한국재래 수닭에서 부화 후 혈청내 LH, Testosterone, Estrogen 과 IGF-1 농도의 변화

태현진,최철환,양홍현,김인식,장병귀,박영재 한국가금학회 2005 韓國家禽學會誌 Vol.32 No.2

Changes in serum estradiol, insulin-like growth factor-1, leuteinizing and testosterone levels, and leuteinizing hormone-stimulated testosterone production per testis in vitro from hatching to adulthood were studied in Korean native chickens of 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 21, 24, 28, 32, 44, 52 and 64 weeks (n=13 chickens per group) of age. The changes in the profiles of the levels in the incubation medium of luteinizing hormone-stimulated (100 ng/mL) testosterone secretion per testis in vitro, and the serum LH, testosterone, estradiol, and insulin-like growth factor-I were determined by radioimmunoassay. Serum estradiol levels were not significantly different at week 4 compared to that of 8, 12, 16, 21, 32, and 44. Significant decreases were observed at weeks 52 and 64. Serum leuteinizing hormone concentrations were not significantly different from 1 week to 12 weeks, increased gradually up to 32 weeks of age, and declined significantly thereafter; the highest value was at 32 weeks, and the lowest value was detected at 2 weeks of age. Serum insulin-like growth factor-I concentrations increased significantly from 1 week to 16 weeks, remained low and unchanged with advancing age. Serum testosterone concentrations were not significantly different at week 1 compared weeks 2, 4, 6, and 8. Significant increases were observed from 10 weeks to 32 weeks of age. Values at weeks 24, 28 and 32 and at weeks 32, 44, 52, and 64 were not significantly different. The highest value was at weeks 28 and the lowest value was detected at weeks 1 week. LH-stimulated testosterone production per testis in vitro increased gradually with age from 1 to 32 weeks and decreased significantly from 44 weeks to 64 weeks of age. 한국재래 닭에서 부화 후부터 성숙에 이르는 시기까지 황체형성호르몬 자극에 대한 고환내 testosterone 생성과 혈청내 황체형성호르몬, estradiol, IGF-I 및 testosterone 농도의 변화를 알아보기 위하여 부화 후 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 21, 28, 32, 44, 52 및 64주령(n=13마리/일령)의 한국재래 닭을 이용하여 방사면역측정법을 적용하여 이 연구를 수행하였다. 혈청내 estradiol의 농도는 4주령과 비교하여 8, 12, 16, 21, 32 및 44주령에서는 차이가 없었으나 52주령과 64주령에서는 큰 폭으로 증가하였다. 혈청내 황체 형성호르몬의 농도는 1~12주령까지는 유의성이 없었고 14주령부터 32주령까지 점진적으로 증가한 다음 유의성 있게 감소하였으며 32주령에 최고치와 2주령에 최저치를 나타내었다. 혈청내 IGF-I의 농도는 1~16주령까지는 유의성 있게 증가하였으나 이후에는 변화가 없이 낮게 유지되었다. 혈청내 testosterone 농도는 1, 2, 4 및 8주령에는 유의성이 없고 10~32주령까지 유의성 있게 증가하였으며 24~32주령 및 32~64주령에서는 유의성이 없었다. 황체형성호르몬 자극에 대한 고환내 testosterone 생성은 1~32주령까지 유의성 있게 증가하였고 44~64주령까지는 큰 폭으로 감소하였다.

한국재래 수탉에서 부화 후 혈청내 LH, Testosterone, Estrogen과 IGF-I 농도의 변화

태현진,장병귀,최철환,박영재,양홍현,김인식,Tae H. J.,Jang B. G.,Choi C. H.,Park Y. J.,Yang H. H.,Kim I. S. 한국가금학회 2005 韓國家禽學會誌 Vol.32 No.2

