국내 분리 일본뇌염 바이러스 유전자에 대한 항체생성 연구(Ⅰ) : cloning of JEV viral genes

박찬,최우영,이호동,채수림,신은진,김인범,표재환,박근용,조해월 국립보건연구원 1999 국립보건원보 Vol.36 No.-

Photoluminescence and white light generation behavior of lithium gadolinium silicoborate glasses

Shamshad, L.,Rooh, G.,Kirdsiri, K.,Srisittipokakun, N.,Damdee, B.,Kim, H.J.,Kaewkhao, J. Elsevier 2017 JOURNAL OF ALLOYS AND COMPOUNDS Vol.695 No.-

<P><B>Abstract</B></P> <P>The Dy<SUP>3+</SUP>-doped lithium gadolinium silica borate glasses with composition (in mol%) 40Li<SUB>2</SUB>O:15Gd<SUB>2</SUB>O<SUB>3</SUB>:5SiO<SUB>2</SUB>: (40-x)B<SUB>2</SUB>O<SUB>3</SUB>:xDy<SUB>2</SUB>O<SUB>3</SUB>, x = 0.01, 0.1, 0.5, 1.0, 2.0 were prepared by melt quenching technique and characterized through absorption and emission spectra, CIE chromaticity coordinates and decay rate analysis. Judd-Oflet parameters have been calculated for lithium gadolinium silico borate glass and used for the derivation of radiative properties for excited luminescent levels of Dy<SUP>3+</SUP> ions. The feasibility of white light generation have been evaluated by the yellow-to-blue emission intensity ratios and CIE chromaticity coordinates as a function of Dy<SUP>3+</SUP> concentration. The non-exponential decay rates are well-fitted to Inokuti-Hirayama model for S = 6. The perceived non-exponential decay nature and life time quenching have been ascribed to the energy transfer between excited and unexcited Dy<SUP>3+</SUP> ions through dipole–dipole interaction. The CCT values for the present studied glasses obtained in the 4584-4235 K at λ<SUB>exc</SUB> = 387 nm. These CCT values are below the warm CCT (i.e CCT 4000 K). The reasonably good luminescence properties of LGSiBDy10 glass indicates the potentiality of the title of glass to be applied as lasing medium for white light generation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Spectroscopic properties of Dy<SUP>3+</SUP> ion in Li<SUB>2</SUB>OGd<SUB>2</SUB>O<SUB>3</SUB> SiO<SUB>2</SUB> B<SUB>2</SUB>O<SUB>3</SUB> were studied. </LI> <LI> JO analysis were used to analyze spectroscopic properties. </LI> <LI> White emission was observed from glass sample. </LI> <LI> Lasing properties were characterized. </LI> </UL> </P>

Polymorphisms of <i>KCNJ11</i> (Kir6.2 gene) are associated with Type 2 diabetes and hypertension in the Korean population

Koo, B. K.,Cho, Y. M.,Park, B. L.,Cheong, H. S.,Shin, H. D.,Jang, H. C.,Kim, S. Y.,Lee, H. K.,Park, K. S. Blackwell Publishing Ltd 2007 Diabetic medicine Vol.24 No.2

<P>Abstract</P><P>Aims </P><P>Kir6.2 is found in the pancreatic B-cell, cardiac and skeletal muscle and non-vascular smooth muscle. <I>KCNJ11</I>, encoding Kir6.2, has been shown to be associated with both Type 2 diabetes mellitus and cardiovascular disease in several populations. In this study, we investigated whether polymorphisms in <I>KCNJ11</I> are associated with Type 2 diabetes and other metabolic phenotypes in the Korean population.</P><P><B>Methods </B></P><P>We sequenced <I>KCNJ11</I> to identify common polymorphisms using 24 Korean DNA samples. Of the 14 polymorphisms found in <I>KCNJ11</I>, six common ones [genomic sequence (g.)−1709A>T, g.−1525T>C, g.67G>A (E23K), g.570C>T (A190A), g.1009A>G (I337V), and g.1388C>T] were genotyped in 761 Type 2 diabetic patients and in 630 non-diabetic subjects.</P><P><B>Results</B> </P><P>All the polymorphic loci in <I>KCNJ11</I> are in strong linkage disequilibrium in the Korean population and act as one haplotype block. g.67G>A and g.1009A>G were associated with an increased risk of Type 2 diabetes [age, sex, and body mass index (BMI)-adjusted odds ratios (OR) = 1.376 (1.085–1.745), <I>P</I> = 0.008 and 1.411 (1.111–1.791), <I>P</I> = 0.005, respectively], as was one haplotype (A-T-A-C-G-C in the order of polymorphisms as shown above) containing g.67A and g.1009G [OR = 1.359 (1.080–1.709), <I>P</I> = 0.009]. The haplotype (A-T-A-C-G-C) was also strongly associated with hypertension [OR = 1.655 (1.288–2.126), <I>P</I> < 0.001].</P><P><B>Conclusions </B></P><P>Polymorphisms in <I>KCNJ11</I> are associated with Type 2 diabetes and also with hypertension in the Korean population.</P>

