상처 치료용 하이드로겔에 적용을 위한 방부제의 세포독성평가

한설희 ( Seol Hee Han ),권희정 ( Hui Jeong Gwon ),임윤묵 ( Youn Mook Lim ),안성준 ( Sung Jun An ),윤민호 ( Min Ho Youn ),조선영 ( Sun Young Jo ),문은경 ( Eun Gyeng Mun ),노영창 ( Young Chang Nho ) 한국공업화학회 2009 응용화학 Vol.13 No.1

Phenoxyethanol과 Paraben류는 항균제로서 제약과 화장품, 식품 안에 공통적으로 사용된다. 우리는 상처 치료용 겔의 부패 방지를 목적으로 phenoxyethanol, methyl paraben, propyl paraben 그리고 이들의 혼합물 사용을 위해 유효한 농도를 결정하고자 세포 독성 실험을 수행하였다. 세포독성 실험은 상기의 방부제를 mouse fibroblast cell line(L929)에 농도별로 처리하여 세포의 생존률로써 확인하였다. 이 결과를 바탕으로 phenoxyethanol, methyl paraben, propyl paraben 그리고 이들의 혼합물을 처리시 E.coli와 S.aureus의 성장 억제 활성을 확인하여 상처 치료용 겔에 적용하고자 한다. Phenoxyethanol and Paraben derivatives are well known as preservative and used in cosmetics and pharmaceutics and foods. Therefore, an investigation was carried out to assess the effect on the cytotoxicity of those preservatives when the preservatives were subjected to a commercial sterilization procedure using gamma-irradiation. The cytotoxicity tests of these hydrogels were evaluated by cell viability of L929 (mouse fibroblast). As results, the preservatives have a good cell viability results.

방사선 조사에 의한 방부제가 함유된 하이드로겔의 항균 효과

한설희 ( Seol Hee Han ),권희정 ( Hui Jeong Gwon ),임윤묵 ( Youn Mook Lim ),안성준 ( Sung Jun An ),윤민호 ( Min Ho Youn ),김해경 ( Hae Kyoung Kim ),문은경 ( Eun Gyeng Mun ),노영창 ( Young Chang Nho ) 한국공업화학회 2008 응용화학 Vol.12 No.2

골프 프로-암에 참여하는 여자프로선수들의 인식

한설희(Han, Seol-Hee),김영재(Kim, Young-Jae) 한국체육과학회 2016 한국체육과학회지 Vol.25 No.3

In this study, we focused on identifying the awareness of female professional golfers participating in Pro-Am when they experience Pro-Am which is held before the golf tour by conducting in-depth interviews with golfers of the Korea Ladies Professional Association(KLPGA). To achieve the goals of this study, we conducted in-depth interviews with 10 female professional golfers with over five years of experience in their careers who have participated in Pro-Am at home and abroad more than 50 times. Golf Pro-Am is an event which is held the day before the golf tour is held and amateur golfers and that professional golfers play the game in a team. Pro-Am of the Korea Ladies Professional Association (KLPGA) started 4 events in 1990 and grew to 19 events in 2014 and the number of golf tours and Pro-Am has steadily increased. However, there are not enough studies on Pro-Am currently. In this study, we studied the awareness of female professional golfers participating Pro-Am. The research result indicated that female professional golfers have two different parts of awareness which are positive awareness and negative awareness on participating Pro-Am. It showed that positive awareness is the pride in participating Pro-Am and diverse opportunities of personal connections while negative awareness is physical burdens and ignorance about amateur golfers(VIP). In this study, there were limits on identifying what the experiences and awareness of female golfers on Pro-Am are even though we conducted in-depth interviews with female golfers. Therefore, we need to analyze the internally psychological factors and externally psychological factors in future studies.

한국 골프 도슨트 프로그램 적용 가능성에 대한 고찰

한설희(Han, Seol-Hee),김영재(Kim, Young-Jae) 한국체육과학회 2017 한국체육과학회지 Vol.26 No.4

In this study, an in-depth interview was conducted to hear about experience in participating in the golf docent program in detail. To achieve the study purpose, this study targeted 2 golf specialists out of 3 people including the researcher, who experienced the docent program at the President’s Cup in the Jack Nicklaus Golf Club of Songdo, Incheon on October 2015 and the following results were obtained. First, in relation to the title of the program, ‘Meet A Docent’, the two golf specialists replied that the docent program provided new experience, there were differences between golf commentary and the docent program, and the gallery showed satisfactory reaction. Second, in relation to the docent program as a new golf commentary, the gold specialists replied that it added realism to a golf commentary, the docent program provided a new event of golf, and qualifications for being a golf docent should be established. This study targeted two golf specialists with experience in working as docents for an in-depth interview. But the participants were limited and there was a limit in concrete analysis. Nevertheless, there were positive responses about the docent program in golf from the specialists and there should be continued studies on the golf docent program.