한국재래 자에서 부화 후부터 성숙에 이르는 시기까지 황체형성호르몬 자극에 대한 고환내 testosterone 생성과 혈청내 황체형성호르몬, estradiol, IGF-I 및 testosterone 농도의 변화를 알아보기 위하여 부화 후 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 21, 28, 32, 44, 52 및 64주령(n=13마리/일령)의 한국재래 닭을 이용하여 방사면역측정법을 적용하여 이 연구를 수행하였다. 혈청내 estradiol의 농도는4주령과 비교하여 8, 12, 16, 21, 32 및 44주령에서는 차이가 없었으나 52주령과 64주령에서는 큰 폭으로 증가하였다. 혈청내 황체 형성호르몬의 농도는 $1\~12$주령까지는 유의성이 없었고 14주령부터 32주령까지 점진적으로 증가한 다음 유의성 있게 감소하였으며 32주령에 최고치와 2주령에 최저치를 나타내었다. 혈청내 IGF-I의 농도는 $1\~16$주령까지는 유의성 있게 증가하였으나 이후에는 변화가 없이 낮게 유지되었다. 혈청내 testosterone농도는 1, 2, 4 및 8주령에는 유의성이 없고 $10\~32$주령까지 유의성있게 증가하였으며 $24\~32$주령 및 $32\~64$주령에서는 유의성이 없었다. 황체형성호르몬 자극에 대한 고환내 testosterone 생성은 $1\~32$주령까지 유의성 있게 증가하였고 $44\~64$주령까지는 큰 폭으로 감소하였다. Changes in serum estradiol, insulin-like growth factor-1, leuteinizing and testosterone levels, and leuteinizing hormone-stimulated testosterone production per testis in vitro from hatching to adulthood were studied in Korean native chickens of 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 21, 24, 28, 32, 44, 52 and 64 weeks (n=13 chickens per group) of age. The changes in the profiles of the levels in the incubation medium of luteinizing hormone-stimulated (100 ng/mL) testosterone secretion per testis in vitro, and the serum LH, testosterone, estradiol, and insulin-like growth factor-I were determined by radioimmunoassay. Serum estradiol levels were not significantly different at week 4 compared to that of 8, 12, 16, 21, 32, :md 44. Significant decreases were observed at weeks 52 and 64. Serum leuteinizing hormone concentrations were not significantly different from 1 week to 12 weeks, increased gradually up to 32 weeks of age, and declined significantly thereafter; the highest value was at 32 weeks, and the lowest value was detected at 2 weeks of age. Serum insulin-like growth factor-I concentrations increased significantly from 1 week to 16 weeks, remained low and unchanged with advancing age. Serum testosterone concentrations were not significantly different at week 1 compared weeks 2, 4, 6, and 8. Significant increases were observed from 10 weeks to 32 weeks of age. Values at weeks 24, 28 and 32 and at weeks 32, 44, 52, and 64 were not significantly different. The highest value was at weeks 28 and the lowest value was detected at weeks 1 week. LH-stimulated testosterone production per testis in vitro increased gradually with age from 1 to 32 weeks and decreased significantly from 44 weeks to 64 weeks of age.