Effects of L-tryptophan, Fructan, and Casein on Reducing Ammonia, Hydrogen Sulfide, and Skatole in Fermented Swine Manure

Q.K. Sheng,Z.J. Yang,H.B. Zhao,X.L. Wang,J.F. Guo 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.8

The effects of daily dietary Bacillus subtilis (Bs), and adding L-tryptophan, fructan, or casein to fecal fermentation broths were investigated as means to reduce the production of noxious gas during manure fermentation caused by ammonia, hydrogen sulfide (H2S), and 3-methylindole (skatole). Eighty swine (50.0±0.5 kg) were equally apportioned to an experimental group given Bs in daily feed, or a control group without Bs. After 6 weeks, fresh manure was collected from both groups for fermentation studies using a 3×3 orthogonal array, in which tryptophan, casein, and fructan were added at various concentrations. After fermentation, the ammonia, H2S, L-tryptophan, skatole, and microflora were measured. In both groups, L-tryptophan was the principle additive increasing skatole production, with significant correlation (r = 0.9992). L-tryptophan had no effect on the production of ammonia, H2S, or skatole in animals fed Bs. In both groups, fructan was the principle additive that reduced H2S production (r = 0.9981). Fructan and Bs significantly interacted in H2S production (p = 0.014). Casein was the principle additive affecting the concentration of ammonia, only in the control group. Casein and Bs significantly interacted in ammonia production (p = 0.039). The predominant bacteria were Bacillus spp. CWBI B1434 (26%) in the control group, and Streptococcus alactolyticus AF201899 (36%) in the experimental group. In summary, daily dietary Bs reduced ammonia production during fecal fermentation. Lessening L-tryptophan and increasing fructan in the fermentation broth reduced skatole and H2S.

Effects of L-tryptophan, Fructan, and Casein on Reducing Ammonia, Hydrogen Sulfide, and Skatole in Fermented Swine Manure

Sheng, Q.K.,Yang, Z.J.,Zhao, H.B.,Wang, X.L.,Guo, J.F. Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.8

The effects of daily dietary Bacillus subtilis (Bs), and adding L-tryptophan, fructan, or casein to fecal fermentation broths were investigated as means to reduce the production of noxious gas during manure fermentation caused by ammonia, hydrogen sulfide ($H_2S$), and 3-methylindole (skatole). Eighty swine ($50.0{\pm}0.5kg$) were equally apportioned to an experimental group given Bs in daily feed, or a control group without Bs. After 6 weeks, fresh manure was collected from both groups for fermentation studies using a $3{\times}3$ orthogonal array, in which tryptophan, casein, and fructan were added at various concentrations. After fermentation, the ammonia, $H_2S$, L-tryptophan, skatole, and microflora were measured. In both groups, L-tryptophan was the principle additive increasing skatole production, with significant correlation (r = 0.9992). L-tryptophan had no effect on the production of ammonia, $H_2S$, or skatole in animals fed Bs. In both groups, fructan was the principle additive that reduced $H_2S$ production (r = 0.9981). Fructan and Bs significantly interacted in $H_2S$ production (p = 0.014). Casein was the principle additive affecting the concentration of ammonia, only in the control group. Casein and Bs significantly interacted in ammonia production (p = 0.039). The predominant bacteria were Bacillus spp. CWBI B1434 (26%) in the control group, and Streptococcus alactolyticus AF201899 (36%) in the experimental group. In summary, daily dietary Bs reduced ammonia production during fecal fermentation. Lessening L-tryptophan and increasing fructan in the fermentation broth reduced skatole and $H_2S$.

