Efficacy of a single-dose regimen of inactivated whole-cell oral cholera vaccine: results from 2 years of follow-up of a randomised trial

Qadri, Firdausi,Ali, Mohammad,Lynch, Julia,Chowdhury, Fahima,Khan, Ashraful Islam,Wierzba, Thomas F,Excler, Jean-Louis,Saha, Amit,Islam, Md Taufiqul,Begum, Yasmin A,Bhuiyan, Taufiqur R,Khanam, Farhana Elsevier 2018 LANCET INFECTIOUS DISEASES Vol.18 No.6

<P><B>Summary</B></P> <P><B>Background</B></P> <P>A single-dose regimen of inactivated whole-cell oral cholera vaccine (OCV) is attractive because it reduces logistical challenges for vaccination and could enable more people to be vaccinated. Previously, we reported the efficacy of a single dose of an OCV vaccine during the 6 months following dosing. Herein, we report the results of 2 years of follow-up.</P> <P><B>Methods</B></P> <P>In this placebo-controlled, double-blind trial done in Dhaka, Bangladesh, individuals aged 1 year or older with no history of receipt of OCV were randomly assigned to receive a single dose of inactivated OCV or oral placebo. The primary endpoint was a confirmed episode of non-bloody diarrhoea for which the onset was at least 7 days after dosing and a faecal culture was positive for <I>Vibrio cholerae</I> O1 or O139. Passive surveillance for diarrhoea was done in 13 hospitals or major clinics located in or near the study area for 2 years after the last administered dose. We assessed the protective efficacy of the OCV against culture-confirmed cholera occurring 7–730 days after dosing with both crude and multivariable per-protocol analyses. This trial is registered at ClinicalTrials.gov, number NCT02027207.</P> <P><B>Findings</B></P> <P>Between Jan 10, 2014, and Feb 4, 2014, 205 513 people were randomly assigned to receive either vaccine or placebo, of whom 204 700 (102 552 vaccine recipients and 102 148 placebo recipients) were included in the per-protocol analysis. 287 first episodes of cholera (109 among vaccine recipients and 178 among placebo recipients) were detected during the 2-year follow-up; 138 of these episodes (46 in vaccine recipients and 92 in placebo recipients) were associated with severe dehydration. The overall incidence rates of initial cholera episodes were 0·22 (95% CI 0·18 to 0·27) per 100 000 person-days in vaccine recipients versus 0·36 (0·31 to 0·42) per 100 000 person-days in placebo recipients (adjusted protective efficacy 39%, 95% CI 23 to 52). The overall incidence of severe cholera was 0·09 (0·07 to 0·12) per 100 000 person-days versus 0·19 (0·15 to 0·23; adjusted protective efficacy 50%, 29 to 65). Vaccine protective efficacy was 52% (8 to 75) against all cholera episodes and 71% (27 to 88) against severe cholera episodes in participants aged 5 years to younger than 15 years. For participants aged 15 years or older, vaccine protective efficacy was 59% (42 to 71) against all cholera episodes and 59% (35 to 74) against severe cholera. The protection in the older age groups was sustained throughout the 2-year follow-up. In participants younger than 5 years, the vaccine did not show protection against either all cholera episodes (protective efficacy −13%, −68 to 25) or severe cholera episodes (−44%, −220 to 35).</P> <P><B>Interpretation</B></P> <P>A single dose of the inactivated whole-cell OCV offered protection to older children and adults that was sustained for at least 2 years. The absence of protection of young children might reflect a lesser degree of pre-existing natural immunity in this age group.</P> <P><B>Funding</B></P> <P> <ce:grant-sponsor id='gs3' sponsor-id='http://dx.doi.org/10.13039/100000865' xlink:type='simple' xlink:role='http://www.elsevier.com/xml/linking-roles/grant-sponsor'>Bill & Melinda Gates Foundation</ce:grant-sponsor> to the International Vaccine Institute.</P>

Effect of Production Phase on Growth, Enzyme Activities and Feed Selection of Broilers Raised on Vegetable Protein Diet