NIH3T3 세포에서 UVB에 의한 세포고사와 DNA 단사절단에 미치는 fisetin의 효과

정세진,김돈영,한설희,신상민,차재영,박노복,이정섭,박종군,Jeong, Se-Jin,Kim, Don-Young,Han, Seol-Hee,Shin, Sang-Min,Cha, Jae-Young,Park, Nou-Bog,Lee, Jung-Sup,Park, Jong-Kun 한국생명과학회 2007 생명과학회지 Vol.17 No.1

본 연구에서는 UVB에 조사된 NIH3T3 세포에서 세포고사와 DNA 단사절단에 미치는 fisetin후처리의 효과에 대해서 연구하였다. 세포에 UVB $(200J/m^2)$를 조사하고 정상배지에서 48시간 배양한 세포의 세포고사에 수반되는 핵분절은 50% 정도의 세포에서 관찰되었다. 흥미롭게도 배양배지에 fisetin이 첨가될 경우 핵분절을 보이는 세포의 빈도는 상당한 감소를 보였다. 알칼리 아가로스 겔에 의한 DNA 단사절단 분석에서 자외선 조사 후 fisetin처리는 정상배지 배양시보다 단사절단의 빈도를 감소시켜 DNA크기의 증가를 유도하였는데 이는 fisetin이 UVB에 의한 DNA 상해의 회복에 긍정적 효과를 나타냄을 시사한다 Western blot 분석에 의해 fisetin은 자외선 조사에 의해 활성화되는 p53의 수준을 유의한 수준으로 감소시키며 자외선 상해의 결과 세포주기의 정지에 수반되는 PCNA의 감소 경향을 다소 완화시켰다. 이러한 결과들은 fisetin이 DNA 회복의 활성을 통해 세포고사의 감소에 기여하며 이 과정에서 p53 및 PCNA의 수준변화와 관련하여 행동함을 시사한다. In the present study, we have investigated the effect of fisetin on the apoptosis and DNA single strand breaks in ultraviolet light B (UVB)-exposed NIH3T3 cells. Exposure of cells to UVB light $(200J/m^2)$ and post-incubation in growth medium for 48 hr resulted in about 50% of cells with apoptotic nuclear fragmentation. Addition of various concentrations of fisetin in the postincubation medium, however, significantly reduced the apoptotic nuclear fragmentation as compared with the values expected when the effects are additive and independent. DNA single strand breaks induced by UVB exposure were also significantly decreased by postincubation with fisetin. By Western blot analysis, fisetin post-incubation was shown to attenuate the p53 upregulation upon UVB exposure. Furthermore, the decrease of proliferating cell nuclear antigen (PCNA) level upon UVB exposure was alleviated by fisetin postincubation. These results suggest that fisetin decrease the apoptosis and increae DNA repair in a possible association with alteration of p53 and PCNA levels in UVB-exposed cells.