만성 임파성 백혈병 1 예 보고와 문헌 고찰

박승광 ( S. K. Park ),유수웅 ( S. W. You ),이봉재 ( B. J. Lee ),성락훈 ( L. H. Sung ) 대한내과학회 1972 대한내과학회지 Vol.15 No.2

The purpose of this report is to illustrate the long course and rarity of C.L.L. in Korea. This is the first case of C.L.L. among 107 all leukemia during the past 10 years in National Medical Center. Variation in the incidence of C.L.L. are compared to oth

江原道 地域에서의 常綠地被植物의 造景的 利用에 關한 基礎的 硏究(Ⅰ) : 增殖, 耐陰性, 耐寒性, 生育量의 差異에 關하여 Progagation, Shade toolerance, Cold resistance, Difference of Growing degree

洪鍾雲,李基哲,許範亮,元慶烈,林炳春 춘천교대 지역개발연구 1994 地域開發硏究 Vol.3 No.-

Experiments were conducted to investigate propagation, shade tolerance, cold resistance and of growth of evergreen ground cover plants; Vinca minor L., Ajuga reptans L., Ophiopogon japonicus ker. f. nanus hort, and Hedera helix. The results were as follows : 1. It is convenient to use the growing case for rice seed to grow and Transplant Vinca minor seeding. The most proper density of Transplanting vinca was 180 plants per 1㎡. At the end of growing season, the coverage came up to 90%. The rooting ability of Hedera helix was best to cut from April to May (temperature 15∼23℃). The seeding length of Parthenocissus quinguefolia were irregular ranged from 5㎝ to 200㎝. 2. Ajuga reptans L., Ophiopogon J. and Vinca minor L. grew better under 50% light intensity than full sunlight. Particularly, under full sunlight and aestival high temperature, there were a few withering plants in Ajuga reptans L. and Vinca minor L. 3. Of Vinca minor L., Ajuga reptans L., Ophiopogon japonicus ker. f. nanus hort. and Hedera helix. acclimatized in open field, where temperature was from -7∼8℃. There were few plants had damage in low temperature incubator of -16℃ after field acclimatization. 4. Tendrils of Parthenocissus quinguefolia strongly clung to the wire netting stone fence but intruded into the gap and cranny of the precast concrete fence and so clung to, so that it needed to install the bending net on the precast concrete fence.