Brown Norway rat에서 노화 간질세포에 미치는 LH와 thyroxine의 영향

태현진,박영재,최은영,양홍현,홍철운,안동춘,김인식 대한체질인류학회 2004 대한체질인류학회지 Vol.17 No.4

The present study was designed to investigate the possibility of restoring the testicular steroidogenic ability of the aged Brown Norway rats by administering luteinizing hormone (LH) and thyroxine (Thy). Rats of 3, 6, 12 months (M) of age (n = 8 per group) and four groups of 18 month old rats (n = 8 per group) were used. Eighteen month old rats were implanted subdermally with Alzet mini osmotic pumps containing saline (control), luteinizing hormone (LH, 24 μg/day), thyroxine (Thy, 5 μg/day) and LH and Thy (LH±Thy, 24 μg/day and 5 μg/day), respectively for four weeks (i.e testing was done at 19 months). The results showed that the testis volume was unchanged among all treatment groups. The number of Leydig cell per testis was not significantly different among all treatment groups. The average volume of a Leydig cell was significantly decreased at 12 months, and a further reduction was observed at 19 months (saline-treated); values for 19 month LH- and -LH±Thy-treated rats were not significantly lower than those at 3 and 6 months of age. Testosterone secretory capacity per testis and per Leydig cell in vitro were significantly reduced concomitantly with age advancement from 6 to 19 months (saline-treated) of age. These values of LH- and Thy-treated 19 month old rats were similar to those at 12 months. LH±Thy-treated rats were equally capable to 3 and 6 month old rats in producing testicular testosterone in vitro in response to LH. Serum testosterone was unchanged from 3M to 12 M rats but was reduced in 19M control rats. Both LH and Thy significantly raised these values above the 19M control levels, but they were still lower than the 3M through 12 M levels. Additionally, LH±Thy significantly raised the serum testosterone levels to those of 12M rats, but these values were significantly lower than those of 3M and 6M rats. In summary, the present study demonstrated that the exogenous supplementation of LH and Thy was effective in restoring the steroidogenic potential of the aged Leydig cells; the most effective treatment was LH±Thy, which upgraded the capacity of aged testes to those of 3 and 6 months. The present study was designed to investigate the possibility of restoring the testicular steroidogenic ability of the aged Brown Norway rats by administering luteinizing hormone (LH) and thyroxine (Thy). Rats of 3, 6, 12 months (M) of age (n = 8 per group) and four groups of 18 month old rats (n = 8 per group) were used. Eighteen month old rats were implanted subdermally with Alzet mini osmotic pumps containing saline (control), luteinizing hormone (LH, 24 μg/day), thyroxine (Thy, 5 μg/day) and LH and Thy (LH±Thy, 24 μg/day and 5 μg/day), respectively for four weeks (i.e testing was done at 19 months). The results showed that the testis volume was unchanged among all treatment groups. The number of Leydig cell per testis was not significantly different among all treatment groups. The average volume of a Leydig cell was significantly decreased at 12 months, and a further reduction was observed at 19 months (saline-treated); values for 19 month LH- and -LH±Thy-treated rats were not significantly lower than those at 3 and 6 months of age. Testosterone secretory capacity per testis and per Leydig cell in vitro were significantly reduced concomitantly with age advancement from 6 to 19 months (saline-treated) of age. These values of LH- and Thy-treated 19 month old rats were similar to those at 12 months. LH±Thy-treated rats were equally capable to 3 and 6 month old rats in producing testicular testosterone in vitro in response to LH. Serum testosterone was unchanged from 3M to 12 M rats but was reduced in 19M control rats. Both LH and Thy significantly raised these values above the 19M control levels, but they were still lower than the 3M through 12 M levels. Additionally, LH±Thy significantly raised the serum testosterone levels to those of 12M rats, but these values were significantly lower than those of 3M and 6M rats. In summary, the present study demonstrated that the exogenous supplementation of LH and Thy was effective in restoring the steroidogenic potential of the aged Leydig cells; the most effective treatment was LH±Thy, which upgraded the capacity of aged testes to those of 3 and 6 months.

한국재래 닭 고환 지지세포와 간질세포의 부화 후 발달에 관한 연구

태현진,장병귀,최철환,박영재,양홍현,김인식,Tae H. J.,Jang B. G.,Choi C. H.,Park Y. J.,Yang H. H.,Kim I. S. 한국가금학회 2005 韓國家禽學會誌 Vol.32 No.2

