Parthenogenetic Activation of Black Bengal Goat Oocytes

Haque, Aminul,Bhuiyan, Mohammad Musharraf Uddin,Khatun, Momena,Shamsuddin, Mohammed 韓國受精卵移植學會 2011 한국동물생명공학회지 Vol.26 No.2

In vitro maturation and activation of oocytes are primary steps towards biotechnological manipulation in embryology. The objectives of the present study were to determine the oocyte recovery rate per ovary, in vitro maturation rates of oocytes and rates of parthenogenetically activation of matured oocytes in Black Bengal goats. All visible follicles were aspirated to recover follicular fluid from individual ovaries (number of ovaries = 456). The immature cumulus oocyte complexes (COCs; n = 1289) were cultured in tissue culture medium (TCM)-199 supplemented with 10% (v/v) fetal bovine serum (FBS) for 27 hours at with 5% in humidified air. The matured oocytes (n = 248) were activated with 5 ionomycin for 5 minutes followed by treatment with 2 mM 6-dimethylaminopurine (6-DMAP) for 4 hours. After activation, oocytes were cultured for another 14 hours in TCM-199 supplemented with bovine serum albumin (BSA) at with 5% in humidified air. The pronucleus formation in activated oocytes was determined by staining with 1% orcein (whole mount technique). Matured oocytes (n = 176) without activation stimuli were used as control. The mean number of oocytes recovered per ovary was . The proportion of oocytes matured in vitro, confirmed by the presence of first polar body, was . Parthenogenetic activation, evidenced by formation of pronucleus, occurred in of matured oocytes. No pronucleus formation was observed in control oocytes. In conclusion, a combination of ionomycin and 6-DMAP induces activation in one third of Black Bengal goats' oocytes.

Parthenogenetic Activation of Black Bengal Goat Oocytes

Aminul Haque,Mohammad Musharraf Uddin Bhuiyan,Momena Khatun,Mohammed Shamsuddin 사단법인 한국동물생명공학회 2011 한국동물생명공학회지 Vol.26 No.2

In vitro maturation and activation of oocytes are primary steps towards biotechnological manipulation in embryology. The objectives of the present study were to determine the oocyte recovery rate per ovary, in vitro maturation rates of oocytes and rates of parthenogenetically activation of matured oocytes in Black Bengal goats. All visible follicles were aspirated to recover follicular fluid from individual ovaries (number of ovaries = 456). The immature cumulus oocyte complexes (COCs; n = 1289) were cultured in tissue culture medium (TCM)-199 supplemented with 10% (v/v) fetal bovine serum (FBS) for 27 hours at 39℃ with 5% CO_2 in humidified air. The matured oocytes (n =248) were activated with 5 μM ionomycin for 5 minutes followed by treatment with 2 mM 6-dimethylaminopurine (6-DMAP) for 4 hours. After activation, oocytes were cultured for another 14 hours in TCM-199 supplemented with bovine serum albumin (BSA) at 39℃ with 5% CO_2 in humidified air. The pronucleus formation in activated oocytes was determined by staining with 1% orcein (whole mount technique). Matured oocytes (n = 176) without activation stimuli were used as control. The mean number of oocytes recovered per ovary was 3.5 ± 0.5. The proportion of oocytes matured in vitro, confirmed by the presence of first polar body, was 42.1 ± 4.7%. Parthenogenetic activation,evidenced by formation of pronucleus, occurred in 37.2 ± 15.8% of matured oocytes. No pronucleus formation was observed in control oocytes. In conclusion, a combination of ionomycin and 6-DMAP induces activation in one third of Black Bengal goats’ oocytes

Effect of Crop Establishment and Weed Control Method on Productivity of Transplanted aman Rice

Ali, Mohammad,Haque Bir, Md. Shahidul,Rahman, Md. Habibur,Ayesha, Sultana Kaniz,Hoque, Aminul,Harun-Ar-Rashid, Md.,Islam, Md. Rashidul,Park, Kee Woong The Korean Society of Weed ScienceThe Turfgrass So 2018 Weed & Turfgrass Science Vol.7 No.2

