해양플랜트용 Heave Compensator의 진동특성 평가에 관한 연구

황성구(Sung-gu Hwang),김귀남(Gwi-nam Kim),현장환(Jang-hwan Hyeon),정용길(Yong-gil Jung),허선철(Sun-chul Huh) 대한기계학회 2014 대한기계학회 춘추학술대회 Vol.2014 No.5

Offshore heave compensator is a structure that bears dynamic load (environmental load) such as ocean wave and wind, and influenced by vibration during operation. However, While the natural frequency of the structure is close to the dynamic load frequency, it can load to resonance phenomena. This study is composed of 3 model (upper position, middle position, lower position) depend on positions of crown block to vibrate characteristic of offshore heave compensator. Modal analysis and harmonic analysis fulfill vibration characteristic evaluation of finite element model by ANSYS Workbench V13, to judge stability of the structure.

폐 상피세포에서 PI3K/Akt 경로가 IκB/NF-κB 경로의 활성화에 미치는 영향

이상민 ( Lee Sang Min ),김윤경 ( Kim Yun Gyeong ),황윤하 ( Hwang Yun Ha ),이창훈 ( Lee Chang Hun ),이희석 ( Lee Hui Seog ),이춘택 ( Lee Chun Taeg ),김영환 ( Kim Yeong Hwan ),한성구 ( Han Seong Gu ),심영수 ( Sim Yeong Su ),유철규 ( 대한결핵 및 호흡기학회 2003 Tuberculosis and Respiratory Diseases Vol.54 No.5

국내 탐지견의 환경 및 위생 관리 현황 조사

이은채,정하정,황성구,이상락,Lee, Eun-Chae,Jeong, Ha-Jeong,Hwang, Seong-Gu,Lee, Sang-Rak 한국축산환경학회 2014 한국축산시설환경학회지 Vol.20 No.4

This research was conducted to grasp the present condition of environmental management relating to Korea detector dogs that performed their duties such as detection and lifesaving. We directly conducted a survey throughout visit to five government agencies. In environmental management case, average length and width of kennels were 1.78 m and 3.50 m, respectively. Types of floor were searched as coating cement, tile, ucrete and concrete. Most of government agencies ventilated kennels using ventilation fan systems with natural ventilation. But there were differences between government agencies in frequencies of the sanitary management items. On the other side, major diseases of the German shepherd breed as a major detector dog were in order of arthritis, measles, earache, and dermatosis. And their daily training time was average 4 hours. Finally, it is thought that these results could be used as a basic data of standard breeding management manual of Korea detector dog.

김치로부터 분리한 Lactobacillus plantarum JK-01의 동정 및 생리적 특성

조진국,이관호,조성진,윤여창,황성구,허강칠,최일신,Cho, Jin-Koo,Li, Guan-Hao,Cho, Sung-Jin,Yoon, Yoh-Chang,Hwang, Seong-Gu,Heo, Kang-Chil,Choe, Il-Shin 한국축산식품학회 2007 한국축산식품학회지 Vol.27 No.3

김치의 L. plantarum의 생균제적 특성을 조사하기 위하여 김치에서 산생산과 성장능력 등이 우수한 25종의 Lactobacillus sp.를 분리하였고, API kit 분석에 의하여 L. plantarum을 동정하고 재차 16S rDNA 염기서열(99.9% 상동성)을 비교한 후 L. plantarum JK-01로 표기하였다. L. plantarum JK-01은 MRS broth에서 배양시 18시간 후 $2.9{\times}10^{10}CFU/ml$로 최대로 증식하는 빠른 성장특성을 나타냈으며 pH도 4.5로 조속히 하강하였다. 효소활성은 xylanase, amylase, protease, phytase의 순으로 높은 활성을 포함하고 있는 것으로 예측되었다. L. plantarum JK-01은 pH 2에서도 $1.36{\times}10^5CFU/mL$가 생존하였고, 1%의 담즙산에서도 약 $10^6CFU/mL$이상이 생존하여 내산성과 내담즙산성이 강한 것으로 나타났다. 또, $60^{\circ}C$에서도 $3.3{\times}10^3$ CFU/mL 정도로 생존하는 내열성이 있었으며, 대장균과 함께 배양시 18시간 후 대장균을 사멸시키는 것으로 나타났다. 이상의 결과로부터 분리한 L. plantarum JK-01은 생균제로서 충분히 이용가치가 있는 것으로 사료되었다. In order to identify probiotic microorganisms, 25 isolates of Lactobacillus sp. were selected from kimchi based on their growth rates, lactic acid production and salt tolerance. The isolate JK-01 was identified as Lactobacillus plantarum by the API kit and 16S rDNA analysis (99.9% of homology), and named as L. plantarum JK-01. The maximum number of L. plantarum JK-01 was reached at 18 hr fermentation in MRS broth and the pH gradually decreased to 4.5. L. plantarum JK-01 showed high enzyme activities for xylanase, amylase, protease, and phytase on MRS agar plates containing each substrate. L. plantarum JK-01 showed high resistance to acidic pH and bile salts, and grew well even at pH 2.0 and 1.0% bile salt. In particular, L. plantarum JK-01 showed high heat stability as shown by $3.3{\times}10^3$ CFU/mL at $60^{\circ}C$. The isolate showed remarkable antimicrobial activity against E. coli in MRS broth based on its disappearance after 18 hr and clear zone formation using a paper disk assay. These results suggest that L. plantarum JK-01 may be probiotic in nature.