Hossain, M.A.,Islam, A.F.,Iji, P.A. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.11

This study consisted of two experiments, conducted to assess the impact of phase at which vegetable protein (VP) diets are introduced to broiler chicks, and preference of birds for diets based on soybean or canola meal (CM). Two hundred and ten day-old Cobb 500 chicks were randomly distributed into five dietary groups in the main experiment. One group was fed on animal protein (AP) diet all through to 21 days of age; two other groups were started on AP diet for 7 days and then switched to diets containing soybean meal (AP-SBM) or AP-CM, while two other diets (SBM-AP and CM-AP) were started on one of the VP diets for 7 days and then switched to AP diet. A sub-experiment on thirty birds raised on a commercial diet to 7 days was used in a feed selection test to quantify the preference of birds for the diets containing mainly CM or SBM. Chicks were reared under similar care and management conditions and the diets were iso-caloric and iso-nitrogenous. Results of the main experiment showed that chicks on CM-AP diet ate more (p<0.05) than those on the other diets up to day 7. Body weight gain was highest (p<0.001) on the AP-SBM diet while birds on the CM-AP diet weighed the least at 7 d. Feed intake, body weight gain, feed conversion ratio, mortality, bone growth, visceral organ development, and activities of digestive enzymes were similar between the groups from hatch to 21 days of age. Results of the second sub-experiment showed that chicks preferred the CM-based diets to the SBM-based diets at 8 to 14 d (p<0.001) and 15 to 21 d (p<0.01) when given a choice. Overall, the birds were not affected by the nature of the starter diet although they tended to prefer the canola to soybean diets.

HEMATOLOGICAL STUDIES ON CATTLE EXPOSED TO Fasciola gigantica INFESTATION

Taimur, M.J.F.A.,Halder, A.K.,Chowdhury, S.M.Z.H.,Akhter, N.,Islam, M.S.,Kamal, A.H.M.,Islam, K.S. Asian Australasian Association of Animal Productio 1993 Animal Bioscience Vol.6 No.2

Hematological value were determined from 140 Fasciola gigantica infested and 138 non-infested apparently healthy (control) zebu cattle from eight different sites of Bangladesh. The F. gigantica infested cattle have experienced of normocytic normochromic anemia indicated by the significant (p<0.01) decline of total erythrocyte count, hemoglobin level, packed cell volume and mean corpuscular hemoglobin concentration and significant (p<0.01) increase of erythrocyte sedimentation rate, as compared with non-infested control animals. Values of mean corpuscular volume and mean corpuscular hemoglobin content were not differed significantly between the infested and control animals. There observed significantly (p<0.01) higher eosinophil and neutrophil counts and significantly (p<0.01) lower lymphocyte counts in fasciola infested cattle in comparison with the control animals.

Physico-chemical properties of late-incubation egg amniotic fluid and a potential in ovo feed supplement

A.A. Omede,M.M. Bhuiyan,A.F. Islam,P.A. Iji 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.8

Objective: This study explored the physico-chemical properties of late-incubation egg amniotic fluid and a potential in ovo feed (IOF) supplement. Methods: Amniotic fluid was collected from broiler breeders (Ross 308, 51 weeks and Cobb 500, 35 weeks) on day 17 after incubation. A mixture of high-quality soy protein supplement – Hamlet Protein AviStart (HPA) was serially diluted in MilliQ water to obtain solutions ranging from 150 to 9.375 mg/mL. The mixtures were heat-treated (0, 30, 60 minutes) in a waterbath (80°C) and then centrifuged to obtain supernatants. The amniotic fluid and HPA supernatants were analysed for their physico-chemical properties. Results: Only viscosity and K+ were significantly (p<0.05) different in both strains. Of all essential amino acids, leucine and lysine were in the highest concentration in both strains. The osmolality, viscosity and pCO2 of the supernatants decreased (p<0.05) with decreasing HPA concentration. Heat treatment significantly (p<0.05) affected osmolality, pH, and pCO2, of the supernatants. The interactions between HPA concentration and heat treatment were significant with regards to osmolality (p<0.01), pH (p<0.01), pCO2 (p<0.05), glucose (p<0.05), lactate (p<0.01) and acid-base status (p<0.01) of HPA solutions. The Ca2+, K+, glucose, and lactate increased with increasing concentration of HPA solution. The protein content of HPA solutions decreased (p<0.05) with reduced HPA solution concentrations. The supernatant from 150 mg/mL HPA solution was richest in glutamic acid, aspartic acid, arginine and lysine. Amino acids concentrations were reduced (p<0.05) with each serial dilution but increased with longer heating. Conclusion: The values obtained in the primary solution (highest concentration) are close to the profiles of high-protein ingredients. This supplement, as a solution, hence, may be suitable for use as an IOF supplement and should be tested for this potential.

