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심수진 ( Su-jin Sim ),이소라 ( Sora Lee ),이형원 ( Hyung Won Lee ),김석주 ( Seokju Kim ) 한국목재공학회 2022 한국목재공학회 학술발표논문집 Vol.2022 No.2
The present study aimed to investigate comparative anti-inflammatory effects of Litsea japonica. Bioactive constituents were isolated and identified by column chromatography, thin layer chromatography UV and NMR. Five useful compounds were separated from the leaves of the L.japonica.
꾸지뽕나무 열매 isoflavonoid 화합물에 대한 정량분석법 개발
윤순영 ( Sun Young Yoon ),김석주 ( Seok Ju Kim ),심수진 ( Su Jin Sim ),이학주 ( Hak-ju Lee ) 한국목재공학회 2016 목재공학 Vol.44 No.3
본 연구는 꾸지뽕나무 열매에서 분리 및 정제한 isoflavonoid 성분에 대하여 Ultra Performance Liquid Chromatography(UPLC)을 이용하여 분석법을 개발하고 검증하고자 하였다. 꾸지뽕나무 열매를 methanol로 추출하여 n-hexane, ethylacetate, 물 순으로 분액 후, ethyl acetate 추출물을 silica gel 컬럼과 sephadex LH-20 컬럼을 사용하여 4종의 isoflavonoid들을 분리하였다. 분리한 4종의 isoflavonoid들은 기기분석(UV-Vis spectroscopy, ESI-MS, 1H NMR, 13CNMR)을 통하여 alpinumisoflavone, 6,8-diprenyl orobol, 6,8-diprenyl genistein, 4.-O-methylalpinumisoflavone으로 확인 및 동정하였다. 이 성분들을 2% acetic acid 용액(용매 A)과 2% acetic acid가 함유된 MeOH 용액(용매 B)을 기울기이동상으로 하여 C18 컬럼이 장착된 UPLC로 분석하였다. 분석 조건은 ICH (International Conference on Harmonisation)가이드라인에 기술된 선택성, 직선성, 정량한계, 검출한계, 정확성 및 정밀성을 측정하여 분석법의 타당성을 검증하였다. 또한 검증한 분석방법을 이용하여 꾸지뽕나무 열매의 채취시기별 함량을 조사하였다. 그 결과, 미숙과는 7월과 8월에 각 성분의 함량이 증가하였다가 9월에는 감소하였고, 성숙과의 경우 9월 미숙과 보다 각 성분들이 전반적으로 많이 함유되어 있었다. In this study, an analytical method to evaluate the quality of isoflavonoid compounds purified and isolated from the fruits of Cudrania tricuspidata was developed and validated using Ultra Performance Liquid Chromatography (UPLC). The fruits of C. tricuspidata were extracted with methanol and further fractioned with n-hexane, ethyl acetate and water. The resulting ethyl acetate extract separated into four isoflavonoid compounds by a combination of silica gel and sephadex LH-20 column chromatography. The structures of the compounds were elucidated as alpinumisoflavone, 6,8-diprenyl genistein, 6,8-diprenyl orobol, 4.-O-methylalpinumisoflavone by various techniques such as UV-Vis, ESI-MS, 1H NMR and 13C NMR spectroscopy. Finally, a method to characterize the compounds was developed by using the UPLC equipped with a C18 column and a gradient mobile phase system consisting of 2% acetic acid in water (solvent A) and 2% acetic acid in methanol (solvent B). The developed method was validated with the parameters such as selectivity, linearity, limit of detection, limit of quantitation, accuracy, and precision, which are defined by the ICH (International Conference on Harmonization). Using the validated method, the compounds in the fruits harvested in different months were also quantitatively analyzed. We propose this approach this approach can readily be utilized as an efficient evaluation method to quantify the extracts of C. tricuspidata.
UV/H₂O₂, UV/TiO₂ 시스템에서 염료의 색도 및 COD 제거
김계월(Kim Kei Woul),박정미(Park Joung Mi),심수진(Sim Su Jin),이희정(Yee Hi Joung),이동석(Rhee Dong Seok) 강원대학교 산업기술연구소 2000 産業技術硏究 Vol.20 No.1
The Photocatalytic decolorization and degradation of commercial dyes were studied using a batch reactor. Degussa P25 titanium dioxide and H₂O₂ were used as the photocatalyst and proved to be effective for dyes degradation when they were irradiated with UV light. The light source was a 20W low pressure mercury lamp. Three different kinds of dyes, such as direct dye(congo red), acid dye(acid black) and disperse dye(disperse blue) were tested. Extending the UV only treatment up to 120min, direct dye was decolorized to 60% and degraded to 30% as COD. On the other side, acid and disperse dyes were eliminated less than 10% as color and COD. But, color and COD were eliminated about 90% for all of the three dyes by UV/H₂O₂ system. And then the most effective decolorization was done for direct dye with 96% removal efficiency by UV/TiO₂ system at 120min 500mg/L of TiO₂.
UV/H_2O_2, UV/TiO_2 시스템에서 염료의 색도 및 COD 제거
김계월,박정미,심수진,이희정,이동석 강원대학교 산업기술연구소 2000 産業技術硏究 Vol.20 No.A
The Photocatalytic decolorization and degradation of commercial dyes were studied using a batch reactor. Degussa P25 titanium dioxide and H_2O_2 were used as the photocatalyst and proved to be effective for dyes degradation when they were irradiated with UV light. The light source was a 20W low pressure mercury lamp. Three different kinds of dyes, such as direct dye(congo red), acid dye(acid black) and disperse dye(disperse blue) were tested. Extending the UV only treatment up to 120min, direct dye was decolorized to 60% and degraded to 30% as COD. On the other side, acid and disperse dyes were eliminated less than 10% as color and COD. But, color and COD were eliminated about 90% for all of the three dyes by UV/H_2O_2 system. And then the most effective decolorization was done for direct dye with 96% removal efficiency by UV/TiO_2 system at 120min 500mg/L of TiO_2.