천연 아로마 모기 기피제 (시트로넬라와 시트로넬롤)의 기피력 효과 측정

정은숙(Jung Eun-Suk),윤화경(Yun Hwa-Kyung) 한국산학기술학회 2005 한국산학기술학회 학술대회 Vol.- No.-

Repellent efficacies of two natural aroma compounds, citronella and citronellal, against mosquitoes were evaluated both in field and in vitro. In vitro, the experiment was conducted with three controlled bands impregnated with 30% citronella extract, 15% citronella extract and 30% citronellal extract, and with band impregnated 30% citronella in field. Raw data was obtained by the means of counting numbers bitten by mosquitoes per unit time, namely human bait method. Comparative repellent efficacies of above three controlled bands were calculated at 86%, 73%, and 78%, respectively in vitro, and 80% in field, showing high repellent effectiveness against mosquitoes. This estimation was also confirmed by t-test compared between control group and each experimental group.

연구논문 : 측백나무 잎 추출물의 미용 소재로서의 기능성 분석

정은숙 ( Eun Suk Jeong ),유민정 ( Min Jung Ryu ) 한국미용학회 2015 한국미용학회지 Vol.21 No.1

In this study, antioxidant (DPPH free radical scavenging activity, Nitric Oxide Scavenging activity), antimicrobial activities and bioactive materials (polyphenolics and flavonoids compounds) of ethanol extract of leaves from Thuja orientalis (TOL) were investigated. The ethanol extract from TOL showed higher DPPH radical scavenging activity and Nitric Oxide (NO) scavenging activity than other crude plant extract in a concentration-dependent manner. The present results suggest that the ethanol extract from TOL may be potent agent for antioxidant and thereby inhibit the pathological conditions caused by excessive generation of NO and ROS. Total phenolic content and Total flavonoid content was 61.2±2.35 and 42.32±1.015 μg/mL in ethanol extract of TOL respectively. The result of antimicrobialeffects, we were able to confirm that ethanol extract of TOL at S. epidermidis, p. acnes and M. furfur showed the clear zone on 250, 500 μg/disc concentration. The highest antimicrobial activity showed at p. ovale on 125, 250, 500 μg/disc concentration of ethanol extract of TOL. Taken together, ethanol extract of TOL was shown to have the most potent antioxidative and antimicrobial activities and the highest content of polyphenolic and flavonoids compounds.

DSP프로세서를 이용한 홍채영상인식 시스템구현에 관한 연구

김자환,정은숙,성경,류광렬,Kim, Ja-Hwan,Jung, Eun-Suk,Sung, Kyeong,Ryu, Kwang-Ryol 한국정보통신학회 2004 한국정보통신학회논문지 Vol.8 No.4

본 논문은 홍채영상 인식시스템의 처리 속도를 향상시키기 위하여 고성능 DSP 프로세서를 이용한 실시간 처리 시스템의 구현에 관한 연구이다. 시스템은 CCD 카메라, DSP 처리부, 네트워크와 연동을 위해 통신부로 구성된다. 시스템 구현 결과 홍채 영상의 인식 처리 속도가 0.9초 이하 정도 개선되었다. The iris image recognition system realization using DSP processor for the faster real-time processing is presented in this paper. The system is composed of CCD camera, DSP processing and network part to link the communication. The system based on high speed DSP processor leads the iris recognition processing time to be faster. The simulation results in 0.9sec below approximately.

열처리 방법에 따른 대두의 이화학적 특성 변화

김순희,정은숙,김소영,박신영,조용식,Kim, Sun Hee,Jung, Eun Suk,Kim, So Young,Park, Shin Young,Cho, Yong Sik 한국식품저장유통학회 2017 한국식품저장유통학회지 Vol.24 No.6

