Scalable Production of Highly Sensitive Nanosensors Based on Graphene Functionalized with a Designed G Protein-Coupled Receptor

Lerner, Mitchell B.,Matsunaga, Felipe,Han, Gang Hee,Hong, Sung Ju,Xi, Jin,Crook, Alexander,Perez-Aguilar, Jose Manuel,Park, Yung Woo,Saven, Jeffery G.,Liu, Renyu,Johnson, A. T. Charlie American Chemical Society 2014 NANO LETTERS Vol.14 No.5

<P/><P>We have developed a novel, all-electronic biosensor for opioids that consists of an engineered μ-opioid receptor protein, with high binding affinity for opioids, chemically bonded to a graphene field-effect transistor to read out ligand binding. A variant of the receptor protein that provided chemical recognition was computationally redesigned to enhance its solubility and stability in an aqueous environment. A shadow mask process was developed to fabricate arrays of hundreds of graphene transistors with average mobility of ∼1500 cm<SUP>2</SUP> V<SUP>–1</SUP> s<SUP>–1</SUP> and yield exceeding 98%. The biosensor exhibits high sensitivity and selectivity for the target naltrexone, an opioid receptor antagonist, with a detection limit of 10 pg/mL.</P>

Blueberry Intake Alters Skeletal Muscle and Adipose Tissue Peroxisome Proliferator-Activated Receptor Activity and Reduces Insulin Resistance in Obese Rats

E. Mitchell Seymour,Ignasia I. Tanone,Daniel E. Urcuyo-Llanes,Sarah K. Lewis,Ara Kirakosyan,Michael G. Kondoleon,Peter B. Kaufman,Steven F. Bolling 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.12

Metabolic syndrome can precede the development of type 2 diabetes and cardiovascular disease and includes phenotypes such as obesity, systemic inflammation, insulin resistance, and hyperlipidemia. A recent epidemiological study indicated that blueberry intake reduced cardiovascular mortality in humans, but the possible genetic mechanisms of this effect are unknown. Blueberries are a rich source of anthocyanins, and anthocyanins can alter the activity of peroxisome proliferatoractivated receptors (PPARs), which affect energy substrate metabolism. The effect of blueberry intake was assessed in obesity-prone rats. Zucker Fatty and Zucker Lean rats were fed a higher-fat diet (45% of kcal) or a lower-fat diet (10% of kcal)containing 2% (wt/wt) freeze-dried whole highbush blueberry powder or added sugars to match macronutrient and calorie content. In Zucker Fatty rats fed a high-fat diet, the addition of blueberry reduced triglycerides, fasting insulin, homeostasis model index of insulin resistance, and glucose area under the curve. Blueberry intake also reduced abdominal fat mass,increased adipose and skeletal muscle PPAR activity, and affected PPAR transcripts involved in fat oxidation and glucose uptake/oxidation. In Zucker Fatty rats fed a low-fat diet, the addition of blueberry also significantly reduced liver weight, body weight, and total fat mass. Finally, Zucker Lean rats fed blueberry had higher body weight and reduced triglycerides, but all other measures were unaffected. In conclusion, whole blueberry intake reduced phenotypes of metabolic syndrome in obesityprone rats and affected PPAR gene transcripts in adipose and muscle tissue involved in fat and glucose metabolism.

해양수색 위성자료의 검 , 보정

서영상,Mitchell, B.G.,장이현,이삼근,유신재 한국환경과학회 2001 한국환경과학회지 Vol.10 No.6

Variations in phytoplankton concentrations result from changes of the ocean color caused by phytoplankton pigments. Thus, ocean spectral reflectance for low chlorophyll waters are blue and high chlorophyll waters tend to have green reflectance. In the Korea region, clear waters and the open sea in the Kuroshio regions of the East China Sea have low chlorophyll. As one moves even closer to the northwestern part of the East China Sea, the situation becomes much more optically complicated, with contributions not only from higher concentrations of phytoplankton, but also from sediments and dissolved materials from terrestrial and sea bottom sources. The color often approaches yellow-brown in the turbidity waters (Case Ⅱ waters). To verify satellite ocean color retrievals, or to develop new algorithms for complex case Ⅱ regions requires ship-based studies. In this study, we compared the chlorophyll retrievals from NASA's SeaWiFS sensor with chlorophyll values determined with standard fluorometric methods during two cruises on Korean NFRDI ships. For the SeaWiFS data, we used the standard NASA SeaWiFS algorithm to estimate the chlorophyll a distribution around the Korean waters using Orbview/ SeaWiFS satellite data acquired by our HPRT station at NFRDI. We studied to find out the relationship between the measured chlorophyll a from the ship and the estimated chlorophyll a from the SeaWiFS satellite data around the northern part of the East China Sea, in February, and May, 2000. The relationship between the measured chlorophyll_a and the SeaWiFS chlorophyll_a shows following the equations (1) in the northern part of the East China Sea. Chlorophyll_a=0.121Ln(X) + 0.504, R^2 = 0.73 (1) We also determined total suspended sediment mass (SS) and compared it with SeaWiFS spectral band ratio. A suspended solid algorithm was composed of in-situ data and the ratio (L_WN(490 nm)/L_WN(555 nm)) of the SeaWiFS wavelength bands. The relationship between the measured suspended solid and the SeaWiFS band ratio shows following the equation (2) in the northern part of the East China Sea. SS=-0.703 Ln(X) + 2.237, R^2 = 0.62 (2) In the near future, NFRDI will develop algorithms for quantifying the ocean color properties around the Korean waters, with the data from regular ocean observations using its own research vessels and from three satellites, KOMPSAT/OSMI, Terra/MODIS and Orbview/SeaWiFS.

