위 평활근에서 비교감성 비콜린성 이완반응과 susbstance P에 의한 조절

장성종 ( Sung Jong Chang ),허광식 ( Gwang Sik Heo ),박찬국 ( Chan Guk Park ),김만우 ( Man Woo Kim ),전제열 ( Jae Yeoul Jun ) 대한소화기학회 2002 대한소화기학회 추계학술대회 Vol.2002 No.-

소장 Cajal 세포에서 발생되는 자발적 내향성전류에 대한 noradrenaline의 효과

허광식 ( Gwang Sik Heo ),장성종 ( Sung Jong Chang ),박찬국 ( Chan Guk Park ),김만우 ( Man Woo Kim ),전제열 ( Jae Yeoul Jun ) 대한소화기학회 2002 대한소화기학회 추계학술대회 Vol.2002 No.-

위 평활근에서 비교감성 비콜린성 이완반응과 Substance P에 의한 조절

허광식 ( Gwang Sik Heo ),장성종 ( Sung Jong Chang ),박찬국 ( Chan Guk Park ),김만우 ( Man Woo Kim ),장인엽 ( In Youb Jang ),김진호 ( Jin Ho Kim ),신무경 ( Moo Kyoung Shin ),염철호 ( Cheol Ho Yeum ),윤평진 ( Pyung Jin Yoon ),전제열 대한소화기학회 2003 대한소화기학회지 Vol.41 No.5

Background/Aims: In a gastric fundic strip, electrical field stimulation (EFS) evokes TTX-sensitive biphasic responses, consisting firstly of cholinergic contraction followed by a transient relaxation. It is well known that nonadrenergic noncholinergic (NANC) inhibitory nerve mediates a transient relaxation. This study was performed to investigate the characterization of relaxation and its modulation by substance P. Methods: Using Guinea-pig gastric fundic smooth muscle tissues, we recorded mechanical contractions induced by EFS in the organ bath with platinum. Results: NG-nitro-L-arginine methyl ester, an inhibitor of nitric oxide synthase, reduced EFS-induced relaxation and these effects were reversed by L-arginine, a precursor of nitric oxide (NO). Sodium nitroprusside, a NO-donor, suppressed the fundic basal tension. Cell permeable 8-bromo-cGMP inhibited noradrenaline-induced contraction. The application of substance P increased basal tension and EFS-induced contraction and relaxation. NK-1 receptor antagonist ([D-Pro9-(spiro--lactam)9,10-Trp11]-Substance P) inhibited substance P-induced effects. TEA and apamin, K+ channel blockers, increased basal tension and EFS-induced relaxation. Conclusions: These results indicate that NANC inhibitory responses are mainly mediated by NO in the guinea-pig fundus and the release of NO is modulated by substance P through NK-1 receptor and by prejunctional K+ channels. (Korean J Gastroenterol 2003;41:358-365)

생쥐 대장 평활근세포의 막 전압-의존 이온 전류에 대한 Tamoxifen효과

이동민 ( Dong Min Lee ),장성종 ( Sung Jong Chang ),박찬국 ( Chan Guk Park ),김만우 ( Man Woo Kim ),임건한 ( Gun Han Lim ),최석 ( Seok Choi ),염철호 ( Cheol Ho Yeum ),윤평진 ( Pyung Jin Yoon ),전제열 ( Jae Yeoul Jun ) 대한소화기학회 2005 대한소화기학회지 Vol.46 No.5

췌장의 가성낭종 파열에 의한 하부 위장관 출혈 1예

장성종,김병수,부귀범,김동규,박찬국,김만우,김정용,박상헌 조선대학교 부설 의학연구소 2000 The Medical Journal of Chosun University Vol.25 No.1

Pancreatic pseudocysts usually develop as a complication of acute pancreatitis or repeated attacks of chronic pancreatitis. Pseudocysts may also develop as a result of traumatic injuries or neoplasms. An uncommon complication is massive hemorrhage inside the pancreatic pseudocyst. The exact mechanism of hemorrhage is unknown. Enzymatic digestion, pressure erosion from the cyst mass or a combination of these processes may play an important role. Gastrointestinal hemorrhage associated with a pancreatic pseudocyst can occur in 10 percent of cases. When hemorrhage occurs because of erosion into a blood vessel involved in the pseudocyst, surgery is indicated since the episodes of hemorrhage are often severe and may even be fatal. When there is colonic involvement, various complications may occur. The most dangerous one is spontaneous rupture into the colon, which requires immediate surgical treatment because of high incidence of fulminating sepsis or massive hemorrhage. We examined a sixty eight-year-old man complaining of hematochezia and dizziness, and reported that it was a case of massive lower gastrointestinal hemorrhage caused by pseudocyst of the pancreas ruptured into the colon associated with chronic pancreatitis. On operative findings, the peripancreatic fatty tissue had severe adhesions to transverse colon and tail portion of pancreas, where the pseudocyst had formed. A distal pancreatectomy, splenectomy and resection of transverse colon were performed. By microscopic examination, hemorrhagic necrosis and inflammatory change were observed in the pancreas specimen. The tail portion of pancreas had a 6×6 cm sized cystic mass and was proven to be a pseudocyst with no epithelial lining.

