Isolation and Characterization of Water Soluble Bioactive Substances from Marine Photosynthetic Microalga Tetraselmis Species

( Babita Dogra ),( Da Gyung Lee ),( Jae Kweon Park ) 한국키틴키토산학회 2017 한국키틴키토산학회지 Vol.22 No.1

Isolation, quantification and characterization of bioactive compounds from microalgae Tetraselmis species have been performed to elucidate the potential use as a bioactive material. Microalga are a promising source of lipids for biofuel production and represent a diverse group of microscopic photosynthetic organisms and are able to convert solar energy into biomasses. The imposition of oxidative stresses by various environmental factors leads to the production of reactive oxygen species (ROS) in plant cells, including algal cell. Antioxidant activity of water soluble bioactive substances (WSBS) was determined by DPPH, ABTS, FRAP and similar SOD radical-scavenging assay. The content analysis of lipid, sugar, polyphenol and protein was performed to evaluate the potent usefulness of WSBS from the whole cells cultivated under the optimum conditions for the production of biodiesel. The yield of WSBS was determined to be 17% based on the total carbohydrate assay. The relative activity of WSBS toward FRAP and DPPH reagent was observed ranging from 13 to 55%, showing the significant anti-oxidative properties with wide range of temperatures from room temperature to 80℃. The outcomes of this study support utilization of WSBS from microalgae Tetraselmis species as a potential source of naturally occurring antioxidants.

Comparison of ultrasound guided pulsed radiofrequency of genicular nerve with local anesthetic and steroid block for management of osteoarthritis knee pain

( Babita Ghai ),( Muthu Kumar ),( Jeetinder Kaur Makkar ),( Vijay Goni ) 대한통증학회 2022 The Korean Journal of Pain Vol.35 No.2

Background: To compare ultrasound-guided pulsed radiofrequency (PRF) of the genicular nerve with the genicular nerve block using local anesthetic and steroid for management of osteoarthritis (OA) knee pain. Methods: Thirty patients with OA knee were randomly allocated to receive either ultrasound-guided PRF of the genicular nerve (PRF group) or nerve block with bupivacaine and methylprednisolone acetate (local anesthetic steroid [LAS] group). Verbal numeric rating scale (VNRS) and Western Ontario McMaster Universities Osteoarthritis Index (WOMAC) scores were measured at pre-procedure and 1-, 4-, and 12-weeks post-procedure. Results: VNRS scores decreased significantly (P < 0.001) in both the groups at 12 weeks and other follow up times compared to baseline. Seventy-three percent of patients in the PRF group and 66% in the LAS group achieved effective pain relief (≥ 50% pain reduction) at 12 weeks (P > 0.999). There was also a statistically significant (P < 0.001) improvement in WOMAC scores in both groups at all follow up times. However, there was no intergroup difference in VNRS (P = 0.893) and WOMAC scores (P = 0.983). No complications were reported. Conclusions: Both ultrasound-guided PRF of the genicular nerve and blocks of genicular nerve with local anesthetic and a steroid provided comparable pain relief without any complications. However, PRF of the genicular nerve is a procedure that takes much more time and equipment than the genicular nerve block.

Biochemical Properties of Water Soluble Polysaccharides from Photosynthetic Marine Microalgae Tetraselmis Species

Babita Dogra,Shaheen Amna,박용일,박제권 한국고분자학회 2017 Macromolecular Research Vol.25 No.2

Water soluble polysaccharides (WSPs) were isolated from defatted microalgae Tetraselmis sp. KCTC 12432 BP (T.S1) and KCTC 12236 BP (T.S2), and purified by a combination of ethanol precipitation, immobilized metal affinity chromatography and size-exclusion column chromatography. Biochemical properties of WSPs obtained from the supernatant (S 1 and S 2) and purification steps (EP 1 and EP 2) have been elucidated for their potential use as a biologically active material. Oxidative effect on efficiency from the hydrolytic fenton reaction (FS 1 and FS 2) was also employed to assess the WSPs production and biological activity from T.S1 and T.S2, respectively. Antioxidant activity of the purified WSPs from EP 2 toward 1,1-diphenyl-2-picryl-hy-drazyl (DPPH), 2,2’-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid) di-ammonium salt (ABTS), and ferric reducing antioxidant power (FRAP) of radical-scavenging showed significant differences depending on the monosaccharide compositions of WSPs. On the other hand, a significant specific tyrosinase inhibitory activity was observed from the WSPs purified from EP 1 (10 μmol/g/min) indicating the potential use as a whitening source. The outcomes of our study support the utilization of WSPs from these microalgal species as a potential source of naturally occurring whitening and antioxidants to ease oxidative stresses. According to our knowledge related to microalgal studies, until now this is the first study which demonstrates the significance between primary structure and potential biological activities of WSPs purified from the defatted microalgae Tetraselmis species.