This experiment was conducted to find the most suitable crop establishment method and weed management practices for transplanted aman rice in Bangladesh. Rice variety Bangladesh Rice Research Institute (BRRI) dhan44 was used as planting materials where three crop establishment methods ($T_1$: direct wet seeding by drum seeder; $T_2$: hand broadcasting; $T_3$: transplanting) and four weeding options ($W_1$: Hand weeding (HW); $W_2$: BRRI weeder+HW; $W_3$: Herbicide+HW; and $W_4$: no weeding) were tested. Among the crop establishment methods, the highest grain yield ($5.12t\;ha^{-1}$) was obtained with the $T_3$, while the highest benefit cost ratio (BCR) of 2.08 was found in $T_2$. In case of the weed management method, $W_1$ showed superior results on the plant $density/m^2$ (139.66) at 60 days after transplanting (DAT), grain yield ($4.97t\;ha^{-1}$), and BCR (2.03). On the other hand, the highest plant dry matter (36.20 g) at 60 DAT and the highest yield ($6.10t\;ha^{-1}$) were obtained in a $T_3W_1$ combination. The results of this study show that the productivity of rice during aman season could be most significantly increased with the use of transplanting ($T_3$) alone, hand weeding ($W_1$) alone, or a combination of the two methods ($T_3W_1$).

Effect of Crop Establishment and Weed Control Method on Productivity of Transplanted aman Rice

Mohammad Ali,Md. Shahidul Haque Bir,Md. Habibur Rahman,Sultana Kaniz Ayesha,Aminul Hoque,Md. Harun-Ar-Rashid,Md. Rashidul Islam,박기웅 한국잔디학회 2018 Weed & Turfgrass Science Vol.7 No.2

This experiment was conducted to find the most suitable crop establishment method and weed management practices for transplanted aman rice in Bangladesh. Rice variety Bangladesh Rice Research Institute (BRRI) dhan44 was used as planting materials where three crop establishment methods (T1: direct wet seeding by drum seeder; T2: hand broadcasting; T3: transplanting) and four weeding options (W1: Hand weeding (HW); W2: BRRI weeder+HW; W3: Herbicide+HW; and W4: no weeding) were tested. Among the crop establishment methods, the highest grain yield (5.12 t ha-1) was obtained with the T3, while the highest benefit cost ratio (BCR) of 2.08 was found in T2. In case of the weed management method, W1 showed superior results on the plant density/m2 (139.66) at 60 days after transplanting (DAT), grain yield (4.97 t ha-1), and BCR (2.03). On the other hand, the highest plant dry matter (36.20 g) at 60 DAT and the highest yield (6.10 t ha-1) were obtained in a T3W1 combination. The results of this study show that the productivity of rice during aman season could be most significantly increased with the use of transplanting (T3) alone, hand weeding (W1) alone, or a combination of the two methods (T3W1).

Genetic diversity analysis of high yielding rice (Oryza sativa) varieties cultivated in Bangladesh

Isma Akter Epe,Shahidul Haque Bir,Abul Kashem Choudhury,Asma Khatun,Most Mohshina Aktar,Shamsul Arefin,Mohammed Aminul Islam,Kee Woong Park 충남대학교 농업과학연구소 2021 Korean Journal of Agricultural Science Vol.48 No.2

Oleanane triterpenoids from Akebiae Caulis exhibit inhibitory effects on Ab42 induced fibrillogenesis

Md. Anisuzzaman Chowdhury,고해주,이환,Md. Aminul Haque,박일선,이동성,우은란 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.3

Previous phytochemical investigations of Akebiae compound 1 and the inhibitory effects of components fromAkebiae Caulis on Ab42 fibrillogenesis. Caulis resulted in the isolation of triterpenes, triterpeneglycosides, phenylethanoid glycosides and megastigmaneglycoside. Amyloid beta (Ab), the main component of thesenile plaques detected in Alzheimer’s disease, induces celldeath. However, only a limited number of studies haveaddressed the biological and pharmacological effects ofAkebiae Caulis. In particular, the inhibitory activity ofAkebiae Caulis against Ab42 fibrillogenesis remainsunclear. Herein, a new triterpene glycoside, akequintoside F(1), along with nine known compounds pulsatilla saponin A(2), collinsonidin (3), akebonic acid (4), hederagenin (5),1-(30,40-dihydroxycinnamoyl) cyclopentane-2,3-diol (6),asperosaponin C (7), leontoside A (8), quinatic acid (9), andquinatoside A (10) were isolated from Akebiae Caulis usingrepeated column chromatography with silica gel, LiChroprepRP-18, and MCI gel. The chemical structures ofcompounds 1–10 were illustrated based on 1D and 2D NMRspectroscopy, including 1H-1H COSY, HSQC, HMBC andNOESY spectroscopic analyses. Compound 1 a novelcompound and known compounds 6 and 7 were isolated forthe first time from this plant. Among these compounds, 1, 3,4, 5 and 7 displayed significant inhibitory effects on Ab42induced fibrillogenesis. We present the first report of new

