Neutropenia during the First Cycle of Induction Chemotherapy Is Prognostic for Poor Survival in Locoregionally Advanced Nasopharyngeal Carcinoma: A Real-World Study in an Endemic Area

Cheng Xu,Shi-Ping Yang,Yuan Zhang,Ling-Long Tang,Guan-Qun Zhou,Xu Liu,Yan-Ping Mao,Rui Guo,Wen-Fei Li,Lei Chen,Ai-Hua Lin,Ying Sun,Jun Ma 대한암학회 2018 Cancer Research and Treatment Vol.50 No.3

Purpose The purpose of this study was to investigate the effect of neutropenia during the first cycle of induction chemotherapy (IC-1) on survival in locoregionally advanced nasopharyngeal carcinoma (LANPC). Materials and Methods Eligible patients (n=545) with LANPC receiving IC+concurrent chemoradiotherapy were included. Based on nadir neutrophil after IC-1, all patients were categorized into three groups: no/grade 1-2/grade 3-4 neutropenia. Five-year overall survival (OS) and disease-free survival (DFS) were compared between groups and subgroups stratified by IC regimen. We also explored the occurrence of IC-1–induced myelosuppression events and the minimal value of post-treatment neutrophil-to-lymphocyte ratio (post-NLRmin). Univariate/multivariate analyses were performed to investigate the effect of IC-1–induced neutropenia, timing of neutropenia, number of myelosuppression events, and high post-NLRmin on OS/DFS. Results Grade 1-2/grade 3-4 neutropenia were associated with poorer OS/DFS than no neutropenia (all p < 0.05); OS/DFS were not significantly different between patients experiencing grade 1-2 vs. 3-4 neutropenia. Neutropenia had no significant effect on OS/DFS in patients receiving docetaxel–cisplatin–5-fluorouracil (TPF). Grade 1-2 (grade 3-4) neutropenia negatively influenced OS/DFS in patients receiving cisplatin–5-fluorouracil (PF) (PF and docetaxel– cisplatin [TP]; all p < 0.05). Neutropenia, two/three myelosuppression events, and high post-NLRmin ( 1.33) was most frequent on days 5-10, second and third week of IC-1, respectively. After adjustment for covariates, IC-1–induced neutropenia, two/three myelosuppression events, and post-NLRmin  1.33 were validated as negative predictors of OS/DFS (all p < 0.05); timing of neutropenia had no significant effect. Conclusion Occurrence of neutropenia, number of myelosuppression events, and high post-NLRmin during PF/TP IC-1 have prognostic value for poor survival in LANPC.

Selection of High Laccase-Producing Coriolopsis gallica Strain T906: Mutation Breeding, Strain Characterization, and Features of the Extracellular Laccases

( Xiaoli Xu ),( Lei Feng ),( Zhenya Han ),( Sishi Luo ),( Ai`min Wu ),( Jun Xie ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.9

Commercial application of laccase is often hampered by insufficient enzyme stocks, with very low yields obtained from natural sources. This study aimed to improve laccase production by mutation of a Coriolopsis gallica strain and to determine the biological properties of the mutant. The high-yield laccase strain C. gallica TCK was treated with N-methyl-N-nitro Nnitrosoguanidine and ultraviolet light. Among the mutants isolated, T906 was found to be a high-production strain of laccases. The mutant strain T906 was stabilized via dozens of passages, and the selected ones were further processed for optimization of metallic ion, inducers, and nutritional requirements, which resulted in the optimized liquid fermentation medium MF9. The incubation temperature and pH were optimized to be 30°C and 4.5, respectively. The mutant strain T906 showed 3-times higher laccase activity than the original strain TCK under optimized conditions, and the maximum laccase production (303 U/ml) was accomplished after 13 days. The extracellular laccase isoenzyme 1 was purified and characterized from the two strains, respectively, and their cDNA sequence was determined. Of note, the laccase isoenzyme 1 transcription levels were overtly increased in T906 mycelia compared with values obtained for strain TCK. These findings provide a basis for C. gallica modification for the production of high laccase amounts.

Microwave-assisted pyrolysis of phosphoric acid-activated Goldenberry peel powder biochar for enhancing the adsorption of trace beta-lactamase inhibitors

Tian Ai,Chunmei Xu,Lei Zhang,Ke Chen,Yonggui Wu,Shujuan Dai,Xiaolu Xiong,Shixin Jie,Xiaoni Jin,Zhongxu Yu 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.9