한국 연안에서 Mussel(Mytilus edulis)의 중금속의 생물 농축

이인숙,송준임,박경숙,최병래,노분조 이화여자대학교 생명과학연구소 1995 생명과학연구논문집 Vol.6 No.-

본 연구는 남한 연안의 오염도를 파악하기 위해 마산만, 온산만, 대산공업 단지 및 비오염구에서 해수, 퇴적물 및 진주담치의 구리, 납, 아연 및 카드뮴의 농도를 조사하였다. 해수의 용존성 구리, 납, 아연 및 카드뮴의 농두는 각각 0.29~0.79 ㎍/l, 0.03~0.08 ㎍/l, 0.19~2.01 ㎍/l, 0.01~0.05 ㎍/l 범위를 나타냈으며, 납(p<0.001)과 아연 농도(p<0.01)는 오염구와 비오염구 간에 유의적인 차이가 있었다, 퇴적물의 구리, 납, 아연 및 카드뮴의 농도는 13~55 ㎍/g, 26~101 ㎍/g, 51~263 ㎍/g, 0.8~2.2 ㎍/g 범위를 모였으며, 오염구와 비오염구 사이에 카드뮴 농도는 유의적인 차이를 나타냈다(p<0.05). 진주담치의 구리, 납, 아연 및 카드뮴 농도는 0.69~2.27 ㎍/g, 0.29~1.50 ㎍/g, 13.57~52.90 ㎍/g, 0.09~0.85 ㎍/g 범위로 나타났고, 각 중금속 농도가 오염구와 비오염구 사이에 유의적인 차이를 보이지 않았다. 본 연구에서 오염구에 있는 진주담치의 중금속 농도는 자연 상태인 비오염구에서의 농도와 유사한 값으로 이는 연안 해수가 중금속에 의해 크게 오염되지 않은 것으로 사료된다. This study was carried out to investigate the concentration of Cu, Pb, Zn and Cd in seawater, sediments and Mytilus edulis at Massan Bay, Onsan Bay, Daesan industrial complex and unpolluted area for the degree of contamination on the coast of Korea. The concentrations of dissolved Cu, Pb, Zn and Cd in seawater showed the ranges of 0.29~0.79 ㎍/l, 0.03~0.08 ㎍/l, 0.19~2.01 ㎍/l, 0.01~0.05 ㎍/l, respectively. The concentrations of Pb(p<0.001) and Zn(p<0.01) showed the significant difference between the concentrations of unpolluted area. There were the ranges of 13~55 ㎍/g, 26~101 ㎍/g, 51~263 ㎍/g and 0.8~2.2 ㎍/g in the concentration of Cu, Pb, Zn and Cd of sediments. The concentrations of Cu were significantly different between the contaminated and unpolluted area. The concentration of Cu, Pb, Zn and Cd, in the Mythilus edulis showed the ranges of 0.69~2.27 ㎍/g, 0.29~1.50 ㎍/g, 13.57~52.90 ㎍/g and 0.09~0.85 ㎍/g respectively. These concentrations of trace metal in Mytilus edulis were not significantly different with sites. Trace metal contents of Mytilus edulis in the contaminated site were similar th those in the unpolluted area. This is thought that metal concentration in coastal seawater were not relatively serious.

Blooms of Trichodesmium erythraeum in the South Eastern Arabian Sea during the onset of 2009 Summer Monsoon

K. B. Padmakumar,B. R. Smitha,Lathika Cicily Thomas,C. L. Fanimol,G. SreeRenjima,N. R. Menon,V. N. Sanjeevan 한국해양과학기술원 2010 Ocean science journal Vol.45 No.3

This study presents in situ evidence for the blooms of Trichodesmium erythraeum observed in the shelf waters of the South Eastern Arabian Sea (SEAS) during the onset of the southwest monsoon in June 2009. Evidence showed that water surface discoloration was caused by the accumulation of T. erythraeum, and that the water column contained a colony of T. thiebautii. The surface water color in the bloom region varied from pale brown to pinkish red. Pale brown indicated healthy algae at the peak of its photosynthetic activity, while pinkish red indicated the presence of photosynthetically less active filaments. Zooplankton abundance, especially copepodites, in the bloom area substantiated the theory that Trichodesmium filaments are excellent epiphytes to which the copepodites cling. The bloom area was very fertile with copious quantities of dissolved oxygen (6.85 ml L-1), PO4-P (0.108 μmol L-1) and SiO4 (1.29 μmol L-1). Lower NO3-N (0.028 μmol L-1) values in the bloom area did not appear to affect Trichodesmium growth from molecular nitrogen fixation. However, lower NO3-N values altered the normal phytoplankton composition of this area.

Anti-tumor Promoting Activity of Some Malaysian Traditional Vegetables (Ulam)

L. Yang Mooi,A. M. Ali,A. B. Norhanom,K. Mat Salleh,A. Murakami,K. Koshimizu 한국생약학회 1999 Natural Product Sciences Vol.5 No.1

Ethanolic extracts of different parts of 10 loyal traditional vegetables (ulam) (Amarunthus gangeticus, Jussiaea linifolia, Eugenic polyantha, Trapa incisca, Trichosanthes anquina, Mangifera indica, Pachyrrhirus erosus, Barringtonia mcarostachya, Carica papaya, and Coleus tuberosus) were screened for in vitro anti-tumor promoting activity using the inhibition test of Epstein-Barr virus (EBV) activation in Raji cells induced by phorbol 12-myristate 13-acetate and sodium-n-butyrate. All the extracts were found to have strong inhibition activity toward EBV-activation, except for leaf extract of T. anquina. The extracts were non-cytotoxic to the Raji cells except for the extracts of A. gangeticus (leaves), B. mucrostachya (leaves), E. polyuntha (young leaves), and J. linifolia (leaves) where the viability of the cells were decreased significantly.