Mitochondrial DNA Diversity and Origin of Red Chittagong Cattle

Bhuiyan, M.S.A.,Bhuiyan, A.K.F.H.,Yoon, D.H.,Jeon, J.T.,Park, C.S.,Lee, J.H. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.10

To determine the origin and genetic diversity of Red Chittagong (RC) cattle in Bangladesh, we analyzed mitochondrial DNA displacement loop (D-loop) sequences of 48 samples along with 22 previously published sequences from Bos indicus and Bos taurus breeds. Twenty five haplotypes were identified in RC cattle that were defined by 44 polymorphic sites and nucleotide diversity was $0.0055{\pm}0.0026$. The estimated sequence divergence times between RC and other zebu cattle breeds studied ranged between 22,700-26,900 years before present (YBP) which, it is suggested, predate domestication of RC cattle. Furthermore, it is assumed that introgressions have occurred in this breed mainly from Indian zebu breeds in the recent millennia. The phylogenetic studies showed RC cattle clustered with Bos indicus lineage with two distinct haplogroups representing high genetic variability of this breed. These findings can be used for designing proper breeding and conservation strategies for RC cattle in Bangladesh.

Estimates of genetic parameters for fatty acid compositions in the <i>longissimus dorsi</i> muscle of Hanwoo cattle

Bhuiyan, M. S. A.,Lee, D. H.,Kim, H. J.,Lee, S. H.,Cho, S. H.,Yang, B. S.,Kim, S. D.,Lee, S. H. Cambridge University Press 2018 Animal Vol.12 No.4

<P>We estimated the heritabilities (<I>h</I><SUP>2</SUP>) and genetic and phenotypic correlations among individual and groups of fatty acids, as well as their correlations with six important carcass and meat-quality traits in Korean Hanwoo cattle. Meat samples were collected from the <I>longissimus dorsi</I> muscles of 1000 Hanwoo steers that were 30-month-old (progeny of 85 proven Hanwoo bulls) to determine intramuscular fatty acid profiles. Phenotypic data on carcass weight (CWT), eye muscle area (EMA), back fat thickness (BFT), marbling score (MS), Warner-Bratzler shear force (WBSF) and intramuscular fat content (IMF) were also investigated using this half-sib population. Variance and covari.ance components were estimated using restricted maximum likelihood procedures under univariate and pairwise bivariate animal models. Oleic acid (C18:1n-9) was the most abundant fatty acid, accounting for 50.69% of all investigated fatty acids, followed by palmitic (C16:0; 27.33%) and stearic acid (C18:0; 10.96%). The contents of saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs) were 41.64%, 56.24% and 2.10%, respectively, and the MUFA/SFA ratio, PUFA/SFA ratio, desaturation index (DI) and elongation index (EI) were 1.36, 0.05, 0.59 and 0.66, respectively. The <I>h</I><SUP>2</SUP> estimates for individual fatty acids ranged from very low to high (0.03±0.14 to 0.63±0.14). The <I>h</I><SUP>2</SUP> estimates for SFAs, MUFAs, PUFAs, DI and EI were 0.53±0.14, 0.49±0.14, 0.23±0.10, 0.51±0.13 and 0.53±0.13, respectively. The genetic and phenotypic correlations among individual fatty acids and fatty acid classes varied widely (−0.99 to 0.99). Notably, C18:1n-9 had favourable (negative) genetic correlations with two detrimental fatty acids, C14:0 (−0.76) and C16:0 (−0.92). Genetic correlations of individual and group fatty acids with CWT, EMA, BFT, MS, WBSF and IMF ranged from low to moderate (both positive and negative) with the exception of low-concentration PUFAs. Low or near-zero phenotypic correlations reflected potential non-genetic contributions. This study provides insights on genetic variability and correlations among intramuscular fatty acids as well as correlations between fatty acids and carcass and meat-quality traits, which could be used in Hanwoo breeding programmes to improve fatty acid compositions in meat.</P>

Radix Puerariae modulates glutamatergic synaptic architecture and potentiates functional synaptic plasticity in primary hippocampal neurons

Bhuiyan, M.M.,Haque, Md.N.,Mohibbullah, Md.,Kim, Y.K.,Moon, I.S. Elsevier Ireland Ltd 2017 Journal of Ethnopharmacology Vol.209 No.-

