[P8-300] Immune-Activation and Antioxidant Effects of Polysaccharide Recovered from Processing By-product of Ecklonia cava

Areum-Daseul Kim,Ginnae Ahn,Youngheun Jee,You-Jin Jeon 한국식품영양과학회 2008 한국식품영양과학회 학술대회발표집 Vol.2008 No.10

Effects of Jeju Water Containing Vanadium on Antioxidant Enzymes in vitro

Areum Daseul Kim,Kyoung Ah Kang,Rui Zhang,Mei Jing Piao,김석만,지영흔,Nam Ho Lee,Ho Jin You,고경수,현진원 대한암예방학회 2010 Journal of cancer prevention Vol.15 No.4

scavenging of reactive oxygen species (ROS). In the present study, the aim was to examine the antioxidant effect of Jeju water containing vanadium (S3, 26.0±2.0 μg/l) in terms of antioxidant enzymes in vitro. Human Chang liver cells were incubated for 10 passages in media prepared with either deionized distilled water (DDW) or S3. S3 increased activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and heme oxygenase-1 (HO-1) compared to DDW. S3 also increased the level of reduced glutathione (GSH) shown by colorimetric assay and by high-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy. These results suggest that vanadium-containing Jeju water (S3) produces antioxidant effect in vitro by enhancing antioxidant enzyme activities. (Cancer Prev Res 15, 262-267, 2010)

Reactive Oxygen Species Scavenging Effects of Jeju Waters Containing Vanadium Components

Areum Daseul Kim,Kyoung Ah Kang,Rui Zhang,임채문,Youngheun Jee,이남호,Ho Jin You,고경수,현진원 대한암예방학회 2010 Journal of cancer prevention Vol.15 No.2

The aim of this study was to examine the reactive oxygen species (ROS) scavenging effects of Jeju waters containing vanadium components; S1 (8.0±0.9μg/l), S2 (24.0±2.0μg/l), and S3 (26.0±2.0μg/l). Human Chang liver cells were incubated for 10 passages in media containing deionized distilled water (DDW group) and Jeju waters (S1, S2, and S3 groups). DDW and Jeju waters did not show cytotoxicity and scavenging effect of 1,1-diphenyl-2-picrylhydrazyl radical. Electron spin resonance spectrometer data showed that cells exposed to Jeju waters showed significantly scavenged superoxide anion induced by the xanthine/xanthine oxidase system and hydroxyl radicals induced by Fenton reaction (H2O2+FeSO4)compared to cells exposed to DDW. Furthermore, the S3 group significantly scavenged intracellular ROS compared to the DDW group, as measured by spectrofluorometry, flow cytometry, and confocal microscopy after staining with 2',7'-dichlorodihydrofluorescein diacetate. These results suggest that vanadium components-containing Jeju waters exhibited antioxidant effect by scavenging ROS. (Cancer Prev Res 15, 111-117, 2010)

Involvement of glutathione and glutathione metabolizing enzymes in human colorectal cancer cell lines and tissues

KIM, AREUM DASEUL,ZHANG, RUI,HAN, XIA,KANG, KYOUNG AH,PIAO, MEI JING,MAENG, YOUNG HEE,CHANG, WEON YOUNG,HYUN, JIN WON SPANDIDOS PUBLICATIONS 2015 MOLECULAR MEDICINE REPORTS Vol.12 No.3

<P>Reduced glutathione (GSH) is an abundant tripeptide present in the majority of cell types. GSH is highly reactive and is often conjugated to other molecules, via its sulfhydryl moiety. GSH is synthesized from glutamic acid, cysteine, and glycine via two sequential ATP?consuming steps, which are catalyzed by glutamate cysteine ligase (GCL) and GSH synthetase (GSS). However, the role of GSH in cancer remains to be elucidated. The present study aimed to determine the levels of GSH and GSH synthetic enzymes in human colorectal cancer. The mRNA and protein expression levels of GSH, the catalytic subunit of GCL (GCLC) and GSS were significantly higher in the following five colon cancer cell lines: Caco?2, SNU?407, SNU?1033, HCT?116, and HT?29, as compared with the normal colon cell line, FHC. Similarly, in 9 out of 15 patients with colon cancer, GSH expression levels were higher in tumor tissue, as compared with adjacent normal tissue. In addition, the protein expression levels of GCLC and GSS were higher in the tumor tissue of 8 out of 15, and 10 out of 15 patients with colon cancer respectively, as compared with adjacent normal tissue. Immunohistochemical analyses confirmed that GCLC and GSS were expressed at higher levels in colon cancer tissue, as compared with normal mucosa. Since GSH and GSH metabolizing enzymes are present at elevated levels in colonic tumors, they may serve as clinically useful biomarkers of colon cancer, and/or targets for anti-colon cancer drugs.</P>

