Advanced H2O2 oxidation for diethyl phthalate degradation in treated effluents: effect of nitrate on oxidation and a pilot-scale AOP operation

Ko, K. B.,Park, C. G.,Moon, T. H.,Ahn, Y. H.,Lee, J. K.,Ahn, K. H.,Park, J. H.,Yeom, I. T. IWA Publishing 2008 Water Science & Technology Vol.58 No.5

<P>One of the objectives of this study was to delineate the effect of nitrate on diethyl phthalate (DEP) oxidation by conducting a bench-scale ultraviolet (UV)/H2O2 and O3/H2O2 operations as suggested in a previous study. We also aim to investigate DEP oxidation at various UV doses and H2O2 concentrations by performing a pilot-scale advanced oxidation processes (AOP) system, into which a portion of the effluent from a pilot-scale membrane bioreactor (MBR) plant was pumped. In the bench-scale AOP operation, the O3 oxidation alone as well as the UV irradiation without H2O2 addition could be among the desirable alternatives for the efficient removal of DEP dissolved in aqueous solutions at a low DEP concentration range of 85±15 μg/L. The adverse effect in the UV/H2O2 process was significantly greater than that in the UV oxidation alone, and its oxidation was almost halved by the nitrate. However, the nitrate clearly enhanced the DEP oxidation in the O3 oxidation and O3/H2O2 process. Especially, the addition of nitrate almost doubled the DEP oxidation efficiency in the O3/H2O2 process. The series of pilot-scale AOP operations confirmed that about 30-50% of DEP dissolved in the treated MBR effluent streams was, at least, oxidized by the O3 oxidation alone as well as the UV irradiation without H2O2 addition. The UV photolysis of H2O2 was most effective for DEP degradation with an H2O2 concentration of 40 mg/L at a UV dose of 500 mJ/cm2.</P>

Effects of Egg Yolk Antibodies Produced in Response to Different Antigenic Fractions of E. coli O157:H7 on E. coli Suppression

Chae, H.S.,Singh, N.K.,Ahn, C.N.,Yoo, Y.M.,Jeong, S.G.,Ham, J.S.,Kim, D.W. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.11

The objective of this research was to provide the characterization and method for producing anti-E. coli O157:H7 antibodies in egg-laying hens and to determine if the antibody can restrain the proliferation of E. coli O157:H7 in-vitro. Selected antigenic fractions (whole cell, outer membrane protein and lipopolysaccharide (LPS)) from E. coli O157:H7 were injected to hens in order to produce anti-E. coli O157:H7 antibodies. The immune response and the egg yolk antibodies of laying hens against the whole cell, outer membrane protein and LPS antigens were monitored by ELISA. The level of antibodies against whole cell antigen monitored through ELISA sharply increased after the initial immunization, and it was found to be maximum on day 49 however, the level was maintained up to day 70. Antibodies (5 mg/ml) directed against the whole cell inhibited E. coli proliferation 10-13 times more than outer membrane protein or LPS. The antibody response against the whole cell antigens appeared to have higher activity in restraining the proliferation of E. coli O157:H7 than antibody against outer membrane protein or LPS. Results reflected that increasing the IgY's in the egg yolk could prevent greater economic losses due to human and animal health from pathogenic bacteria i.e. E. coli O157:H7.

Phase II study of preoperative chemoradiotherapy (CRT) with irinotecan plus S-1 in locally advanced rectal cancer

Shin, S.J.,Kim, N.K.,Keum, K.C.,Kim, H.G.,Im, J.S.,Choi, H.J.,Baik, S.H.,Choen, J.H.,Jeung, H.C.,Rha, S.Y.,Roh, J.K.,Chung, H.C.,Ahn, J.B. Elsevier Science Publishers 2010 Radiotherapy and oncology Vol.95 No.3

