Genetic Transformation of Pigeonpea (Cajanus cajan L.) and Screening Transgenic Progenies Based on Lateral Root Inhibition

Alok Das,Manoj Kumar,Arvind Kumar Singh,Alok Shukla,Jamal Ansari,Subhojit Datta,Narendra Pratap Singh 한국작물학회 2016 Journal of crop science and biotechnology Vol.19 No.4

Production of transgenic pigeonpea is becoming increasingly important, but the methods currently employed in production and subsequent screening still requires improvement. Here, we describe Agrobacterium-mediated genetic transformation of pigeonpea with reporter uidA (gus) gene and selectable marker, neomycin phospho-transferase (nptII) gene. Histochemical assay demonstrate localization of gus activity in cells and transformed plants. Overall, a transformation frequency of 0.33% was achieved using the protocol. Grafting of in vitro-regenerated healthy shoots indicates higher survival percent (72.6%), when stock and scion are of the same variety. Seeds harvested from primary transgenic plants can be screened based on lateral root inhibition strategy. Approximately 87% of the screened T1 plants were found to be PCR positive. In conclusion, in vitro grafting of transgenic pigeonpea shoots leads to better plant establishment and screening based on lateral root inhibition leads to quick identification of positive segregants.

Experimental and simulation study of magnetorheological miniature gear-profile polishing (MRMGPP) method using flow restrictor

Manjesh Kumar,Anupam Alok,Manas Das 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.11

In this article, a new flow restrictor is utilized in the magnetorheological miniature gear-profile polishing (MRMGPP) method to ensure a consistent and precise polishing of gear profiles. The effectiveness of flow restrictor is analyzed using a finite element-based software COMSOL ® Multiphysics, where shear stress and axial velocity distributions are studied along gear profiles. Later, the experiments are performed without and with using a novel flow restrictor on the SS316L miniature gear teeth profile to examine the finishing performance and results are compared with each other. After finishing the gear, the minimum surface roughness of 24.1 nm at involute profile is achieved, and also all manufacturing defects are completely removed. Concurrently, the teeth geometry profiles are not affected. The uniform finishing of miniature gear with a continuous smooth surface may improve its work performance, transmitting power ability, reliability, fatigue life and form accuracy.

Ontogeny of in vitro Shoot Organogenesis from Axillary Meristem Explants in Chickpea (Cicer arietinum L.)

Srivastava, Jaya,Das, Alok,Soren, Khela Ram,Chaturvedi, Sushil Kumar,Nadarajan, Nagaswamy,Datta, Subhojit 한국작물학회 2012 Journal of crop science and biotechnology Vol.15 No.3

Chickpea (Cicer arietinum L.) is a major food legume crops for the tropics and sub-tropical regions and is the main dietary protein source for vegetarians in developing countries. Besides several abiotic factors, its production is constrained by insect pests, as well as many fungal diseases. The success of any attempt to produce resistant varieties through genetic engineering to a large extent depends on the availability of efficient and reproducible regeneration and transformation procedures. Further, the transformation techniques can be more successfully applied if the ontogeny of shoot development is well understood. Ontogeny of shoot development from axillary meristem explants (AME) in chickpea has been studied with the optimization of conditions for high-frequency multiple shoot induction. Preculture of seeds in TDZ significantly enhances the frequency of multiple shoot induction from the explants. Ontogeny and early events of multiple shoot induction revealed direct adventitious origin of the shoots. The understanding of the regeneration process could be further utilized in the designing of efficient transformation methods.

Ontogeny of in vitro Shoot Organogenesis from Axillary Meristem Explants in Chickpea (Cicer arietinum L.)