Consumption of Jeju Ground Water Containing Vanadium Components Enhances Hepatic Antioxidant Defense Systems in ob/ob Mice

Areum Daseul Kim(김아름다슬),Ho Jin You(유호진),Jin Won Hyun(현진원) 한국생명과학회 2012 생명과학회지 Vol.22 No.1

비만 마우스의 산화적 스트레스에 대해 바나듐 함유 제주 지하수에 대한 연구로서, 제주 지하수는 수도수 처리군보다 비만 마우스 간의 과산화 지질을 감소시켰으며, superoxide dismutase, catalase, 그리고 glutathione peroxidase의 단백질 발현 및 활성 그리고 환원형 glutathione 양을 증가 시켰다. 제주 지하수는 항산화 효소의 전사인자인 erythroid transcription factor NF-E2 (Nrf2)의 인산화형 단백질 발현을 증가시켰다. 이로서 제주 지하수는 Nrf2의 활성화를 통하여 항산화 효소시스템을 증가시켰다. The present study examined the effects of consumption of Jeju ground water containing vanadium components on oxidative stress in obese (ob/ob) mice. Intake of Jeju ground water decreased the generation of oxidative stress induced-lipid peroxidation in the liver of ob/ob mice It also enhanced the enzymatic antioxidant defense system by increasing the protein expression and activity of superoxide dismutase, catalase, and glutathione peroxidase in liver tissues. Jeju ground water intake also upregulated the intracellular content of reduced glutathione. The induction of antioxidant enzyme expression by consumption of Jeju ground water was mediated by the erythroid transcription factor NF-E2 (Nrf2). Increased nuclear expression of phospho Nrf2 was observed in ob/ob mouse liver cells following intake of Jeju ground water. These results suggest that consumption of Jeju ground water stimulated the antioxidant defense system in the livers of ob/ob mice via induction of Nrf2.

Antioxidant System-Inducing Effects of Jeju Ground Water in C57BL,6 Mice against Gamma-ray Radiation

( Areum Daseul Kim ),( Youngheun Jee ),( Ho Jin You ),( Jin Won Hyun ) 한국응용생명화학회 2012 Journal of Applied Biological Chemistry (J. Appl. Vol.55 No.1

Recently, we reported that Jeju ground water contains vanadium components and exerts antioxidant effects in vitro and in vivo via the scavenging of reactive oxygen species and enhancement of antioxidant enzyme activities. In the present study, the antioxidant actions of Jeju ground water were compared with those of tap water against gamma-ray radiation in mice. C57BL/6 mice were irradiated with gamma-ray at a dose rate of 2 Gy. The mice were then given tap water or Jeju ground water for 90 days. Jeju ground water compared with tap water enhanced the activities and levels of superoxide dismutase, catalase, and glutathione peroxidase in irradiated liver tissues. Jeju ground water also enhanced the levels of intracellular reduced glutathione, which is vital for normal liver function and repair. These results suggest that vanadium-containing Jeju ground water can safeguard against the harmful actions of gamma-ray radiation through the support of hepatic antioxidant processes.

Photo-protective Properties of Lomentaria hakodatensis Yendo against Ultraviolet B Radiation-induced Keratinocyte Damage

Areum Daseul Kim,Mei Jing Piao,Yu Jae Hyun,강희경,In Soo Suh,이남호,현진원 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.6

This study aims to investigate the photoprotective properties of a Lomentaria hakodatensis ethanol extract (LHE) against ultraviolet B (UVB) radiationinduced cellular damage in human HaCaT keratinocytes. LHE exhibited scavenging activity against intracellular reactive oxygen species (ROS), which were generated by either hydrogen peroxide (H2O2) or UVB radiation. Moreover, LHE scavenged superoxide anion generated by the xanthine/xanthine oxidase system and hydroxyl radical generated by the Fenton reaction (FeSO4 + H2O2). Furthermore, LHE exhibited UVB absorptive properties and attenuated injury to cellular components (e.g., lipids,proteins and DNA), resulting from UVB-induced oxidative stress. In addition, LHE reduced apoptosis in response to UVB, as shown by decreased DNA fragmentation and the formation of apoptotic bodies. These results suggest that LHE protects human keratinocytes against UVB-induced oxidative stress by scavenging ROS and absorbing UVB rays; thereby reducing damage to biological components.