혈관평활근세포에서 산화에너지대사 억제에 의한 아밀로이드전구단백질 대사의 변화

한문구,최웅,김헌식,안희열,한설희 대한치매학회 2002 Dementia and Neurocognitive Disorders Vol.1 No.1

Background: A reduction in the activity of cytochrome c oxidase(COX) has been recently identified in mitochondria from platelets and postmortem brain tissue of AD patients Sodium azide (NaN₃). a COX inhibitor, is an effective chemical agent producing energy shortage and oxidative stress both in vitro and in vivo system Furthermore it has been suggested that vascular compromise could be either directly involved AD pathogenesis or indirectly associated with triggering pathogenetic events leading to AD This study was performed to investigate amyloid precursor protein (APP) metabolism by inhibition of mitochondrial energy metabolism in cultured vascular smooth muslce cells (VSMCs) Materials and Methods: VSMCs isolated from the aorta of seven weeks old Spraque-Dawley rat were treated with NaN₃in a low concentration (100-500μM) or in a high concentration (1-100mM) Cellular proliferation and viability were determined by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenly)-2H-tetrazolium) assay Cellular APP was detected with N-terminal specific antibody 22C11. Celldeath was determined by observation of morphology and terminal deoxynucleotidyl transferase-mediated dUTP-fluorescein nick end labeling stain (TUNEL) We used ginkgo biloba extract(EGb761) and melatonin as anti-oxidants to investigate the mechanism of latered APP metabolism Results: The viability of VSMCs was increased after treatment with 1 mM and 10mM NaN₃(p<0.05) unitl 3 hr and then dimnished Many TUNEL positive cells were found in 10mM and 100mM treatment group. but were not apoptotic in nature 22C11 immunoreactivity was not changed at 3 hr, 6hr, 12hr Anti-oxidants reduced cellular proliferation (p<0.05). but did not block TUNEL positivities and did not influence the 22C11 immunoreactivity In a low concentration NaN₃ treatment group the viability of VSMCs was increased concentration dependently(p<0.05) Immunoblot with 22C11 showed the concentration dependent decrease at 145 kDa, 125 kDa. and high molecular weight range (>160kDa) TUNEL staining showed DNA fragmentations and condensations of nuclear chromatin suggesting apoptosis After treatment with anti-oxidants, the cellular proliferation was more decreased (p<0.05), and TUNEL positive cell deaths were blocked Immunoreactivities of 125 kDa (immature APP). 145 kDa (mature APP). and higher molecular weight bands were recovered below 400μM of NaN₃ Immunoreactivity of 145 kDa was recovered in 100 μM NaN₃ treated group Conclusions: The presumed mechanism of low concentration COX inhibitor is the overproduction of reactive oxygen species resulting from a depression of the mitochondiral electron transport chain. whereas potential consequence of high concentration COX inhibitor might be related to decreassion of ATP synthesis and bioenergetic impairment Reactive oxygen radicals in response to low concentration COX inhibitor alter the processing of APP in VSMCs This investigation demonstrated analtered APP metabolism as a peripheral marker of AD Therefore VSMCs treated with low concentration COX inhibitor could be concsidered as a novel in vitro model of AD.

열공형과 비열공형 피질하 혈관성 치매에서 위험인자의 차이에 관한 비교 연구

배희준,정지향,유경호,나덕렬,김상윤,최경규,양동원,손의주,이상도,김재우,박경원,김응규,이재홍,박미영,한일우,함동석,최문성,하충건,최성혜,이애영,이병철,한설희 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.2

Backgrounds and Objectives: Vascular dementia is a group of dementing disoders arising from various stroke syndrome. Among these. subcortical ischemic vascular dementia (SIVD) is regarded as a relatively distinct clinical entity. However, MRI patterns of SIVD are not homogenous. In some patients, lacunes are dominant, and in others, subcortical white matter changes are. This study was designed to compare risk factor profiles between SIVD with and without multiple lacunes. Methods: We divided 47 subjects (22 males, mean age. 68 years) recruited from VADAPET (Multicenter Trial For Evaluation Of The Changes In the PET Images Of Subcortical Vascular Dementia Patient) study into two groups one with more than 5 lacunes in deep gray matter (lacune group) and the other with 5 or less(non-lacune group) Clinical characteristics and laboratory findings of two groups were compared. Results: Nineteen of 47 patients (40%) belonged to the lacune group. The lacune and non-lacune groups d d not differ in the following variables: age, hypertension, diabetes mellitus, hyperlipidemia heart disease, history of stroke or TIA, history of trauma or major surgery, family history of hypertension stroke, or dementia, age at diagnosis of dementia, body mass index, white blood cell count, ESR, CRP, fibrinogen, hemoglobin A1C, total cholesterol. LDL cholesterol creatinine, proteinuria, glucosuria, and microhematuria. However, male sex, smoking alcohol. hemoglobin, and HDL cholesterol were possibly associated more with lacune group SIVD than with non-lacune group (p<0 1) Multivariate analyses revealed that smoking, hemoglobin, and HDL cholesterol were independent predictors of SIVD with multiple lacunes Conclusion: Our study suggests that SIVD with multiple lacunes may be significantly different in smoking habits hemoglobin, and HDL cholesterol from SIVD without multiple lacunes.