대두는 된장, 간장, 스낵 등 전통식품의 주요 원료이며, 열처리 공정은 대두의 가공과정에서 대부분 수반되는 단위 조작이다. 본 연구에서는 열처리가 대두의 이화학적 특성에 미치는 영향을 조사하기 위하여 상업적인 조건에서 대두를 증자, 가열팽화, 튀김 처리한 다음 단백질의 분자량 분포와 용해도, 수분과 유지 흡착력 및 열 특성과 산패도의 변화를 조사하였다. 대두는 가열처리에 의하여 단백질이 10-40 kDa 범위의 작은 분자량으로 분해되는 경향을 나타내었다. 대두의 용융 엔탈피는 199.62 J/g이었으며 열처리에 의하여 123.07-135.90 J/g 범위로 엔탈피가 감소하였고 지질 산화를 보여주는 fluorescence intensity도 열처리로 증가하였으며 열처리 효과는 튀김, 증자, 가열팽화의 순으로 높았다. 또한 대두의 수분 흡착력은 열처리 한 경우가 비열처리 대두보다 상대적으로 높았으며, 단백질의 용해도는 산성 영역(pH 3-6)에서 같은 경향을 보였다. 결과적으로 대두는 가열처리에 의하여 용융 엔탈피가 감소하고 단백질이 분해되며 지질 산화와 용해도 및 수분흡착력이 증가하는데 대두에 대한 열처리의 효과는 튀김 공정에서 뚜렷하였다. Soybean is one of the most common food materials for making traditional Korean foods such as soybean paste, soy source and soy snack, and their manufacturing processes include heat treatment of soybean. This study was carried out to investigate the effect of heat treatment on the physicochemical properties of soybean. All samples were heat treated under commercial steamed, puffed or air-fried conditions, and then the protein molecular weight distribution, thermal properties, fluorescence intensity, protein solubility, and water and oil holding ability of the heat treated soybeans were examined. Sodium dodecyl sulfate polyacrylamide gel electrophoresis indicated that heat treatment caused fragmentation of polypeptide chain in soybean, showing the band of low molecular ranging from 17 to 40 kDa. The differential scanning calorimetric analysis showed the decrease of enthalpy values (${\Delta}H$) by heat treatment. Fluorescence spectroscopy indicated that the heat treatment caused lipid oxidation as proved by increasing emission intensity. The protein solubility at pH 3-6, and water holding capacity of heat treated soybeans were the higher than no treatment. These results suggest that the heat treatment resulted in decreased enthalpy values, and increased protein degradation, lipid oxidation and water affinity of soybean. Moreover, the effect of heat treatment on physiochemical properties of soybeans was more significant under air-fried condition.

FIM을 사용한 뇌졸중 환자의 퇴원시 기능상태 평가에 관한 연구

홍순탁(Hong Sun-Tak),박형숙(Pak Hyeong-Suk),정은숙(Jung Eun-Suk) 한국재활간호학회 1998 재활간호학회지 Vol.1 No.1

구강관리 프로토콜 적용이 항암화학요법을 받는 고형암 환자의 구강상태 및 자가간호수행에 미치는 효과

정미숙(Mi Sook Jeong),송지은(Chi Eun Song),이애리(Ae Ri Lee),정은숙(Eun-Suk Jung),김광숙(Gwang Sug Kim) 한국콘텐츠학회 2018 한국콘텐츠학회논문지 Vol.18 No.8

본 연구의 목적은 항암화학요법을 받는 고형암 환자를 대상으로 구강관리 개별교육과 냉동요법 및 벤지다민 구강함수를 포함한 구강관리 프로토콜 적용이 대상자의 구강상태와 수행에 미치는 효과를 파악하기 위함이다. 총 연구 대상자는 31명이었으며, 실험군에 13명, 대조군에 18명이 배정되었다. 실험군에게는 구강관리교육, 냉동요법 및 벤지다민 구강함수를 적용하였으며 대조군은 기존의 구강간호를 제공하였고, 대상자의 구강상태와 자가간호 수행정도를 측정하였다. 수집된 자료는 Mann-Whitney test, Friedman test, 및 Chi-square test로 분석하였다. 연구결과, 매 측정 시기 별 두 그룹 간 구강상태의 차이는 없는 것으로 나타났다. 그러나 두 그룹 모두 측정시기에 따른 구강상태의 변화는 유의하였는데 항암치료 후 14일경에 구강상태점수가 가장 낮게 측정되었다. 자가간호 수행정도는 실험군이 대조군보다 유의하게 높았으며 연구 완료 후 4개월까지 유지되는 것으로 나타났다. 이러한 결과는 종양간호사에 의한 환자개별교육의 효과로 여겨지며 고형암 환자의 구강상태 개선을 위하여 냉동요법과 벤지다민 구강함수에 대한 효과를 파악하기 위한 추가적인 연구가 요구된다. The purpose of this study was to identify the effectiveness of oral care protocol including individual oral care education, cryotherapy and benzydamine gargling on oral cavity status and self-care performance in solid cancer patients receiving chemotherapy. Thirty-one patients were enrolled by convenience sampling in this study and allocated to control (n=18) and experimental group (n=13). In the intervention group, individual oral care education, oral cryotherapy and benzydamine gargling were applied, while the control group received pre-existed oral care. Oral cavity status and self-oral care behavior were measured in five periods. The data were analyzed using the Mann-Whitney test, Friedman test, and Chi-square test. There was no significant difference in oral cavity status between the groups. But the two groups showed the poorest oral cavity status on 14th day. Self-care behavior was significantly higher in the intervention group than the control group. This effect had maintained for 4months after completion of study. The results showed that individual oral care education by oncology nurse is effective to encourage patients to do self-care behavior and a further study is needed to explore the effect of cryotherapy and benzydamine gargling on oral cavity status of patient with solid tumor.

