Early Phase of UVB - induced GM - CSF Upregulation in Epithelial Cell Line is not Totally Dependent on IL - 1α

Park, Kyoung Chan,Kim, Kyu Han,Ahn, Jong Seong,Chung, Jin Ho,Youn, Jai Il,Whang, Ji Hwan,Youn, Sang Woong,Kim, Young Gull,Koh, Woo Seok,Jung, Hyun Chae 대한피부과학회 1997 Annals of Dermatology Vol.9 No.4

Backgrounds : It was demonstrated that ultraviolet(UV) B light induces the release of IL-la in cultured human epithelial cell line and augmentation of GM-CSF production by UVB is reported to be mediated by IL-1α in the murine keratinocyte cell line Pam 212. Objective : The purpose of this study was to evaluate the effects of UVB on kinetic profile of IL-1 and GM-CSF mRNA expression and to see whether synthesis of GM-CSF by UVB can be completely inhibited by blocking IL-1α mediated pathway. Method : We used a competitive RT-PCR for measuring cytokine gene expression in epithelial cell line after UV radiation. Results : The IL-1α mRNA increased as early as 1h after UV irradiation, and then decreased at 3h after the irradiation. Thereafter, the response of IL-1α mRNA was upregulated with a second peak at 6h after the UV irradiation. However, mRNA for GM-CSF increased at 1h after UV light exposure and anti-IL-1α antibodies could only partially inhibit UV-augmented GMCSF production. Conclusion : UVB induced GM-CSF production seemed to be mainly mediated by UVB induced IL-1α but these results suggest that UVB may also induce GM-CSF production through an IL-1α independent pathway.

GATA-3 is a Key Factor for Th1/Th2 Balance Regulation by Myristicin in a Murine Model of Asthma

이규,이창민,정인덕,정영일,천성학,박희주,최일환,안순철,신용규,이상율,염석란,김종석,박영민,Lee, Kyu,Lee, Chang-Min,Jung, In-Duk,Jeong, Young-Il,Chun, Sung-Hak,Park, Hee-Ju,Choi, Il-Whan,Ahn, Soon-Cheol,Shin, Yong-Kyoo,Lee, Sang-Yull,Yeom, S Korean Society of Life Science 2007 생명과학회지 Vol.17 No.8

Myristicin은 육두구에서 발견되는 고농축 정유 중 하나인 물질이다. 하지만 Th1/Th2 면역반응에서 육두구의 항알레르기 효과는 아직 밝혀지지 않았다. 최근에 Th1/Th2 전사인자로서 T-bet, GATA-3가 밝혀졌는데 이번 실험에서 myristicin이 ovalbumin(OVA)으로 유도한 천식(asthma) 생쥐모델에서 Th1,Th2 싸이토카인과 유전자 발현을 조절할 수 있는가에 대하여 알아보았다. 또한 기관지 폐포 세척액을 회수하여 백혈구의 수적 변화, 제2형 협조T세포(Th2 cell)가 생산하는 IL-4, IL-5의 생산에 미치는 영향과 폐조직에서 matrix metalloproteinase (MMP)-9 활성을 측정하였다. 그 결과 기관지 폐포 세척액에서 OVA로 감작하여 천식을 유도한 실험군에서는 호산구의 현저한 증가, Th2 형 싸이토카인(IL-4, IL-5)의 증가가 관찰되었다. 그러나 myristicin을 투여한 그룹에서는 OVA의 감작에 의하여 증가한 각종 염증성 지표들이 감소하거나 정상화 되었다. 또한 OVA에 의하여 증가된 기도저항성이 myristicin 투여에 의하여 감소하였으며 폐조직의 염증성 소견도 뚜렷하게 감소되었다. 이와 같은 연구 결과는 myristicin이 천식의 치료에 유용하게 쓰일 수 있음을 시사해준다. Myristicin, l-allyl-3,4-methylenedioxy-5-methoxybenzene, was one of the major essential oils of nutmeg. However, its anti-allergic effect in the Th1/Th2 immune response was poorly understood. Recently, it was shown that T-bet and GATA-3 was master Th1 and Th2 regulatory transcription factors. In this study, we have attempted to determine whether myristicin regulates Th1/Th2 cytokine production, T-bet and GATA-3 gene expression in ovalbumin (OVA)-induced asthma model mice. Myristicin reduced levels of IL-4, Th2 cytokine production in OVA-sensitized and challenged mice. In the other side, it increased $IFN-{\gamma}$, Th1 cytokine production in myristicin administrated mice. We also examined to ascertain whether myristicin could influence eosinophil peroxidase (EPO) activity. After being sensitized and challenged with ovalbumin (OVA) showed typical asthmatic reactions. These reactions included an increase in the number of eosinophils in bronchoalveolar lavage fluid, an increase in inflammatory cell infiltration into the lung tissue around blood vessels and airways, and the development of airway hyper-responsiveness (AHR). The administration of myristicin before the last airway OVA challenge resulted in a significant inhibition of all asthmatic reactions. Accordingly, these findings provide new insight into the immunopharmacological role of myristicin in terms of its effects in a murine model of asthma.

