation. 4. Considering the specific aspects of enzyme development the importance of using a proper basis of reference for the expression of enzyme activities in developing tissues was discussed with relation to protein synthesis in embryonic cells.
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