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서정길(Jung-Kil Seo),김은정(Eun Jung Kim),박남규(Nam Gyu Park),김은희(Eun-Hi Kim),정준기(Joon-Ki Chung),황은영(Eun-Young Hwang),류홍수(Hong-Soo Ryu) 한국식품영양과학회 1999 한국식품영양과학회지 Vol.28 No.3
양식 해산어의 효율적인 수급과 소비 방법을 다양화하기 위하여 광어를 이용한 고음 추출물을 제조하여 이의 치료식으로서의 이용가능성을 타진한 결과는 다음과 같다. 광어 고음 추출물들은 rat의 십이지장에 대해 수축활성을 나타냈고, in vivo상태에서 혈압강하작용이 있었다. 또한 이들 추출물들은 rat의 대동맥에 대해 농도 의존적으로 이완활성을 보였다. RM 60 추출물은 MCF7 세포에 대해 항암효과를 나타냈지 만 다른 추출물들은 항암효과와 항균활성을 지니지 않았다. 광어 고음추출물들을 사료로 하여 rat의 혈액성상효과를 측정한 결과 대조군에 비해 HDL-콜레스테롤의 증가 이외에는 현저한 차이를 보이지 않았다. 따라서 이러한 결과들은 광어 고음 추출물에 다양한 생리활성물질들이 포함되어 있다는 것을 제시한다. The pharmacological effects of hydrocooked(110℃, 5 hours) extracts of Bastard halibut have been investigated. All of the hydrocooked extracts showed the measurable contractile effect on the isolated rat duodenum and decreased the normal blood pressure in anesthetized rat. The hydrocooked extracts also exhibited a dose-dependent relaxation on the isolated rat aorta precontracted with phenylephrine. Only RM 60 fraction of these extracts had the cytotoxic effect against MCF7 cell(human breast adenocarcinoma cell line), but the other fractions showed neither antibacterial activity nor antitumor activity. Although fish extracts fed group of rat maintained their original body weight, there were no notable changes in the hematological parameters, except that the levels of high-density lipoprotein was significantly increased. These results suggest that the hydrocooked extracts of bastard halibut may contain a variety of bioactive materials.
넙치 ( Paralichthys olivaceus ) 뇌로부터 phospholipase D 활성 억제 단백질의 정제 및 특성 규명
서정수(Jung Soo Seo),김은희(Eun Hi Kim),황은영(Eun Young Hwang),김남득(Nam Deuk Kim),김동선(Dong Sun Kim),이형호(Hyung Ho Lee),정준기(Joon Ki Chung) 한국수산과학회 2001 한국수산과학회지 Vol.34 No.4
본 연구에서는 넙치, Paralichthys olivaceus 뇌 조직에서 인지질가수분해효소 D (phospholipase D, PLD) 활성의 특성 규명 및 이 활성을 억제하는 단백질을 분리 정제하여 그 특성을 규명하였다. 넙치 뇌 조직에서 PLD 활성이 관찰되었으며, 이 활성은 포스파티딜 이노시톨 비스인산염 (phosphatidyl-inositol 4,5-bisphosphate, PIP₂)에 대해서 의존성을 나타내었으나, ADP-rebosylation factor (ARF)에 의해서는 영향을 받지 않았다. PLD 억제물은 넙치 뇌 조직의 세포질 분획물을 사용하여 여러 종류의 칼럼을 통하여 분리 정제하였고, 그 억제물의 분자크기 및 기작의 특성을 규명하였다. 마지막 chromatography을 통하여 여섯 개의 억제를 나타내는 분획물을 얻었으며. 이 중 두 개의 분획물인 IIA, IIB는 PIP₂-phosphatase activities를 나타내었다. 이 중 IIA 분획물은 면역화학적 분석을 통하여 inositolpolyphosphate 5-phosphatase family로 알려져 있는 신경말단 단백질인 synaptojanin으로 동정되었다. 그리고 IIB fraction은 Superose 12 gel filtration chromatography을 통하여 158-kDa의 크기로 확인되었으며, 이것은 면역화학적 분석을 통하여 synaptojanin과는 별개의 단백질로 판명되었다. 또한, IIB 분획물은 PIP₂phosphatase activity 확인 실험에서 대사산물로서 phosphatidylinositol phosphate (PIP)만을 생성하였다. 이 결과는 IIB분획물이 PIP₂의 4혹은 5 위치의 인산 (phosphate) 중 어느 하나만을 선택적으로 가수분해시킨다는 것을 암시한다. 이상의 연구 결과들을 종합하여 보면, 넙치 뇌 조직에는 다양한 형태의 PIP₂-phosphatases가 존재하며, 이들은 PIP₂-의존적인 PLD 활성의 억제조절과정에서 중요한 역할을 할 것으로 사료된다. Flounder brain cytosol contains protein inhibitors that markedly inhibit the activity of partially purified brain membrane phospholipase D (PLD) which is dependent on phosphatidylinositol 4,5-bisphosphate (PIP₂) but insensitive to ADP-ribosylation factor (ARF). The PLD inhibitors have been enriched through several chromatographic steps and characterized with respect to size and mechanism of inhibition. Sequential chromatography of the brain cytosol yielded six inhibitor fractions. Two (IIA and IIB) of six inhibitor fractions showed the PIP₂-phosphatase activities. IIA was identified as synaptojanin, a nerve terminal protein that has known to be a member of the inositolpolyphosphate 5-phosphatase family, by immunoblot analysis. IIB showed an apparent molecular mass of 158 kDa by Superose 12 gel filtration chromatography and was immunologically distinct from synaptojanin. IIB hydrolyzed PIP₂, yielding only phosphatidylinositol phosphate (PIP) as product, suggesting that IIB hydrolyzes only one phosphate from either the 4- or 5-position of PI (4,5)P₂. These studies demonstrate that the existence of multiple PIP₂-phosphatases have been implicated in the negative regulation of PIP₂-dependent PLD activity within flounder brain.