한국재래 닭에서 고환 지지세포와 간질세포의 발달유형을 명확하게 이해하기 위하여 부화 후 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 21, 28, 32, 44, 52 및 64주령(n:13마리/일령)의 한국재래 닭을 이용하여 이 연구를 수행하였다. 한국재래 닭의 고환은 $2.5\%$ glutaraldehyde를 이용하여 전신 관류 고정하고 조직 처리과정을 거쳐 Epon-araldite에 포매하였다. 초박절편기를 사용하여 $1{\mu}m$로 절편한 다음 methylene blue로 염색하여 형태 계측을 하였다. 부화후 1주령의 한국재래 닭 고환의 평균용적은 $0.148\;cm^3$이었고 점진적으로 증가하여 21주령에는 $3.93\;cm^3$이고 21주령부터 64주령까지는 변화가 없었다. 곱슬정세관의 용적 치밀도는 1주령에 $32.6\%$이었으나 점차적으로 증가하여 64주령에서는 92.89이었다. 1주령의 한국재래 닭에서 고환 간질조직은 고환실질의 $67.4\%$를 나타내었고 이러한 비율은 성장하는 동안에 점차적으로 감소하여 64주령에 $7.11\%$를 나타내었다. 간질세포의 용적 치밀도는 1주령부터 14주령까지 단계적으로 감소하였고 이후에는 변화가 관찰되지 않았다. 이와는 다르게 지지세포의 용적 치밀도는 1주령에 $3.4\%$를 차지하고 있었고 점진적으로 증가하여 18주령에 $10.79\%$이었으며 이후에는 변화가 없었다. 고환조직내 지지세포와 간질세포의 절대용적은 일령에 1주령부터 24주령까지는 유의성 있게 증가하였으나 24주령부터 64주령까지는 차이가 없었다. 고환조직당 간질세포의 총 숫자는 1주령부터 21주령까지는 유의성 있게 증가하였으나 그 이후에는 변화가 없었고 지지세포의 숫자는 1주령부터 14주령까지 점진적으로 증가하였고 그 이후의 주령에서는 변화가 없었다. Morphometric changes in testicular Sertoli and Leydig cells from hatching to adulthood were studied using Korean native chickens of 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 21, 24, 28, 32, 44, 52 and 64 weeks (n=13 chickens per group) of age. The objective of this study was to understand the developmental phase of the Sertoli and Leydig cells with age. Testis of chickens was fixed by whole body perfusion using a fixative containing $2.5\%$ glutaraldehyde in cacodylate buffer, processed and embedded in Epon-araldite. Using 1 Um sections stained with methylene blue-azure II, qualitative and quantitative (stereological) morphological studies were performed. The average volume of a testis of 1 week old Korean native chickens was determined as $0.148\;cm^3$ and the parameter increased linearly from 1 week to 21 weeks days $(28.86\;cm^3)$, and did not change from 21 weeks to 64 weeks. The volume density of the seminiferous tubules increased with age from $32.6\%$ at week 1 to $92.89\%$ at week 64. The volume density of the interstitium represents $67.4\%$ of the testicular parenchyma at week 1. This proportion progressively diminished during development to reach a value of $7.11\%$ at week 64. The volume density of the Leydig cells decreased almost linearly from 1 week $(4.9\%)$ to 14 weeks $(1.7\%)$ and remained unchanged thereafter. In contrast, the Sertoli cells occupied a volume density of $3.4\%$ at week 1, increased progressively up to 18 weeks of age $(10.79\%)$ and remained unchanged thereafter. The absolute volume of the Leydig and Sertoli cells per testis increased significantly from week 1 to week 21 but did not change significantly from week 24 to week 64. The number of Leydig cells per testis increased almost linearly from 1 week to 21 weeks, remained high and unchanged with advancing age. The number of Sertoli cells per testis increased gradually with age from 1 week to 14 weeks and remained unchanged thereafter.