청력 보호구 착용에 대한 점검이 보호구 착용율에 미치는 영향

박상일,이용진,김화성,안규동,이병국 순천향대학교 산업의학연구소 2001 순천향산업의학 Vol.7 No.1

This study was carried out to investigate the effects on wearing hearing protector by checking hearing protector. Research subjects were 333 workers in 5 fiber industries and which were noise exposed above 90dB(A). The frequency of checking and wearing rate of hearing protector by sex, age, work duration and grade of health administration personnel were obtained by self-administered questionnaire and check list. The results were as followed. 1. The wearing rates of hearing protector by checking hearing protector were 50.2%, 51.4%, 61.3%, and 64.3%, respectively and those were significant increasing trends(p<0.05). 2. The wearing rate of hearing protector was significantly increased with increasing frequency of checking hearing protector regardless of sex(p<0.O5). 3. The wearing rate of hearing protector was increased with increasing frequency of checking hearing protector regardless of age. Aged above 20 years or below 50 years were significantly increased(p<0.O5), whereas aged below 20 years and above 50 years were not significantly increased. 4. The wearing rate of hearing protector was increased with increasing frequency of checking hearing protector regardless of work duration. Work durations below 10 years were significantly increased (p<0.O5), whereas work durations above 10 years were not significantly increased. 5. The wearing rate of hearing protector was increased with increasing frequency of checking hearing protector regardless of grade of health administration personnel. Grade above chief was significantly increased(p<0.05), whereas grade below chief was not significantly increased. As the result of this study, it was suggested that the wearing rate of hearing protector was related to frequency of checking hearing protector. The continuous check up of hearing protector was recommended for the effective health management.

비호지킨 림프종환자에서 proMACE-cytaBOM 복합항암화학요법의 유효성에 대한 제 2상 임상시험

김성우,안명주,박석규,이영렬,정태준,최일영,김인순 한양대학교 의과대학 2000 한양의대 학술지 Vol.20 No.1

Aggressive non-Hodgkin's lymphoma by REAL classification is a heterogenous group of disease. With recent advances in combination chemotherapy, the long-term survival is improved to 40-65%. We investigated twenty-one aggressive non-Hodgkin's lymphoma patients treated with pro-MACE-cytaBOM (prednisone, adriamycin, cyclophosphamide, etoposide, cytosine arabinoside, bleomycin, vincristine and methotrexated) to determine the efficacy and toxicites. There were seven patients older than 60 years. Histologic types by REAL classification were as follows : diffuse large B cell (12/21), peripheral T cell (6/21), anaplastic large cell (2/21) and angiocentric T cell (1/21). Most of patients were classified in Stage Ⅱ-Ⅳ (20/21). According to IPI (international prognostic index) score, seven patients were included in low risk, five were low-intermediate risk, three were high-intermediated risk and six were high risk group. seventeen complete responses (85%) and two partial responses (10%) were achieved. The overall response rate was 95%. The mena disease free survival was 30 months, the mean overall survival was 29 months (range:21-36), and the median overall survival was not reached. WHO grade Ⅲ/Ⅳ neutropenia or thrombocytopenia was observed in 16.7% and 2.8%, respectively. There was no treatment-related death. These results suggest that proMACE-cytaBOM combination chemotherapy is very effective and has low toxicity in the treatment of aggressive non-Hodgkin's lymphoma even treated in elderly patients.

Suppression of IgE production and modulation of Th1/Th2 cell response by electroacupuncture in DNP-KLH immunized mice

Park, Moon-Baik,Ko, Eunjung,Ahn, Changjoon,Choi, Hyun,Rho, Samwoong,Shin, Min-Kyu,Hong, Moo-Chang,Min, Byung-Il,Bae, Hyunsu WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2004 東西醫學硏究所 論文集 Vol.2004 No.-

Effects of electroacupuncturc (EA) on Th1/Th2 cell response were investigated in BALB/c mice immunized intraperitoneally with 2,4-dinitrophenylated keyhole limpet protein (DNP-KLH). Successive electroacupuncture stimulation on the ST36 acupoint was performed just after immunization. Serum levels of antigen-specific lgE and total lgE were significantly decreased compared with non-acupunctured controls. Production of the Th2-specific cytokines IL-4 and IL-13 in the anti-CD3 mAb-activated splenocytes was significantly suppressed in ST36 electroacupunctured mice compared with non-acupunctured mice. These results imply that successive electroacupuncture on ST36 can decrease the serum level of antigen-specific lgE and total lgE by suppression of the Th2 lineage development.