Ethnopharmacological relevance: Neurologic disorders are frequently characterized by synaptic pathology, including abnormal density and morphology of dendritic spines, synapse loss, and aberrant synaptic signaling and plasticity. Therefore, to promote and/or protect synapses by the use of natural molecules capable of modulating neurodevelopmental events, such as, spinogenesis and synaptic plasticity, could offer a preventive and curative strategy for nervous disorders associated with synaptic pathology. Radix Puerariae, the root of Pueraria monatana var. lobata (Willd.) Sanjappa&Pradeep, is a Chinese ethnomedicine, traditionally used for the treatment of memory-related nervous disorders including Alzheimer's disease. In the previous study, we showed that the ethanolic extracts of Radix Puerariae (RPE) and its prime constituent, puerarin induced neuritogenesis and synapse formation in cultured hippocampal neurons, and thus could improve memory functions. Aims of the study: In the present study, we specifically investigated the abilities of RPE and puerarin to improve memory-related brain disorders through modulating synaptic maturation and functional potentiation. Materials and methods: Rat embryonic (E19) brain neurons were cultured in the absence or presence of RPE or puerarin. At predetermined times, cells were live-stained with DiO or fixed and immunostained to visualize neuronal morphologies, or lysed for protein harvesting. Morphometric analyses of dendritic spines and synaptogenesis were performed using Image J software. Functional pre- and postsynaptic plasticity was measured by FM1-43 staining and whole-cell patch clamping, respectively. RPE or puerarin-mediated changes in actin-related protein 2 were assessed by Western blotting. Neuronal survivals were measured using propidium iodide exclusion assay. Results: RPE and puerarin both: (1) promoted a significant increase in the numbers, and maturation, of dendritic spines; (2) modulated the formation of glutamatergic synapses; (3) potentiated synaptic transmission by increasing the sizes of reserve vesicle pools at presynaptic terminals; (4) enhanced NMDA receptor-mediated postsynaptic currents, and (5) increased cell viability against naturally occurring cell death. Moreover, upregulation of actin-related protein 2 (ARP2) in RPE and puerarin treated brain neurons suggest that RPE and puerarin induced synaptic plasticity might be associated, at least in part, with ARP2-mediated actin-dependent regulation of spinogenesis. Conclusions: Our findings indicate that RPE and puerarin might play a substantial role in the morphological and functional maturation of brain neurons and suggest that RPE and puerarin are potentially valuable preventative therapeutics for memory-related nervous disorders.

Analysis of single nucleotide polymorphisms (SNPs) in bovine CSRP3 and caveolin gene family

Bhuiyan, M.S.A.,Yu, S.L.,Yoon, D.H.,Lee, J.H. 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

Anthocyanins Extracted from Black Soybean Seed Coat Protect Primary Cortical Neurons against in Vitro Ischemia

Bhuiyan, M.I.H.,Kim, J.Y.,Ha, T.J.,Kim, S.Y.,Cho, K.-O. PHARMACEUTICAL SOCIETY OF JAPAN 2012 Biological & pharmaceutical bulletin Vol.35 No.7

Genetic parameters of carcass and meat quality traits in different muscles (longissimus dorsi and semimembranosus) of Hanwoo (Korean cattle)

Bhuiyan, M. S. A.,Kim, H. J.,Lee, D. H.,Lee, S. H.,Cho, S. H.,Yang, B. S.,Kim, S. D.,Lee, S. H. American Society of Animal Science 2017 Journal of Animal Science Vol. No.