수컷 흰쥐에서 심근경색이 번식기능에 미치는 영향

태현진,문진희,유일정,박진호,이영훈,안동춘,강형섭,박영재,양홍현,김인식 대한체질인류학회 2006 대한체질인류학회지 Vol.19 No.3

The present study was to investigate in more detail the changes of reproductive function in the male rat following myocardial infarction (MI). Ligation of the left coronary artery was performed in male Sprague-Dawley male rats at 60 days of age. Control rats were obtained sham-operated animals. MI rats were sacrificed at 1, 3, 5, 7, 14, and 30 day after ligation of left anterior descending coronary artery. Control rats were sacrificed on 30 day after thoracotomy. Myocardial infarct size was assessed by planimetry and perimetry. Testes of rats were fixed by whole body perfusion using a fixative containing 2.5% glutaraldehyde in cacodylate buffer, processed and embedded in Epon-araldite. Using 1 µm sections stained with methylene blue-azure II, qualitative and quantitative (stereological) morphological studies were performed. Testosterone levels in the incubation medium of luteinizing hormone-stimulated (100 ng/mL) testosterone secretion per testis in vitro, and in serum were determined by radioimmunoassay. Sperm production was measured by routine technique. Mean infarct size was 29.5~33.5% of the left ventricle after coronary occlusion in experimental groups. No changes were observed in testis volume, absolute volume of Leydig cell, Leydig cell size, and number of Leydig cell per testis in MI rats compared to sham-operated animals. Serum testosterone, LH-stimulated testicular testosterone production, and daily sperm production in MI rats were not significantly different (P¤0.05) from sham-operated animals. These results demonstrate that under the experimental conditions employed here, experimental chronic myocardial infarction does not exert adverse effects on the reproductive function of male rats. 수컷 흰쥐에서 실험적 심근경색에 따른 번식기능의 변화를 규명하기 위하여 본 연구를 수행하였다. 60일 령의 수컷 흰쥐를 왼쪽관상동맥을 결찰하여 심근경색 모델을 만들었고 개흉하여 sham 대조군을 삼았다. 실험군은 심근경색 유발 후 1, 3, 5, 7, 14 및 30일에 희생시켰고 대조군은 개흉 후 30일에 희생시켰다. 심근경색의 크기는 planimetry 및 perimetry 법을 적용하여 측정하였다. 흰쥐의 고환은 2.5% glutaraldehyde를 이 용하여 전신관류 고정하고 조직 처리과정을 거쳐 epon-araldite에 포매하였다. 초박절편기를 사용하여 1 µm로 절 편한 다음 methylene blue로 염색하여 일반적인 조직의 변화상과 형태계측을 하였다. 고환을 배양한 후 황체형성 호르몬 자극에 대한 고환내 테스토스테론 생성과 혈청내 테스토스테론 농도는 방사면역측정법을 적용하여 측정 하였으며, 일반적인 방법에 따라서 정자생성을 측정하여 다음과 같은 결과를 얻었다. 관상동맥을 결찰한 실험군에서 평균 심근경색의 크기는 왼쪽심실의 29.5~33.5%이었다. 심근경색을 유발시킨 모든 실험군에서 고환 용적, 간질세포의 절대용적, 간질세포의 크기 및 고환 조직 당 간질세포의 총 숫자는 대조 군과 비교하여 차이가 관찰되지 않았다. 심근경색 실험동물에서 혈청내 테스토스테론 농도, 황체형성호르몬 자극 에 대한 고환내 테스토스테론 농도 및 1일 정자생성이 대조군과 비교하여 큰 차이를 보이지 않았다. 본 연구의 결과는 수컷 흰쥐에서 실험적 만성 심근경색은 번식기능에 해로운 영향이 없음을 알 수 있었다.

흰쥐에서 출생후 고환 간질세포의 발생

태현진,이여광,박병용,박영재,최은영,이영훈,안동춘,강형섭,박상열,박수현,이존화,양홍현,김인식,Tae, Hyun-Jin,Li, Li-Guang,Park, Byung-Yong,Park, Young-Jae,Choi, Eun-Young,Lee, Young-Hoon,Ahn, Dong-Choon,Kang, Hyung-Sub,Park, Sang-Youel,Park, S 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.4

Changes in the rat testis interstitium from birth to adulthood were studied using Sprague Dawley rats of 1, 7, 14, 21, 28, 40, 60, and 90 days of age to investigate Leydig cell differentiation. In addition, serum testosterone concentrations and luteinizing hormone stimulated (LH; 100 ng/ml) testosterone secretory capacity per testis in vitro were determined via radioimmunoassay. Fetal Leydig cells were present in rat testes from birth to 21 days, and they were only steroidogenic cells in the testis at days 1 and 7. The average volume of a fetal Leydig cell and the absolute volume of fetal Leydig cell per testis were similar at all ages of experimental groups except at day 21 when lower values were observed for both parameters. The number of fetal Leydig cells per testis remained constant from birth through 21 days. Adult Leydig cells were recognized at day 14 and their absolute volume and number per testis increased linearly from 14 to 90 days. The average volume of an adult Leydig cell increased significantly with age and reached maximum size by 60 days of age where the volume was nearly three times bigger than that of at day 14. Total testosterone production per testis in vitro and serum testosterone concentrations were not significantly different at day 1 compared with 7, 14, and 21 days of age. Significant increases were observed at days 40 and 60. Values at days 60 and 90 were not significantly different.