Variety and Seedling Age Affects Fine Rice Yield

Amin, A.K.M. Khusrul,Haque, M. Aminul,Akhtaruzzaman, Md,Chowdhury, Nazmun Nahar The Korean Society of Crop Science 2007 Korean journal of crop science Vol.52 No.2

The research was conducted with the aim of determining effects of seedling age on the yield of four fine rice varieties viz., Kalizura, Tulshimala, BRRI (Bang-ladesh Rice Research Institute)-37 and BRRI-38. The seedling of different ages such as 15, 25, 35 and 45 days were transplanted on the same day maintaining $25cm{\times}15cm$ spacing. The experiment was laid out in a randomized complete block design with three replications. The yield and yield contributing characters were influenced by seedling age, variety and their interaction. BRRI-38 gave the highest number of effective tillers $hill^{-1}$, panicle length, total spikelets $panicle^{-1}$, grains $panicle^{-1}$, 1000-grains weight and grain yield. Likewise, yield and yield contributing characters were the highest in 35 days old seedling. On the other hand, the variety (BRRI-38) with the same age as of seedlings 35 days old seedlings was found superior to other interactions, but, in the production of grains $panicle^{-1}$ and 1000-grains weight there was no significant effect in this interaction. From the findings it may be inferred that BRRI-38 with 35 days old seedlings produced the highest grain yield.

Variety and Seedling Age Affects Fine Rice Yield

A. K. M. Khusrul Amin,M. Aminul Haque,Md Akhtaruzzaman,Nazmun Nahar Chowdhury 韓國作物學會 2007 Korean journal of crop science Vol.52 No.2

The research was conducted with the aim of determining effects of seedling age on the yield of four fine rice varieties viz., Kalizura, Tulshimala, BRRI (Bang-ladesh Rice Research Institute)-37 and BRRI-38. The seedling of different ages such as 15, 25, 35 and 45 days were transplanted on the same day maintaining 25cm~times15cm spacing. The experiment was laid out in a randomized complete block design with three replications. The yield and yield contributing characters were influenced by seedling age, variety and their interaction. BRRI-38 gave the highest number of effective tillers hill-1 , panicle length, total spikelets panicle-1 , grains panicle-1 , 1000-grains weight and grain yield. Likewise, yield and yield contributing characters were the highest in 35 days old seedling. On the other hand, the variety (BRRI-38) with the same age as of seedlings 35 days old seedlings was found superior to other interactions, but, in the production of grains panicle-1 and 1000-grains weight there was no significant effect in this interaction. From the findings it may be inferred that BRRI-38 with 35 days old seedlings produced the highest grain yield.

In vitro maturation and fertilization of prepubertal and pubertal black Bengal goat oocytes

Momena Khatun,Mohammad Musharraf Uddin Bhuiyan,Jalal Uddin Ahmed,Aminul Haque,Mohammed Shamsuddin,Mohammad Bozlur Rahman 대한수의학회 2011 Journal of Veterinary Science Vol.12 No.1

Oocytes retrieval, in vitro maturation (IVM) and fertilization (IVF) efficiency are inevitable steps towards in vitro production of embryos. In the present study, these parameters were investigated in the ovaries of prepubertal (n = 31) and pubertal (n = 61) black Bengal goats obtained from a slaughterhouse. Nuclear maturation was evaluated upon aspiration and following IVM in TCM-199 (Earle’s salt with L-glutamine and sodium bicarbonate) for 27 h at 39oC under 5% CO2 in humidified air. The oocytes retrieval and efficiency (mean ± SD) per prepubertal and pubertal goats were 5.2 ± 0.6 and 6.8 ± 0.6, and 77.3 ± 0.1% and 80.5 ± 0.6%, respectively. Anaphase I - telophase I stages differed significantly (7.3 ± 0.8 vs. 2.6 ± 0.2, p < 0.05) between the two groups of goats. After IVM, the percentages of metaphase II were significantly higher (66.3 vs. 60.3, p < 0.05) in pubertal goats than in their prepubertal counterparts. The percentages of normal in vitro fertilization (IVF) in Fert-Tyrode’s albumin lactate pyruvate of pubertal goat oocytes did not differ between Percoll and swim-up sperm separation methods (36.7 ± 0.9% vs. 32.7 ± 1.3%, p > 0.05). Furthermore, sperm capacitation by heparin alone or in combination with ionomycin did not lead to a significant increase in the normal fertilization rate (34.8 ± 1.7 vs. 32.2 ± 1.5%, respectively) in the oocytes of pubertal goats. In conclusion, the ovaries of pubertal black Bengal goats obtained from the slaughterhouse could be used for in vitro embryo production. However, further optimization of the IVM and IVF techniques are necessary for satisfactory in vitro embryo production.