Increased Glutathione Synthesis Following Nrf2 Activation by Vanadyl Sulfate in Human Chang Liver Cells

Kim, Areum Daseul,Zhang, Rui,Kang, Kyoung Ah,You, Ho Jin,Hyun, Jin Won Molecular Diversity Preservation International (MD 2011 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.12 No.12

<P>Jeju ground water, containing vanadium compounds, was shown to increase glutathione (GSH) levels as determined by a colorimetric assay and confocal microscopy. To investigate whether the effects of Jeju ground water on GSH were specifically mediated by vanadium compounds, human Chang liver cells were incubated for 10 passages in media containing deionized distilled water (DDW), Jeju ground water (S1 and S3), and vanadyl sulfate (VOSO<SUB>4</SUB>). Vanadyl sulfate scavenged superoxide anion, hydroxyl radical and intracellular reactive oxygen species. Vanadyl sulfate effectively increased cellular GSH level and up-regulated mRNA and protein expression of a catalytic subunit of glutamate cysteine ligase (GCLC), which is involved in GSH synthesis. The induction of GCLC expression by vanadyl sulfate was found to be mediated by transcription factor erythroid transcription factor NF-E2 (Nrf2), which critically regulates GCLC by binding to the antioxidant response elements (AREs). Vanadyl sulfate treatment increased the nuclear translocation of Nrf2 and the accumulation of phosphorylated Nrf2. Extracellular regulated kinase (ERK) contributed to ARE-driven GCLC expression via Nrf2 activation. Vanadyl sulfate induced the expression of the active phospho form of ERK. Taken together, these results suggest that the increase in GSH level by Jeju ground water is, at least in part, due to the effects of vanadyl sulfate via the Nrf2-mediated induction of GCLC.</P>

Dictyopteris undulata Extract Induces Apoptosis in Human Colon Cancer Cells

Kim, Areum Daseul,Kang, Kyoung Ah,Piao, Jing Mei,Kim, Ki Cheon,Zheng, Jian,Yao, Cheng Wen,Cha, Ji Won,Hyun, Chang Lim,Boo, Sun Jin,Lee, Nam Ho,Na, Soo Young,Hyun, Jin Won 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.3

The present study investigated the cytotoxic and apoptotic effects of an ethanol extract derived from the marine brown alga Dictyopteris undulata against human colon adenocarcinoma cells. The Dictyopteris undulata extract (DUE) showed cytotoxic activity against SW480 cells in a dose-dependent manner, with 50% inhibition of cell viability at a concentration of $40{\mu}g/mL$. DUE also induced programmed cell death in SW480 cells, as evidenced by apoptotic body formation, DNA fragmentation, an increase in the population of apoptotic sub-$G_1$ phase cells, and mitochondrial membrane depolarization. Moreover, DUE significantly modulated the expression of apoptosis-associated proteins, resulting in a decrease in B cell lymphoma-2 expression and an increase in Bcl-2-associated X protein expression, as well as the activation of caspase-9 and caspase-3. Furthermore, DUE showed apoptotic cell death in two other colon cancer cell lines, SNU407 and HT29. These observations suggest that DUE may prove useful as a therapeutic agent for the attenuation of colon cancer.

Potential Antioxidant Activities of Enzymatic Digests from Fresh Water Microalgae, Pediastrum duplex and Dactylococcopsis fascicularis

이승홍,Areum-Daseul Kim,강민철,이준백,You-Jin Jeon 한국조류학회I 2009 ALGAE Vol.24 No.3

In this study, we focused on natural water-soluble antioxidants from fresh water microalgae, Pediastrum duplex and Dactylococcopsis fascicularis from Jeju Island, Korea. They were prepared by enzymatic digestion using five carbohydrases (Viscozyme, Celluclast, AMG, Termamyl and Ultraflo) and five proteases (Protamex, Kojizyme, Neutrase, Flavourzyme and Alcalase), and the potential antioxidant activity of each was assessed. All enzymatic digests from P. duplex showed significant DPPH scavenging effects. Termamyl (60.6%) digest from P. duplex possessed the highest effects on hydrogen peroxide scavenging. Celluclast (58.1%) and Kojizyme digests (56.9%) from D. fascicularis possessed higher effects on superoxide anion radical scavenging. All enzymatic digests exhibited significant effects on both NO· scavenging and metal chelating. Lipid peroxidation was significantly in inhibited Viscozyme, Termamyl and Kojizyme digests from P. duplex and Ultraflo, Protamex, Kojizyme and Alcalase digests from D. fascicularis. These data suggest that enzymatic digests of the fresh water microalgae, P. duplex and D. fascicularis might be valuable sources of antioxidant which can be applied in food and pharmaceutical industry.