토끼에서 출생 후 고환간질세포의 발생에 관한 연구

태현진,박영재,강형섭,김남수,박상열,양홍현,안동춘,김인식,Tae, Hyun-Jin,Park, Young-Jae,Kang, Hyung-Sub,Kim, Nam-Soo,Park, Sang-Youel,Yang, Hong-Hyun,Ahn, Dong-Choon,Kim, In-Shik 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.3

Changes in the rabbit Leydig cell from birth to adulthood were studied in New Zealand white rabbits of 1, 7, 21, 35, 49, 70, 105, 147, 196, and 252 days (n = 8 rabbits per group) of age. The objectives of this study were to understand the fate of the fetal Leydig cells, to determine the changes in serum testosterone levels, and leutenizing hormone-stimulated testosterone production per testis in vitro, and to quantify adult Leydig cells by number and average volume with age. Testes of rabbits were fixed by whole body perfusion using a fixative containing 2.5% glutaraldehyde in cacodylate buffer, processed and embedded in Epon-araldite. Using $1{\mu}m$ sections stained with methylene blue-azure II, qualitative and quantitative (stereological) morphological studies were performed. Testosterone levels in the incubation medium of luteinizing hormone-stimulated (100 ng/ml) testosterone secretion per testis in vitro, and in serum were determined by radioimmunoassay. The average volume of a testis of 1-day-old rabbits was determined as $0.0073cm^3$ and the parameter increased linearly from birth to 252 days ($3.93cm^3$). The volume density of the seminiferous tubules increased with age from 33.76% at day 1 to 88.2% at day 252. The volume density of the interstitium represents 66.24% of the testicular parenchyma at day 1. This proportion progressively diminished during development to reach a value of 11.8% at day 252. The volume density of Leydig cells increased almost linearly from birth (0.001%) to 252 days (2.62%). Leydig cell mass per testis increases from 0.0012 mg to 0.25 mg between days 1 and 35, from 2.66 mg to 44.3 mg between days 49 and 105 and from 65.42 mg and 102.9 mg between days 147 and 252. The absolute numbers of adult Leydig cells per testis increased linearly from birth to 252 days. The average volume of adult Leydig cell on days 1, 7, 21 and 35 was not significantly different; a gradual and continued increase was observed thereafter, reaching a 3-fold increase at 196 and 252 days. Serum testosterone concentrations were not significantly different at day 1 compared days 7, 21, 35. Significant increases were observed at days 49 and 70. Values at days 70 and 105 and days 147, 196, and 252 were not significantly different. LH-stimulated testosterone production per testis in vitro was significantly different at day 1 compared days 7, 21, 35. Significant increases were observed at days 49 and 70. Hormonal values at days 105, 147, 196, and 252 were not significantly different. These data suggested Leydig cell developmental phase can be classified: a neonatal phase (1-7 days), a prepubertal phase (14-49 days) and an adult phase (70-252 days). Immature and mature adult Leydig cells, initially detected at days 7 and 49, respectively, and mature adult Leydig cells were abundant Leydig cell type according to the number and absolute volume per testis form day 49 onwards.