해양플랜트용 수직 보상기의 유공압 시스템 해석

정용길(Yong-Gil Jung),황성구(Sung-Gu Hwang),김귀남(Gwi-Nam Kim),윤영환(Yung-Hwan Yoon),현장환(Jang-Hwan Hyun),허선철(Sun-Chul Huh) 한국동력기계공학회 2015 동력시스템공학회지 Vol.19 No.1

백하수오 추출물이 In vitro 반추위 발효성상 및 메탄가스 생성에 미치는 영향

양승학,임정수,김별,황옥화,조성백,최동윤,최석근,황성구,Yang, Seung-Hak,Lim, Joung-Soo,Kim, Byul,Hwang, Ok-Hwa,Cho, Sung-Back,Choi, Dong-Yoon,Choi, Seok-Geun,Hwang, Seong-Gu 한국축산환경학회 2013 한국축산시설환경학회지 Vol.19 No.2

The objective of this study is to investigate the effects of Cynanchum wilfordii (CW) on cell viability, anti-oxidant activity, volatile fatty acid (VFA) production and methane gas production. Collected rumen fluid incubated with CW powder (1% w/v) for 12 and 24 hours were analyzed for pH, VFAs and methane. Alamar blue assay showed no significant difference on the viability of 3T3-L1 and C2C12 cells treated with CW for 24 hours. TBARS data showed a dose dependent increase on the antioxidant activity of CW. VFAs increased in the CW-treated groups compared to the control group. In addition, propionate increased more than other VFAs by the treatment with CW. There was a significant decrease in methane gas production in batch culture treated with CW in 12hrs. In conclusion, it was suggested that Cynanchum wilfordii could manipulate rumen fermentation considered by increasing VFA production and inhibition of methanogenesis.

사료내 한방약제 추출물 첨가가 과밀사육환경 하에서의 육계의 성장과 면역세포 증식에 미치는 영향

최형석,김기현,박상훈,남인식,황성구,남기택,Choi, Hyoung-Seog,Kim, Ki-Hyun,Park, Sang-Hoon,Nam, In-Sik,Hwang, Seong-Gu,Nam, Ki-Taeg 한국유기농업학회 2010 韓國有機農業學會誌 Vol.18 No.3

본 시험은 한방약제 추출물 급여가 과밀사육 환경에서의 육계 (Ross broiler)의 생산성 및 면역세포 중식에 미치는 영향을 조사하기 위하여 실시하였다. 육계 수컷 240수를 저밀도 사육구와 고밀도 사육구로 나누어 각각 대조구와 처리구를 두었으며 저밀도 사육구는 pen 당 10수씩 고밀도 사육구는 pen당 30 수씩 임의 배치하였다. 저밀도와 고밀도의 대조구 사료에 한방약제 추출물 0.23%(AP 0.2%+ DFP 0.03%)를 첨가 급여였다. 사육밀도에 따른 평균 사료섭취량은 저밀도 사육구에 비하여 고밀도 사육구에서 유의하게 낮게 나타났으며(p<0.05), 한방약제 추출물 첨가에 따른 영향은 없었다. 사육밀도에 따른 평균 체중은 고밀도 사육구에서 저밀도 사육구보다 유의하게 낮게 나타났으나(p<0.05), 저밀도와 고밀도 사육구 모두에서 한방약제 첨가 처리구가 대조구에 비하여 통계적 유의차는 없었으나 일당증체량은 개선되는 경향을 나타내었다. 과밀사육에 의한 증체량 감소 및 사료섭취량 감소 현상은 생육 중기에 주로 나타난 결과 이며 후기에는 이러한 환경에 적응하여 회복되는 것으로 나타났다. 사료요구율은 한방약제 첨가 처리구가 고밀도 사육구 및 저밀도 사육구에서 유의한 차이는 없었으나 모두 개선되는 경향을 보여 주었다. 면역세포증식은 비장세포 종식도, LPS로 자극시킨 B세포 증식도와 ConA로 자극시킨 T세포 증식도 모두에서 한방약제 추출물 처리구에서 유의한 차이를 보이며 높은 증식도를 나타내었다(p<0.05). 결과적으로 고밀도환경하에서 한방약제 추출물 급여에 따라 육계의 면역력이 증가한 것으로 판단되며, 궁극적으로 육계의 질병예방 및 일부 생산성 향상에도 긍정적인 영향을 미칠 수 있을 것으로 판단된다. This experiment was conducted to investigate the effects of dietary supplementation of oriental herb extracts (Alpha $Plus^{(R)}$ (AP)+Dried Foxglove Powder (DFP)) on growth performance and proliferation of splenocytes in broiler chicken raised under low or high density stocking conditions. A total of 240 male broiler chicks were randomly divided into four treatment groups with three replicates composed of ten birds in low density stocking groups and thirty birds in high density stocking groups. The four treatment groups were as follows: Cl (low density without AP and DFP); T1 (low density with AP 0.2% and DFP 0.03%); C2 (high density without AP and DFP); T2 (high density with AP 0.2% and DFP 0.03%). The feed intake of the high density stocking group was significantly lower than that of low density stocking groups during overall experimental period (p<0.05). The body weight of high density stocking groups was lower than that of low density stocking groups (p<0.05). The decrease of feed intake and body weight gain in growing stage (7~25day) under the high density stocking condition seems to be recovered in final stage (26~34day). Meanwhile dietary supplementation of AP+DFP in low and high density stocking groups tended to have higher daily body weight gain than control groups. The proliferation of splenocytes in treatment groups was significantly higher than control groups (p<0.05). In conclusion, the result of the present study suggested that the oriental herb extracts (AP+DFP) could be used as an alternative feed additive resources to improve growth performance and immune activity in broiler chickens.