Background and purpose: The aim of this study is to evaluate the efficacy and safety of preoperative radiation therapy combined with S-1 and irinotecan (SI) in LARC. Materials and methods: Patients were considered LARC if they had a T3/T4 lesion or node positive. Weekly doses of 40mg/m<SUP>2</SUP> irinotecan were intravenously administered once per week during weeks 1-5 of radiotherapy. S-1 (70mg/m<SUP>2</SUP>) was given from Monday to Friday in all weeks of radiotherapy. 3-D conformal radiotherapy was given at daily fractions of 1.8Gy for 5days for a total dose of 50.4 (45+5.4)Gy. Surgery was performed 4-6weeks following the completion of chemoradiation. Results: Between June 2006 and November 2007, 43 pts were enrolled. The stage was: cT3 24 patients, cT4 6 patients; 28 patients were cN+. Forty-one patients completed the chemoradiation and 42 patients underwent operation: a low anterior resection was performed in 36 patients, a total colectomy in 1 patient, and an abdominal perineal resection in 5 patients. T downstaging was observed in 50%; 23 N+ patients became N- (55%). The complete pathological response was observed in 9 patients (21%). The 3-year locoregional failure rate, distant failure rate, disease-free survival, and overall survival were 9.5%, 18.6%, 72.1%, and 94.3%, respectively. Only three patients experienced G3 diarrhea; one had G3 sepsis and two had septic shock. Hematological toxicity (G3-G4) was observed in five patients. Conclusions: This study demonstrated the efficacy of preoperative CRT with S-1 and irinotecan with 21% of complete response. However, prompt recognition and management of infection is needed to use it in patients with locally advanced rectal cancer.

한국 재래 닭 부화 후 고환 발달에 관한 연구

장병귀,태현진,최철환,박영재,박병용,박상열,강형섭,김남수,이영훈,양홍현,안동춘,김인식,Jang, B.G.,Tae, H.J.,Choi, C.H.,Park, Y.J.,Park, B.Y.,Park, S.Y.,Kang, H.S.,Kim, N.S.,Lee, Y.H.,Yang, H.H.,Ahn, D.C.,Kim, I.S. 한국가금학회 2006 韓國家禽學會誌 Vol.33 No.3

이 연구는 한국 재래 닭에서 부화 후 고환 발달 과정을 명확하게 이해가기 위하여 부화 후 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 21, 28, 32, 44, 52 및 64주령(n=13마리/일령)의 한국 재래닭을 이용하여 수행하였다. 한국 재래 닭의 고환은 2.5 % glutaraldehyde를 이용하여 전신 관류 고정하고 조직 처리 과정을 거쳐 Epon-araldite에 포매하였다. 초박절편기를 사용하여 $1{\mu}m$로 절편한 다음 methylene blue로 염색하여 일반적인 조직의 변화상과 형태 계측을 일반적인 방법에 따라서 정자 생성을 측정하여 다음과 같은 결과를 얻었다. 부화 후 1주령의 고환의 평균 무게는 0.015 g이었고 점진적으로 증가하여 21주령에는 3.93 g이고 21주령부터 64주령까지는 변화가 없었다. 곱슬정세관의 용적 치밀도는 1주령에 32.6%이었으나 점차적으로 증가하여 64주령에서는 92.89이었다. 1주령의 한국 재래 닭 고환 간질 조직은 고환 실질의 67.4%를 나타내었고 이러한 비율은 성장하는 동안에 점차적으로 감소하여 64주령에 7.11%를 나타내었다. 고환내 총 정자 생성은 18주령부터 28주령까지는 유의성있게 증가하였고 고환 1g당 정자 생성은 $18\sim28$주령까지는 유의성있게 증가하였고 $28\sim52$주령까지는 변화가 없었으나 64주령에 유의성 있게 감소하였다. 곱슬정세관의 평균 직경은 $1\sim21$주령까지 주령에 따라 점진적으로 증가하였고 곱슬정세관의 길이는 1주령에 0.34 m이었고 성장하면서 유의성 있게 증가하여 64주령에서는 72.2 m이었다. 곱슬정세관내 생식세포의 발달 단계는 다음과 같이 분류할 수 있다. 1) 정조세포($1\sim8$주령), 2) 정조세포, 정모세포($10\sim12$주령), 3) 정조세포, 정모세포, 원형의 정자세포($14\sim16$주령), 4) 정조세포, 정모세포, 정자세포 및 정자($18\sim64$주령). 이러한 결과를 종합하여 보면 한국 재래 닭에서 부화 후부터 성숙시기까지 고환 발달의 양상은 신생시기-성 성숙 이전기($1\sim12$주령), 성 성숙시기($14\sim18$주령) 및 성숙시기$(21\sim64)$로 나뉜다. Changes in the chicken testis from hatching to adulthood were studied in Korean native chickens of 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 21, 24, 28, 32, 44, 52 and 64 weeks (n=13 chickens per group) of age. The present study was to investigate in more detail the post-hatching development of testis in Korean native chickens. Testes of chickens were fixed by whole body perfusion using a fixative containing 2.5% glutaraldehyde in cacodylate buffer, processed and embedded in Epon-araldite. Using $1{\mu}m$ sections stained with methylene blue-azure II, qualitative and quantitative(stereological) morphological studies were performed. Sperm production was measured by routine technique. The average volume of a testis of 1 week old Korean native chickens was determined as 0.015 g and the parameter increased linearly from 1 week to 21 weeks days (28.9 g), and did not change from 21 weeks to 64 weeks. The volume density of the seminiferous tubules increased with age from 32.6% at week 1 to 92.89% at week 64. The volume density of the interstitium represents 67.4% of the testicular parenchyma at week 1. This proportion progressively diminished during development to reach a value of 7.11% at week 64. Total sperm production per testis increased significantly from 18 weeks to 28 weeks and remained unchanged. Sperm production per 1 g testis increased significantly from 18 weeks to 28 weeks, did not change significantly from 28 weeks to 52 weeks, and declined significantly at 64 weeks of age. The average diameter of the seminiferous tubules gradually increased with age from 1 week $(42.4{\mu}m)$ to 21 weeks $(412.8{\mu}m)$. The length of the seminiferous tubules was 0.34 m at 1 week, increased significantly in subsequent age groups and reached 72.2 m by weeks 64. The stage of germ cell development in seminiferous tubules was classified as 1) spermatogonia $(1\sim8\;weeks)$, 2) spermatogonia and spermatocytes $(10\sim12\;weeks)$, 3) spermatogonia, spermatocytes and round spermatids $(14\sim16\;weeks)$, and 4) speramatogonia, spermatocytes, spermatids and spermatozoa $(18\sim64\;weeks)$. These results clarified the pattern of changes in the testicular development in Korean native chickens from hatching to adulthood as 1) neonatal-prepubertal $(1\sim12\;weeks)$, 2) puberty$(14\sim18\;weeks)$, and adult$(21\sim64\;weeks)$.