Comparison of Foot Pressure Distribution During Single-leg Squat in Individuals With and Without Pronated Foot

Ahn Il-kyu,Gwak Gyeong-tae,Hwang Ui-jae,Yoo Hwa-ik,Kwon Oh-yun 한국전문물리치료학회 2024 한국전문물리치료학회지 Vol.31 No.1

Background: Single-leg squat (SLS)s are commonly used as assessment tool and closed kinetic exercises are useful for assessing performance of the lower extremities. Pronated feet are associated with foot pressure distribution (FPD) during daily activities. Objects: To compare the FPD during SLSs between groups with pronated and normal feet. Methods: This cross-sectional study included 30 participants (15 each in the pronated foot and control groups) are recruited in this study. The foot posture index was used to distinguish between the pronated foot and control groups. The Zebris FDM (Zebris Medical GmbH) stance analysis system was used to measure the FPD on the dominant side during a SLS, which was divided into three phases. A two-way mixed-model ANOVA was used to identify significant differences in FPD between and within the two groups. Results: In the hallux, the results of the two-way mixed-model ANOVAs revealed a significant difference between the group and across different phases (p < 0.05). The hallux, and central forefoot were significantly different between the group (p < 0.05). Moreover, significant differences across different phases were observed in the hallux, medial forefoot, central forefoot, lateral forefoot, and rearfoot (p < 0.05). The post hoc t-tests were conducted for the hallux and forefoot central regions. In participants with pronated foot, the mean pressure was significantly greater in hallux and significantly lower, in the central forefoot during the descent and holding phases. Conclusion: SLSs are widely used as screening tests and exercises. These findings suggest that individuals with pronated feet should be cautious to avoid excessive pressure on the hallux during the descent-to-hold phase of a SLS.

전립선 비대증 환자에서 국제 전립선 증상 점수에 영향을 주는 요소에 관한 연구

안규일(Kyu Il Ahn),김영주(Young-Ju Kim),장성구(Sung-Goo Chang) 대한비뇨기종양학회 2003 대한비뇨기종양학회지 Vol.1 No.3

국소 전립선암 환자에서 근치적 치료 후 생화학적 재발 및 임상적 재발에 관한 연구

안규일(Kyu IL Ahn),장성구(Sung-Goo Chang),최봉근(Bong-Keun Choi),전승현(Seung Hyun Jeon) 대한비뇨기종양학회 2004 대한비뇨기종양학회지 Vol.2 No.3

Progeny Selection and Molecular Characterization for Transgenic PAC Soybean

Yang Qin,Hong-Il Ahn,Bum-Kyu Lee,Young-Soo Jeong,Jong-Min Ko,Soon-Ki Park,Soon-Jong Kweon 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Two carotenoid biosynthetic genes, phytoene synthase (Psy) and carotene desaturase (CrtI) linked via synthetic 2A sequence under control of CaMV 35S promoter (two T0 plants 5 and 6) or β- conglycinin promoter (three T0 plants 7, 13 and 16) were transformed into soybean variety Kwangan. After agronomic and phenotypic selection at early generations, T5 progeny of PAC soybean were analyzed by Southern blot to confirm T-DNA copy numbers. A total of 27 homologous lines derived from one of three T0 plants (line 7 under the control of β- conglycinin promoter) with one copy T-DNA insertion, were separated and planted into greenhouse. Flanking sequence analysis was carried out on one of homologous line 6-2-3 and results indicated the T-DNA was intergenic inserted into chromosome 14 from 10,873,131 to 10,872,998 base of soybean chromosome. T-DNA insertion structure, flanking sequence and inserted gene expressions need to be analyzed in the further study.

A Novel Hyaluronate-Atelocollagen/β-TCP-Hydroxyapatite Biphasic Scaffold for the Repair of Osteochondral Defects in Rabbits

Ahn, Ji-Hyun,Lee, Tae-Hyeong,Oh, Jong-Soo,Kim, Su-Yeon,Kim, Hyun-Jung,Park, Il-Kyu,Choi, Baek-Sun,Im, Gun-Ii Mary Ann Liebert 2009 Tissue engineering. Part A Vol.15 No.9

<P>The authors devised a novel biphasic scaffold combining hyaluronic acid and atelocollagen for the chondral phase and combining hydroxyapatite (HA) and beta-tricalcium phosphate (beta-TCP) for the osseous phase. The biphasic scaffold was fabricated by placing the freeze-dried chondral phase over the HA/beta-TCP scaffold prewetted with hyaluronate/atelocollagen solution. Chondrocytes were isolated in 28 rabbits, expanded, injected inside the chondral phase of the biphasic scaffold, and then cultured in chondrogenic medium. After 2 weeks of in vitro culture, chondrocytes had evenly infiltrated inside the chondral phase and produced extracellular matrix. For in vivo study, a large osteochondral defect was made on the patellar groove of the right distal femur and managed using one of the following methods: filling with cell-biphasic scaffold composite (group I); implanting only biphasic scaffold (group II); placing the removed osteochondral fragments back into the defect (group III, positive control); leaving empty (group IV, negative control). Seven rabbits were allocated to each group. After 12 weeks, the International Cartilage Repair Society Macroscopic Score was highest in group III, followed by group I, group II, and lastly group IV. Depression of the defect was greatest in group IV. There were three rabbits (two in group I and one in group II) that were completely denuded of the chondral phase. The junction to adjacent native cartilage was distinct in rabbits of all groups. The International Cartilage Repair Society Visual Histological Score was highest in group III, followed by groups II and I, and lastly group IV. In conclusion, our results suggest that a biphasic osteochondral composite using a chondral phase consisting of hyaluronate and atelocollagen and an osseous phase consisting of HA and beta-TCP holds the promise for repair of osteochondral defects.</P>