Extended duration pulsed radiofrequency for the management of refractory meralgia paresthetica: a series of five cases

( Babita Ghai ),( Deepanshu Dhiman ),( Sekar Loganathan ) 대한통증학회 2018 The Korean Journal of Pain Vol.31 No.3

Meralgia paresthetica (MP) is a sensory mononeuropathy, caused by compression of the lateral femoral cutaneous nerve (LFCN) of thigh. Patients refractory to conservative management are treated with various interventional procedures. We report the first use of extended duration (8 minutes) pulsed radiofrequency of the LFCN in a case series of five patients with refractory MP. Four patients had follow up for 1-2 years, and one had 6 months follow up. All patients reported remarkable and long lasting symptom relief and an increase in daily life activities. Three patients came off medications and two patients required minimal doses of neuropathic medications. No complications were observed. (Korean J Pain 2018; 31: 215-20)

Antioxidant Activity of Chitosan Hydrolysates Derived by Microwave-assisted Irradiation

Babita Kumari,남근영,김정민,이용현,황유진,박제권 한국키틴키토산학회 2017 한국키틴키토산학회지 Vol.22 No.4

Degradation of chitosan by free radical cleavage at 90 oC in the presence of ammonium persulfate was performed. Typically, high molecular weight chitosan was dissolved in 2% acetic acid and then submitted to the Microwave-assisted and room temperature using different time. The in vitro quantification analysis and antioxidant activity of self-assembled and hydrolyzed chitosan was evaluated and compared as radical scavengers against 1,1- diphenyl-2-picrylhydrazyl radicals (DPPH•), 2,2’- Azinobis (3-ethylbenzothiazoline-6-sulfonic acid) di-ammonium salt (ABTS), and ferric reducing antioxidant power (FRAP) using established methods and the effect of microwave and room temperature on the antioxidant activity of these molecular weight chitosan is discussed. Our data suggested the different type chitosan hydrolysates could be effectively employed as natural antioxidant materials for the various applications such as cosmeceutical and other potent industry products.

Type 2 Fuzzy Stochastic Transportation Problem with Gamma Distribution

Babita Chaini,Narmada Ranarahu 한국지능시스템학회 2023 INTERNATIONAL JOURNAL of FUZZY LOGIC and INTELLIGE Vol.23 No.1

The transportation problem in real life is an uncertain problem. Particularlywhen goods are transported from the source to destinations with the best trans-portation setup that satis es the decision maker's preferences by taking into ac-count the competing objectives/criteria such as maintaining exact relationshipsbetween a few linear parameters, such as actual transportation fee/total transporta-tion cost, delivery fee/desired path, total return/total investment, etc. Due to the uncertainty of nature, such a relationship is not deterministic. In this stochastic transportation problem supplies are considered as fuzzy random variables, which follow fuzzy gamma distribution, with shape parameter and scale parameter. Here is a perfectly normal interval type-2 fuzzy random variable. This paper proposes a solution methodology for solving the fuzzy stochastic transportation problem, where fuzziness and randomness occur under one roof. Therefore, we converted it to an equivalent deterministic mathematical programming problem by applying the following two steps. In the first step of the solution procedure, fuzziness is removed by using alpha-cut technique to obtain stochastic transportation problem. In the second step, the stochastic transportation problem is converted to an equivalent crisp transportation problem using the chance constrained technique. This mathematical model is solved by existing methodology or software. In order to illustrate the methodology a case study is provided.

Gelatin coated magnetic iron oxide nanoparticles

Gaihre Babita,길명섭,강효경,김학용 한국고분자학회 2008 한국고분자학회 학술대회 연구논문 초록집 Vol.33 No.1

Antibacterial potential of Antarctic lichens against human pathogenic Gram‐positive bacteria

Paudel, Babita,Bhattarai, Hari Datta,Lee, Jin Sung,Hong, Soon Gyu,Shin, Hyun Woung,Yim, Joung Han John Wiley Sons, Ltd. 2008 Phytotherapy research Vol.22 No.9

<P><B>Abstract</B></P><P>Extracts from five Antarctic lichens (L3, <I>Stereocaulon alpinum</I>; L5, <I>Ramalina terebrata</I>; L6, <I>Caloplaca</I> sp.; L8, <I>Lecanora</I> sp.; and L17, <I>Caloplaca regalis</I>) were tested for antimicrobial activities against several clinically important microbes by disk diffusion. The minimum inhibitory concentration (MIC) of each extract was determined by a broth dilution method. Extracts from L3, L5, L6 and L8 were active against two Gram(+) strains. <I>B. subtilis</I> was more sensitive to lichen extracts (except L5) than <I>S. aureus</I>. The MIC of lichen extracts against <I>B. subtilis</I> and <I>S. aureus</I> was observed from 36.7 ± 0.3 to 953.8 ± 85.8 µg/mL and 68.5 ± 0.6 to >1000 µg/mL, respectively. Comparisons of MIC values of Antarctic lichen crude extracts to previously published MIC values of some reported lichen metabolites against Gram(+) bacteria indicated that Antarctic lichens might be an enriched source of effective antibacterial agents against clinically relevant Gram(+) species. Copyright © 2008 John Wiley & Sons, Ltd.</P>