HF:H₂O₂:CH₃COOH 용액을 이용한 실리콘-져마늄 에피막의 화학적 선택 식각

안창근,정욱진,배영호,김광일,강봉구,권영규,손병기 경북대학교 센서기술연구소 1995 센서技術學術大會論文集 Vol.6 No.1

The selective chemical etching of Si_(1)-_(x)Ge_(x). heteroepitaxial layer grown by rapid thermal CVD (RTCVD) method has been studied with respect to Ge content ( 0.15 ≤ x ≤ 0.30 ). The properties of HF : H_(2)O_(2) : CH_(3)COOH (1:2:3) chemical etchant is highly selective in the Si_(1)-_(x)Ge_(x) / Si heteroepitaxial structure. The selectivity is presented better than 100 for Ge content (x≥0.20) and increased with Ge content in Si_(1)-_(x)Ge_(x) heteroepitaxial layer.

5-Nitro-5'-hydroxy-indirubin-3'-oxime (AGM130), an indirubin-3'-oxime derivative, inhibits tumor growth by inducing apoptosis against non-small cell lung cancer in vitro and in vivo

Ahn, M.Y.,Kim, T.H.,Kwon, S.M.,Yoon, H.E.,Kim, H.S.,Kim, J.I.,Kim, Y.C.,Kang, K.W.,Ahn, S.G.,Yoon, J.H. Elsevier 2015 European journal of pharmaceutical sciences Vol.79 No.-

This study examined the anti-tumor effects of AGM130, a novel indirubin-3'-oxime derivative in A549 human non-small cell lung cancer cells. AGM130 significantly inhibited the proliferation and arrested the cell cycle of G2/M phase. Induction of apoptosis was detected in AGM130-treated A549 cells. The protein levels of Cytochrome c release, Bax, cleaved caspases and PARP were increased in AGM130 treated cells, whereas Bcl-2 levels were decreased. AGM130 inhibited Insulin-like growth factor 1 receptor (IGF1R), AKT/mTOR signaling and inactivated mitogen-activated protein kinases (MAPK). AGM130 also induced slight autophagy as pro-survival function and autophagy inhibition by chloroquine (CQ) induced necrosis. In vivo tumor xenograft model, AGM130 dose-dependently suppressed transplanted A549 cell tumor growth and induced the expression of proliferative cell nuclear antigen (PCNA). AGM130 also increased TUNEL positive apoptotic cell populations and the induction of glandular differentiation with mucin pool compared with vehicle-treated control in tumor tissue. These results suggest that AGM130 is an effective novel indirubin-3'-oxime derivative of anti-cancer drug and may be an attractive candidate for non-small cell lung cancer therapy.