한국재래닭 고환 지지세포와 간질 세포의 부화 후 발달에 관한 연구

태현진,최철환,양홍현,김인식,장병귀,박영재 한국가금학회 2005 韓國家禽學會誌 Vol.32 No.2

Morphometric changes in testicular Sertoli and Leydig cells from hatching to adulthood were studied using Korean native chickens of 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 21, 24, 28, 32, 44, 52 and 64 weeks (n=13 chickens per group) of age. The objective of this study was to understand the developmental phase of the Sertoli and Leydig cells with age. Testis of chickens was fixed by whole body perfusion using a fixative containing 2.5% glutaraldehyde in cacodylate buffer, processed and embedded in Epon-araldite. Using 1 μm sections stained with methylene blue-azure II, qualitative and quantitative(stereological) morphological studies were performed. The average volume of a testis of 1 week old Korean native chickens was determined as 0.148 cm3 and the parameter increased linearly from 1 week to 21 weeks days (28.86 cm3), and did not change from 21 weeks to 64 weeks. The volume density of the seminiferous tubules increased with age from 32.6% at week 1 to 92.89% at week 64. The volume density of the interstitium represents 67.4% of the testicular parenchyma at week 1. This proportion progressively diminished during development to reach a value of 7.11% at week 64. The volume density of the Leydig cells decreased almost linearly from 1 week (4.9%) to 14 weeks (1.7%) and remained unchanged thereafter. In contrast, the Sertoli cells occupied a volume density of 3.4% at week 1, increased progressively up to 18 weeks of age (10.79%) and remained unchanged thereafter. The absolute volume of the Leydig and Sertoli cells per testis increased significantly from week 1 to week 21 but did not change significantly from week 24 to week 64. The number of Leydig cells per testis increased almost linearly from 1 week to 21 weeks, remained high and unchanged with advancing age. The number of Sertoli cells per testis increased gradually with age from 1 week to 14 weeks and remained unchanged thereafter. 한국재래 닭에서 고환 지지세포와 간질세포의 발달유형을 명확하게 이해하기 위하여 부화 후 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 21, 28, 32, 44, 52 및 64주령(n=13마리/일령)의 한국재래 닭을 이용하여 이 연구를 수행하였다. 한국재래 닭의 고환은 2.5% glutaraldehyde를 이용하여 전신 관류 고정하고 조직 처리과정을 거쳐 Epon-araldite에 포매하였다. 초박절편기를 사용하여 1μm로 절편한 다음 methylene blue로 염색하여 형태 계측을 하였다. 부화후 1주령의 한국재래 닭 고환의 평균용적은 0.148 cm3이었고 점진적으로 증가하여 21주령에는 3.93 cm3이고 21주령부터 64주령까지는 변화가 없었다. 곱슬정세관의 용적 치밀도는 1주령에 32.6%이었으나 점차적으로 증가하여 64주령에서는 92.89이었다. 1주령의 한국재래 닭에서 고환 간질조직은 고환실질의 67.4%를 나타내었고 이러한 비율은 성장하는 동안에 점차적으로 감소하여 64주령에 7.11%를 나타내었다. 간질세포의 용적 치밀도는 1주령부터 14주령까지 단계적으로 감소하였고 이후에는 변화가 관찰되지 않았다. 이와는 다르게 지지세포의 용적 치밀도는 1주령에 3.4%를 차지하고 있었고 점진적으로 증가하여 18주령에 10.79%이었으며 이후에는 변화가 없었다. 고환조직내 지지세포와 간질세포의 절대용적은 일령에 1주령부터 24주령까지는 유의성 있게 증가하였으나 24주령부터 64주령까지는 차이가 없었다. 고환조직당 간질세포의 총 숫자는 1주령부터 21주령까지는 유의성 있게 증가하였으나 그 이후에는 변화가 없었고 지지세포의 숫자는 1주령부터 14주령까지 점진적으로 증가하였고 그 이후의 주령에서는 변화가 없었다.