위성의 해색 영상과 해수면온도 영상을 활용한 재발생 와동류에 관한 연구

서영상 ( Young Sang Suh ),B. G. Mitchell,임근식 ( Kyuen Sik Lim ) 大韓遠隔探査學會 1999 大韓遠隔探査學會誌 Vol.15 No.2

동한 난류의 북상 끝부분 해역에서 재발생하는 와동류는 봄철과 가을철에 위성이 관측한 해수면 온도 영상에 나타났다. 이러한 재발생 와동류는 1997년 9월 하순 NOAA 위성에 탑재된 AVHRR의 열적외선 영상과 일본 ADEOS 위성에 탑재된 OCTS의 클로로필 영상에도 나타났다. 와동류의 중심은 주변보다 온도가 낮으며, OCTS 위성자료에서 3 mg/m3 이상의 클로로필 농도가 나타났다. 반면, 와동류를 이루는 주변의 더운물에서는 클로로필 농도가 1mg/m3 이하로 나타났다. 와동류는 가을철 표면수온영상에도 나타났으며 봄에 나타난 것보다 와동류 중심핵의 찬물 온도가 높게 나타났다. 또한 와동류 중심에서 서쪽축의 더운물 온도가 봄에 나타난 것보다 가을에 더 높게 나타났다. 1998년 3월 NOAA위성과 SeaWiFS 위성 영상자료에서도 재발생되는 와동류와 클로로필량의 농도가 높은 물이 와동류 주변으로 포힉되는 장면이 포착되었다. 동한난류의 북쪽 확장 선두와 약 1500m 수심의 대륙붕 위로 남하하는 리만한류가 만나 충돌하는 해역에서 와동류가 형성되는 것으로 사료된다. 와동류가 형성되는 이 해역은 동해 중앙부 해수면에서 우세하게 나타나는 극전선역에서 중규모 구조의 와동류가 극전선 서쪽해역의 역학적인 해양현상과 강한 연관성이 있음을 나타내었다. ARGOS 위성추적 표류부이와 와동류의 상호연관성 및 와동류의 지속성에 관한 증거가 토론되었다. A recurring eddy which located at the terminal end of the Korean East Warm Current was captured on ocean color and sea surface temperature imagery from satellite in spring and autumn. During late April, 1997 thermal infrared imagery from the NOM AVHRR sensor and ocean color data from the Japanese ADEOS-1 OCTS sensor, revealed this feature. The cold core had elevated chlorophyll concentrations, based on OCTS estimates, of greater than 3 mg/m3 while the warmer surrounding waters had chlorophyll concentrations of 1 mg/m3 or less. The elevated chlorophyll associated with this eddy has not been previously described. The eddy is also evident in SST images from autumn, but the SST in the core is warmer than in spring, and the warm jet flowing to the west of the eddy is also warmer in autumn compared to spring. A reccuring eddy and the high chlorophyll_a concentration area which surround around the eddy show on NOAA and SeaWiFS images in March 2, 1998. The eddy forms at the northern extent of the Korean East Warm Current as those waters collide with the cold, south-flowing Liman Current over a topographic shelf about 1500 m deep. This region of the eddy formation appears to have a strong connection with the dynamics of the western part of the polar front eddy field that dominates surface mesoscale structure in the central East (Japan) Sea. Interaction of the eddy with ARGOS tracked drifters, and evidence for its persistence are discussed.

The ocean color properties of the northern part of the East China Sea

Suh, Young Sang,Mitchell, B. G.,Lee, Sam Guen,Jang, Lee Hyun 대한원격탐사학회 2000 International Symposium on Remote Sensing Vol.16 No.1

We compared the chlorophyll retrievals from NASA's SeaWiFS sensor with chlorophyll values determined with standard fluorometric methods in two Korean NFRDI cruises. For the SeaWiFS data, we used the standard NASA SeaWiFS algorithm to estimate the chlorophyll a distribution around the Korean waters using Orbview/ SeaWiFS satellite data (McClain, 1997) acquired by the HRPT station at NFRDI. We studied to find out the relationship between the measured chlorophyll a from the ship and the estimated chlorophyll a from the SeaWiFS satellite data around the northern part of the East China Sea, in February, and May, 2000. We also determined total suspended sediment mass (SS) and compared it with SeaWiFS (OSMI) spectral band ratio. A suspended solid algorithm was composed with in-situ data obtained in the study areas and remote sensing reflectance obtained from the ratio (nLw(490 nm)/nLw(555 nm)) of the SeaWiFS (OSMI) wavelength bands. Furthermore, we described the relationship between remote sensing reflectance and chlorophyll a. In the near future, we will develop algorithms for quantifying the ocean color properties around the Korean Waters, with the data from regular ocean observations using NFRDI research vessels and from the three satellites, KOMPSAT/OSMI, Terra/MODIS and Orbview/SeaWiFS.

