컨버전스 미디어를 위한 Trans-Generation GUI 디자인에 대한 연구 -경험에 의한 디자인을 중심으로-

정민 ( Min Chung ),정준용 ( Joon Yong Jung ) 한국기초조형학회 2009 기초조형학연구 Vol.10 No.1

Variation of antigenic proteins of eggs and developmental stages of Paragonimus westermani

Yoon KONG(공윤),Joon-Yong CHUNG(정준용),Doo-Hee YUN(윤두희),Lee-Su KIM(김이수),Shin-Yong KANG(강신영),Akira ITO,Liang MA,Seung-Yull CHO(조승열) 대한기생충학열대의학회 1997 The Korean Journal of Parasitology Vol.35 No.3

한국인에서 Lipoprotein lipase gene polymorphism의 빈도와 혈관질환과의 관계

정준용,박소영,김제,성인환,김영건,전은석 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

Background : Lipoprotein lipase(LPL) is a key enzyme in the metabolism of serum triglycerides(TG) which is utilized in the peripheral tissue as free fatty acid and stored in adipose tissue. LPL gene is consisted of 10 exons which encode 475 amino acids and over 9 LPL gene polymorphisms have been reported. It's polymorphisms are related to the lipid levels and the severity of atherosclerosis in the coronary artery disease. In Korea, LPL polymorphisms has not been reported yet. This study is purposed on the incidences of LPL gene mutations and on it's relationships with blood lipid levels and the severity of atherosclerosis. Methods : Our subjects were consisted of three groups, normal controls(n=50), coronary artery disease(CAD, n=51) and cerebrovascular disease(CVD, n=52). The PCR amplified genomic DNA from peripheral white blood cell were analyzed with restriction fragment length polymorphisms (RFLP by two different restriction enzyme(PVU II, Hind III). Results : Total cholesterol(TC) were higher in CVD than in controls and in CAD (203±60mg/ dl vs 188±37, 167±42, p<0.01). Triglyceride(TG) were also elevated in CAD(166±65mg/dl vs 122±62 in controls, p<0.05). HDL cholesterol(HDL-C) were higher in controls than in CVD and CAD(49±9mg/dl vs 36±10, 44±9, p<0.05). The incidences of Hind III RFLP and PVU II RFLP has no differences among groups. Only HDL-C was elevated in Hind III RFLP(-/-) homozygotes. Conclusions : The LPL gene mutations in intron 6 and 8 have no direct effects on the lipid profiles and the severity of coronary artery disease. Although LPL is a key enzyme in TG metabolism, two mutations in this study could not change the activity of LPL, nor were not a marker of linked other site mutations. The mutation(s) in exon which encode amino acid for enzyme activity should be detected to analyze the pathophysiology of the atherogenesis.

Real-Time RT-PCR 기법을 이용한 사이토카인 유전자 발현의 정량분석

정준용 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.2

The analysis of cytokine profiles plays a central part in the characterization of disease-related inflammatory pathways and the identification of functional properties of immune cells, both for research and for clinical diagnosis. Because tissue biopsy samples are too small to allow the detection of cytokine protein, the detection of mRNA expression level by reverse transcription-polymerase chain reaction (RT-PCR) analysis is often used to investigate the cytokine profiles in inflammatory lesions. RT-PCR itself is a qualitative method, indicating the presence or absence of specific mRNA. The most widely accepted method is qualitative competitive RT-PCR, based on internal shortened standards. Recently, real-time RT-PCR has been introduced for quantitative analysis of cytokine expression profiles. The real-time RT-PCR is becoming widely used to quantify cytokines from cells, tissues, tissue biopsies or body fluids. Being a very powerful and sensitive method it can be used to quantify mRNA expression levels of cytokines, which are often very low in the tissues under investigation. The method allows for the direct detection of PCR product during the exponential phases of the reaction, combining amplification and detection in one single step. In the review, I discussed the principle of real-time RT-PCR, the different methodologies and chemistries available, the assets, and some of pitfalls. Also, validation for a large panel of human cytokines and other factors playing a role in the immune system is discussed in detail. In summary, the real-time RT-PCR technique is very accurate and sensitive, allows a high throughput, and can be performed on very small samples.

국내 말라리아 상황과 연구동향

고원규,정준용 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.1S

Vivax malaria has re-emerged in the northern part of the Republic of Korea from 1993 after over 10 years absence. The number of cases has increased every year and reached to about 3000-4000 a year. Thanks to this epidemic researches and developments on malaria is activated in the country. Some important achievements produced by Institute of Malariology. Inje University are introduced. The geographical distributions of patients and antibody titers showed the characteristic of border malaria. Thus collaborative efforts with North Korea are necessary to control this epidemic successfully. In this epidemic, two different genotypes were found In the genes of CSP, DBP, MSP-1 (ICB 4-5), and AMA-1. The DNA structures of the genes were similar to them from East Asia. 58% of 80 patients' blood shows single genotype in each gene had a combination of genotypes (CSP SK-A:DBP SK-I:MSP-1), and 29% of them has another (CSP SK-B:DBP SK-Ⅱ:MSP-2). Thus it was concluded that genetically different two strains (KOR1 and KOR2) of P. vivax were mainly distributed in the endemic area. One-shot multiplex PCR was designed for differential diagnosis of two major species of Plasmodium by targeting the 18s rRNA gene. In an evaluation on 210 clinical samples the new PCR test showed very high feasibility, sensitivity (100%), and specificity (100%). Malaria is one of major infectious diseases in the world, causing 300-500 million clinical cases and more than one million deaths each year. The importance of vaccine and new drugs for malaria must be stressed.