토끼에서 고환 사이질조직의 출생 후 성 성숙에 이르는 변화

태현진(Hyun-Jin Tae),김인식(In-Shik Kim) 대한체질인류학회 2012 대한체질인류학회지 Vol.25 No.1

토끼에서 출생 후부터 성 성숙에 이르는 시기까지 고환 사이질조직의 변화를 알아보기 위하여 출생 후 1,7,21,35,49,70,105,147,196 및 252일령 (n=8마리/일령)의 토끼를 이용하였다. 토끼의 고환을 2.5% glutaraldehyde를 이용하여 전신 관류고정하고 조직 처리과정을 거쳐 Epon-araldite에 포매하였다. 초박절편기를 사용하여 1 ㎛로 절편한 다음 methylene blue로 염색하여 고환사이질조직을 이루고 있는 각 구조물의 일반적인 변화상과 형태계측학적인 방법을 적용하여 고환 사이질조직의 변화를 관찰하였다. 1일령의 토끼에서 고환 사이질조직은 고환실질의 66.3%를 나타내었고 성장하면서 점차적으로 감소하여 252일령에 11.8%를 나타내었다. 성숙 사이질세포는 7일령에 처음 관찰되었고 이 시기에 용적치밀도는 0.001%이었으며 성장하면서 증가하여 252일령에는 2.6%에 도달하였다. 사이질세포, 중간엽세포, 혈관내피세포, 혈관주위세포 및 근육모양세포의 절대용적은 출생 후부터 252일령까지 증가하였다(P<0.05). 고환조직 당 혈관내피세포, 혈관 주위세포, 근육모양세포, 성숙 사이질세포 및 중간엽세포의 총 숫자는 일령에 따라서 점진적으로 증가하였고 출생 후부터 252일령까지 성숙 사이질세포의 생성률이 중간엽세포의 생성률보다 높게 나타났다(P<0.05). 중간엽세포의 평균용적은 1일령부터 70일령까지 증가하였으며 70일령에 최고치를 나타내었다. 성숙 사이질세포의 평균용적은 1, 7, 21 및 35일령에는 유의성이 없었으나 이 후 점차적으로 계속 증가하여 196일령과 252일령에는 3배 증가하였다(P<0.05). 고환내 총 정자생성과 1일 정자생성은 105일령부터 252일령까지 유의성 있게 증가하였다(P< 0.05). 이러한 결과는 토끼에서 출생 후부터 성 성숙 시기까지 고환 사이질조직을 이루고 있는 각종 세포의 용적치 밀도, 절대용적, 평균용적 및 고환내 총 숫자의 변화는 정자발생과 밀접한 연관이 있음을 확인하였다. Changes in the testis interstitium from birth to adulthood were studied in New Zealand white rabbits of 1, 7, 21, 35, 49, 70, 105, 147, 196, and 252 days (n=8 per group) of age. Testes of rabbits were fixed by whole body perfusion using a fixative containing 2.5% glutaraldehyde in cacodylate buffer, processed and embedded in Epon-araldite. Using 1 sections stained with methylene blue-azure II, qualitative and quantitative (stereological) morphological studies were performed. Sperm production was measured by routine technique. The volume density of the interstitium represents 66.3% of the testicular parenchyma at day 1. Adult Leydig cells initially detected at day 7(0.001%), increased progressively to reach a volume density of 2.6% by day 252. The absolute volume of mesenchymal cells, endothelial cells, pericytes, and myoid cells increased from birth to 252 days. The number of endothelial cells, pericytes, and myoid cells per testis increased gradually with age. The absolute numbers of adult Leydig cells and mesenchymal cells per testis increased linearly from birth to 252 days. The rate of production of adult Leydig cells was greater than that of mesenchymal cells in the postnatal rabbit testis through 252 days. The average volume of a mesenchymal cell increased from day 1 to day 70 and declined thereafter; the highest value was at day 70. Total sperm production and daily sperm production per testis increased significantly from 105 to 252 days of age. These results clarified the pattern of changes in the testis interstitium in rabbits from birth to adulthood and correlation these events with spermatogenesis.

한우 고환 간질세포의 형태계측학적 연구

태현진 ( Hyun-jin Tae ),안동춘 ( Dong-choon Ahn ),이영덕 ( Young-deok Lee ),나성진 ( Sung-jin Na ),박병용 ( Byung-yong Park ),김인식 ( In-shik Kim ) 한국가축위생학회 2016 韓國家畜衛生學會誌 Vol.39 No.3

Changes in the Leydig cell from pre-puberty to adulthood were studied in Korean native cattle. Eight groups of male cattle aged 14, 17, 20, 25, 30, 35, 40 and 104 weeks (n=7 cattle per group) after birth were used. The purpose of this study was to obtain quantitative information on the Leydig cell of the Korean native cattle during postnatal development. Testes of cattle were fixed by perfusion using a fix-ative containing 2.5% glutaraldehyde in cacodylate buffer, processed and embedded in Epon-araldite. Using 1 μm section stained with methylene blue-azure II, qualitative and quantitative (stereological) morphological studies were performed. The average diameter of seminiferous tubules gradually increased with age from 14 (75.56 μm) to 104 weeks (298.9 μm). The volume density of the seminiferous tubules increased with age from 54.2% at week 14 to 76.9% at week 104. The volume density of the inter-stitium represents 45.52% at week 14. This proportion progressively diminishes during development to reach a value of 23.14% at week 104. The volume density of Leydig cells decreased almost linearly from 14 (20.71%) to 104 weeks (5.28%). The absolute volume of Leydig cells per testis increased sig-nificantly from 14 to 104 weeks. The number of Leydig per testis have almost linearly increased from 14 to 104 weeks. The average volume of a Leydig cell reached maximum size by 104 weeks (2553 μm³). These data suggested development of Leydig cell can be classified as the fetal and immature adult Leydig cells (14∼35 weeks), and the adult Leydig cells (40∼104 weeks).