구희정(Hee Jung Ku),서정길(Jung Kil Seo),김은희(Eun Hi Kim),허민도(Min Do Huh),정준기(Joon Ki Chung),박장수(Jang Su Park),강신원(Shin Won Kang),박남규(Nam Gyu Park) 한국수산과학회 1997 한국수산과학회지 Vol.30 No.2
The relationship between structure and biological activity was studied on the three neuropeptides (mammalian-, trout- and goldfish-neuropeptide γ) that were syntheized by the solid-phase method. Circular dichroism spectra showed that mammalian-, trout- and goldfish-neuropeptide γ adopted an unordered structure in buffer solution. In the presence of neutral and acidic liposomes, mammalian- and trout-neuropeptide γ also took a random structure. However, goldfish-neuropeptide γ took an α-helical structure in acidic liposomes. The intestinal motility response was investigated with carp intestines, guinea-pig ileums and rat duodenums. In case of carp intestine, contractile activity was as follows : goldfish-neuropeptide γ- trout-neuropeptide γ > mammalian-neuropeptide γ. On the other hand, the contractile activity of mammalian-neuropeptide γ was more potent than trout- and goldfish-neuropeptide γ in the guinea-pig ileums and rat duodenums. These results suggest that neuropeptide γs show the species-specific activity.
먹장어 (Eptatretus burgeri)의 피부로부터 Brabykinin-Related Peptide의 정제
신미정 ( Mi Jung Shin ),김은정 ( Eun Jung Kim ),김찬회 ( Chan Hee Kim ),고혜진 ( Hye Jin Go ),김인혜 ( In Hae Kim ),류홍수 ( Hong Soo Ryu ),허민도 ( Min Do Huh ),정준기 ( Joon Ki Chung ),박남규 ( Nam Gyu Park ) 한국수산과학회 2003 한국수산과학회지 Vol.36 No.1
Hagfish bradykinin (BK)-related peptide가 먹장어 (Eptatretus burgeri)의 피부추출물로부터 분리, 정제되어졌다. 분자량이 875.8 Da인 hagfish BK는 C_18 역상 및 양이온 교환 high performance liquid chromatography로 순수하게 정제되었다. 자동아미노산 서열 분석기와 MALDI-TOF mass spectroscopy에서 얻은 결과의 조합으로 hagfish BK의 일차구조는 Gly-Thr-Ala-Gly-Pro-Phe-Arg로 판명되었다. 이 아미노산서열을 mammalian BK와 비교하면 다섯개의 아미노산의 치환을 (Arg^1→Gly, Pro^2→Thr, Pro^3→Ala, Phe^5→Ile, Ser^6→Gly) 포함된다. Hagfish BK는 먹장어 장관에 대해 수축활성을 나타냈으며, 약 10^-11 M의 농도에서 역치반응이 나타났다. A hagfish bradykinin (BK)-related peptide was isolated and characterized from the skin of hagfish, Eptatretus burgeri. The hagfish BK with a molecular mass of 875.8 Da was purified to a homogeneity using C_12 reverse-phase and cation-exchange high performance liquid chromatography. The primary structure of the hagfish BK was determined as Gly-Thr-Ala-Gly-Pro-Phe-Arg by a combination of an automated amino acid sequencing and MALDI-TOF mass spectrometry. This amino acid sequence contains five substitutions (Arg^1→Gly, Pro^2→Thr, Pro^3→Ala, Phe^5→Ile, Ser^6→Gly) compared with that of mammalian BK. The hagfish BK showed a contractile action on the intestine of hagfish, Eptatretus burgeri. The threshold concentration of hagfish BK was around 10^-11M.
위전절제술 및 루프 식도공장 문합술을 시행한 환자와 정상인에서 Small Bowel Transit Time 의 비교
김선회(Sun Whe Kim),정준기(Joon Ki Jung),양한광(Han Kwang Yang),김진복(Jin Opk Kim),이명철(Myung chul Lee) 대한소화기학회 1988 대한소화기학회지 Vol.20 No.1
N/A There have been great advances in the operative technique and prevention of early postoperative complications of total gastrectomy. But much more efforts are needed to improve late postoperative complications, nutritional abnormalities after total gastrectoniy. There are many possible causes for malnutrition after total gastrectomy, such as poor oral intake, rnalabsorption due to bacterial proliferation in small bowel and relative pancreatic insufficiency etc. Changes in small bowel transit tirne can be considered as another candidate. The purpose of this study is to compare the small bowel transit time between fifteen patients who didnt show any evidence of recurrence after total gastrectomy and loop esophagojejunostomy due to gastric cancer and six normal volunteers who had no specific gastointestinal symptorns and signs. Fried egg-white which was tagged with 99mTc-SC, rice and water was used as test meal. The transit of the test meal was monitored up to 8 hours by gamma camera which was connected to a microcomputer. 1) The small bowel transit time of head of meal was 221+34.39 minutes in the patients after total gastrectonly and 232.5+26.26 minutes in the control subjects (P>0.05) 2) The small bowel transit time of midportion of meal was 326.0+58.04 minutes in the patiens after total gastrectoinay and 271.8+46.64 minutes in the control suuhjects (P >0.05) 3) There was a tendency of delayed transit time of the later part of meal in the patient group. There was no significant differences in small bowel transit time of head and midportion of meal between patients with weight loss after total gastrectomy ancl normal volunteers. Therefore weight loss as a manifestation of malnutritionn after total gastrectomy is not caused by changes in small bowel transit time.