Estimation of antioxidant, antimicrobial activity and brine shrimp toxicity of plants collected from Oymyakon region of the Republic of Sakha (Yakutia), Russia

Paudel, Babita,Bhattarai, Hari Datta,Kim, Il Chan,Lee, Hyoungseok,Sofronov, Roman,Ivanova, Lena,Poryadina, Lena,Yim, Joung Han BioMed Central 2014 BIOLOGICAL RESEARCH Vol.47 No.1

<P><B>Background</B></P><P>Several plants are reported to be produced various biological active compounds. Lichens from the extreme environments such as high altitude, high UV, drought and cold are believed to be synthesized unique types of secondary metabolites than the other one. Several human pathogenic bacteria and fungi have been muted into drug resistant strains. Various synthetic antioxidant compounds have posed carcinogenic effects. This phenomenon needs further research for new effective drugs of natural origin. This manuscript aimed to screen new source of biological active compounds from plants of subarctic origin.</P><P><B>Results</B></P><P>A total of 114 plant species, including 80 species of higher plants, 19 species of lichens and 15 species of mosses, were collected from Oymyakon region of the Republic of Sakha (Yakutia), Russia (63˚20′N, 141˚42′E–63˚15′N, 142˚27′E). Antimicrobial, DPPH free radical scavenging and brine shrimp (Artemia salina) toxicity of all crude extract were evaluated. The obtained result was analyzed and compared with commercial standards. A total of 28 species of higher plants showed very strong antioxidant activity (DPPH IC50, 0.45-5.0 μg/mL), 13 species showed strong activity (DPPH IC50, 5-10 μg/mL), 22 species showed moderate antioxidant activity (DPPH IC50,10-20 μg/mL) and 17 species showed weak antioxidant activity (DPPH IC50 more than 20 μg/mL). Similarly, 3 species of lichen showed strong antioxidant activity, one species showed moderate and 15 species showed weak DPPH reducing activity. In addition, 4 species of mosses showed moderate antioxidant activity and 11 species showed weak antioxidant activity. Similarly, extracts of 51 species of higher plants showed antimicrobial (AM) activity against Staphylococcus aureus and 2 species showed AM activity against Candida albicans. Similarly, 11 species of lichen showed AM activity against S. aureus and 3 species showed AM activity against Escherichia coli. One species of moss showed AM activity against S. aureus. And finally, one species of higher plant Rheum compactum and one species of lichen Flavocetraria cucullata showed the toxicity against Brine shrimp larvae in 100 μg/mL of concentration.</P><P><B>Conclusion</B></P><P>The experimental results showed that subarctic plant species could be potential sources of various biologically active natural compounds.</P>

Biomass derived activated carbon loaded silver nanoparticles: An effective nanocomposites for enhanced solar photocatalysis and antimicrobial activities

Th. Babita Devi,Dipyaman Mohanta,Md. Ahmaruzzaman 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.76 No.-

This study discusses the prospect of using a biomass waste material, such as jute stick in the developmentof new technological material for the sustainable environmental application. In this article, wesynthesized silver loaded activated carbon (Ag-AC) nanocomposite using a chemical precipitationmethod. The as-synthesized Ag-AC nanocomposite exhibited excellent antimicrobial activity towardgram negative and positive bacteria, such as Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Bacillus subtilis (B. subtilis) and Staphylococcus aureus (S. aureus). This may be because of thefact that the antibacterial effect of the silver nanoparticles was enhanced due to the presence of activatedcarbon (AC). We also compared the antimicrobial activities of Ag-AC nanocomposite with metallicnanoparticles (silver) and it was observed that the nanocomposite showed better antimicrobial propertythan that of metallic silver nanoparticles. The Ag-AC nanocomposite may, therefore, have the prospectsfor use as an antibacterial agent. In the present study, we also investigated the catalytic activity of Ag-ACnanocomposite for the degradation of toxic dye (malachite green oxalate) and pharmaceutical activecompound (Clofibric acid) under solar irradiation. The malachite green oxalate and Clofibric acid werecompletely degraded within 90 min and 80 min using Ag-AC nanocomposite. Hence, the Ag-ACnanocomposite showed good antimicrobial as well as an excellent photocatalytic activity.