Study on the Korean Waters using the CAL/VAL of the OSMI Level 2 Data

Young Sang Suh,Hyun Jang Lee,B. G. Mitchell,M. Kahru,Kota Prasad,H. Y. Shin 大韓遠隔探査學會 2002 大韓遠隔探査學會誌 Vol.18 No.3

A comparison was made between the chlorophyll a and suspended solid (SS) retrievals from OSMI and SeaWiFS sensor to chlorophyll a and SS values determined with the standard method during the NFRDI`s research cruises. The percentage of organic and inorganic materials from the SS was calculated to study the contribution of turbid water in the northern part of the East China Sea. The open sea waters in the Kuroshio regions of the East China Sea showed relatively higher concentration of volatile SS. However, towards the northwestern part of the East China Sea, the situation became much more optically different with the non-volatile SS from the Yangtze river and the sea bottom sources in the sea in winter and spring seasons. Furthermore, in order to indirectly detect low salinity water with high turbidity, which related to the Yangtze river using remote sensed data from the satellites, a comparison between the results of the band ratio(nLw 490nm/nLw 555nm) of SeaWiFS (OSMI) and the distribution of low salinity around the Jeju Island was presented.

The Non-Surgical Treatment of Peyronie Disease: 2013 Update

Eric James Shaw,Gregory Clyde Mitchell,Ronny B. Tan,Premsant Sangkum,Wayne John G. Hellstrom 대한남성과학회 2013 The World Journal of Men's Health Vol.31 No.3

Peyronie disease is a common cause of penile deformity and sexual dysfunction. Although surgery is regarded as the definitive management for this condition, there are many medical and minimally invasive therapies available, with widely varying efficacy reported in the literature. The purpose of this review is to describe the current state-of-the-art for each of the most commonly used as well as several developing non-surgical treatments. Further, we hope to offer perspectives that will aid practitioners in deciding among these treatments that are either already in use or have the potential to be used as alternatives to surgery in the management of this frustrating disease.

Study on the Korean Waters using the CAL/VAL of the OSMI Level 2 Data

Suh, Young-Sang,Jang, Lee-Hyun,Mitchell, B.G.,Kahru, M.,Prasad, Kota,Shin, H.Y. The Korean Society of Remote Sensing 2002 大韓遠隔探査學會誌 Vol.18 No.3

A comparison was made between the chlorophyll $\alpha$ and suspended solid (SS) retrievals from OSMI and SeaWiFS sensor to chlorophyll $\alpha$ and SS values determined with the standard method during the NFRDI's research cruises. The percentage of organic and inorganic materials from the SS was calculated to study the contribution of turbid water in the northern part of the East China Sea. The open sea waters in the Kuroshio regions of the East China Sea showed relatively higher concentration of volatile SS. However, towards the northwestern part of the East China Sea, the situation became much more optically different with the non-volatile SS from the Yangtze river and the sea bottom sources in the sea in winter and spring seasons. Furthermore, in order to indirectly detect low salinity water with high turbidity, which related to the Yangtze river using remote sensed data from the satellites, a comparison between the results of the band ratio(nLw 490nm/nLw 555nm) of SeaWiFS(OSMI) and the distribution of low salinity around the Jeju Island was presented.

Sex Determination of Mouse Preimplantation Embryo by Blastomere Biopsy and PCR

( J. H. Chung ),( S. D. Shim ),( D. S. Choi ),( B. K. Yoon ),( D. S. Bae ),( J. H. Lee ),( R. V. Lebo ),( G. S. Mitchel ) 대한산부인과학회 1994 대한산부인과학회 학술대회 Vol.74 No.-

THE <i>COSMIC INFRARED BACKGROUND EXPERIMENT</i> ( <i>CIBER</i> ): THE WIDE-FIELD IMAGERS

Bock, J.,Sullivan, I.,Arai, T.,Battle, J.,Cooray, A.,Hristov, V.,Keating, B.,Kim, M. G.,Lam, A. C.,Lee, D. H.,Levenson, L. R.,Mason, P.,Matsumoto, T.,Matsuura, S.,Mitchell-Wynne, K.,Nam, U. W.,Renbarg IOP Publishing 2013 The Astrophysical journal, Supplement series Vol.207 No.2