유아 흡연예방교육을 위한 교사 교육프로그램의 시범 적용 및 효과

김순환(Kim, Soonhwan),정혜욱(Chung, Hoewook),정은숙(Jung, Eun suk),하민경(Ha, Min Kyung) 한국육아지원학회 2017 육아지원연구 Vol.12 No.3

본 연구는 유아 흡연예방교육을 위해 시범 적용한 교사 교육프로그램의 효과를 검증하는 것을 목적으로 하였다. 연구의 대상은 전국 유아교육기관 교사 242명이며, 프로그램의 시범적용은 2016년 11월 1일부터 11월 25일에 실시되었다. 프로그램의 효과를 살피기 위해 사용된 설문지는 교사의 지식, 태도, 실천을 측정하는 문항으로 구성되어 있으며, 관련문헌을 바탕으로 연구진이 제작하였다. 연구결과를 살펴보면, 첫째, 교사 교육프로그램은 교사의 유아 흡연예방교육의 지식, 태도, 실천 수준을 모두 향상시켰다. 둘째, 프로그램의 효과를 교사의 개인변인별로 살펴보면, 교사 교육프로그램은 교사의 학력, 경력에 상관없이 지식, 태도, 실천 수준을 향상시켰다. 셋째, 교사의 기관변인별로 살펴보면, 교사 교육프로그램은 기관유형과 교사의 담당학급에 상관없이 지식, 태도, 실천의 수준을 향상시켰다. 본 연구의 결과는 개발된 교사 교육프로그램이 교사의 개인변인 및 기관변인과 상관없이 유아흡연예방교육에 대한 교사의 지식, 태도, 실천 수준 향상에 효과가 있음을 의미한다. 이를 통해 유아교사의 흡연예방교육에 대한 역량 증진을 위해 유아 흡연예방 교사 교육의 지속적인 시행이 필요함을 시사하였다. This study aimed to develop a teacher training program for smoking prevention education for young children and verify its effects. For the study, 242 early childhood teachers responded to a survey questionnaire which examines teachers’ knowledge, attitude, and practice for smoking prevention education. The results revealed that teachers’ knowledge, attitude and practice improved through the training program . Secondly, in terms of individual variables, teachers’ knowledge, attitude and practice improved regardless of their educational backgrounds and years of teaching experiences. Thirdly, in terms of institutional variables, teachers’ knowledge, attitude and practice improved regardless of the types of the institutions and the class levels in charge. The results of this study imply that teacher education is necessary for the effective operation of smoking prevention education for young children.