위장관 출혈을 일으킨 공장의 형활근종 1예

김병수,장성종,부귀범,조운택,박찬국,김만우,변주남,김정용,전호종 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.1

Small bowel leiomyoma is a rare tumor which may occur wherever smooth muscle is present. The small bowel is the most common site and it makes up 20~30% of all benign small intestinal neoplasms. A spectrum of symptoms are common ranging from nausea, vomiting, abdominal pain, to massive intestinal bleeding. Bleeding is the most common symptom and is usually gross and recurrent. Occasionally it may be life threatening. But, the most serious problem is both the absence of specific clinical symptoms, and the difficulties shown in radiological diagnostics of the small bowel. If conventional diagnostic methods fail to reveal any positive findings for gastrointestinal bleeding, the diagnosis of intestinal leiomyoma should be suspected and an SMA angiography shoud be performed. The angiographic criteria of small bowel leiomyoma is very characteristic. We present a case of leiomyoma with considerable and prolonged gastrointestinal bleeding, which was diagnosed by angiography. A 61-year old woman was admitted with a history of melena over the last 10 days. An SMA angiography showed a hypervascular mass with a diameter of 3×3cm. A segmental resection was performed under the impression of the gastrointestinal bleeding due to leiomyoma of the small bowel. Microscopic examination of the lesion shows interlacing or whorl bundles of spindle shaped ces and no mitosis.

급성 지주막하 출혈에 동반된 관상동맥 연축에 의한 심근경색증 1예

부귀범,박근호,양종태,이동민,장성종,김건영,장경식 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.1

In many cases, acute cerebrovascular disease is accompanied by abnormal electocardiograms. Also, though rather uncommon, acute myocardial infraction is also seen. The mechanism of its occurance is uncertain but it is thought to be related to coronary artery spasms, an abnormal autonomic nervous system, and a catecholamine increase. A female patient of 34 years of age came to the hospital because of sudden headache accompanied by substernal chest pain. A subarachnoid hemorrhage due to a cerebral aneurysm rupture had occured, and there was ST segment elevation in lead II, III, and aVF of the electrocardiogram. A cardiac enzyme test, an echocardiography, and a coronary angiography were performed, and she was diagnosed to have acute myocardial infarction due to coronary artery spasm. Clipping of the aneurysm was performed. With the use of nitrate and an angiotension converting enzyme inhibitor, the condition of the patient improved.

급성 심근 경색증과 혈장 Paraoxonase activity와의 관계

김건영,윤상준,박근호,조영신,장성종,장경식,홍순표 조선대학교 부설 의학연구소 2000 The Medical Journal of Chosun University Vol.25 No.1

연구배경: 혈장 파라옥소나제의 감소는 HDL-cholesterol의 항 산화작용의 중요한 요소로 알려져 있어 이의 감소는 항산화작용의 저하로 인해 동맥경화의 진행을 촉진시킬 수 있어 허혈성심장병의 주요 위험인자로 추정하고 있다. 본 연구에서는 급성 심근 경색증 환자를 대상으로 혈장 파라옥소나제의 활성도를 측정하여 정상군과 비교하여 혈장 파라옥소나제 활성도의 감소가 급성 심근 경색증의 위험인자인지 알아보고자 하였다. 대상 및 방법: 급성 심근 경색증으로 입원한 39 명의 환자(남자 : 여자 = 19 : 20, 평균나이: 61±4.3세)와 정상 대조군 36명(남자 : 여자 = 17 : 19, 평균나이: 55±10세)을 대상으로 정맥혈을 채혈 후 파라옥소나제 활성도와 LDL-cholesterol, total cholesterol, HDL-cholesterol를 측정하였다. 두 군간의 비교는 Student T-test를 이용하였다. 결과: 건강 대조군과 급성 심근 경색 환자군 간에 측정치를 비교해본 결과 혈장 콜레스테롤 수치는 182.10± 12mg/dl, 194.87± 40mg/dl이고 HDL 콜레스테롤은 54±5.4mg/dl, 37.52± 13mg/dl 였다. LDL 콜레스테롤은 94.45±13mg/d1, 122±34mg/d1로 급성 심근 경색증 환자의 경우 통계학적으로 의미있는 차이를 보였다. (P<0.05). 그러나 혈장 파라옥소나제 활성도는 건강 대조군 112.35±15U/ml, 급성심근 경색증 환자군 96.10±43.50U/ml로 급성 심근 경색증 환자군이 건강 대조군에 비해 낮은 수치를 보이는 경향이 있었으나 통계학적으로 유의한 차이(P=0.071)는 보이지 않았다(Table 2참조). Background : Human plasma paraoxonase is a HDL-associated ester hydrolase that catalyzes the hydrolysis of organophosphates, aromatic carboxylic acid esters and carbamates. HDL has been implicated in the prevention of LDL lipid peroxidation. Low paraoxonase activity in the Plasma may have responsibility to the development of coronary artery disease partially because oxidized LDL has intimate relationship with atherosclerosis. We studied plasma paraoxonase activities in the plasma of acute myocardial infarction patients in order to evaluate the relationship between these enzyme activities and the acute myocardial infarction. Materials and Methods : plasma samples were obtained from 39 individuals with acute myocardial infarction(M: 19 cases, F: 20cases, mean age: 61(4.3 years) and 36 individuals of healthy control(M: 17 cases, F: 19cases, mean age: 55(10 years). We determined paraoxonase activities, total cholesterol, LDL-cholesterol &DL-cholesterol levels in the plasma samples. Paraoxonase activities were measured spectrophoto metrically in 0.1M Tris-HCl buffer (pH=7.4) at 25℃ with paraoxon as substrate(5.5mM) at 405 nm. We used student T-test to compare the results of both groups. Results : Comparison of the results between myocardial infarction patients and normal control groups revealed that plasma total cholesterol, HDL-cholesterol and LDL-cholesterol levels showed statistically significant differences between groups but plasma paraoxonase activity does not. Conclusion : Our study showed that there was no significant difference of plasma paraoxonase activity between acute myocardial infarction and normal population. So low plasma paraoxonase activity may not be a risk factor of acute myocardial infarction. but further prospective large scale study will be needed to conclude.