혼합미생물제가 육우 송아지의 체내환경에 미치는 영향

양승학,김현섭,조원모,김상범,조성백,박규현,최동윤,황성구,유용희,Yang, Seung Hak,Kim, Hyeon Shup,Cho, Won Mo,Kim, Sang Bum,Cho, Sung Back,Park, Kyu Hyun,Choi, Dong Yoon,Hwang, Sung Gu,Yoo, Yong Hee 한국축산환경학회 2012 축산시설환경학회지 Vol.18 No.suppl.

증체량은 모든 처리구(281.4~294.5 kg)가 대조구(271.4 kg) 보다 높았으나 유의적인 차이는 없었다(P>0.05). PS구를 포함한 모든 처리구의 DM, CP 및 TDN 함량이 대조구에 비해 3~8% 향상되는 결과를 얻었으나 통계적인 차이는 없었다(P>0.05). 모든 시험축군에서 수의적 치료를 요하는 질병은 발생되지 않았다. ES구가 모든 처리구와 비교할 때 총 단백질량은 낮고 BUN은 높은 경향을 보였다(P>0.05). 면역물질인 IgG항체 농도는 전 처리구간에 유의적인 차이가 없었다(P>0.05). 혈중 glucose 농도는 대조구(114 mg/dl)를 제외한 모든 처리구(97.8~104 mg/dl)에서 낮은 경향을 보였다(P>0.05). ES구(109.8 mg/dl)의 총 cholesterol 농도가 대조구(86.3 mg/dl) 보다 26% 높았으나 유의적인 차이가 없었다(P>0.05). 간상피세포 손상여부와 관계있는 GOT, GPT는 대조구(56, 14IU/L)에 비해 모든 처리구(45~49, 11~13.8 IU/L)에서 낮은 수치를 보였으며 유의적인 차이는 없었다(P>0.05). 비타민 A는 PS구(36.1 IU/dl)가 다른 처리구(30.9~34.4 IU/dl) 보다 높았으나 유의적인 차이가 없었다(P>0.05). 비타민 C는 ES구(3 mg/L)가 다른 시험구(3.7~3.9 mg/L) 보다 낮았으나 유의적인 차이가 없었다(P>0.05). 또한 모든 처리구의 비타민 E(150.1~155.4 IU/dl)는 대조구(143.7 IU/dl) 보다 높았으나 유의적인 차이가 없었다(P>0.05). Effect of commercial Direct Fed Microbials (DFM) or protease treated feed (PTF) supplementation on growth rate and biogenic substances such as BUN, glucose, IgG, GOT, GPT and Vitamin A, C, E from Holstein steers was studied for 7 months. Thirty two steers aged 2~3 months were separated with 4 groups for control, DFM (PS), protease (ES) and their mix (PS + ES) supplementally fed 0, 100, 100 and 50 + 50 g/day respectively. Weight gain was averagely higher in PS than any others, although there were no differences significantly. All treatments enhanced to 3~8% of control in dry matter, crude protein and total digestible nutrient (P>0.05). Metabolic diseases with veterinary cure had not shown in this study. Plasma GOT and GPT were lower in the PS and ES than control. Plasma glucose concentration was also lower in PS than the others. Total cholesterol of ES was higher than the others but that of PS is the lowest. Plasma vitamin C was higher in PS than the others. It was shown that dietary PS affected change from glucose to vitamin C with not overloading liver. Conclusionally, PS and ES were shown to enhance metabolic health of steers during growing period.