Inducible Expression of Plasma Membrane H⁺-ATPase in the Roots of Figleaf Gourd Plants under Chilling Root Temperature

Ahn S. J(안성주),Im Y. J.(임양주),Chung G. C.(정갑채),Cho B. H.(조백호) 한국원예학회 1998 원예과학기술지 Vol.16 No.3

Topical delivery of 4-hexylresorcinol promotes wound healing via tumor necrosis factor-α suppression

Ahn, J.,Kim, S.G.,Kim, M.K.,Kim, D.W.,Lee, J.H.,Seok, H.,Choi, J.Y. Butterworths ; Elsevier 2016 BURNS -OXFORD- Vol.42 No.7

<P>Objective: The objective of this study was to evaluate (1) the effect of 4-hexylresorcinol (4HR) application on TNF-alpha expression in RAW264.7 cells and (2) the effect of 4HR ointment on burn wound healing in a rat burn wound model. Materials and methods: RAW264.7 cells were cultured in the presence of different concentrations of 4HR, and the attached cells were sampled for western blot analysis at each time point. Animal studies were conducted on male Wistar rats (n = 24). Three treatment groups were evaluated in this study: (1) treatment with ointment alone (negative control), (2) treatment with ointment containing 0.2 wt% 4HR, and (3) treatment with ointment containing 2 wt% 4HR. For the assessment of wound healing, digital photographs were taken at 1, 5, and 14 days. Animals were sacrificed at 1, 5, and 14 days for histological examination or ELISA analysis. Results: Western blot analysis showed that TNF-alpha protein levels were reduced following treatment with 4HR at concentrations between 1 and 10 mu g/mL. In animal studies, the average denuded area 14 days after burn was smaller in the 2 wt% 4HR treatment group compared with the control group (P = 0.022). Immunohistochemical analysis revealed lower TNF-alpha expression in the 2 wt% 4HR treatment group compared with the control group 14 days after burn (P = 0.027). Conclusion: 4HR reduced TNF-alpha expression in RAW264.7 cells and in the burn wounds of rats. Burn wounds dressed with ointment containing 4HR also exhibited rapid epithelization and collagen regeneration. (C) 2016 Elsevier Ltd and ISBI. All rights reserved.</P>

EFFECTS OF THE REMOTE H-PLASMA TREATMENTS FOR THE REMOVAL OF CU IMPURITY ON SI SUBSTRATE

Ahn, T. H.,Park, M. G.,Ryoo, Kun-Kul,Lee, Chong-Moo,Jeon-Tag, Hyeong-Tag 한국재료학회 1995 Fabrication and Characterization of Advanced Mater Vol.2 No.e1

Removal of Cu impurity from Si surfaces was investigated. Cu impurity on Si surface was contaminated interntionally by dipping into the standard 1ppm CuC$l_2$ chemical solution. The H-plasma treatments were conducted to remove Cu impurity depending on the rf powers, exposure times and remote distances. After these H-plasma treatments, Si surfaces were analyzed by using TXRF(total X-ray reflection fluorescence) and AFM(atomic force microscope). The concentration of Cu which was intentionally contaminated was about $10^{13}$ atoms/c$m^2$ and its surface roughness(root mean square) was around 2.7$\AA$. The Cu concentration and surface roughness were imporved after H-plasma cleaning. The Cu comcentration was reduced more than a factor of 10 and its surface roughness showed more than 30% improvement. This removal mechanism was tried to expain by applying the lift-off phenomena accompanied with the removal of a oxide and Cu impurity.

Enhanced Lysosomal Activity Is Involved in Bax Inhibitor-1-induced Regulation of the Endoplasmic Reticulum (ER) Stress Response and Cell Death against ER Stress. INVOLVEMENT OF VACUOLAR H⁺-ATPASE (V-ATPASE)

Lee, G.-H.,Kim, D.-S.,Kim, H.-T.,Lee, J.-W.,Chung, C.-H.,Ahn, T.,Lim, J.M.,Kim, I.-K.,Chae, H.-J.,Kim, H.-R. The American Society for Biochemistry and Molecula 2011 The Journal of biological chemistry Vol.286 No.28