A Non-Linear Exponential(NLINEX) Loss Function in Bayesian Analysis

Islam, A.F.M.Saiful,Roy, M.K.,Ali, M.Masoom The Korean Data and Information Science Society 2004 한국데이터정보과학회지 Vol.15 No.4

In this paper we have proposed a new loss function, namely, non-linear exponential(NLINEX) loss function, which is quite asymmetric in nature. We obtained the Bayes estimator under exponential(LINEX) and squared error(SE) loss functions. Moreover, a numerical comparison among the Bayes estimators of power function distribution under SE, LINEX, and NLINEX loss function have been made.

Multifunctional Polymeric Nanocurcumin for Cancer Therapy

Shehzad, A.,Ul-Islam, M.,Wahid, F.,Lee, Y.S. American Scientific Publishers 2014 Journal of nanoscience and nanotechnology Vol.14 No.1

Nanotechnology-based drug delivery systems have the potential to enhance the efficacy of poorly soluble systemic drugs. Curcumin, a yellow pigment isolated from turmeric, possesses a wide range of pharmacological activities, including anticancer effects. The anticancer potential of curcumin is mediated through the inhibition and modulation of several intracellular signaling pathways, as confirmed in various in vitro and in vivo cancer studies. However, clinical application of dietary curcumin for the treatment of cancer and other chronic diseases have been hindered by poor bioavailability, due to low systemic solubility as well as rapid metabolism and elimination from the body. Different techniques for sustained and efficient curcumin delivery, including nanoparticles, liposomes, micelles, phospholipids, and curcumin-encapsulated polymer nanoparticles are the focus of this study. Previous studies have shown that nanocurcumin has improved anticancer effects as compared to normal curcumin formulations. Among nanoformulations, few composite nanosystems have the simultaneous properties of therapeutic activity and multifunctional nanoparticles as enhanced image contrast agents. We also address the challenges to the development of nanocurcumin delivery platforms by enhancing a steady aqueous dispersion state. Further studies are needed using preclinical and clinical cancer models to recommend nanocurcumin as a drug of choice for cancer therapy.

Circulating Cell-free miRNA Expression and its Association with Clinicopathologic Features in Inflammatory and Non-Inflammatory Breast Cancer

Hamdi, K,Blancato, J,Goerlitz, D,Islam, MD,Neili, B,Abidi, A,Gat, A,Ayed, F Ben,Chivi, S,Loffredo, CA,Jillson, I,Elgaaied, A Benammar,Marrakchi, R Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.4