Jaya Srivastava,Alok Das,Khela Ram Soren,Sushil Kumar Chaturvedi,Nagaswamy Nadarajan,Subhojit Datta 한국작물학회 2012 Journal of crop science and biotechnology Vol.15 No.3

Chickpea (Cicer arietinum L.) is a major food legume crops for the tropics and sub-tropical regions and is the main dietary protein source for vegetarians in developing countries. Besides several abiotic factors, its production is constrained by insect pests, as well as many fungal diseases. The success of any attempt to produce resistant varieties through genetic engineering to a large extent depends on the availability of efficient and reproducible regeneration and transformation procedures. Further, the transformation techniques can be more successfully applied if the ontogeny of shoot development is well understood. Ontogeny of shoot development from axillary meristem explants (AME) in chickpea has been studied with the optimization of conditions for high-frequency multiple shoot induction. Preculture of seeds in TDZ significantly enhances the frequency of multiple shoot induction from the explants. Ontogeny and early events of multiple shoot induction revealed direct adventitious origin of the shoots. The understanding of the regeneration process could be further utilized in the designing of efficient transformation methods. Chickpea (Cicer arietinum L.) is a major food legume crops for the tropics and sub-tropical regions and is the main dietary protein source for vegetarians in developing countries. Besides several abiotic factors, its production is constrained by insect pests, as well as many fungal diseases. The success of any attempt to produce resistant varieties through genetic engineering to a large extent depends on the availability of efficient and reproducible regeneration and transformation procedures. Further, the transformation techniques can be more successfully applied if the ontogeny of shoot development is well understood. Ontogeny of shoot development from axillary meristem explants (AME) in chickpea has been studied with the optimization of conditions for high-frequency multiple shoot induction. Preculture of seeds in TDZ significantly enhances the frequency of multiple shoot induction from the explants. Ontogeny and early events of multiple shoot induction revealed direct adventitious origin of the shoots. The understanding of the regeneration process could be further utilized in the designing of efficient transformation methods.

A Novel Additive Texturing of Stainless Steel 316 L Through Binder Jetting Additive Manufacturing

Suryank Dwivedi,Amit Rai Dixit,Alok Kumar Das,Akash Nag 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.10 No.6

The current study introduces the novel additive micro-texturing through the binder jetting additive manufacturing technique to fabricate circular micro-textured stainless steel 316L parts. The topographical analysis shows that the sample is manufactured with periodic feature sizes less than 250 μm and within an error limit of 7.62% with high metallographic purity. The wettability study indicates hydrophilic wetting behavior with contact angles in the ranges of 85.7°–82.5° for deionized water and 50.2°–36.7° for Hank’s balanced salt solution. The surface free energy for the textured part (53.484 mN/m) is improved by 72% compared to the untextured part (31.054 mN/m), leading to enhanced hydrophilicity. Further, in the scratch analysis, the textured sample shows better features intactness.

Clearance of Cervical Human Papillomavirus Infection by Topical Application of Curcumin and Curcumin Containing Polyherbal Cream: A Phase II Randomized Controlled Study

Basu, Partha,Dutta, Sankhadeep,Begum, Rakiba,Mittal, Srabani,Dutta, Paromita Das,Bharti, Alok Chandra,Panda, Chinmay Kumar,Biswas, Jaydip,Dey, Bindu,Talwar, Gursaran Prashad,Das, Bhudev Chandra Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Curcumin and curcumin containing polyherbal preparations have demonstrated anti-microbial and antiviral properties in pre-clinical studies. Till date no therapeutic intervention has been proved to be effective and safe in clearing established cervical human papillomavirus (HPV) infection. The present study evaluated the efficacy of Basant polyherbal vaginal cream (containing extracts of curcumin, reetha, amla and aloe vera) and of curcumin vaginal capsules to eliminate HPV infection from cervix. Women were screened by Pap smear and HPV DNA test by PCR. HPV positive women without high grade cervical neoplasias (N=287) were randomized to four intervention arms to be treated with vaginal Basant cream, vaginal placebo cream, curcumin vaginal capsules and placebo vaginal capsules respectively. All subjects were instructed to use one application of the assigned formulation daily for 30 consecutive days except during menstruation and recalled within seven days of the last application for repeat HPV test, cytology and colposcopy. HPV clearance rate in Basant arm (87.7%) was significantly higher than the combined placebo arms (73.3%). Curcumin caused higher rate of clearance (81.3%) than placebo though the difference was not statistically significant. Vaginal irritation and itching, mostly mild to moderate, was significantly higher after Basant application. No serious adverse events were noted.