동맥경화에서 클라미디아 항체의 양성률 및 그 의의

김준경,정준용,성보영,김윤철,김정희,이민수,성인환,전은석 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.2

식도 정맥류 출혈에 대한 내시경적 결찰요법의 치료효과

송진호,장윤식,이옥주,정준용,이연재,이상혁,설상영,정정명 인제대학교 1996 仁濟醫學 Vol.17 No.4

식도 정맥류 출혈은 사망율이 높은 치명적인 질환으로 현재까지 확실한 치료법은 없는 실정이다. 최근 식도 정맥류 출혈에 비교적 지혈률이 높고 부작용이 적은 방법으로 식도 정맥류 결찰술이 보고되었다. 1993년 2월부터 1995년 12월까지 급성 식도 정맥류 출혈로 인제대학 부속 부산백병원에 입원한 환자 중에 내시경적 결찰요법을 72예에서 시행하여 그 결과를 분석해 본 결과 부작용이 적고 지혈효과가 비교적 우수한 치료법이라고 판단되어 이를 보고하는 바이다. Endoscopic injection sclerotherapy(EIS) has been an effective method of treatment for bleeding esophageal varices. However, it is associated with some undesirable complications. Endoscopic variceal ligation(EVL) is a recently developed technique that eradicates esophageal varices and controls active bleeding with similar efficacy and less complication rates than EIS. We have performed EVL in 72 patients who bled from esophageal varices. Total 350 variceal lagations were performed during 104 separate sessions. Control rate of acute bleeding was 93.1% (67 of 72 patients) and five patients died after EVL due to failure of bleeding control. Rebleeding following initiation of EVL occurred in eight patients. Among them, bleeding was successfully controlled by EVL in five, and two patients died of uncontrollable bleeding. In one patient, bleeding was controlled but varices were not completely eradicated. Varices were eradicated or reduced to Grade I in 36 (53.7%) of 67patients by 1-9 ligations (mean 4.9) in 1-4 EVL sessions(mean 1.4). After EVL, there were several minor complications; mild substernal discomfort in seven patients, mild dysphagia in three and fever in two. These results suggest that EVL is a safe and effective method of treatment for bleeding esophageal varices, and eradication of esophageal varices can be obtained with less complications.

대동맥판폐쇄부전으로 나타난 Supraaortic Ridge와 Subaortic Membrane의 증례

류정곤,김윤철,성보영,김준경,정준용,성인환,전은석,나명훈,이영 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.8

대장용종증 동반이 없는 위에서 발견된 과오종 4예

이상혁,정정명,설상영,이연재,정준용,윤혜경,하희근,황희승 대한소화기내시경학회 1996 Clinical Endoscopy Vol.16 No.6

Hamartoma is a very rare congenital tumor. A few cases of hamartomatous pol yps in the stomach were reported. Most of harrurtomatous polyps in the stomach occur in patients of polyposis coli. We have experienced four cases of gastric hamartomatous polyps without polyposis coli. There are some differences between previously reported cases and ours. In our casee, the distribution of age is from fourth to ninth decade, men and women ratio is 1: 1. The number of polyps are one in three cases and two in one case. The size of polyps is from 5mm to 12 mm. The location of polyps are various, but most common in antrum. We have experienced four cases which were different from previously reported ones and report them with a brief review of literatures.

식도 정맥류 출혈에 대한 내시경적 결찰요법의 치료효과

이상혁,정정명,설상영,옥승철,정준용,조길현 대한소화기내시경학회 1995 Clinical Endoscopy Vol.15 No.1

Endoscopic injection sclerotheraphy(EIS) was an effective method for treatment of bleeding esophageal varices. However, it might be associated with some undesirable complications. Endoscopic variceal ligation(EVL) is a recently developed method for control of active variceal bleeding and eradicating esophageal varices with similar efficacy and minimal risk of complications compare to EIS. We performed EVL in 40 patients who had recently bled from esophageal varices. Total 198 variceal ligations were performed during 64 separate EVL session. Control rate of acute bleeding was 90%(36 of 40patients) and 4 patients died after EVL because of failure of bleeding control. Rebleeding following initiation of EVL occured in 5 patients-three patients were successfully controlled by EVL, one patient was controlled but eradication was impossible and one patient died. Varices were eradicated or reduced to grade I in 31(86.1% ) of 36 survivors by 1-9 ligation(mean 5.0) in l-4 EVL sessions(mean 1.6). After EVL, there were mild complications-mild substernal discomfort in 4 patients, mild dysphagia in 2 patients and fever in 2 patients. These results suggest that EVL is a safe and effective method for treatment of bleeding and eradication of esophageal varices with less complication.