Recent discovery showing the presence of microRNAs (miRNAs) in the circulation sparked interest in their use as potential biomarkers. Our previous studies showed the diagnostic potential of miR-451 as a serological marker for inflammatory breast cancer (IBC), miR-337-5p and miR-30b for non-inflammatory breast cancer (non-IBC). The aim of this study is to investigate the prognostic values of circulating miRNAs by comparing the amounts of 12 circulating miRNAs in the serum of IBC and non-IBC from Tunisian breast cancer patients, and by determinating whether correlated pairs of miRNAs could provide useful information in the diagnosis of IBC and non-IBC patients. TaqMan qPCR was performed to detect circulating expression of miRNAs in serum of 20 IBC, 20 non-IBC and 20 healthy controls. Nonparametric rank Spearman rho correlation coefficient was used to examine the prognostic value of miRNAs and to assess the correlation profile between miRNAs expression. Further, a large number of miRNAs were highly correlated (rho>0.5) in both patients groups and controls. Also, the correlations profiles were different between IBC, non-IBC and healthy controls indicating important changes in molecular pathways in cancer cells. Our results showed that miR-335 was significantly overexpressed in premenopausal non-IBC patients; miR-24 was significantly overexpressed in non-IBC postmenopausal patients. Patients with previous parity had higher serum of miR-342-5p levels than those without. Furthermore, patients with HER2+ IBC present lower serum levels of miR-15a than patients with HER2-disease. Together, these results underline the potential of miRNAs to function as diagnostic and prognostic markers for IBC and non-IBC, with links to the menopausal state, Her2 status and parity.

Aspects of Structural and Multiferroic Properties of A- (15% Gd) and B-Site (5–15% Cr) Doped Perovskite BiFeO3 Nanoparticles

M. N. Hossain,M. A. Matin,M. A. Islam,M. M. Rhaman,M. A. Hakim,M. F. Islam 한국전기전자재료학회 2021 Transactions on Electrical and Electronic Material Vol.22 No.4

This report focuses on the investigation of multiferroic properties of 15% Gd and (5–15) % Cr co-doped Bismuth Ferrite (BFO) nanoparticles prepared by sol–gel method. The synthesized pure BFO has shown to be R3c rhombohedral crystal structure which partially transforms to Pnma orthorhombic crystal structure with increasing Cr dopant in co-doped samples. The crystallite size was found to vary from 68 to 22 nm depending on doping level. SEM images exhibited uniform nanoparticles and the average size of particles was shown to vary from 263 to 114 nm which is comparatively higher than that obtained from XRD results due to agglomeration eff ects. Ferroelectric tests exhibited a signifi cant reduction in polarization in doped BFO under an applied fi eld of ± 15 kV/cm. In contrast, the magnetic measurement showed a substantial improvement in ferromagnetic properties of the synthesized samples compared to un-doped one. A prominent exchange anisotropy eff ect was also observed in all the samples at room temperature. In addition, co-doped BFO samples demonstrated a signifi cant reduction in band gap energy from 1.88 to 1.68 eV.

Production, Characterization and Physico-mechanical Properties of Bacterial Cellulose from Industrial Wastes

Khattak, W. A.,Khan, T.,Ul-Islam, M.,Wahid, F.,Park, J. K. Springer Science + Business Media 2015 Journal of polymers and the environment Vol.23 No.1

Detection of Clostridium perfringens and its toxinotypes by enzyme linked immunosorbent assay from enterotoxaemic goats in Bangladesh

Islam, K.B.M.S.,Rahman, M.S.,Ershaduzzaman, Md.,Taimur, M.J.F.A.,Jang, Hyung-Kwan,Song, Hee-Jong The Korean Society of Veterinary Service 2010 韓國家畜衛生學會誌 Vol.33 No.1

An enzyme-linked immnnosorbent assay (ELISA) has been performed for the detection of the prevailing toxinotypes of Clostridium perfringens obtained from conventional culturing of intestinal contents of goats which have died of suspected enterotoxaemia. The test was found effective to detect the toxins as well as types of the organism with less time and labor. The most prevailing type of C. perfringens causing enterotoxaemia in goat was C. perfringens type D (68.75%) and followed by C. perfringens type B (25%) and C (6.25%). No C. perfringens type A was detected. This study showed an intelligible picture of prevailing toxinotypes of C. perfringens in goats in Bangladesh. The use of the ELISA for the detection of clostridial types and toxins allows the differential diagnosis of C. perfringens types A, B, C and D enterotoxaemias from samples of intestinal contents and the typing of cultures of C. perfringens.