생쥐 대장 평활근에서 막전압-의존성 이온 전류에 대한 tamoxifen 효과

박찬국,허광식,장성종,김만우,윤영,박도영,양경철,신무경,차경훈,염철호,윤평진,전제열 조선대학교 2003 The Medical Journal of Chosun University Vol.28 No.1

Background and Objectives : Tamoxifen is a widely used anticancer drug for breast cancer causing frequent gastrointestinal side effects. The change of gastrointestinal motility is associated with the alteration of activities of membrane ion channels. The ion channel is found to play an important role in regulating membrane potential and cell excitability. This study was performed to investigate the effects of tamoxifen on the membrane ionic currents in colonic myocytes. Materials and Methods : Single colonic myocytes were isolated by using a collganase from murine proximal colon. The membrane currents were recorded by using a whole-cell patch clamp method at room temperature. Results : Two types of voltage-dependent K+ currents (A-type and delayed rectifier K+ currents) were recorded. Tamoxifen inhibited both types of voltage-dependent K+ currents (10 μM). However, tamoxifen did not change the half-inactivation potential and the recovery time of voltage-dependent K+ currents. Chelerythrine, a protein kinase C inhibitor, and phorbol-12,13-dibutyrate, a protein kinase C activator, did not affect the voltage-dependent K+ currents Guanosine 5-O-2-thiodiphosphate (GDPβS, a G-protein inhibitor), also did not affect on tamoxifen-induced inhibition of voltage-dependent K+ currents. In audition, tamoxifen completely inhibited the voltage-dependent Ca²+ currents in whole-test ranges. Conclusion : These results suggest that tamoxifen inhibits the voltage-dependent membrane ionic currents in colonic smooth muscle cells, and that this action may be relevant to the gastrointestinal side effects

제2형 당뇨병 환자에서의 혈중 렙틴 농도

김현리,배학연,장성종,김희중,정재용,김양수,김태균,박유환,정춘해 조선대학교 부설 의학연구소 1999 The Medical Journal of Chosun University Vol.24 No.2

Objective: Leptin, the product of obesity (ob) gene, is a 16-kDa peptide hormone secreted by adipocytes and is thought to be a homeostatic signal that contributes to body weight regulation through modulating feeding behavior and/or energy expenditure. Mutations of the ob gene that led to leptin deficiency or production of a truncated inactive protein are associated with hyperphagia, hypometabolism, obesity, and noninsulindependent diabetes mellitus(NIDDM) in obese ob/ob mice. Leptin' s role in humans with obesity and NDDM is not known. Plasma leptin concentrations are shown to be elevated in obese humans. We questioned whether subjects with NIDDM have an altered regulation of serum leptin levels. Therefore, the object of this study is to evaluate the relationships between the serum leptin level and BMI, NIDDM. Method: This study were made of 70 subjects(control: 35, NIDDM:35) at Chosun University Hospital in Kwangju from March, 1998 to September, 1998. We measured the height and weight for BMI. Also, we measured leptin and C-peptide through radioimmunoassay. Result: The serum leptin concentration were not different in patients with type II diabetic and nondiabetic subjects (6.3 0.86/7.22 0.96, p=0.476) and the BMI was similar in diabetic and nondiabetic subjects(24 0.57/24 0.45, p=0.93). The leptin concentration were higher in women than in men regardless of diabetic status (diabetes: male 3.07 0.40 / female 8.20 1.18, p<0.05 control: male 4.88 1.28 / female 8.60 1.25, p<0.05). Conclusion: We concluded that the leptin concentrations were not different in diabetic and nondiabetic subjects and that the association of leptin with sexual dimorphism was similar in diabetic and nondiabetic subjects.