TDABC에 의한 부산항 컨테이너터미널 원가분석과 유용성에 관한 연구

류동하(Dong Ha Ryu),안기명(Ki-Myung Ahn),황성구(Sung-Gu Hwang) 한국항만경제학회 2014 韓國港灣經濟學會誌 Vol.30 No.3

본 연구는 TDABC라는 새로운 접근 방법에 의해 컨테이너터미널에서 제공하는 항만하역 서비스에 대한 원가를 분석하여 터미널의 전략적 경영의사결정과 운영을 지원할 수 있는 방안을 제시하고자 하였다. 부산항의 H터미널 사례자료를 활용하여 기존의 개별원가계산방식과 ABC 및 TDABC 방식을 적용하였을 경우에 하역서비스별로 원가를 계산하여 TDABC에 의한 전략적 원가관리의 유용성을 살펴보았으며 개별원가계산방식과 전통적인 ABC에 의한 접근법의 한계를 극복하고 실무에서 검토할 수 있는 경영개선 방안을 제시하였다. 전통적 방법에 의한 개별원가분석은 한 컨테이너 터미널을 이용하는 다수의 고객 선사들의 작업조건과 물동량구성이 모두 상이함에도 불구하고 모든 고객선사의 원가가 동일하다고 가정하기 때문에 고객별 수익성 분석에 필요한 자료를 제공할 수 없다. 또한, 개별 고객과 터미널 하역요금 협상 시 원가에 대한 자료를 제공할 수 없으므로 형평성에 맞는 요율이나 또는 합리적 수순의 요율을 설계하는 과정을 지원하지 못하며, 하역요율이 원가 수준에 관계없이 터미널의 협상력과 수요공급 상 우월적 지위에 의하여 전적으로 결정되게 되는 단점이 있다. 그러나, TDABC 방식을 적용하였을 경우에는 미사용 시설원가분석이 가능하고 하역서비스별 및 프로세스별 수익성분석이 가능하여 적정하역요율 및 적정임대료 결정에 유용한 것으로 실증되었다. The purpose of the study is to suggest a new approach to the terminal operators in order for them to efficiently control their operation cost and support the strategic decision making system and build a performance evaluation process through the systematic cost analysis approach. This study implemented terminal cost analysis based on the traditional cost system, ABC and TDABC and compared the results of each approach. Throughout the study, the usefulness of TDABC was proved in finding operational problems and suggesting countermeasures for improving cost effectiveness and minimizing unused cost.

홍삼 부산물이 스트레스에 대한 산란계 생체반응에 미치는 영향

홍준기(Joon Ki Hong),봉미희(Mi Hee Bong),박준철(Jun Cheol Park),문홍길(Hong Kil Moon),이상철(Sang Cheul Lee),이준헌(Jun Heon Lee),황성구(Seong Gu Hwang) 韓國家禽學會 2012 韓國家禽學會誌 Vol.39 No.1

This study was conducted to determine the possible use of Red Ginseng marc as stress inhibiter in thermal stress (temperature humidity index 86) and lipopolysaccharide (LPS)-exposed laying hens by investigating their effects on laying performance, blood biochemical parameters, immunoglobulin concentration and serum superoxide dismutase (SOD) like ability. A total of forty-five 52-wk-old laying hens (ISA Brown) were divided into 3 treatment groups with 5 replicates of 3 birds in each group. NC (negative control, no immune substances), PC (positive control, β-glucan 25 ppm) and RGM (Red Ginseng Marc 3%) were added in feed with respective substance. Egg production in RGM was significantly increased in comparison with NC groups for 8 weeks (P<0.05). On blood biochemical parameters, effects of ambient temperature is definite by showing significant difference in aspartate aminotransferase and others (P<0.05), but RGM both before and after thermal stimulation have no significant difference in comparison with other groups. And for 3 weeks after thermal stimulation, laying performance was also not significantly different among treatments. Immunoglobulin M content and SOD like activities after challenge with LPS were higher in the RGM and PC than NC (P<0.05). In conclusion, although ineffective as inhibiter in thermal stress, dietary supplementation of Red Ginseng marc improved SOD like activity and immune system by regulating immunoglobulin content in laying hens. These findings have laid the foundation for future studies of immunomodulation in laying hens fed Red Ginseng Marc and of evaluation of heat stress inhibitor.