<P>We estimated heritability (h(2)) and genetic and phenotypic correlations for carcass and meat quality traits of longissimus dorsi (LD) and semi-membranosus (SM) muscles in 30-mo-old Hanwoo steers. Variance and covariance components were estimated using REML procedures under univariate and bivariate models. The mean carcass weight (CWT), eye muscle area (EMA), back fat thickness (BFT), and marbling score (MS) were 428.20 +/- 46.30 kg, 87.38 +/- 8.54 cm2, 13.00 +/- 5.14 mm, and 5.21 +/- 1.56, respectively. The mean CIE reflectance of meat lightness (L*), redness (a*), and yellowness (b*) were 40.01 +/- 2.73, 22.37 +/- 2.18, and 10.35 +/- 1.46, respectively, in LD muscles and 36.33 +/- 2.44, 22.91 +/- 2.43, and 10.25 +/- 1.65, respectively, in SM muscles. The mean Warner-Bratzler shear force (WBSF), intramuscular fat content (IMF), water-holding capacity (WHC), and protein and ash content in LD and SM muscles were 3.84 +/- 0.96 and 6.52 +/- 1.21 kg, 15.91 +/- 4.39 and 5.10 +/- 1.94%, 62.07 +/- 3.38 and 71.61 +/- 2.06%, 20.01 +/- 1.39 and 21.34 +/- 0.89%, and 0.80 +/- 0.10 and 0.93 +/- 0.07, respectively. The h(2) estimates of CWT, EMA, BFT, and MS were 0.51 +/- 0.13, 0.45 +/- 0.13, 0.29 +/- 0.09, and 0.22 +/- 0.08, respectively. The h(2) estimates were moderate for meat quality traits and were 0.37 +/- 0.12, 0.40 +/- 0.12, 0.33 +/- 0.10, 0.33 +/- 0.10, 0.30 +/- 0.11, and 0.24 +/- 0.09 for L*, WBSF, IMF, WHC, and protein and ash content, respectively, in LD muscle; estimates from SM muscle were comparatively low (0.08 +/- 0.06 to 0.25 +/- 0.09). Estimates of h(2) for a* and b* were also low (0.08 +/- 0.06 to 0.13 +/- 0.07). Carcass weight had a moderate, positive genetic correlation with EMA (0.60 +/- 0.13) and a weak correlation with MS and BFT. The genetic correlations among the 3 colorimeter variants were strong and positive within and between muscles. Intramuscular fat content had moderate to strong and negative genetic correlations with WBSF (-0.49 +/- 0.18), WHC (-0.99 +/- 0.01), and protein (-0.93 +/- 0.04) and ash content (-0.98 +/- 0.06) in LD muscle, whereas the associations were less pronounced in SM muscle. In general, CWT and EMA had low genetic and phenotypic correlations with meat quality traits, which suggests that the traits are independent and have distinct genetic contributions in each muscle. Conversely, with few exceptions, meat quality traits had genetic and phenotypic correlations with MS and BFT. In conclusion, the estimated genetic parameters for carcass and meat quality traits could be used for genetic evaluation and breeding programs in Korean Hanwoo cattle populations.</P>

Coloration of polyester fiber with natural dye henna (Lawsonia inermis L.) without using mordant: a new approach towards a cleaner production

M. A. Rahman Bhuiyan,A. Ali,A. Islam,MA Hannan,S. M. Fijul Kabir,M. N. Islam 한국의류학회 2018 Fashion and Textiles Vol.5 No.1

The demand of natural colorants for the dyeing of textile fbers has been increasing gradually in recent years due to a growing global ecological awareness as well as a greater emphasis on a cleaner and greener production process. The eco-friendly dyeing of polyester fber with natural dye henna is a novel approach that has extensively been studied in this article. To this end, the dyeing of polyester fber with henna dye was conducted at diferent temperatures without using hazardous metallic mordants. Then the dyeing performance was investigated in terms of depth of shade measurement, analysis of colorimetric properties of color and assessment of color fastness properties of henna dyed polyester fabric samples. The amount of dye absorption by fber and the resulting depth of shade were found to increase with increasing dyeing temperature. In case of colorfastness properties, all dyed substrates demonstrated excellent fastness ratings against washing, rubbing and perspiration with little to no deterioration of color. Moreover, the detailed morphological study revealed that surface structure of fber remained unchanged after dyeing at an elevated temperature and pressure. So, the fndings suggest that dyeing of polyester fber with henna dye without any metallic mordants is a promising approach to get the desired dyeing performance alongside opening up an avenue for eco-friendly dyeing.

DNA Polymorphisms in SREBF1 and FASN Genes Affect Fatty Acid Composition in Korean Cattle (Hanwoo)

Bhuiyan, M.S.A.,Yu, S.L.,Jeon, J.T.,Yoon, D.,Cho, Y.M.,Park, E.W.,Kim, N.K.,Kim, K.S.,Lee, J.H. Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.6

Sterol regulatory element binding factor 1 (SREBF1) and fatty acid synthase (FASN) genes play an important role in the biosynthesis of fatty acids and cholesterol, and in lipid metabolism. This study used polymorphisms in the intron 5 of bovine SREBF1 and in the thioesterase (TE) domain of FASN genes to evaluate their associations with beef fatty acid composition. A previously identified 84-bp indel (L: insertion/long type and S: deletion/short type) of the SREBF1 gene in Korean cattle had significant associations with the concentration of stearic (C18:0), linoleic (C18:2) and polyunsaturated fatty acids (PUFA). The stearic acid concentration was 6.30% lower in the SS than the LL genotype (p<0.05), but the linoleic and PUFA contents were 11.06% and 12.20% higher in SS compared to LL (p<0.05). Based on the sequence analysis, five single nucleotide polymorphisms (SNPs) g.17924G>A, g.18043C>T, g.18440G>A, g.18529G>A and g.18663C>T in the TE domain of the FASN gene were identified among the different cattle breeds studied. Among these, only g.17924 G>A and g.18663C>T SNPs were segregating in the Hanwoo population. The g.17924G>A SNP is a non-synonymous mutation (thr2264ala) and was significantly associated with the contents of palmitic (C16:0) and oleic acid (C18:1). The oleic acid concentration was 3.18% and 2.79% higher in Hanwoo with the GG genotype than the AA and AG genotypes, respectively (p<0.05), whereas the GG genotype had 3.8% and 4.01% lower palmitic acid than in those cattle with genotype AA and AG, respectively (p<0.05). Tissue expression data showed that SREBFI and FASN genes were expressed in a variety of tissues though they were expressed preferentially in different muscle tissues. In conclusion, the 84-bp indel of SREBF1 and g.17924G>A SNP of the FASN gene can be used as DNA markers to select Hanwoo breeding stock for fatty acid composition.