일단계 핵산 추출을 이용한 사람거대세포바이러스와 돼지내재레트로바이러스의 검출법

김정헌,정은숙,황응수 대한감염학회 2010 감염과 화학요법 Vol.42 No.1

Background: Xenotransplantation is thought to be one of the alternative methods to overcome the shortage of human organs for transplantation. Recipients should be immunosuppressed for graft survival, and thus, there is a need for developing diagnostic modality that can detect diverse infections originating from animals and recipients rapidly, in the early stage, and with high sensitivity using small volume of samples. This study was carried out to develop a fast, simple, and robust technique for the preparation of HCMV DNA and PERV RNA using small volume of samples. Materials and Methods: Nucleic acids were extracted from serially diluted samples with one step extraction method as well as with Qiagen kit. The presence of genomic DNA of human cytomegalovirus (HCMV) and porcine endogenous retrovirus (PERV) was detected by PCR and specific primer set, respectively. RNA of HCMV and PERV was extracted and then detected by RT-PCR and specific primer set, respectively. For absolute quantification of HCMV, standard curve was established by real time PCR. Results: HCMV DNA and PERV RNA were prepared from culture supernatant and cells for PCR or RT-PCR with one step extraction method. It was possible to extract both the DNA and RNA from the samples in about 20 minutes with one step extraction method in a single tube. HCMV and PERV could also be detected by PCR and one step extraction method, respectively. It was also good with small quantity samples. Conclusions: One step extraction method is simpler and faster method than other extraction methods when there are two types of DNA and RNA viruses in one sample. From these results, we could see that the one step extraction method could be very useful in detecting HCMV and PERV rapidly from the pig cells or organ transplanted recipients with a small amount of sample.

SNU 미니돼지 유래 돼지내재레트로바이러스의 시험관내 사람세포주에서의 증식부전

김정헌,정은숙,박정규,김상준,황응수 대한감염학회 2010 감염과 화학요법 Vol.42 No.3

Background: The presence of porcine endogenous retrovirus (PERV) has been considered as one of the main hurdles to transplant pig’s organs or tissues to human beings. There has been no report that PERV infection is associated with human diseases. Because pigs have their own characteristics of PERV according to pig strain, it is necessary to analyze the infectivity of PERV from SNU miniature pig to human cells for future utilization as a transplantation donor. Materials and Methods: Human cell lines were infected with culture supernatant from porcine cell line or immunomodulator-stimulated peripheral blood mononuclear cells (PBMC) of SNU miniature pigs. They were also co-cultured with PBMC or islet cells of SNU miniature pigs. The presence of PERV genes and general pig marker gene in cells was determined by nested PCR with primer set for PERV pol and pig mitochondrial cytochrome oxidase II (COII), respectively. Results: Infection test with the culture supernatant from PBMC of SNU miniature pigs showed that PERV pol but not COII was detected only in a few cases, but there was no uniform infection pattern in scope of stimulators and cell types. PERV pol was not demonstrated in co-cultures of human cell line with PBMC or islet cells from SNU miniature pigs after 80 days of co-cultures. Conclusions: In vitro infectivity test suggests that PERV from SNU miniature pig might not replicate productively in human cell lines although it could infect human cells and integrate into chromosome.

한국 조리식품의 칼슘과 인의 함량에 대한 고찰

김순덕,김정생,김학란,정은숙 효성여자대학교 가정대학 학도호국단 1985 家政大論集 Vol.4 No.-

This study was conducted to investigate the content of calcium and phosphorous andtheir blance in the korean foods which were composed of boiled rices (9 kinds), soups(34 kinds), pot stews (27 kinds), roasted foods (26 kinds), hard boiled foods (22kinds), jerkings (21 kinds) and seasoning (22 kinds) as side dishes. Boiled rice, boiled rice and cereals, and rice ball among 9 kinds of boiled rices wereextremely acidic food as their P/Ca ratio was higher than 5, but hash rice, fried rice,olio rice and mixed soup rice among them were lower than 2. In the soup5, only P/Ca ratio of young brocken soup among them was the range of3 to 4, and the ratio of chicken soup, shopped beef soup and the rest 31 kinds werelower than 2. In the pot stews, P/Ca ratio of ·soybean curd residue and shank pot stew were therange of 3 to 4, the rest 25 kinds were lower than 2. Among the 91 kinds of side dishes, the P/Ca ratio of roasted potato green pepper,hard boiled hot green pepper, hard hotted beef potato, hard boiled hair tail, roastedonion, grilled pumpkin, rolled egg fry and roasted mackerel pike were the range of-3 to 4. Extremely acidic foods above 4 were roasted chicken potato, roasted pork, roasted'common octopus, roasted anchovy, roasted thin strips cuttle fish, chicken pot stew,roasted pork potato, roasted pork, roasted pork with soy sauce, fried potato Onlon fried:chicken, fried pork potato, fried cuttle liver, fried pork, roasted spanish mackerel .ard iced thin strips of cuttle fish, and almost of the rests were lower than 2. As above results, there were characteristics which acid-base balance of korean foodswere not balanced independently, but could control the balance with compositions as amenu, while there were some unbalanced foods owing to calcium content which werecomposed boiled rice, meat soup and side dish prepared from meat.