Novel efficient biochar of Goldenberry peels (GBPMW-H3PO4) was prepared through a microwave-assistedphosphoric acid activation method. It was characterized and used for removing two beta-lactamase inhibitors, sulbactam(SAM, first listed in Japan in 1986) and avibactam (AVI, first listed in the U.S. in 2015), from aqueous solution. Characterization confirmed that GBPMW-H3PO4 displayed a high surface area (720.046m2 g1), more abundant porestructure, smaller particle size, and higher thermal stability. The experimental results showed that the adsorption of thetwo antibiotics was a spontaneous, favorable, and endothermic process, highly dependent on solution pH. A contacttime of 60 min assured equilibrium, and GBPMW-H3PO4 followed pseudo-first-order kinetics (R2=0.9950-0.9977). Furthermore,the adsorption capacities of GBPMW-H3PO4 for SAM and AVI were 211.86 and 198.81mg g1, respectively,and the performance was better than that of unmodified biochar. Microscopically, the main mechanism could beexplained by - electron donor-acceptor interaction, hydrogen bonding interaction, -hydrogen bonding, hydrophobicinteraction, and electrostatic interaction. The study demonstrates that the microwave-assisted H3PO4 activationmethod could produce biochar, and GBPMW-H3PO4 was confirmed to be a low-cost and high-efficiency adsorbent forremoving beta-lactamase inhibitors from medical wastewater.

Comparison of Fungal Community Structure between Vegetable Soil and Rice Paddy Soil

Juan-Li Lei,Zhi-Hao Xu,Wei-Song Shou,Wen-Qi Dong,Ai-fang Wu,Cheng-Hao Zhang,Hyung Kweon Yun 한국원예학회 2008 한국원예학회 기타간행물 Vol.- No.-

Combination of Probiotics and Salvia miltiorrhiza Polysaccharide Alleviates Hepatic Steatosis via Gut Microbiota Modulation and Insulin Resistance Improvement in High Fat-Induced NAFLD Mice

Wei Wang,Ai-Lei Xu,Zheng-Chao Li,Yi Li,Shun-Fu Xu,Hua-Chao Sang,Fachao Zhi 대한당뇨병학회 2020 Diabetes and Metabolism Journal Vol.44 No.2

Background: Nonalcoholic fatty liver disease (NAFLD) increases the risk of hepatocellular carcinoma, which is currently the leading cause of obesity-related cancer deaths in middle-aged men. Methods: Probiotics with lipid-lowering function were screened from the fecal microbiota of healthy adults. Polysaccharide from different sources was screened for improving insulin resistance. The combination of probiotics and Salvia miltiorrhiza polysaccharide (LBM) was investigated for alleviating hepatic steatosis. Results: First, Bifidobacterium bifidum V (BbV) and Lactobacillus plantarum X (LpX) were obtained from the fecal microbiota of healthy adults. Second, to improve insulin resistance, a Salvia miltiorrhiza Bunge polysaccharide showing good performance in reducing insulin resistance was obtained. The liver total cholesterol (TC) and total triglyceride (TG) levels and the serum levels of free fatty acid, alanine transaminase, aspartate transaminase, low density lipoprotein cholesterol, TG, and TC can be significantly reduced through supplementation with LpX-BbV (LB) in NAFLD mice. Interestingly, the function of the probiotic LB can be enhanced by S. miltiorrhiza Bunge polysaccharide. Furthermore, the gut microbiota was modulated by LpX-BbV+S. miltiorrhiza Bunge polysaccharide (LBM). The lipopolysaccharide concentration of the LBM group was decreased by 73.6% compared to the NAFLD group. Ultimately, the mRNA concentrations of the proinflammatory cytokines (tumor necrosis factor α, interleukin 1β [IL-1β], and IL-6) decreased with LB and LBM treatment. Conclusion: The results of this this study indicate that the LBM combination can be used as a therapeutic for ameliorating NAFLD via modulating the gut microbiota and improving insulin resistance.

Stable Cu-Co/C carbon-based composites for efficiency catalytic degradation of Orange II by PMS: Effect factors, application potential analysis, and mechanism

Xiaodan Huang,Caihua Liu,Zhiyong Zhang,V. Vasanthakumar,Huiying Ai,Lei Xu,Ming-Lai Fu,Baoling Yuan 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.126 No.-

Carbon-based metallic catalysts (CMCs) have emerged as efficient materials for removing water contaminants,but preparing CMCs with high efficiency and stability remains a great challenge. In this study, weprepared a bimetallic composite of Cu-Co/C catalyst via a gelation and calcination method to degradeOrange II by activating peroxymonosulfate (PMS). The characterization results demonstrated that thespecific surface area and pore size of Cu-Co/C catalysts were 1.675 m2/g and 6.313 nm, respectively,which can provide larger catalytic active sites for boosting PMS activation. Moreover, the effects of differentfactors on Orange II degradation efficiency, cyclic experiments, and possible application in realwater bodies were investigated. The Cu-Co/C catalyst can effectively activate PMS, and 50 mg/L ofOrange II was almost completely removed within 15 min. Additionally, the Cu-Co/C catalyst exhibitedhigh degradation efficiency for other organic dye pollutants. The radical quenching experiments indicatedthat the main active species for Cu-Co/C catalyzed degradation of Orange II were SO4and singlet oxygen(1O2). The catalyst demonstrated good stability and performance in real water bodies. We believe thatthis study will promote the application of carbon-based bimetallic catalysts in the environmental remediationprocess that utilize SO4based advanced oxidation.