Energy Value of Cassava Products in Broiler Chicken Diets with or without Enzyme Supplementation

M.M. Bhuiyan,P.A. Iji 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.9

This study investigated the metabolizable energy (ME) intake, net energy of production (NEp), heat production (HP), efficiencies of ME use for energy, lipid and protein retention as well as the performance of broiler chickens fed diets based on cassava chips or pellets with or without supplementation with an enzyme product containing xylanase, amylase, protease and phytase. The two products, cassava chips and pellets, were analysed for nutrient composition prior to feed formulation. The cassava chips and pellets contained 2.2% and 2.1% crude protein; 1.2% and 1.5% crude fat; and 75.1% and 67.8% starch, respectively. Lysine and methionine were 0.077%, 0.075%, and 0.017%, 0.020% protein material, respectively, while calculated ME was 12.6 and 11.7 MJ/kg, respectively. Feed intake to day 21 was lower (p<0.01) on the diet containing cassava chips compared to diets with cassava pellets. Enzyme supplementation increased (p<0.01) feed intake on all diets. Live weight at day 21 was significantly (p<0.01) reduced on the diet based on cassava chips compared to pellets, but an improvement (p<0.01) was noticed with the enzyme supplementation. Metabolizable energy intake was reduced (p<0.01) by both cassava chips and pellets, but was increased (p<0.01) on all diets by enzyme supplementation. The NEp was higher (p<0.01) in the maize-based diets than the diets containing cassava. Enzyme supplementation improved (p<0.01) NEp in all the diets. Heat production was highest (p<0.01) on diets containing cassava pellets than on cassava chips. It is possible to use cassava pellets in diets for broiler chickens at a level close to 50% of the diet to reduce cost of production, and the nutritive value of such diets can be improved through supplementation of enzyme products containing carbohydrases, protease, and phytase.

Energy Value of Cassava Products in Broiler Chicken Diets with or without Enzyme Supplementation

Bhuiyan, M.M.,Iji, P.A. Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.9

This study investigated the metabolizable energy (ME) intake, net energy of production (NEp), heat production (HP), efficiencies of ME use for energy, lipid and protein retention as well as the performance of broiler chickens fed diets based on cassava chips or pellets with or without supplementation with an enzyme product containing xylanase, amylase, protease and phytase. The two products, cassava chips and pellets, were analysed for nutrient composition prior to feed formulation. The cassava chips and pellets contained 2.2% and 2.1% crude protein; 1.2% and 1.5% crude fat; and 75.1% and 67.8% starch, respectively. Lysine and methionine were 0.077%, 0.075%, and 0.017%, 0.020% protein material, respectively, while calculated ME was 12.6 and 11.7 MJ/kg, respectively. Feed intake to day 21 was lower (p<0.01) on the diet containing cassava chips compared to diets with cassava pellets. Enzyme supplementation increased (p<0.01) feed intake on all diets. Live weight at day 21 was significantly (p<0.01) reduced on the diet based on cassava chips compared to pellets, but an improvement (p<0.01) was noticed with the enzyme supplementation. Metabolizable energy intake was reduced (p<0.01) by both cassava chips and pellets, but was increased (p<0.01) on all diets by enzyme supplementation. The NEp was higher (p<0.01) in the maize-based diets than the diets containing cassava. Enzyme supplementation improved (p<0.01) NEp in all the diets. Heat production was highest (p<0.01) on diets containing cassava pellets than on cassava chips. It is possible to use cassava pellets in diets for broiler chickens at a level close to 50% of the diet to reduce cost of production, and the nutritive value of such diets can be improved through supplementation of enzyme products containing carbohydrases, protease, and phytase.